Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,080,918	T→C	I107V (ATA→GTA)	unknown ←	Cytochrome c‑type biogenesis protein CcdA homolog, associated with MetSO reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,080,918	0	T	C	100.0%	218.0 / NA	62	I107V (ATA→GTA)	unknown	Cytochrome c‑type biogenesis protein CcdA homolog, associated with MetSO reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/29);  total (33/29)

GTGCTTTGGTGCGTTTTAGTAACTCTTGTGAGAATAAAGAGAAGAGTACAAACGGCGTTGCCAAACCTAATACATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTATTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGCCGAAATAAATCGGGATAATGGG  >  1/2080684‑2081161                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    gTGCTTTGGTGCGTTTTAGTAACTCTTGTGAGAATAAAGAGAAGAGTACAAACGGCGTTGCCAAACCTAATACATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGtt                                                                                                                                                                                                                                     >  1:132770/1‑251 (MQ=255)                    tAACTCTTGTGAGAATAAAGAGAAGAGTACAAACGGCGTTGCCAAACCTAATACATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAAt                                                                                                                                                                                                                   <  1:763627/250‑1 (MQ=255)                                    aaaGAGAAGAGTACAAACGGCGTTGCCAAACC‑‑‑TACATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTc                                                                                                                                                                                                                                    >  1:161045/1‑214 (MQ=255)                                          aagagTACAAACGGCGTTGCCAAACCTAATACATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATgg                                                                                                                                                                                              <  1:7232/249‑1 (MQ=255)                                              gTACAAACGGCGTTGCCAAACCTAATACATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCt                                                                                                                                                                                                       <  1:465854/236‑1 (MQ=255)                                              gTACAAACGGCGTTGCCAAACCTAATACATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATgg                                                                                                                                                                                              <  1:678164/245‑1 (MQ=255)                                                        cGTTGCCAAACCTAATACATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCa                                                                                                                                                                              <  1:365039/251‑1 (MQ=255)                                                                 aCCTAATACATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAactac                                                                                                                                                                                                                                            >  1:217705/1‑180 (MQ=255)                                                                        aCATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTTCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCaat                                                                                                                                                              <  1:430496/251‑1 (MQ=255)                                                                                       atAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTAt                                                                                                                                                >  1:626162/1‑250 (MQ=255)                                                                                        tAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAAtt                                                                                                                                                                                                   <  1:249889/198‑1 (MQ=255)                                                                                             gCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTaaa                                                                                                                                         >  1:462664/1‑251 (MQ=255)                                                                                               aCCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGa                                                                                                                                       <  1:674730/251‑1 (MQ=255)                                                                                                  gTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGAt                                                                                                                                                                          >  1:479943/1‑213 (MQ=255)                                                                                                  gTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATa                                                                                                                                    >  1:805417/1‑251 (MQ=255)                                                                                                  gTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATa                                                                                                                                    >  1:755430/1‑251 (MQ=255)                                                                                                  gTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATa                                                                                                                                    >  1:131658/1‑251 (MQ=255)                                                                                                  gTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATa                                                                                                                                    >  1:595463/1‑251 (MQ=255)                                                                                                       gagCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATGCAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCATTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCaataa                                                                                                                                                            <  1:248999/222‑1 (MQ=255)                                                                                                         gCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGc                                                                                                                              >  1:385909/1‑250 (MQ=255)                                                                                                                                cGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATAc                                                                                                                                                                                                         >  1:639583/1‑152 (MQ=255)                                                                                                                                cGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCCGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTa                                                                                                      >  1:442052/1‑251 (MQ=255)                                                                                                                                cGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATa                                                                                                                                                                         <  1:228972/184‑1 (MQ=255)                                                                                                                                cGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATc                                                                                                                     <  1:517070/236‑1 (MQ=255)                                                                                                                                  ccAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAgg                                                                                                    <  1:515793/251‑1 (MQ=255)                                                                                                                                     gAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATg                                                                                                                                                          <  1:505848/194‑1 (MQ=255)                                                                                                                                           aTGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATTCCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGa                                                                                             <  1:208938/249‑1 (MQ=255)                                                                                                                                           aTGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAg                                                                                           <  1:804925/251‑1 (MQ=255)                                                                                                                                             gCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGt                                                                                         >  1:3510/1‑251 (MQ=255)                                                                                                                                                       gTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGa                                                                                             >  1:108122/1‑237 (MQ=255)                                                                                                                                                       gTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATaaa                                                                               >  1:698078/1‑251 (MQ=255)                                                                                                                                                           aaTACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTc                                                                                                                                                                                          <  1:867622/140‑1 (MQ=255)                                                                                                                                                           aaTACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGAt                                                                                  >  1:78439/1‑244 (MQ=255)                                                                                                                                                            aTACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGt                                                                                         <  1:463287/236‑1 (MQ=255)                                                                                                                                                              aCAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCaa                                                                        <  1:358026/251‑1 (MQ=255)                                                                                                                                                                   gtgtCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGc                                                                                                                                                                     <  1:406188/153‑1 (MQ=255)                                                                                                                                                                     gtCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACaa                                                                 >  1:666404/1‑251 (MQ=255)                                                                                                                                                                           ccAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAggc                                                           <  1:308279/251‑2 (MQ=255)                                                                                                                                                                              aGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAAtt                                                                                                                                >  1:719636/1‑179 (MQ=255)                                                                                                                                                                               ggCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAAt                                                                                                                                 <  1:646822/177‑1 (MQ=255)                                                                                                                                                                               ggCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGa                                                                                             >  1:154482/1‑213 (MQ=255)                                                                                                                                                                               ggCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACaaaaa                                                              <  1:117058/244‑1 (MQ=255)                                                                                                                                                                               ggCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAgg                                                            <  1:812558/246‑1 (MQ=255)                                                                                                                                                                                gCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGaa                                                                                                                                                                                    <  1:212730/125‑1 (MQ=255)                                                                                                                                                                                   gAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGa                                                                                   <  1:706982/219‑1 (MQ=255)                                                                                                                                                                                   gAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATaa                                                   >  1:201167/1‑251 (MQ=255)                                                                                                                                                                                     aGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATg                                                                                                                                                          >  1:138680/1‑146 (MQ=255)                                                                                                                                                                                     aGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGcc                                                                                                        >  1:472216/1‑196 (MQ=255)                                                                                                                                                                                      ggTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTAACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAAttt                                                >  1:215570/1‑251 (MQ=255)                                                                                                                                                                                           gCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCg                                                                                                                                              <  1:162642/152‑1 (MQ=255)                                                                                                                                                                                           gCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTa                                           >  1:235894/1‑251 (MQ=255)                                                                                                                                                                                           gCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTa                                           >  1:873809/1‑251 (MQ=255)                                                                                                                                                                                                  gAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAAc                                                                              <  1:866549/209‑1 (MQ=255)                                                                                                                                                                                                  gAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCtt                                    >  1:638989/1‑251 (MQ=255)                                                                                                                                                                                                               aaaGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACg                                                                                              >  1:170449/1‑180 (MQ=255)                                                                                                                                                                                                               aaaGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGcc                       <  1:837812/251‑1 (MQ=255)                                                                                                                                                                                                                aaGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCaa                                                                                                                                                                                                                    <  1:852334/61‑1 (MQ=255)                                                                                                                                                                                                                        cTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAAcc                                                                             >  1:461713/1‑188 (MQ=255)                                                                                                                                                                                                                        cTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGCCGaaataaat              >  1:877462/1‑251 (MQ=255)                                                                                                                                                                                                                          tttGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGCCGAAATAAATCg            >  1:417415/1‑251 (MQ=255)                                                                                                                                                                                                                                    gTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAAc                                                                   >  1:851346/1‑186 (MQ=255)                                                                                                                                                                                                                                    gTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGCCGAAATAAATCGGGATAATggg  >  1:128471/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    GTGCTTTGGTGCGTTTTAGTAACTCTTGTGAGAATAAAGAGAAGAGTACAAACGGCGTTGCCAAACCTAATACATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTATTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGCCGAAATAAATCGGGATAATGGG  >  1/2080684‑2081161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑