Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	380,716	A→G	T67A (ACC→GCC)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	380,716	0	A	G	100.0%	280.0 / NA	81	T67A (ACC→GCC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/40);  total (41/40)

TTTTTAAACCTTACGATTAGGAGAATACCTATGTTTGCTTGGTTAAAACCAAAATTTGTGACATTACTTTGTGCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTACCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAA  >  1/380488‑380954                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               tttttAAACCTTACGATTAGGAGAATACCTATGTTTGCTTGGTTAAAACCAAAATTTGTGACATTACTTTGTGCACTTTTGTTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCggg                                                                                                                                                                                                                          <  1:453029/251‑1 (MQ=255) tttttAAACCTTACGATTAGGAGAATACCTATGTTTGCTTGGTTAAAACCAAAATTTGTGACATTACTTTGTGCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCggg                                                                                                                                                                                                                          >  1:675684/1‑251 (MQ=255)        aCCTTACGATTAGGAGAATACCTATGTTTGCTTGGTTAAAACCAAAATTTGTGACATTACTTTGTGCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACg                                                                                                                                                                                                                    <  1:794853/250‑1 (MQ=255)         ccTTACGATTAGGAGAATACCTATGTTTGCTTGGTTAAAACCAAAATTTGTGACATTACTTTGTGCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACg                                                                                                                                                                                                                    >  1:763731/1‑249 (MQ=255)              cGATTAGGAGAATACCTATGTTTGCTTGGTTAAAACCAAAATTTGTGACATTACTTTGTGCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGaa                                                                                                                                                                                                             <  1:778828/251‑1 (MQ=255)                aTTAGGAGAATACCTATGTTTGCTTGGTTAAAACCAAAATTTGTGACATTACTTTGTGCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAAt                                                                                                                                                                                                            <  1:570171/250‑1 (MQ=255)                    ggAGAATACCTATGTTTGCTTGGTTAAAACCAAAATTTGTGACATTACTTTGTGCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACACTCCGCGGGAAAACGCTTGGAATTGAg                                                                                                                                                                                                        >  1:485142/1‑250 (MQ=255)                    ggAGAATACCTATGTTTGCTTGGTTAAAACCAAAATTTGTGACATTACTTTGTGCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAg                                                                                                                                                                                                        >  1:793714/1‑250 (MQ=255)                         aTACCTATGTTTGCTTGGTTAAAACCAAAATTTGTGACATTACTTTGTGCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATcc                                                                                                                                                                                                                               >  1:472700/1‑222 (MQ=255)                                tGTTTGCTTGGTTAAAACCAAAATTTGTGACATTACTTTGTGCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGa                                                                                                                                                                                            >  1:420258/1‑250 (MQ=255)                                tGTTTGCTTGGTTAAAACCAAAATTTGTGACATTACTTTGTGCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGAc                                                                                                                                                                                           <  1:339132/251‑1 (MQ=255)                                tGTTTGCTTGGTTAAAACCAAAATTTGTGACATTACTTTGTGCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGAGAAAACGCTTGGAATTGAGCGGCATTATCGa                                                                                                                                                                                            >  1:536238/1‑250 (MQ=255)                                          gTTAAAACCAAAATTTGTGACATTACTTTGTGCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTc                                                                                                                                                                                  <  1:637573/250‑1 (MQ=255)                                                aCCAAAATTTGTGACATTACTTTGTGCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTg                                                                                                                                                                            >  1:340080/1‑250 (MQ=255)                                                       tttGTGACATTACTTTGTGCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAGTCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGa                                                                                                                                                                    <  1:321996/251‑1 (MQ=255)                                                             aCATTACTTTGTGCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGc                                                                                                                                                                                        >  1:878129/1‑225 (MQ=255)                                                             aCATTACTTTGTGCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGtt                                                                                                                                                              >  1:739186/1‑251 (MQ=255)                                                                       gtgCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCaaa                                                                                                                                                    >  1:72902/1‑251 (MQ=255)                                                                              tttGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAAt                                                                                                                                              >  1:768169/1‑250 (MQ=255)                                                                                      ccTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCtt                                                                                                                                      >  1:838961/1‑250 (MQ=255)                                                                                         cAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGac                                                                                                                                  <  1:150705/251‑1 (MQ=255)                                                                                           aCATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGacac                                                                                                                                <  1:468445/251‑1 (MQ=255)                                                                                           aCATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGaca                                                                                                                                 >  1:840626/1‑250 (MQ=255)                                                                                            cATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATAACAGCCTGCACGATTTCAGTGCCAGAATCGGCCAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCTACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGaca                                                                                                                                 >  1:809726/1‑249 (MQ=255)                                                                                                aTCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGacac                                                                                                                                >  1:228219/1‑246 (MQ=255)                                                                                                   aaCATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCtt                                                                                                                        <  1:286967/251‑1 (MQ=255)                                                                                                   aaCATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCtt                                                                                                                        <  1:621354/251‑1 (MQ=255)                                                                                                     cATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCttat                                                                                                                      <  1:868749/251‑1 (MQ=255)                                                                                                     cATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCttat                                                                                                                      <  1:479641/251‑1 (MQ=255)                                                                                                     cATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCttat                                                                                                                      <  1:28869/251‑1 (MQ=255)                                                                                                     cATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCAAAATTGAATCTCACCTTCGACACTGTGCCttat                                                                                                                      <  1:570890/251‑1 (MQ=255)                                                                                                       tCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCttatta                                                                                                                    <  1:745245/251‑1 (MQ=255)                                                                                                           cATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCttatta                                                                                                                    >  1:130000/1‑247 (MQ=255)                                                                                                           cATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTatga                                                                                                                 <  1:169608/250‑1 (MQ=255)                                                                                                           cATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTatga                                                                                                                 >  1:719595/1‑250 (MQ=255)                                                                                                           cATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTatga                                                                                                                 <  1:693500/250‑1 (MQ=255)                                                                                                           cATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTatga                                                                                                                 <  1:269672/250‑1 (MQ=255)                                                                                                                gCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGa                                                                                                                                                                    >  1:552747/1‑194 (MQ=255)                                                                                                                gCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCttatta                                                                                                                    <  1:376653/242‑1 (MQ=255)                                                                                                                       ccATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAgg                                                                                                     <  1:356817/250‑1 (MQ=255)                                                                                                                                       aaTCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAgg                                                                                    <  1:381754/251‑1 (MQ=255)                                                                                                                                          cAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTtattat                                                                                                                   >  1:259829/1‑217 (MQ=255)                                                                                                                                            aCCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCttatta                                                                                                                    >  1:124602/1‑214 (MQ=255)                                                                                                                                             ccAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATAtt                                                                               >  1:350795/1‑250 (MQ=255)                                                                                                                                             ccAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATAtt                                                                               >  1:161116/1‑250 (MQ=255)                                                                                                                                                            gCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTc                                                                                                                                                                                  <  1:438495/136‑1 (MQ=255)                                                                                                                                                            gCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGt                                                               >  1:205460/1‑251 (MQ=255)                                                                                                                                                               caATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGc                                                                                                                           <  1:822021/188‑1 (MQ=255)                                                                                                                                                                     ttATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGAc                                                                                                                                                                                                                                    >  1:166879/1‑77 (MQ=255)                                                                                                                                                                           aaaTAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAAc                                                <  1:501044/251‑1 (MQ=255)                                                                                                                                                                            aataatTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTatga                                                                                                                 >  1:794310/1‑185 (MQ=255)                                                                                                                                                                             ataatTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACaa                                              <  1:253331/251‑1 (MQ=255)                                                                                                                                                                              taatTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCttatta                                                                                                                    <  1:603146/180‑1 (MQ=255)                                                                                                                                                                              taatTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTatgatg                                                                                                               >  1:737013/1‑185 (MQ=255)                                                                                                                                                                              taatTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAAt                                             >  1:202557/1‑251 (MQ=255)                                                                                                                                                                              taatTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAAt                                             >  1:671067/1‑251 (MQ=255)                                                                                                                                                                              taatTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCACCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAAt                                             >  1:653017/1‑251 (MQ=255)                                                                                                                                                                                atTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAgg                                                                                    >  1:175481/1‑210 (MQ=255)                                                                                                                                                                                atTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATAc                                           <  1:652695/251‑1 (MQ=255)                                                                                                                                                                                  tATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACAt                                         <  1:111397/251‑1 (MQ=255)                                                                                                                                                                                  tATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTCTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACAt                                         <  1:335419/251‑1 (MQ=255)                                                                                                                                                                                   aTGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGttt                                                                                                                                                             <  1:336769/134‑1 (MQ=255)                                                                                                                                                                                    tGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACAt                                         >  1:251711/1‑249 (MQ=255)                                                                                                                                                                                     ggCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATggg                                      >  1:390908/1‑251 (MQ=255)                                                                                                                                                                                      gCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATggg                                      <  1:734045/250‑1 (MQ=255)                                                                                                                                                                                             cGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATAc                                           >  1:64046/1‑238 (MQ=255)                                                                                                                                                                                                   tGCCAGCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTc                        <  1:870590/251‑1 (MQ=255)                                                                                                                                                                                                        gCGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATAc                                           <  1:520156/227‑1 (MQ=255)                                                                                                                                                                                                         cGTGGATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCttatta                                                                                                                    <  1:426422/153‑1 (MQ=255)                                                                                                                                                                                                            ggATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCttat                                                                                                                      >  1:388565/1‑148 (MQ=255)                                                                                                                                                                                                            ggATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGc                                                                   <  1:618705/199‑1 (MQ=255)                                                                                                                                                                                                            ggATTACAGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGt               >  1:529774/1‑251 (MQ=255)                                                                                                                                                                                                                   aGTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCa         >  1:853492/1‑250 (MQ=255)                                                                                                                                                                                                                    gTCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCAc        >  1:358111/1‑250 (MQ=255)                                                                                                                                                                                                                     tCTGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTc          <  1:357147/247‑1 (MQ=255)                                                                                                                                                                                                                       tGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACcaac    <  1:845221/251‑1 (MQ=255)                                                                                                                                                                                                                       tGCACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACcaac    <  1:291346/251‑1 (MQ=255)                                                                                                                                                                                                                         cACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCAcc                                                                                                                                        >  1:437493/1‑117 (MQ=255)                                                                                                                                                                                                                         cACGATTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACcaacaa  <  1:22054/251‑1 (MQ=255)                                                                                                                                                                                                                             aTTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACaa                                              >  1:703946/1‑203 (MQ=255)                                                                                                                                                                                                                             aTTTCAGTGCCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTAc            >  1:135227/1‑237 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               TTTTTAAACCTTACGATTAGGAGAATACCTATGTTTGCTTGGTTAAAACCAAAATTTGTGACATTACTTTGTGCACTTTTGCTGACCTCAACATTATCAACATCCGCATTTGCCCTTGCCATCGGCGAAAATGAAATCAACCAATTGCTGGCTCGGCACAATAATTATAAAAATAATTATGGCATCCCCGGTCTTGCCAGCGTGGATTACAGTCTGCACGATTTCAGTACCAGAATCGGACAATCCGCGGGAAAACGCTTGGAATTGAGCGGCATTATCGACGGCTTATTCAAACTGCCTACCGATCAGTTTTCCGCCAAATTGAATCTCACCTTCGACACTGTGCCTTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAA  >  1/380488‑380954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑