Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,002,920	T→C	D145D (GAT→GAC)	unknown →	Chromosome segregation ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,002,920	0	T	C	100.0%	248.7 / NA	70	D145D (GAT→GAC)	unknown	Chromosome segregation ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/38);  total (32/38)

TCTTTGATTAAAGATGAGTTAAAAATTTATGAATCAGAAGATGCTTTATTATCAGTAGGTTTATATAAGCCGATGTTTAATTTTGATACTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGATTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGCTACTTATGAATATGCTTTGAAAAAAGAAGAGGAAAGGGCGGAGCAACTTAGAATTAAAG  >  1/2002675‑2003161                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  tCTTTGATTAAAGATGAGTTAAAAATTTATGAATCAGAAGATGCTTTATTATCAGTAGGTTTATATAAGCCGATGTTTAATTTTGATACTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGc                                                                                                                                                                                                                                              <  1:590299/251‑1 (MQ=255)                  gTTAAAAATTTATGAATCAGAAGATGCTTTATTATCAGTAGGTTTATATAAGCCGATGTTTAATTTTGATACTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATg                                                                                                                                                                                                                             >  1:65669/1‑251 (MQ=255)                                                attatCAGTAGGTTTATATAAGCCGATGTTTAATTTTGATACTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTaa                                                                                                                                                                                               >  1:249156/1‑251 (MQ=255)                                                   atCAGTAGGTTTATATAAGCCGATGTTTAATTTTGATACTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATg                                                                                                                                                                                            >  1:756952/1‑251 (MQ=255)                                                     cAGTAGGTTTATATAAGCCGATGTTTAATTTTGATACTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCt                                                                                                                                                                                                                                             <  1:804959/200‑1 (MQ=255)                                                          ggTTTATATAAGCCGATGTTTAATTTTGATACTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCt                                                                                                                                                                                                                                             >  1:559538/1‑195 (MQ=255)                                                          ggTTTATATAAGCCGATGTTTAATTTTGATACTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCg                                                                                                                                                                                                                          <  1:274080/214‑1 (MQ=255)                                                          ggTTTATATAAGCCGATGTTTAATTTTGATACTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACg                                                                                                                                                                                                      >  1:101502/1‑234 (MQ=255)                                                               atataAGCCGATGTTTAATTTTGATACTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATgc                                                                                                                                                                                                                >  1:701547/1‑219 (MQ=255)                                                               atataAGCCGATGTTTAATTTTGATACTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTg                                                                                                                                                                                >  1:52281/1‑251 (MQ=255)                                                               atataAGCCGATGTTTAATTTTGATACTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTg                                                                                                                                                                                >  1:69766/1‑251 (MQ=255)                                                                            tttAATTTTGATACTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGa                                                                                                                                                                    <  1:738738/250‑1 (MQ=255)                                                                                       tACTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGaaa                                                                                                                                                        <  1:575208/251‑1 (MQ=255)                                                                                       tACTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGaaa                                                                                                                                                        <  1:350731/251‑1 (MQ=255)                                                                                       tACTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGaaa                                                                                                                                                        <  1:512118/251‑1 (MQ=255)                                                                                       tACTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGaaa                                                                                                                                                        <  1:683720/251‑1 (MQ=255)                                                                                         cTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGaaa                                                                                                                                                        >  1:224990/1‑249 (MQ=255)                                                                                           tCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGaaa                                                                                                                                                                  >  1:861606/1‑237 (MQ=255)                                                                                                   gTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAgg                                                                                                                                                                                                                                       <  1:158249/160‑1 (MQ=255)                                                                                                   gTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATc                                                                                                                                                                             >  1:182741/1‑218 (MQ=255)                                                                                                    tataAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAg                                                                                                                                             <  1:686338/249‑1 (MQ=255)                                                                                                         aGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGt                                                                                                                                                                           <  1:4662/214‑1 (MQ=255)                                                                                                         aGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATc                                                                                                                                       >  1:600759/1‑250 (MQ=255)                                                                                                                aTTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCt                                                                                                                               <  1:495795/251‑1 (MQ=255)                                                                                                                 tttCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAg                                                                                                                                                                                                  <  1:513815/183‑1 (MQ=255)                                                                                                                                agaAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGt                                                                                                                <  1:424905/250‑1 (MQ=255)                                                                                                                                    gCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGa                                                                                                           >  1:405510/1‑251 (MQ=255)                                                                                                                                     cAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGaa                                                                                                          >  1:385595/1‑251 (MQ=255)                                                                                                                                           gAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGaaa                                                                                                                                                        >  1:798391/1‑199 (MQ=255)                                                                                                                                           gAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTcc                                                                                                                       >  1:606663/1‑232 (MQ=255)                                                                                                                                           gAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACa                                                                                                    >  1:122271/1‑251 (MQ=255)                                                                                                                                              ttATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAAcc                                                                                                 <  1:111472/251‑1 (MQ=255)                                                                                                                                                aTTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATcaacaa                                                                                                                                  >  1:841672/1‑216 (MQ=255)                                                                                                                                                                gCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATAtt                                                                                         <  1:882938/241‑1 (MQ=255)                                                                                                                                                                   tGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAAt                                                                            >  1:830570/1‑251 (MQ=255)                                                                                                                                                                    gTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTa                                                                                       <  1:296918/239‑1 (MQ=255)                                                                                                                                                                     tATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGTGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATAc                                                                                                     <  1:435896/224‑1 (MQ=255)                                                                                                                                                                     tATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTc                                                                          <  1:780551/251‑1 (MQ=255)                                                                                                                                                                           ttCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCGTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGc                                                                    <  1:443336/251‑1 (MQ=255)                                                                                                                                                                                 aTCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGaaa                                                                                                                                                        <  1:473257/161‑1 (MQ=255)                                                                                                                                                                                 aTCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGc                                                                                              >  1:630653/1‑219 (MQ=255)                                                                                                                                                                                 aTCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATgg                                                              >  1:552635/1‑251 (MQ=255)                                                                                                                                                                                  tCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGGCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGc                                                             <  1:437458/251‑1 (MQ=255)                                                                                                                                                                                    ttGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGCTa                                                           >  1:814219/1‑251 (MQ=255)                                                                                                                                                                                     tGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGCTAc                                                          <  1:830286/251‑1 (MQ=255)                                                                                                                                                                                      ggACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAag                                                                       >  1:29273/1‑237 (MQ=255)                                                                                                                                                                                      ggACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGCTa                                                           >  1:728518/1‑249 (MQ=255)                                                                                                                                                                                      ggACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGCTa                                                           >  1:702388/1‑249 (MQ=255)                                                                                                                                                                                        aCTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGCTACTTa                                                       <  1:887948/251‑1 (MQ=255)                                                                                                                                                                                           gTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGt                                                                                                                                                                                  >  1:305603/1‑125 (MQ=255)                                                                                                                                                                                           gTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATc                                                                                                                                                                             <  1:746343/130‑1 (MQ=255)                                                                                                                                                                                           gTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGa                                                                                                                                                                    <  1:604900/139‑1 (MQ=255)                                                                                                                                                                                           gTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATg                                                                                                                                               <  1:367346/160‑1 (MQ=255)                                                                                                                                                                                           gTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATg                                                                                                                          >  1:326381/1‑181 (MQ=255)                                                                                                                                                                                           gTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATc                                                                                   <  1:805366/220‑1 (MQ=255)                                                                                                                                                                                                gAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCt                                                                                                                                                                                                                                             <  1:405881/61‑1 (MQ=255)                                                                                                                                                                                                gAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATgg                                                                                                                                                                                                                            <  1:482178/78‑1 (MQ=255)                                                                                                                                                                                                 aaTGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGCTACTTATGAATATGc                                              <  1:685545/251‑1 (MQ=255)                                                                                                                                                                                                 aaTGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGCTACTTATGAATATGc                                              <  1:542483/251‑1 (MQ=255)                                                                                                                                                                                                              ccAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGCTACTTATGAATATGCTTTGAAAAaaga                                  <  1:8399/250‑1 (MQ=255)                                                                                                                                                                                                                             cAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGaaa                                                                                                         >  1:92889/1‑164 (MQ=255)                                                                                                                                                                                                                             cAGCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGCTACTTATGAATATGCTTTGAAAAAAGAAGAGGAAAGGGCGGAg                  >  1:725817/1‑251 (MQ=255)                                                                                                                                                                                                                               gCTATGACGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGCTACTTa                                                       <  1:131711/212‑1 (MQ=255)                                                                                                                                                                                                                                     aCGAAAGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGCTACTTATGAATATGCTTTGAAAAAAGAAGAGGAAAGGGCGGAGCAACttc           <  1:523825/250‑2 (MQ=255)                                                                                                                                                                                                                                        aaaGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGCTACTTATGAATATGCTTTGAAAAAAGAAGAGGAAAGGGCGGAGCAACTTAGAAt       <  1:859546/251‑1 (MQ=255)                                                                                                                                                                                                                                          aGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATg                                                                                                                                               >  1:167124/1‑113 (MQ=255)                                                                                                                                                                                                                                          aGATAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGCTACTTATGAATATGCTTTGAAAAAAGAAGAGGAAAGGGCGGAGCAACTTAGAATTa     >  1:94523/1‑251 (MQ=255)                                                                                                                                                                                                                                             tAACATTGACTTGGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGCTACTTATGAATATGCTTTGAAAAAAGAAGAGGAAAGGGCGGAGCAACTTAGAATTAAag  <  1:834447/251‑1 (MQ=255)                                                                                                                                                                                                                                             tAACATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGCTACTTATGAATATGCTTTGAAAAAAGAAGAGGAAAGGGCGGAGCAACTTAGAATTAAag  <  1:704356/251‑1 (MQ=255)                                                                                                                                                                                                                                              aaCATTGACTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGCTACTTATGAATATGCTTTGAAAAAAGAAGAGGAAAGGGCGGAGc                 >  1:181972/1‑235 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  TCTTTGATTAAAGATGAGTTAAAAATTTATGAATCAGAAGATGCTTTATTATCAGTAGGTTTATATAAGCCGATGTTTAATTTTGATACTTCGGAAGAGTATAAAGATAAAATTTCAGAACTGAGAGAGAAGCAAAAGGAGTTATTTAAATCAGGCAGAGCTTGTATTTGTTCTACATCTTGGACTGTAGGGAATGGAAAAGATGCCAAGAAGAAAGGCGCAGCTATGACGAAAGATAACATTGATTTAGCTTTAAGGTCGTTTAATGGCGAGTGCGATGCGCTTCTTACGAAAGTAAATGGTCGCAATGTTGATCGTATTAAGAAAAGTATGGAAATGTCTTATGAGCGAATCAACAAGCTAAATGTCCGTAATGTCGTGAAAATACAACCTGCATATTTAAATCTTAAAATTCAAGAGCTGATGGCTACTTATGAATATGCTTTGAAAAAAGAAGAGGAAAGGGCGGAGCAACTTAGAATTAAAG  >  1/2002675‑2003161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑