Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,831,318	T→C	D359D (GAT→GAC)	unknown →	Maltose/maltodextrin transport ATP‑binding protein MalK (EC 3.6.3.19)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,831,318	0	T	C	100.0%	271.2 / NA	80	D359D (GAT→GAC)	unknown	Maltose/maltodextrin transport ATP‑binding protein MalK (EC 3.6.3.19)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/39);  total (41/39)

CCTTTCATTAGGTATACGCCCTGAGCATTTAATTCCGTCTGATGAGGCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGATGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAGCGCAAGTGCGGTGGATTTCCACGGTTACGCCCGTTCCGGTATTGGTTGGACATCGGGCGGT  >  1/1831081‑1831556                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               ccTTTCATTAGGTATACGCCCTGAGCATTTAATTCCGTCTGATGAGGCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGc                                                                                                                                                                                                                                    >  1:694743/1‑250 (MQ=255)    ttCATTAGGTATACGCCCTGAGCATTTAATTCCGTCTGATGAGGCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCaa                                                                                                                                                                                                                                 >  1:888656/1‑250 (MQ=255)           ggTATACGCCCTGAGCATTTAATTCCGTCTGATGAGGCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGta                                                                                                                                                                                                                         >  1:484679/1‑251 (MQ=255)            gTATACGCCCTGAGCATTTAATTCCGTCTGATGAGGCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTGATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGtat                                                                                                                                                                                                                        >  1:327494/1‑251 (MQ=255)            gTATACGCCCTGAGCATTTAATTCCGTCTGATGAGGCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGtt                                                                                                                                                                                                                             <  1:130127/246‑1 (MQ=255)            gTATACGCCCTGAGCATTTAATTCCGTCTGATGAGGCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGtat                                                                                                                                                                                                                        >  1:112229/1‑251 (MQ=255)            gTATACGCCCTGAGCATTTAATTCCGTCTGATGAGGCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAAGGGGAAACGATGGTCATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGtat                                                                                                                                                                                                                        >  1:453748/1‑251 (MQ=255)              ataCGCCCTGAGCATTTAATTCCGTCTGATGAGGCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATaa                                                                                                                                                                                                                      <  1:521564/251‑1 (MQ=255)                  gCCCTGAGCATTTAATTCCGTCTGATGAGGCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGaa                                                                                                                                                                                                                  >  1:529036/1‑251 (MQ=255)                       gAGCATTTAATTCCGTCTGATGAGGCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAgg                                                                                                                                                                                                             >  1:697225/1‑251 (MQ=255)                        aGCATTTAATTCCGTCTGATGAGGCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGGCCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGAACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAgg                                                                                                                                                                                                             <  1:127043/250‑1 (MQ=255)                           aTTTAATTCCGTCTGATGAGGCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGtt                                                                                                                                                                                                         >  1:334065/1‑251 (MQ=255)                                 ttCCGTCTGATGAGGCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAgg                                                                                                                                                                                                   <  1:718649/251‑1 (MQ=255)                                        tgatgaGGCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGAt                                                                                                                                                                                            <  1:231313/251‑1 (MQ=255)                                           tgaGGCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTac                                                                                                                                                                                         <  1:294867/251‑1 (MQ=255)                                              ggCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTacac                                                                                                                                                                                       >  1:474301/1‑250 (MQ=255)                                               gCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTacac                                                                                                                                                                                       <  1:210632/249‑1 (MQ=255)                                               gCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTacac                                                                                                                                                                                       <  1:655044/249‑1 (MQ=255)                                                  gAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGa                                                                                                                                                                                                                   <  1:6863/218‑1 (MQ=255)                                                   aaGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTaga                                                                                                                                                                                 >  1:560358/1‑251 (MQ=255)                                                     gTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAgg                                                                                                                                                                               >  1:89867/1‑251 (MQ=255)                                                     gTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAgg                                                                                                                                                                               >  1:545272/1‑251 (MQ=255)                                                       tACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGcc                                                                                                                                                                             <  1:526300/251‑1 (MQ=255)                                                       tACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGcc                                                                                                                                                                             <  1:420093/251‑1 (MQ=255)                                                           ttGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACgg                                                                                                                                                                         <  1:448809/251‑1 (MQ=255)                                                             gcgcAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTCAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACggg                                                                                                                                                                        >  1:439118/1‑250 (MQ=255)                                                             gcgcAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGtt                                                                                                                                                                                                                             >  1:745529/1‑197 (MQ=255)                                                             gcgcAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGtat                                                                                                                                                                                                                        >  1:8149/1‑202 (MQ=255)                                                                  gcaATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCg                                                                                                                                                                  >  1:780356/1‑251 (MQ=255)                                                                   caATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCgg                                                                                                                                                                 <  1:135812/251‑1 (MQ=255)                                                                   caATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCgg                                                                                                                                                                 <  1:687823/251‑1 (MQ=255)                                                                     aTGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTg                                                                                                                                                               >  1:356499/1‑251 (MQ=255)                                                                       gtgCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTaaa                                                                                                                                                                                                     >  1:792352/1‑211 (MQ=255)                                                                       gtgCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTgg                                                                                                                                                              >  1:282234/1‑250 (MQ=255)                                                                       gtgCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGACACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTagag                                                                                                                                                                                >  1:445686/1‑232 (MQ=255)                                                                        tgCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGc                                                                                                                                                             <  1:196436/250‑1 (MQ=255)                                                                            ggtggtGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACg                                                                                                                                                                                                                               >  1:672388/1‑180 (MQ=255)                                                                            ggtggtGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAgg                                                                                                                                                                                                   <  1:648933/208‑1 (MQ=255)                                                                              tggtggAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAgg                                                                                                                                                       <  1:517415/250‑1 (MQ=255)                                                                                                 cGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAagag                                                                                                                                   <  1:777868/251‑1 (MQ=255)                                                                                                     aCGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTc                                                                                                                                             <  1:534759/237‑1 (MQ=255)                                                                                                               aCCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGaa                                                                                                                     <  1:348798/251‑1 (MQ=255)                                                                                                                ccTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACggg                                                                                                                                                                        >  1:635916/1‑199 (MQ=255)                                                                                                                ccTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGaaa                                                                                                                    <  1:408837/251‑1 (MQ=255)                                                                                                                  ttGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAAt                                                                                                                  >  1:196629/1‑251 (MQ=255)                                                                                                                         cccTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTacac                                                                                                                                                                                       <  1:174077/175‑1 (MQ=255)                                                                                                                         cccTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCaaaaa                                                                                                           >  1:286063/1‑251 (MQ=255)                                                                                                                              aaaTTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAaca                                                                                                        >  1:275668/1‑249 (MQ=255)                                                                                                                                   aaaCAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGtct                                                                                                                             <  1:899586/223‑1 (MQ=255)                                                                                                                                     acaacCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACa                                                                                               <  1:217531/251‑1 (MQ=255)                                                                                                                                            aCCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAgg                                                                                                                                                                                                   <  1:4921/144‑1 (MQ=255)                                                                                                                                            aCCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTac                                                                                                                                                                                         >  1:742262/1‑154 (MQ=255)                                                                                                                                            aCCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCa                                                                                        >  1:171344/1‑251 (MQ=255)                                                                                                                                            aCCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCa                                                                                        >  1:364771/1‑251 (MQ=255)                                                                                                                                             ccTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCAt                                                                                       >  1:164972/1‑251 (MQ=255)                                                                                                                                                               atgatgTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGc                                                                     <  1:300134/251‑1 (MQ=255)                                                                                                                                                                 gatgTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAAc                                                                                                                                               >  1:664449/1‑175 (MQ=255)                                                                                                                                                                 gatgTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGa                                                                                                                      >  1:389065/1‑200 (MQ=255)                                                                                                                                                                  atgTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGcaa                                                                   >  1:87630/1‑250 (MQ=255)                                                                                                                                                                         gtTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAGCGCa                                                           >  1:118662/1‑251 (MQ=255)                                                                                                                                                                           tGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGAc                                                                                                >  1:890232/1‑212 (MQ=255)                                                                                                                                                                             gTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAGCGCAAGTg                                                       >  1:502926/1‑251 (MQ=255)                                                                                                                                                                              tGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAGCGCAAGTg                                                       <  1:358376/250‑1 (MQ=255)                                                                                                                                                                              tGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAGCGCAAGTg                                                       <  1:52506/250‑1 (MQ=255)                                                                                                                                                                                      ggggAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAGCGCAAGTGCGGTGGAt                                               <  1:211357/250‑1 (MQ=255)                                                                                                                                                                                       gggAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAAc                                                                                                                                               >  1:899280/1‑153 (MQ=255)                                                                                                                                                                                          aaaCGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAGCGCAAGTGCGGTGGATTTCCa                                          >  1:873338/1‑251 (MQ=255)                                                                                                                                                                                                    cATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAGCGCAAGTGCGGTGGATTTCCACGGTTACGcc                                <  1:125669/251‑1 (MQ=255)                                                                                                                                                                                                               ttCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAgcg                                                             >  1:654310/1‑211 (MQ=255)                                                                                                                                                                                                               ttCCGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAGCGCAAGTGCGGTGGATTTCCACGGTTACGCCCGTTCCGGTAt                     <  1:292113/251‑1 (MQ=255)                                                                                                                                                                                                                 ccGGAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTacac                                                                                                                                                                                       <  1:599741/87‑1 (MQ=255)                                                                                                                                                                                                                    gAACGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAGCGCAAGTGCGGTGGATTTCCACGGTTACGCCCGTTCCGGTAttggt                 <  1:695303/250‑1 (MQ=255)                                                                                                                                                                                                                     aaCGTTGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAGCGCAAGTGCGGTGGATTTCCACGGTTACGCCCGTTCCGGTAttggtt                <  1:169094/250‑1 (MQ=255)                                                                                                                                                                                                                         ttGCCATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAGCGCAAGTGCGGTGGATTTCCACGGTTACGCCCGTTCCGGTATTGGTTGGAc            <  1:697695/250‑1 (MQ=255)                                                                                                                                                                                                                             cATCTGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAGCGCAAGTGCGGTGGATTTCCACGGTTACGCCCGTTCCGGTATTGGTTGGACATCgg       <  1:159080/251‑1 (MQ=255)                                                                                                                                                                                                                                 tGTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAGCGCAAGTGCGGTGGATTTCCACGGTTACGCCCGTTCCGGTATTGGTTGGACATCGGGCgg   <  1:611600/251‑1 (MQ=255)                                                                                                                                                                                                                                  gTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAGCGCAAGTGCGGTGGATTTCCACGGTTACGCCCGTTCCGGTAttggt                 <  1:586661/236‑1 (MQ=255)                                                                                                                                                                                                                                  gTTTAAAGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAGCGCAAGTGCGGTGGATTTCCACGGTTACGCCCGTTCCGGTATTGGTTGGACATCGGGCGGt  >  1:37369/1‑251 (MQ=255)                                                                                                                                                                                                                                      aaaGAAGACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAGCGCAAGTGCGGTGGATTTCCACGGTTa                                    <  1:615540/213‑1 (MQ=255)                                                                                                                                                                                                                                       aagaagACGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAgg                                                                                                                                                       >  1:687776/1‑97 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               CCTTTCATTAGGTATACGCCCTGAGCATTTAATTCCGTCTGATGAGGCAGAAGTTACGTTGCGCAGCAATGTGCAGGTGGTGGAATTGCTTGGTAACGAAACGCAAATTCACCTTGAAATCCCTGAAATTAAACAACCGACCTTAATTTATCGCCAAAATGATGTGGTGTTGGTGAAGGAGGGGGAAACGATGGACATCGGCATCATTCCGGAACGTTGCCATCTGTTTAAAGAAGATGGCACCGCCTGCCAACGTTTGTATAAAGAAAAAGGCGTTTAAAGGAATTGATTACACCGTAGAGGCCACGGGCTACCGGTGGCTTAAGGTTATTAACTCAATGCAAGAGAGGTCTCTTATGAAAATTCAAAAAACACTTCTGACAATTGCCATTAGCGGCACATTATTTGCAACAAGCGCAAGTGCGGTGGATTTCCACGGTTACGCCCGTTCCGGTATTGGTTGGACATCGGGCGGT  >  1/1831081‑1831556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑