Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,694,421	A→C	S79A (TCT→GCT)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,694,421	0	A	C	100.0%	245.0 / NA	71	S79A (TCT→GCT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (34/37);  total (34/37)

GTGTGATAATAAAGCCGAGTATAAATTTTAAACGACGACGTGAAGGCTTCGTGTTATTCGGTGTGTTGTTCATTATTTGCCTCACTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGAAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATGACTGGACTCTTGGACCTATCTCAAATAAATACGCATAATTCATCAGATTAGGC  >  1/1694177‑1694665                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     gtgtGATAATAAAGCCGAGTATAAATTTTAAACGACGACGTGAAGGCTTCGTGTTATTCGGTGTGTTGTTCATTATTTGCCTCACTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACg                                                                                                                                                                                                                                                >  1:294989/1‑251 (MQ=255)                                       cgTGAAGGCTTCGTGTTATTCGGTGTGTTGTTCATTATTTGCCTCACTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAAt                                                                                                                                                                                                          <  1:318705/251‑1 (MQ=255)                                           aaGGCTTCGTGTTATTCGGTGTGTTGTTCATTATTTGCCTCACTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCAcc                                                                                                                                                                                                      <  1:606184/251‑1 (MQ=255)                                                  cGTGTTATTCGGTGTGTTGTTCATTATTTGCCTCACTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGata                                                                                                                                                                                               >  1:639647/1‑251 (MQ=255)                                                       tATTCGGTGTGTTGTTCATTATTTGCCTCACTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCAc                                                                                                                                                                                          <  1:370276/251‑1 (MQ=255)                                                          tCGGTGTGTTGTTCATTATTTGCCTCACTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGat                                                                                                                                                                                                >  1:505650/1‑242 (MQ=255)                                                                tgttggttattattTGCCTCCCTATACACCGGATTTACCCCAATCTTTGTTGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAAc                                                                                                                                                                                  <  1:179102/242‑1 (MQ=255)                                                                                ccTCACTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATAcc                                                                                                                                                                 <  1:841897/251‑1 (MQ=255)                                                                                ccTCACTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATAc                                                                                                                                                                  >  1:399636/1‑250 (MQ=255)                                                                                ccTCACTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATAc                                                                                                                                                                  <  1:763491/250‑1 (MQ=255)                                                                                  tCACTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGc                                                                                                                                                                                                                       <  1:127815/195‑1 (MQ=255)                                                                                  tCACTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCg                                                                                                                                                               >  1:146754/1‑251 (MQ=255)                                                                                  tCACTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCg                                                                                                                                                               >  1:167180/1‑251 (MQ=255)                                                                                   cACTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCgg                                                                                                                                                              >  1:372881/1‑251 (MQ=255)                                                                                    aCTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAg                                                                                                                                                                                                                              >  1:896938/1‑186 (MQ=255)                                                                                    aCTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGt                                                                                                                                                             >  1:827560/1‑251 (MQ=255)                                                                                    aCTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGt                                                                                                                                                             >  1:250014/1‑251 (MQ=255)                                                                                    aCTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGt                                                                                                                                                             >  1:209441/1‑251 (MQ=255)                                                                                    aCTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGt                                                                                                                                                             >  1:342768/1‑251 (MQ=255)                                                                                     cTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAAc                                                                                                                                                                                  <  1:91745/229‑1 (MQ=255)                                                                                     cTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATAc                                                                                                                                                                  <  1:511286/245‑1 (MQ=255)                                                                                         acacAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTc                                                                                                                                                                                                                                              >  1:97551/1‑165 (MQ=255)                                                                                         acacAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAAt                                                                                                                                                                                                          >  1:590952/1‑201 (MQ=255)                                                                                         acacAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATc                                                                                                                                                                                                         >  1:327441/1‑202 (MQ=255)                                                                                         acacAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGa                                                                                                                                                        >  1:556217/1‑251 (MQ=255)                                                                                              ggATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAAc                                                                                                                                                                                                                                >  1:158120/1‑174 (MQ=255)                                                                                              ggATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAgg                                                                                                                                                    >  1:683718/1‑250 (MQ=255)                                                                                               gATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCGGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATa                                                                                                                                                      <  1:214764/247‑1 (MQ=255)                                                                                                    tCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATAc                                                                                                                                                                  >  1:859188/1‑230 (MQ=255)                                                                                                    tCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAAt                                                                                                                                             <  1:325240/251‑1 (MQ=255)                                                                                                          aTCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGttaaatt                                                                                                                                                                        <  1:683047/218‑1 (MQ=255)                                                                                                           tCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACaa                                                                                                                                      <  1:838346/251‑1 (MQ=255)                                                                                                            cTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAAt                                                                                                                                     <  1:382302/251‑1 (MQ=255)                                                                                                                      aaCGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCAc                                                                                                                           <  1:374386/251‑1 (MQ=255)                                                                                                                      aaCGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCAc                                                                                                                           <  1:359033/251‑1 (MQ=255)                                                                                                                           ggAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCAc                                                                                                                                                                                          <  1:316704/183‑1 (MQ=255)                                                                                                                              gagTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACCGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGAc                                                                                                                                        >  1:547807/1‑230 (MQ=255)                                                                                                                              gagTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGAt                                                                                                                                                       >  1:261464/1‑215 (MQ=255)                                                                                                                               agtagtTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTgg                                                                                                                    <  1:306689/249‑1 (MQ=255)                                                                                                                                gtagtTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGgatg                                                                                                                 >  1:281121/1‑251 (MQ=255)                                                                                                                                gtagtTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGCGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCAc                                                                                                                           >  1:670804/1‑241 (MQ=255)                                                                                                                                 tagtTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGCTATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGgatga                                                                                                                >  1:427357/1‑251 (MQ=255)                                                                                                                                 tagtTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGgatg                                                                                                                 <  1:180803/250‑1 (MQ=255)                                                                                                                                         ccGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTc                                                                                                         <  1:71726/250‑1 (MQ=255)                                                                                                                                                      tgCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAGATTGAGcc                                                                                            <  1:188868/250‑1 (MQ=255)                                                                                                                                                                        ccAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCct                                                                         <  1:318263/251‑1 (MQ=255)                                                                                                                                                                         cAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGTCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCctc                                                                        <  1:395432/251‑1 (MQ=255)                                                                                                                                                                            aaaGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGc                                                                     <  1:373250/251‑1 (MQ=255)                                                                                                                                                                                         gaacAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAATGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCctc                                                                        <  1:422925/232‑1 (MQ=255)                                                                                                                                                                                         gATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGa                                                                   >  1:275327/1‑240 (MQ=255)                                                                                                                                                                                             aGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATGa                                                      >  1:275810/1‑249 (MQ=255)                                                                                                                                                                                              gCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTc                                                                                                                                                                                                                  <  1:384207/92‑1 (MQ=255)                                                                                                                                                                                                  tttCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTcc                                                                                                                              <  1:707515/172‑1 (MQ=255)                                                                                                                                                                                                  tttCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATg                                                       >  1:388928/1‑243 (MQ=255)                                                                                                                                                                                                  tttCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATg                                                       <  1:77177/243‑1 (MQ=255)                                                                                                                                                                                                  tttCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATGACTGGa                                                 >  1:397104/1‑249 (MQ=255)                                                                                                                                                                                                  tttCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATGACTGGa                                                 >  1:473021/1‑249 (MQ=255)                                                                                                                                                                                                       atatTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATGACTGGACTCTTgg                                          >  1:491496/1‑251 (MQ=255)                                                                                                                                                                                                         atTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATGACTGGACTCTTGGAc                                        <  1:748955/251‑1 (MQ=255)                                                                                                                                                                                                          ttGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATGACTGGACTCTTGGAcc                                       <  1:743921/251‑1 (MQ=255)                                                                                                                                                                                                           tGAGCCGAGCGGGGCAGTTTTGCGTATAAAAAGCCCGGATAGCAAAAAGTCCCGAAAAATTTTAAAGAAAGTGCAAATCGCTTAAAACCCCCCAGATATGCACTTTTCAAATGGTTAAATTAAAAACCCCGTGTGGGTAGGGGACAAAACGACAAAGTTTTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATGACTGGACTCTTGGACCt                                      <  1:755154/251‑1 (MQ=255)                                                                                                                                                                                                            gAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATGACTGGACTCTTGGAc                                        <  1:529684/248‑1 (MQ=255)                                                                                                                                                                                                            gAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATGACTGGACTCTTGGACCTa                                     >  1:284210/1‑251 (MQ=255)                                                                                                                                                                                                                    cGGGCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATGACTGGACTCTTGGACCTATCTCaaat                             <  1:340733/251‑1 (MQ=255)                                                                                                                                                                                                                      ggCCAGTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATg                                                       >  1:585446/1‑223 (MQ=255)                                                                                                                                                                                                                           gTTTTGCGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATGACTGGACTCTTGGAc                                        >  1:64383/1‑233 (MQ=255)                                                                                                                                                                                                                                 cGTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATGACTGGACTCTTGGACCTATCTCAAATAAATACGCATAAt                <  1:394476/251‑1 (MQ=255)                                                                                                                                                                                                                                  gTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATGACTGGACTCTTGGACCTATCTCaaataaa                          <  1:531098/240‑1 (MQ=255)                                                                                                                                                                                                                                  gTATCAAATGCCGGGATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATGACTGGACTCTTGGACCTATCTCAAATAAATACGCATAAtt               >  1:757318/1‑251 (MQ=255)                                                                                                                                                                                                                                                gATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCGCCCGAGATATGCACTTTCCAACTGGTTAAATTAAATTCCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATGACTGGACTCTTGGACCTATCTCAAATAAATACGCATAATTCATCAGATTAGGc  <  1:268081/250‑1 (MQ=255)                                                                                                                                                                                                                                                gATAGCAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATGACTGGACTCTTGGACCTATCTCAAATAAATACGCATAATTCATCAGATTAGGc  <  1:866152/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     GTGTGATAATAAAGCCGAGTATAAATTTTAAACGACGACGTGAAGGCTTCGTGTTATTCGGTGTGTTGTTCATTATTTGCCTCACTATACACAGGATTATCCCCAATCTTTGTGGATAACGGGGAGAGTAGTTGTGCCGGGAGTTGTTGTGCCAAAAATTGAATCAACCAAAAAGCATCAACGTGATCAGCAATTTCAATATTGAGCCGAGCGGGCCAGTTTTGCGTATCAAATGCCGGGATAGAAAAACGTCCGGAAACATTTAACAGAAAGTGCAATTCGCTTAAATCACCCGAGATATGCACTTTCCAACTGGTTAAATTAAATACCCGGTGCGGATAGGCGACAATACGACAATGTTGTCCCACTTTGTGGATGATTTTGTCTGCAAATTGAGCCAGGTTTTTTTGAATATCCTCTGCGACAAATTTTAATGACTGGACTCTTGGACCTATCTCAAATAAATACGCATAATTCATCAGATTAGGC  >  1/1694177‑1694665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑