Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,714,984	C→T	K188K (AAG→AAA)	unknown ←	DNA topoisomerase III (EC 5.99.1.2) in PFGI‑1‑like cluster

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,714,984	0	C	T	100.0%	135.5 / NA	41	K188K (AAG→AAA)	unknown	DNA topoisomerase III (EC 5.99.1.2) in PFGI‑1‑like cluster
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/20);  total (21/20)

CGCCGGCTTGTTGCACTTGTTGATTCACTGCTTGTATCGTTTGTTGGGATAAACAGAGTCCGTCCTCATCACAGTGTTGCTCAGGGATAACATATTGAGCAGTAAAATTCTGTTGCCCAGAAGAGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGCTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAATAAGGCTAAGGCTTTACGAATACTGACATCATCCAATGCCGATAACCAGAGGCGCTGGATCCCGCCTTTAAATCCGGCTAACTCTAATAATTCCCGGGCGATG  >  1/1714749‑1715218                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           cGCCGGCTTGTTGCACTTGTTGATTCACTGCTTGTATCGTTTGTTGGGATAAACAGAGTCCGTCCTAATCACAGTGTTGCTCAGGGATAACATATTGAGCAGTAAAATTCTGTTGCCCAGAAGAGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCt                                                                                                                                                                                                                             <  1:158699/251‑1 (MQ=255)                                        gttgttgGGATAAACAGAGTCCGTCCTAATCACAGTGTTGCTCAGGGATAACATATTGAGCAGTAAAATTCTGTTGCCCAGAAGAGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAAtt                                                                                                                                                                                       <  1:3052/249‑1 (MQ=255)                                                    aaCAGAGTCCGTCCTAATCACAGTGTTGCTCAGGGATAACATATTGAGCAGTAAAATTCTGTTGCCCAGAAGAGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAg                                                                                                                                                                           >  1:292444/1‑250 (MQ=255)                                                              gtccTAATCACAGTGTTGCTCAGGGATAACATATTGAGCAGTAAAATTCTGTTGCCCAGAAGAGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGcc                                                                                                                                                                 >  1:367924/1‑250 (MQ=255)                                                                              tGCTCAGGGATAACATATTGAGCAGTAAAATTCTGTTGCCCAGAAGAGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATcc                                                                                                                                                <  1:598227/251‑1 (MQ=255)                                                                                    gggATAACATATTGAGCAGTAAAATTCTGTTGCCCAGAAGAGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCata                                                                                                                                          >  1:591169/1‑251 (MQ=255)                                                                                     ggATAACATATTGAGCAGTAAAATTCTGTTGCCCAGAAGAGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCa                                                                                                                                            <  1:625441/248‑1 (MQ=255)                                                                                     ggATAACATATTGAGCAGTAAAATTCTGTTGCCCAGAAGAGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCa                                                                                                                                            <  1:325195/248‑1 (MQ=255)                                                                                       aTAACATATTGAGCAGTAAAATTCTGTTGCCCAGAAGAGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCataata                                                                                                                                       >  1:550005/1‑251 (MQ=255)                                                                                         aaCATATTGAGCAGTAAAATTCTGTTGCCCAGAAGAGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAAtata                                                                                                                                     >  1:103529/1‑251 (MQ=255)                                                                                            atatTGAGCAGTAAAATTCTGTTGCCCAGAAGAGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAAtt                                                                                                                                                                                       <  1:170796/198‑1 (MQ=255)                                                                                            atatTGAGCAGTAAAATTCTGTTGCCCAGAAGAGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCg                                                                                                                                                                   >  1:472259/1‑218 (MQ=255)                                                                                             tatTGAGCAGTAAAATTCTGTTGCCCAGAAGAGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACgg                                                                                                                                 <  1:351814/251‑1 (MQ=255)                                                                                                    cAGTAAAATTCTGTTGCCCAGAAGAGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGt                                                                                                                           <  1:411542/250‑1 (MQ=255)                                                                                                           aTTCTGTTGCCCAGAAGAGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACgg                                                                                                                                 >  1:342737/1‑237 (MQ=255)                                                                                                                  tGCCCAGAAGAGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGttt                                                                                                            <  1:573147/251‑1 (MQ=255)                                                                                                                        gaagaGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAatat                                                                                                                                      >  1:49185/1‑219 (MQ=255)                                                                                                                        gaagaGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAAtaa                                                                                                      >  1:642635/1‑251 (MQ=255)                                                                                                                        gaagaGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAAtaa                                                                                                      >  1:338075/1‑251 (MQ=255)                                                                                                                        gaagaGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAAtaa                                                                                                      >  1:282008/1‑251 (MQ=255)                                                                                                                        gaagaGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAAtaa                                                                                                      >  1:15682/1‑251 (MQ=255)                                                                                                                            agagGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAAtaaggc                                                                                                   <  1:153575/250‑1 (MQ=255)                                                                                                                                  gTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAAtaaggctaagg                                                                                              >  1:757217/1‑249 (MQ=255)                                                                                                                                   tGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAAtaaggctaaggc                                                                                             <  1:355638/249‑1 (MQ=255)                                                                                                                                   tGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAAtaaggctaaggc                                                                                             <  1:259943/249‑1 (MQ=255)                                                                                                                                   tGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAAtaaggctaaggc                                                                                             <  1:222920/249‑1 (MQ=255)                                                                                                                                        aTAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAATAAGGCTAAGGCTTTACg                                                                                       <  1:809813/250‑1 (MQ=255)                                                                                                                                                aaaTGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAGATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAatat                                                                                                                                      <  1:767713/195‑1 (MQ=255)                                                                                                                                                  aTGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAATAAGGCTAAGGCTTTACGAATACTGAcat                                                                            <  1:731224/251‑1 (MQ=255)                                                                                                                                                                              tctcTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAATAAGGCTAAGGCTTTACGAATACTGACATCATCCAATGCCGATAACCAGAGgcg                                                   >  1:412801/1‑248 (MQ=255)                                                                                                                                                                                              cAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAATAAGGCTAAGGCTTTACGAATACTGACATCATCCAATGCCGATAACCAGAGGCGCTGGATCCCGCCTTTAAAt                                <  1:58750/251‑1 (MQ=255)                                                                                                                                                                                              cAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAATAAGGCTAAGGCTTTACGAATACTGACATCATCCAATGCCGATAACCAGAGGCGCTGGATCCCGCCTTTAAAt                                >  1:169862/1‑251 (MQ=255)                                                                                                                                                                                                     aGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAatat                                                                                                                                      <  1:196142/142‑1 (MQ=255)                                                                                                                                                                                                          gggTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATg                                                                                                                     >  1:895234/1‑154 (MQ=255)                                                                                                                                                                                                          gggTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAATAAGGCTAAGGCTTTACGAATACTGACATCATCCAATGCCGATAACCAGAGGCGCTGGATCCCGCCTTTAAATCCGGCTAActc                     >  1:142832/1‑250 (MQ=255)                                                                                                                                                                                                          gggTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAATAAGGCTAAGGCTTTACGAATACTGACATCATCCAATGCCGATAACCAGAGGCGCTGGATCCCGCCTTTAAATCCGGCTAAct                      >  1:29860/1‑249 (MQ=255)                                                                                                                                                                                                           ggTAGGACTTTGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAAt                                                                                                        >  1:324237/1‑166 (MQ=255)                                                                                                                                                                                                                gACTTTGCCCCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAATAAGGCTAAGGCTTTACGAATACTGACATCATCCAATGCCGATAACCAGAGGCGCTGGATCCCGCCTTTAAATCCGGCTAACTCtaataa               <  1:263287/250‑1 (MQ=255)                                                                                                                                                                                                                    ttGCACCCGTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAATAAGGCTAAGGCTTTACGAATACTGACATCATCCAATGCCGATAACCAGAGGCGCTGGATCCCGCCTTTAAATCCGGCTAACTCTAATAATTcc           >  1:766176/1‑250 (MQ=255)                                                                                                                                                                                                                            gTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGcc                                                                                                                                                                         <  1:750727/84‑1 (MQ=255)                                                                                                                                                                                                                            gTCCCACTGAAAGCGGTTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAATAAGGCTAAGGCTTTACGAATACTGACATCATCCAATGCCGATAACCAGAGGCGCTGGATCCCGCCTTTAAATCCGGCTAACTCTAATAATTCCCGGGCGATg  >  1:548605/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           CGCCGGCTTGTTGCACTTGTTGATTCACTGCTTGTATCGTTTGTTGGGATAAACAGAGTCCGTCCTCATCACAGTGTTGCTCAGGGATAACATATTGAGCAGTAAAATTCTGTTGCCCAGAAGAGAGGAGTGCGGATAAGGTAAAATGATTTTTGGGAACAAAGTTTTGAATTTCTCTATCTCGATTGACAACTAAAGCAAGGGTAGGACTTTGCACCCGTCCCACTGAAAGCGGCTTGCCTTGGTAACCTTGTTGTTGAGCCAGCAAGGTAAATAACCGGGAAAAATTCATGCCAATGAGCCAATCCGCCCGACTACGCCCTAATCCGGCATAATATAACGGTAAGGTTTCATGATTAGGTTTCAATAAGGCTAAGGCTTTACGAATACTGACATCATCCAATGCCGATAACCAGAGGCGCTGGATCCCGCCTTTAAATCCGGCTAACTCTAATAATTCCCGGGCGATG  >  1/1714749‑1715218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑