Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 2,154,691 C→T A242A (GCC→GCT)  unknown → Integral membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*12,154,6910CT96.5% 90.3 / ‑5.9 29A242A (GCC→GCT) unknownIntegral membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (15/13);  minor base G (0/1);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACAAGTAGCGAAAAGCGGCGGCGCATTCCCAACAACCTTGTTTATCGCGTTCTCATTGGCAGCCTGCATTATGTTCATTTATTTGCTGTTTAAACAAACCAAATGGAATATAGAGAGCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCCGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGGTATTGTATTTAGCTTAACGGCAATCTTTTGTTTGTATTATTAGATAAAATTTTGGCTTAGATTTGAGAAAAATGATGGCAAAAAACG  >  1/2154471‑2154864
                                                                                                                                                                                                                            |                                                                                                                                                                             
aaCAAGTAGCGAAAAGCGGCGGCGCATTCCCAACAACCTTGTTTATCGCGTTCTCATTGGCAGCCTGCATTATGTTCATTTATTTGCTGTTTAAACAAACCAAATGGAATATAGAGAGCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAatat                                                                                                                                                                    <  1:220424/232‑1 (MQ=255)
   aaGTAGCGAAAAGCGGCGGCGCATTCCCAACAACCTTGTTTATCGCGTTCTCATTGGCAGCCTGCATTATGTTCATTTATTTGCTGTTTAAACAAACCAAATGGAATATAGAGAGCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCAc                                                                                                                                               >  1:180299/1‑250 (MQ=255)
                                   acCTTGTTTATCGCGTTCTCATTGGCAGCCTGCATTATGTTCATTTATTTGCTGTTTAAACAAACCAAATGGAATATAGAGAGCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAAt                                                                                                              >  1:647461/1‑251 (MQ=255)
                                                  ttCTCATTGGCAGCCTGCATTATGTTCATTTATTTGCTGTTTAAACAAACCAAATGGAATATAGAGAGCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAaattaatt                                                                                                <  1:396958/250‑1 (MQ=255)
                                                      cATTGGCAGCCTGCATTATGTTCATTTATTTGCTGTTTAAACAAACCAAATGGAATATAGAGAGCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTa                                                                                           >  1:413799/1‑251 (MQ=255)
                                                                         gTTCATTTATTTGCTGTTTAAACAAACCAAATGGAATATAGAGAGCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGgtattgta                                                                                  >  1:84249/1‑241 (MQ=255)
                                                                              tttatttGCTGTTTAAACAAACCAAATGGAATATAGAGAGCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGGTATTGTATTTAGCTTAACGGCa                                                                   >  1:713406/1‑251 (MQ=255)
                                                                                          ttAAACAAACCAAATGGAATATAGAGAGCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAat                                                                                                                                                                      >  1:299529/1‑140 (MQ=255)
                                                                                                  acCAAATGGAATATAGAGAGCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGGTATTGTATTTAGCTTAACGGCAATCTTTTGTTTGTAttatta                                               <  1:401330/251‑1 (MQ=255)
                                                                                                   ccAAATGGAATATAGAGAGCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGGTATTGTATTTAGCTTAACGGCAATCTTTTGTTTGTATTATTAg                                              <  1:178295/251‑1 (MQ=255)
                                                                                                     aaaTGGAATATAGAGAGCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGGTATTGTATTTAGCTTAACGGCAATCTTTTGTTTGTATTATTAGAt                                            >  1:365962/1‑251 (MQ=255)
                                                                                                         ggAATATAGAGAGCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGGTATTGTATTTAGCTTAACGGCAATCTTTTGTTTGTattatt                                                <  1:124662/243‑1 (MQ=255)
                                                                                                         ggAATATAGAGAGCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGGTATTGTATTTAGCTTAACGGCAATCTTTTGTTTGTATTATTAGATaaaa                                        >  1:891907/1‑251 (MQ=255)
                                                                                                          gAATATAGAGAGCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCgg                                                                                                                                      <  1:346206/156‑1 (MQ=255)
                                                                                                          gAATATAGAGAGCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGgtattgtat                                                                                 >  1:275701/1‑209 (MQ=255)
                                                                                                                 gagagCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGGTATTGTATTTAGc                                                                            <  1:552761/207‑1 (MQ=255)
                                                                                                                     gCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGGTATTGTAttt                                                                               >  1:37092/1‑200 (MQ=255)
                                                                                                                        ttGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGgtattgtat                                                                                 >  1:888451/1‑195 (MQ=255)
                                                                                                                                     tattTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGgtattgtat                                                                                 <  1:227842/182‑1 (MQ=255)
                                                                                                                                               gtgCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGGTATTGTATTTAGCTTAACGGCAATCTTTTGTTTGTATTATTAGATAAAATTTTGGCTTAGATTTGAGAAAAATGATGGCAAAAAACg  >  1:188027/1‑251 (MQ=255)
                                                                                                                                                 gCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGgtattgtat                                                                                 <  1:238491/170‑1 (MQ=255)
                                                                                                                                                              aaTATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCgg                                                                                                                                                                 <  1:734411/77‑1 (MQ=255)
                                                                                                                                                                           aCATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAAt                                                                                                              >  1:28651/1‑115 (MQ=255)
                                                                                                                                                                            cATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGgtattgtat                                                                                 >  1:376803/1‑143 (MQ=255)
                                                                                                                                                                            cATTAAAGCGCATCAAAGTTTTGACTCTAATCCAACGTTGGTTTTTTCGGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGGTATTGTAttt                                                                               <  1:252738/145‑1 (MQ=255)
                                                                                                                                                                                gaaGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCAGTGTAAttt                                                                                                                                                         <  1:34812/66‑1 (MQ=255)
                                                                                                                                                                                           aaGTTTTGGCTCTAATCCAACGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGGTATTGTATTTAGCTTAACGGCAATCTTTTGTTTGTATTATTAg                                              <  1:636576/163‑1 (MQ=255)
                                                                                                                                                                                                             aaCGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGGTATTGTATTTAGCTTAACGGCAATCTTTTGTTTGTATTATTAGATAAAATTTTGGCTTAGATTTgaga                     >  1:377419/1‑170 (MQ=255)
                                                                                                                                                                                                              aCGTTGGTTTTTGCTGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCAATAATTTTTAAAGAGAAAATAACCa                                                                                                         <  1:730485/85‑1 (MQ=255)
                                                                                                                                                                                                                            |                                                                                                                                                                             
AACAAGTAGCGAAAAGCGGCGGCGCATTCCCAACAACCTTGTTTATCGCGTTCTCATTGGCAGCCTGCATTATGTTCATTTATTTGCTGTTTAAACAAACCAAATGGAATATAGAGAGCTTTGTAGGCGGCATTATTTTAGGTGTGCTGAATTTCTTTAATATTTTGTTTTACATTAAAGCGCATCAAAGTTTTGGCTCTAATCCAACGTTGGTTTTTGCCGGTATGAATATCGGTGTAATTTGTTTAGGCACCTTCACCGGCGCATTAATTTTTAAAGAGAAAATAACCAAAATTAATTGGTTAGGTATTGTATTTAGCTTAACGGCAATCTTTTGTTTGTATTATTAGATAAAATTTTGGCTTAGATTTGAGAAAAATGATGGCAAAAAACG  >  1/2154471‑2154864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑