Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,703,451	A→C	*739E (TAA→GAA)	unknown ←	Type IV secretory pathway, VirD4 components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,703,451	0	A	C	100.0%	236.2 / NA	70	*739E (TAA→GAA)	unknown	Type IV secretory pathway, VirD4 components
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (36/34);  total (36/34)

TTCTTCGGTCACCGGAATATCATTGGCAGTCCATAGTGTGAGTTGCATCGCATATTTTTGTGCAAATTCATGGACGCTGTATTGGTGTTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTAATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATAATTTTTACGCATATATGCGGTCAGTTCTTGCAACGATTGAGGCATGATAT  >  1/1703206‑1703693                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   ttcttcGGTCACCGGAATATCATTGGCAGTCCATAGTGTGAGTTGCATCGCATATTTTTGTGCAAATTCATGGACGCTGTATTGGTGTTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCgc                                                                                                                                                                                                                                               <  1:899070/251‑2 (MQ=255)                   atCATTGGCAGTCCATAGTGTGAGTTGCATCGCATATTTTTGTGCAAATTCATGGACGCTGTATTGGTGTTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACAt                                                                                                                                                                                                                             >  1:390213/1‑251 (MQ=255)                             gTCCATAGTGTGAGTTGCATCGCATATTTTTGTGCAAATTCATGGACGCTGTATTGGTGTTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGt                                                                                                                                                                                                                    >  1:203418/1‑250 (MQ=255)                              tCCATAGTGTGAGTTGCATCGCATATTTTTGTGCAAATTCATGGACGCTGTATTGGTGTTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAACTCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGt                                                                                                                                                                                                                    >  1:430177/1‑249 (MQ=255)                              tCCATAGTGTGAGTTGCATCGCATATTTTTGTGCAAATTCATGGACGCTGTATTGGTGTTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGt                                                                                                                                                                                                                    >  1:502098/1‑249 (MQ=255)                                           ttGCATCGCATATTTTTGTGCAAATTCATGGACGCTGTATTGGTGTTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCcac                                                                                                                                                                                                       <  1:880703/249‑1 (MQ=255)                                                   cATATTTTTGTGCAAATTCATGGACGCTGTATTGGTGTTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCa                                                                                                                                                                                             <  1:768503/251‑1 (MQ=255)                                                       tttttGTGCAAATTCATGGACGCTGTATTGGTGTTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGt                                                                                                                                                                                         <  1:722083/251‑1 (MQ=255)                                                               cgaatacATGGACGCTGTATTGGTGTTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCatat                                                                                                                                                                                 <  1:236729/245‑1 (MQ=255)                                                               cAAATTCATGGACGCTGTATTGGTGTTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCatat                                                                                                                                                                                 <  1:249716/251‑1 (MQ=255)                                                                   ttCATGGACGCTGTATTGGTGTTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCa                                                                                                                                                                             >  1:100331/1‑251 (MQ=255)                                                                             cTGTATTGGTGTTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAg                                                                                                                                                                            <  1:539807/242‑1 (MQ=255)                                                                               gTATTGGTGTTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCg                                                                                                                                                                                                                                                <  1:106196/172‑1 (MQ=255)                                                                               gTATTGGTGTTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGa                                                                                                                                                                 >  1:85806/1‑251 (MQ=255)                                                                               gTATTGGTGTTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGa                                                                                                                                                                 >  1:679443/1‑251 (MQ=255)                                                                                     gtgtTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCg                                                                                                                                                                                                                                                >  1:492149/1‑166 (MQ=255)                                                                                       gtTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAg                                                                                                                                                                            >  1:188322/1‑232 (MQ=255)                                                                                        ttCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAg                                                                                                                                                        <  1:233118/251‑1 (MQ=255)                                                                                            cTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAgttgt                                                                                                                                                    >  1:823653/1‑251 (MQ=255)                                                                                            cTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAgttgt                                                                                                                                                    >  1:142866/1‑251 (MQ=255)                                                                                              ccAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGttt                                                                                                                                                  <  1:222614/251‑1 (MQ=255)                                                                                                   cATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGc                                                                                                                                                                              >  1:40235/1‑218 (MQ=255)                                                                                                          gTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGc                                                                                                                                        <  1:857811/249‑1 (MQ=255)                                                                                                              ggAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGa                                                                                                                                  >  1:838279/1‑251 (MQ=255)                                                                                                               gAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAg                                                                                                                                   <  1:363970/249‑1 (MQ=255)                                                                                                               gAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAg                                                                                                                                   <  1:194506/249‑1 (MQ=255)                                                                                                                   aaaaTAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATatc                                                                                                                                                                                                                                        >  1:670762/1‑144 (MQ=255)                                                                                                                    aaaTAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGaaa                                                                                                                            >  1:369603/1‑251 (MQ=255)                                                                                                                         gTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAAt                                                                                                                                                                                           >  1:191574/1‑183 (MQ=255)                                                                                                                            cATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGc                                                                                                                                                                              >  1:541821/1‑193 (MQ=255)                                                                                                                            cATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCgg                                                                                                                    >  1:288031/1‑251 (MQ=255)                                                                                                                                       ccTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCAtgt                                                                                                         <  1:84049/251‑1 (MQ=255)                                                                                                                                          gATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCAtgt                                                                                                         <  1:174924/248‑1 (MQ=255)                                                                                                                                            ttttttAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCaa                                                                                                                                                                                            >  1:36504/1‑163 (MQ=255)                                                                                                                                            ttttttAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACgg                                                                                                    <  1:784658/251‑1 (MQ=255)                                                                                                                                                       acaGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGaaa                                                                                                                            >  1:417922/1‑216 (MQ=255)                                                                                                                                                       acaGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCgg                                                                                         >  1:681228/1‑251 (MQ=255)                                                                                                                                                               cTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCa                                                                                                            <  1:143232/224‑1 (MQ=255)                                                                                                                                                                           ggTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCatat                                                                                                                                                                                 <  1:685171/143‑1 (MQ=255)                                                                                                                                                                              tAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGttt                                                                  <  1:550794/251‑1 (MQ=255)                                                                                                                                                                                   tCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTAc                                                             <  1:251978/251‑1 (MQ=255)                                                                                                                                                                                       cTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGTAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGata                                                         <  1:433304/251‑1 (MQ=255)                                                                                                                                                                                        ttGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGatat                                                        <  1:465771/251‑1 (MQ=255)                                                                                                                                                                                           cATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGAt                                                     <  1:366934/251‑1 (MQ=255)                                                                                                                                                                                             tatCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATaa                                                   <  1:742588/251‑1 (MQ=255)                                                                                                                                                                                                      aaTCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATAATTTTTACGc                                          <  1:79913/251‑1 (MQ=255)                                                                                                                                                                                                      aaTCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATAATTTTTACGc                                          <  1:738501/251‑1 (MQ=255)                                                                                                                                                                                                       aTCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATAATTTTTACGCa                                         >  1:902081/1‑251 (MQ=255)                                                                                                                                                                                                       aTCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATAATTTTTACGCa                                         >  1:266133/1‑251 (MQ=255)                                                                                                                                                                                                              aaaTTGTAATGACACATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATAATTTTTACGCATATATGc                                  <  1:363225/251‑1 (MQ=255)                                                                                                                                                                                                                         acacATTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATAATTTTTACGCATATATGCGGTCAGTTCtt                       >  1:400633/1‑251 (MQ=255)                                                                                                                                                                                                                          cacaTTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAAc                                                                                                                           <  1:569365/150‑1 (MQ=255)                                                                                                                                                                                                                          cacaTTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATAATTTTTACGCATATATGCGGTCAGTTCTTg                      >  1:186313/1‑251 (MQ=255)                                                                                                                                                                                                                            caTTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCat                                                                                                                                                                                   <  1:730433/92‑1 (MQ=255)                                                                                                                                                                                                                            caTTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGAc                                                                                                                                                                >  1:492265/1‑111 (MQ=255)                                                                                                                                                                                                                            caTTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCccacc                                                                       <  1:688731/200‑1 (MQ=255)                                                                                                                                                                                                                            caTTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATaa                                                   <  1:787628/220‑1 (MQ=255)                                                                                                                                                                                                                            caTTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACAGCATGTATTCGGAAAATGCTCCTTCccacc                                                                       >  1:454591/1‑200 (MQ=255)                                                                                                                                                                                                                             aTTTAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATAATTTTTACGCATATATGCGGTCAGTTCTTGCaa                   >  1:513139/1‑251 (MQ=255)                                                                                                                                                                                                                              tttAGTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTAt                                                                                             >  1:606739/1‑176 (MQ=255)                                                                                                                                                                                                                                  gTCTTCTCCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTCAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTAc                                                             <  1:885341/204‑1 (MQ=255)                                                                                                                                                                                                                                      tctcCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCa                                                                                                                                                                                             >  1:293150/1‑72 (MQ=255)                                                                                                                                                                                                                                      tctcCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATaa                                                   <  1:604815/210‑1 (MQ=255)                                                                                                                                                                                                                                      tctcCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATAATTTTTACGCATATATGCGGTCAGTTCTTGCAACGATTGAgg          >  1:707239/1‑251 (MQ=255)                                                                                                                                                                                                                                      tctcCATCATCACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATAATTTTTACGCATATATGCGGTCAGTTCTTGAAACGATTGAgg          >  1:790957/1‑251 (MQ=255)                                                                                                                                                                                                                                           atcatcACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATAATTTTTACGCATATATGCGGTCAGTTCTTGCAACGATTGAGGc         >  1:867290/1‑247 (MQ=255)                                                                                                                                                                                                                                           atcatcACCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATAATTTTTACGCATATATGCGGTCAGTTCTTGCAACGATTGAGGCATGa     >  1:522491/1‑251 (MQ=255)                                                                                                                                                                                                                                            tcatcaCCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATAATTTTTACGCATATATGCGGTCAGTTCTTGCAACGATTGAGGCATGat    <  1:371329/251‑1 (MQ=255)                                                                                                                                                                                                                                               tcaCCTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATAATTTTTACGCATATATGCGGTCAGTTCTTGCAACGATTGAGGCATGatat  >  1:442935/1‑250 (MQ=255)                                                                                                                                                                                                                                                  ccTTCATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATaa                                                   >  1:683777/1‑198 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   TTCTTCGGTCACCGGAATATCATTGGCAGTCCATAGTGTGAGTTGCATCGCATATTTTTGTGCAAATTCATGGACGCTGTATTGGTGTTCACTCCAATCATTTAGGTAAGGAATAAAATAGTCCATCAATTCATCCTGATTTTTTAACCCACAGGAGACTGATATTGGATGGTTAAAATCAACTTGCATATCCGGTAAATCTAATAAATTGTAATGACACATTTAGTCTTCTCCATCATCACCTTAATCGTCTATATCATCGGCAACATTTTGTTCGTCTTCCTCTTCCACAAGCATATCAATGTCACCATATTGCAGCTCGTCATTGACAACCGCAGTTGTTTTATTTAATGCCATAGATTGAAACGCCTCGGCAATATCATGTGACGGCATGTATTCGGAAAATGCTCCTTCCCACCAGTTTGGTACGATATGATAATTTTTACGCATATATGCGGTCAGTTCTTGCAACGATTGAGGCATGATAT  >  1/1703206‑1703693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑