Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,810,410	T→C	L274P (CTT→CCT)	unknown →	Thiamine‑monophosphate kinase (EC 2.7.4.16)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,810,410	0	T	C	100.0%	260.1 / NA	75	L274P (CTT→CCT)	unknown	Thiamine‑monophosphate kinase (EC 2.7.4.16)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/43);  total (32/43)

CCCGACACCAAGGGTGCTGCTGGGATTGGAACTTTCCGTTTCCGGTTTAGCAAATGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACTTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCG  >  1/1810165‑1810645                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            cccGACACCAAGGGTGCTGCTGGGATTGGAACTTTCCGTTTCCGGTTTAGCAAATGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGac                                                                                                                                                                                                                                         <  1:514017/250‑1 (MQ=255) cccGACACCAAGGGTGCTGCTGGGATTGGAACTTTCCGTTTCCGGTTTAGCAAATGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGac                                                                                                                                                                                                                                         <  1:593879/250‑1 (MQ=255) cccGACACCAAGGGTGCTGCTGGGATTGGAACTTTCCGTTTCCGGTTTAGCAAATGCGGCAATCGACATCTCAGACGGGCCGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGac                                                                                                                                                                                                                                         <  1:434974/250‑1 (MQ=255)     acacCAAGGGTGCTGCTGGGATTGGAACTTTCCGTTTCCGGTTTAGCAAATGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGac                                                                                                                                                                                                                                         <  1:78569/246‑1 (MQ=255)     acacCAAGGGTGCTGCTGGGATTGGAACTTTCCGTTTCCGGTTTAGCAAATGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGAcaacaa                                                                                                                                                                                                                                    <  1:768458/251‑1 (MQ=255)     acacCAAGGGTGCTGCTGGGATTGGAACTTTCCGTTTCCGGTTTAGCAAATGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGAcaacaa                                                                                                                                                                                                                                    >  1:51063/1‑251 (MQ=255)        ccAAGGGTGCTGCTGGGATTGGAACTTTCCGTTTCCGGTTTAGCAAATGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAatat                                                                                                                                                                                                                                 <  1:415121/251‑1 (MQ=255)            gggTGCTGCTGGGATTGGAACTTTCCGTTTCCGGTTTAGCAAATGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGt                                                                                                                                                                                                                              >  1:269583/1‑250 (MQ=255)              gTGCTGCTGGGATTGGAACTTTCCGTTTCCGGTTTAGCAAATGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCaa                                                                                                                                                                                                                           >  1:869848/1‑251 (MQ=255)                       ggATTGGAACTTTCCGTTTCCGGTTTAGCAAATGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGaaaa                                                                                                                                                                                                                  >  1:620755/1‑251 (MQ=255)                       ggATTGGAACTTTCCGTTTCCGGTTTAGCAAATGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGaaa                                                                                                                                                                                                                   <  1:346311/250‑1 (MQ=255)                        gATTGGAACTTTCCGTTTCCGGTTTAGCAAATGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGac                                                                                                                                                                                                                                         <  1:70188/227‑1 (MQ=255)                        gATTGGAACTTTCCGTTTCCGGTTTAGCAAATGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGt                                                                                                                                                                                                                              <  1:169625/238‑1 (MQ=255)                        gATTGGAACTTTCCGTTTCCGGTTTAGCAAATGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGaaaaa                                                                                                                                                                                                                 >  1:133041/1‑251 (MQ=255)                        gATTGGAACTTTCCGTTTCCGGTTTAGCAAATGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGaaaaa                                                                                                                                                                                                                 >  1:716517/1‑251 (MQ=255)                                          ccgGTTTAGCAAATGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGc                                                                                                                                                                                               <  1:621638/251‑1 (MQ=255)                                                      aTGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATAcc                                                                                                                                                                                   <  1:346499/251‑1 (MQ=255)                                                      aTGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATAcc                                                                                                                                                                                   <  1:128199/251‑1 (MQ=255)                                                         cGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTg                                                                                                                                                                                 <  1:772020/250‑1 (MQ=255)                                                            cAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTTCCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTAt                                                                                                                                                                              <  1:488179/250‑1 (MQ=255)                                                            cAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTAt                                                                                                                                                                              <  1:9021/250‑1 (MQ=255)                                                            cAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTAt                                                                                                                                                                              <  1:381020/250‑1 (MQ=255)                                                            cAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTAt                                                                                                                                                                              <  1:394848/250‑1 (MQ=255)                                                            cAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTAt                                                                                                                                                                              <  1:561072/250‑1 (MQ=255)                                                            cAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTAt                                                                                                                                                                              <  1:624338/250‑1 (MQ=255)                                                                  aCATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCggggaa                                                                                                                                                                       <  1:865134/251‑4 (MQ=255)                                                                     tctcAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAAtt                                                                                                                                                                    >  1:584138/1‑251 (MQ=255)                                                                        cAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAAtt                                                                                                                                                                    >  1:430345/1‑248 (MQ=255)                                                                          gACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTAt                                                                                                                                                                              <  1:536659/236‑1 (MQ=255)                                                                              ggCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGgcg                                                                                                                                                                                                        <  1:314518/206‑1 (MQ=255)                                                                              ggCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTAt                                                                                                                                                                              >  1:196385/1‑232 (MQ=255)                                                                                         gATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACg                                                                                                                                                >  1:570666/1‑251 (MQ=255)                                                                                               gggCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTg                                                                                                                                                                                 <  1:188396/212‑1 (MQ=255)                                                                                                     atTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATAcc                                                                                                                                                                                   >  1:4192/1‑204 (MQ=255)                                                                                                      ttttAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTAt                                                                                                                                                                              >  1:60116/1‑208 (MQ=255)                                                                                                      ttttAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGt                                                                                                                                        >  1:850580/1‑246 (MQ=255)                                                                                                                cAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTAt                                                                                                                                                                              >  1:138956/1‑198 (MQ=255)                                                                                                                cAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTAt                                                                                                                                                                              <  1:630265/198‑1 (MQ=255)                                                                                                                cAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACggg                                                                                                                          >  1:328376/1‑250 (MQ=255)                                                                                                                      aTGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATAcc                                                                                                                                                                                   <  1:636009/187‑1 (MQ=255)                                                                                                                      aTGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTAt                                                                                                                                                                              >  1:768558/1‑192 (MQ=255)                                                                                                                       tGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGt                                                                                                                  <  1:361322/251‑1 (MQ=255)                                                                                                                        gCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTAt                                                                                                                                                                              <  1:389428/190‑1 (MQ=255)                                                                                                                                      gAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAat                                                                                                                                                                                                                                   <  1:253973/123‑1 (MQ=255)                                                                                                                                      gAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAGTATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGc                                                                                                    >  1:373413/1‑250 (MQ=255)                                                                                                                                      gAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGc                                                                                                    >  1:143745/1‑250 (MQ=255)                                                                                                                                             aTAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACgg                                                                                             <  1:319748/250‑1 (MQ=255)                                                                                                                                             aTAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACgg                                                                                             <  1:624038/250‑1 (MQ=255)                                                                                                                                                aaTTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGtttt                                                                                         >  1:532939/1‑251 (MQ=255)                                                                                                                                                aaTTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGtttt                                                                                         >  1:822710/1‑251 (MQ=255)                                                                                                                                                    gCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGt                                                                                            >  1:242867/1‑244 (MQ=255)                                                                                                                                                     ccccTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATc                                                                                     <  1:133084/250‑1 (MQ=255)                                                                                                                                                     ccccTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATc                                                                                     <  1:295922/250‑1 (MQ=255)                                                                                                                                                     ccccTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCCGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATc                                                                                     <  1:340660/250‑1 (MQ=255)                                                                                                                                                                 gAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGt                                                                        >  1:89542/1‑251 (MQ=255)                                                                                                                                                                 gAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGt                                                                        >  1:345655/1‑251 (MQ=255)                                                                                                                                                                              aaGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGTGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACg                                                           <  1:835463/251‑1 (MQ=255)                                                                                                                                                                              aaGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACg                                                           <  1:246833/251‑1 (MQ=255)                                                                                                                                                                                       tGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTacaa                                                  <  1:405256/251‑1 (MQ=255)                                                                                                                                                                                         gCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTacaac                                                 <  1:86550/250‑1 (MQ=255)                                                                                                                                                                                         gCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTacaac                                                 <  1:731605/250‑1 (MQ=255)                                                                                                                                                                                             aaGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACg                                                           >  1:333875/1‑236 (MQ=255)                                                                                                                                                                                                     cAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACg                                    >  1:206319/1‑251 (MQ=255)                                                                                                                                                                                                                gTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTacaac                                                 <  1:559096/227‑1 (MQ=255)                                                                                                                                                                                                                             gACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATcc                  <  1:739772/245‑1 (MQ=255)                                                                                                                                                                                                                              aCTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTc             <  1:556538/249‑1 (MQ=255)                                                                                                                                                                                                                              aCTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCAt           <  1:350670/251‑1 (MQ=255)                                                                                                                                                                                                                              aCTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCAt           <  1:494236/251‑1 (MQ=255)                                                                                                                                                                                                                               cTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTg                                                                                                                                                                                 >  1:40722/1‑84 (MQ=255)                                                                                                                                                                                                                                      cTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTcg    >  1:476720/1‑250 (MQ=255)                                                                                                                                                                                                                                      cTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTcg    >  1:333770/1‑250 (MQ=255)                                                                                                                                                                                                                                        ttGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTcgcg  >  1:747452/1‑250 (MQ=255)                                                                                                                                                                                                                                          gTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAAt                                                                            >  1:643122/1‑174 (MQ=255)                                                                                                                                                                                                                                          gTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTac                                                    >  1:465638/1‑198 (MQ=255)                                                                                                                                                                                                                                                ccGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTacaa                                                  >  1:128355/1‑194 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            CCCGACACCAAGGGTGCTGCTGGGATTGGAACTTTCCGTTTCCGGTTTAGCAAATGCGGCAATCGACATCTCAGACGGGCTGGTTGCCGATTTAGGGCATATTTTAGAACGCAGCCAATGCGGTGCGATGATTGAATTGGATAAATTGCCCCTTTCCGCCGAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACTTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCG  >  1/1810165‑1810645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑