Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,775,066 T→G M196L (ATG→CTG)  unknown ← tRNA dimethylallyltransferase (EC 2.5.1.75)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,775,0660TG100.0% 238.2 / NA 69M196L (ATG→CTG) unknowntRNA dimethylallyltransferase (EC 2.5.1.75)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (34/35);  total (34/35)

GCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCATAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGA  >  1/1774822‑1775311
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     
gCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAAtct                                                                                                                                                                                                                                                  >  1:902089/1‑250 (MQ=255)
   aaTTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATa                                                                                                                                                                                                                                              >  1:169400/1‑251 (MQ=255)
        gCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGc                                                                                                                                                                                                                                          <  1:856113/250‑1 (MQ=255)
        gCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGc                                                                                                                                                                                                                                          <  1:697904/250‑1 (MQ=255)
        gCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGc                                                                                                                                                                                                                                          <  1:711361/250‑1 (MQ=255)
                      tGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCtttt                                                                                                                                                                                                                           <  1:5998/251‑1 (MQ=255)
                                  ccATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAAt                                                                                                                                                                                                               >  1:791054/1‑251 (MQ=255)
                                           catGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAg                                                                                                                                                                                                      <  1:863947/251‑1 (MQ=255)
                                             tGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGt                                                                                                                                                                                                    >  1:87256/1‑251 (MQ=255)
                                             tGGCTATAGTCACCACGCAAATATTCCCACATTTGACGAT‑CCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGtt                                                                                                                                                                                                   >  1:42714/1‑251 (MQ=255)
                                                cTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATAGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGtttt                                                                                                                                                                                                 >  1:808760/1‑251 (MQ=255)
                                                                          caTTTGACGATTCCCCACACAGCGAATTGATGGCATATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGTCCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATTCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGTCGAATTGCAGAATCTCATAGGGCAGCGCTTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAac                                                                                                                                                                                 <  1:223358/241‑1 (MQ=255)
                                                                          caTTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAg                                                                                                                                                                                                                                             <  1:544933/181‑1 (MQ=255)
                                                                          caTTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTg                                                                                                                                                                       >  1:246172/1‑251 (MQ=255)
                                                                          caTTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTg                                                                                                                                                                       >  1:59159/1‑251 (MQ=255)
                                                                            tttGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAG‑TTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTgcgc                                                                                                                                                                    <  1:176895/251‑1 (MQ=255)
                                                                                aCGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAgg                                                                                                                                                                                                     <  1:883679/215‑1 (MQ=255)
                                                                                   aTACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGc                                                                                                                                                                                               >  1:146704/1‑218 (MQ=255)
                                                                                   aTACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAac                                                                                                                                                                                 >  1:681012/1‑232 (MQ=255)
                                                                                     aCCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGc                                                                                                                                                                                                                                          >  1:693253/1‑173 (MQ=255)
                                                                                     aCCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAac                                                                                                                                                                                 >  1:753923/1‑230 (MQ=255)
                                                                                     aCCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAAt                                                                                                                                                             >  1:474869/1‑250 (MQ=255)
                                                                                     aCCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATc                                                                                                                                                            <  1:611164/251‑1 (MQ=255)
                                                                                      ccccACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATcc                                                                                                                                                           <  1:792532/251‑1 (MQ=255)
                                                                                      ccccACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATc                                                                                                                                                            >  1:103268/1‑250 (MQ=255)
                                                                                       cccACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCg                                                                                                                                                          <  1:70520/251‑1 (MQ=255)
                                                                                                  aaTGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCAt                                                                                                                                               <  1:352589/251‑1 (MQ=255)
                                                                                                               ttCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTg                                                                                                                                                                       >  1:840359/1‑214 (MQ=255)
                                                                                                                     tGCAAATCAGCGCGTCGGTGCAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTgcg                                                                                                                             >  1:274646/1‑250 (MQ=255)
                                                                                                                               cgcgTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATg                                                                                                                     <  1:182218/248‑1 (MQ=255)
                                                                                                                                 cgTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTg                                                                                                                                                                       <  1:217331/196‑1 (MQ=255)
                                                                                                                                   tCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGa                                                                                                              >  1:355692/1‑251 (MQ=255)
                                                                                                                                      gTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGAtt                                                                                                            <  1:727069/250‑1 (MQ=255)
                                                                                                                                                     ctctTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACGCTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGAt                                                                                            <  1:778308/251‑1 (MQ=255)
                                                                                                                                                     ctctTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGAt                                                                                            <  1:897735/251‑1 (MQ=255)
                                                                                                                                                       ctTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGt                                                                                          >  1:599541/1‑251 (MQ=255)
                                                                                                                                                       ctTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGt                                                                                          >  1:188537/1‑251 (MQ=255)
                                                                                                                                                       ctTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGt                                                                                          <  1:715262/251‑1 (MQ=255)
                                                                                                                                                        ttgttgAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTa                                                                                         <  1:269293/251‑1 (MQ=255)
                                                                                                                                                             gAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATgc                                                                                     >  1:506121/1‑250 (MQ=255)
                                                                                                                                                                      gTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTg                                                                                                                                                                       <  1:566617/159‑1 (MQ=255)
                                                                                                                                                                      gTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGt                                                                                          >  1:61276/1‑236 (MQ=255)
                                                                                                                                                                           aTCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACg                                                                      <  1:90624/251‑1 (MQ=255)
                                                                                                                                                                             cATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGtt                                                                    >  1:808156/1‑251 (MQ=255)
                                                                                                                                                                              aTTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCgaga                                                                                                                       <  1:186027/199‑1 (MQ=255)
                                                                                                                                                                              aTTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTc                                                                   <  1:439846/251‑1 (MQ=255)
                                                                                                                                                                                              gCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATa                                                                        <  1:194819/230‑1 (MQ=255)
                                                                                                                                                                                              gCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAAtt                                                   >  1:609157/1‑251 (MQ=255)
                                                                                                                                                                                              gCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAAtt                                                   >  1:787536/1‑251 (MQ=255)
                                                                                                                                                                                               cTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAAttt                                                  <  1:892635/251‑1 (MQ=255)
                                                                                                                                                                                                tCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAAttt                                                  >  1:588064/1‑250 (MQ=255)
                                                                                                                                                                                                     gCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGc                                                                <  1:431386/231‑1 (MQ=255)
                                                                                                                                                                                                     gCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGc                                             >  1:861223/1‑250 (MQ=255)
                                                                                                                                                                                                     gCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGc                                             >  1:286339/1‑250 (MQ=255)
                                                                                                                                                                                                              cAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGt                                                                                                                                                                 >  1:262881/1‑125 (MQ=255)
                                                                                                                                                                                                               aaCAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTc                                  <  1:323557/251‑1 (MQ=255)
                                                                                                                                                                                                                   gATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACg                                                                      <  1:126204/211‑1 (MQ=255)
                                                                                                                                                                                                                   gATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATcg                              >  1:145214/1‑251 (MQ=255)
                                                                                                                                                                                                                   gATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTCTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATcg                              >  1:895675/1‑251 (MQ=255)
                                                                                                                                                                                                                       tctctGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATgc                                                                                     <  1:407442/192‑1 (MQ=255)
                                                                                                                                                                                                                        ctctGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTc                                                                                                      <  1:799722/174‑1 (MQ=255)
                                                                                                                                                                                                                               ccGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAAt                  >  1:857563/1‑251 (MQ=255)
                                                                                                                                                                                                                                  gTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATgg                >  1:195680/1‑250 (MQ=255)
                                                                                                                                                                                                                                  gTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATgg                <  1:877740/250‑1 (MQ=255)
                                                                                                                                                                                                                                  gTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATgg                >  1:538148/1‑250 (MQ=255)
                                                                                                                                                                                                                                  gTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACCTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACg                                                                      <  1:660440/196‑1 (MQ=255)
                                                                                                                                                                                                                                          gCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGc        <  1:348426/250‑1 (MQ=255)
                                                                                                                                                                                                                                           cGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGcc       <  1:332588/250‑1 (MQ=255)
                                                                                                                                                                                                                                               ttGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGa  >  1:642812/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     
GCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCATAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGA  >  1/1774822‑1775311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑