Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,692,930	A→C	P326P (CCT→CCG)	unknown ←	putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,692,930	0	A	C	100.0%	185.2 / NA	54	P326P (CCT→CCG)	unknown	putative membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (26/28);  total (26/28)

GGCTAATTCCCACCAAAATGATGTTGTCACAATGGCAAAGTGCACAAATGTGATCGTAATCACGGTTTTCAACGAATAAGCACTCAAGATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTAGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCATATCGGCCAATAAACGTGGTCTTAATCCGGCTTCAGCATTATTCCACCACTGATCACAAGTCGGATAACCGCCATTACCGTTGTTTGGTAACCC  >  1/1692684‑1693172                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   ggCTAATTCCCACCAAAATGATGTTGTCACAATGGCAAAGTGCACAAATGTGATCGTAATCACGGTTTTCAACGAATAAGCACTCAAGATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGAt                                                                                                                                                                                                                                                >  1:5288/1‑251 (MQ=255)               aaaaTGATGTTGTCACAATGGCAAAGTGCACAAATGTGATCGTAATCACGGTTTTCAACGAATAAGCACTCAAGATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGt                                                                                                                                                                                                                                          >  1:467995/1‑243 (MQ=255)                             acaATGGCAAAGTGCACAAATGTGATCGTAATCACGGTTTTCAACGAATAAGCACTCAAGATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAAc                                                                                                                                                                                                                    <  1:319150/251‑1 (MQ=255)                                  ggCAAAGTGCACAAATGTGATCGTAATCACGGTTTTCAACGAATAAGCACTCAAGATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCTAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACtttt                                                                                                                                                                                                                >  1:282871/1‑250 (MQ=255)                                        gTGCACAAATGTGATCGTAATCACGGTTTTCAACGAATAAGCACTCAAGATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTgg                                                                                                                                                                                                         >  1:178320/1‑251 (MQ=255)                                          gCACAAATGTGATCGTAATCACGGTTTTCAACGAATAAGCACTCAAGATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGAt                                                                                                                                                                                                       >  1:817558/1‑251 (MQ=255)                                           cacaAATGTGATCGTAATCACGGTTTTCAACGAATAAGCACTCAAGATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGAt                                                                                                                                                                                                       <  1:507605/250‑1 (MQ=255)                                                     aTCGTAATCACGGTTTTCAACGAATAAGCACTCAAGATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATAtt                                                                                                                                                                                            >  1:710544/1‑251 (MQ=255)                                                                           aaTAAGCACTCAAGATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGAt                                                                                                                                                                                                       <  1:369462/218‑1 (MQ=255)                                                                           aaTAAGCACTCAAGATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTtct                                                                                                                                                                       >  1:167240/1‑250 (MQ=255)                                                                              aaGCACTCAAGATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGt                                                                                                                                                                   <  1:724835/251‑1 (MQ=255)                                                                              aaGCACTCAAGATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGt                                                                                                                                                                   <  1:632600/251‑1 (MQ=255)                                                                              aaGCACTCAAGATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGt                                                                                                                                                                   <  1:57972/251‑1 (MQ=255)                                                                                       aGATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGt                                                                                                                                                                                                                                          <  1:819723/171‑1 (MQ=255)                                                                                       aGATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGatat                                                                                                                                                                                             <  1:59263/216‑1 (MQ=255)                                                                                       aGATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGatat                                                                                                                                                                                             <  1:39542/216‑1 (MQ=255)                                                                                        gATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCAt                                                                                                                                                         >  1:169591/1‑251 (MQ=255)                                                                                                               tGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTaaaa                                                                                                                                  >  1:133616/1‑251 (MQ=255)                                                                                                                gATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACTAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTtctc                                                                                                                                                                      >  1:591408/1‑214 (MQ=255)                                                                                                                gATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCa                                                                                                                                                                     >  1:689313/1‑215 (MQ=255)                                                                                                                gATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTaaaaa                                                                                                                                 >  1:489794/1‑251 (MQ=255)                                                                                                                        ccaTAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTtc                                                                                                                                                                        <  1:470359/204‑1 (MQ=255)                                                                                                                          aTAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGAc                                                                                                                       <  1:398880/251‑1 (MQ=255)                                                                                                                           tAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACt                                                                                                                      <  1:348328/251‑1 (MQ=255)                                                                                                                                  aaaTGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCgg                                                                                                               <  1:366339/251‑1 (MQ=255)                                                                                                                                     tGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGaaa                                                                                                            >  1:473174/1‑251 (MQ=255)                                                                                                                                          gTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACt                                                                                                                      <  1:121865/236‑1 (MQ=255)                                                                                                                                              catcGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGt                                                                                                    >  1:372456/1‑250 (MQ=255)                                                                                                                                               atcGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTTTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCg                                                                                                  <  1:424215/251‑1 (MQ=255)                                                                                                                                                 cGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCa                                                                                                >  1:192611/1‑251 (MQ=255)                                                                                                                                                  ggTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGa                                                                                                                                                                                                                                                 >  1:619693/1‑105 (MQ=255)                                                                                                                                                   gTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACt                                                                                                                      <  1:294319/227‑1 (MQ=255)                                                                                                                                                      aTGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCATATCg                                                                                           >  1:671005/1‑251 (MQ=255)                                                                                                                                                        gCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCATATCgg                                                                                          >  1:496226/1‑250 (MQ=255)                                                                                                                                                                   tGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCATATCGGCCAATAAACg                                                                                <  1:504994/249‑1 (MQ=255)                                                                                                                                                                                gggAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCatat                                                                                             <  1:563085/223‑1 (MQ=255)                                                                                                                                                                                   aaaaTGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCATATCGGCCAATAAACGTGGTCTTAATCCGGCtt                                                               >  1:556017/1‑250 (MQ=255)                                                                                                                                                                                       tGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAGCTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAg                                                                                                                               <  1:344948/182‑1 (MQ=255)                                                                                                                                                                                       tGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCa                                                                                                                                                                     >  1:901122/1‑144 (MQ=255)                                                                                                                                                                                       tGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAg                                                                                                                                                                 >  1:27632/1‑148 (MQ=255)                                                                                                                                                                                              aCTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCATATCGGCCAATAAACGTGGTCTTAATCCGGCTTCAGCattat                                                      <  1:655578/248‑1 (MQ=255)                                                                                                                                                                                                 gCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGACAGGTTTTGTCGCatat                                                                                             >  1:761101/1‑206 (MQ=255)                                                                                                                                                                                                 gCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCATATCGGCCAATAAACGTGGTCTTAATCCGGCTTCAGCATTATTccac                                                 >  1:321676/1‑250 (MQ=255)                                                                                                                                                                                                 gCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCATATCGGCCAATAAACGTGGTCTTAATCCGGCTTCAGCATTATTccac                                                 >  1:322314/1‑250 (MQ=255)                                                                                                                                                                                                 gCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCATATCGGCCAATAAACGTGGTCTTAATCCGGCTTCAGCATTATTccac                                                 <  1:790723/250‑1 (MQ=255)                                                                                                                                                                                                   ccACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTTTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCATATCGGCCAATAAACGTGGTCTTAATCCGGCTTCAGCATTATTCcaccac                                              <  1:611299/251‑1 (MQ=255)                                                                                                                                                                                                   ccACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCATATCGGCCAATAAACGTGGTCTTAATCCGGCTTCAGCATTATTCcaccac                                              <  1:853589/251‑1 (MQ=255)                                                                                                                                                                                                     acaAGGGAACCCGCGCCGGCAGCGGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGAGCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGAATATCGGCCAATAAACGTGGTCTTAATCCGGCTTCAGCATTATTCCACCACTg                                            <  1:578778/251‑1 (MQ=255)                                                                                                                                                                                                      caAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCATATCGGCCAATAAACGTGGTCTTAATCCGGCTTCAGCATTATTCcaccac                                              <  1:250696/248‑1 (MQ=255)                                                                                                                                                                                                            aaCCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCATATCGGCCAATAAACGTGGTCTTAATCCGGCTTCAGCATTATTCcaccac                                              <  1:41584/242‑1 (MQ=255)                                                                                                                                                                                                                     cGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCATATCGGCCAATAAACGTGGTCTTAATCCGGCTTCAGCATTATTCCACCACTGATCACAAGTCGGATaa                            >  1:228641/1‑251 (MQ=255)                                                                                                                                                                                                                           cagTTCTGGCTGCAGCATTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCATATCGGCCAATAAACGTGGTCTTAATCCGGCTTCAGCATTATTCCACCACTGATCACAAGTCGGATAACCg                         <  1:222425/248‑1 (MQ=255)                                                                                                                                                                                                                                           aTTTGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCATATCGGCCAATAAACGTGGTCTTAATc                                                                     <  1:76829/188‑1 (MQ=255)                                                                                                                                                                                                                                              tGCCATGGTCGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCATATCGGCCAATAAACGTGGTCTTAATCCGGCTTCAGCATTATTCCACCACTGATCACAAGTc                                  >  1:707980/1‑220 (MQ=255)                                                                                                                                                                                                                                                ccgtggtggGATCAGGTTTGCCCCCGTAACTGGGATAATTTTTTCTTTTGTATACATGGTTTTTTGCGGGGGGGAGTAGTTTTCTAGGTGGGGTTTCATTTTTATTACCGGCCGATTAAAAAAAGCCACTGCTTTGGGCGAAAAGTTTTTGTCGCATATCGGCCAAAAAACGTGGTCTTAACCCGGCTTCAGCATTATCCCCCCACTGACCACAGGGAGAATAACCGCCATTACCGTTGTTTGGTAAccc  <  1:239495/247‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   GGCTAATTCCCACCAAAATGATGTTGTCACAATGGCAAAGTGCACAAATGTGATCGTAATCACGGTTTTCAACGAATAAGCACTCAAGATAATGATTGCCGGTGTCAGAATGATAACCACCATAATCAAAAATGCCTGTATCATCGGTAATGCTTGGCGTAGTGAATCAAGTCCGGGGAAAATGGCCAGACTGCCCACAAGGGAACCCGCGCCGGCAGCAGTTCTGGCTGCAGCATTTGCCATGGTAGGATCAAGGTTGCCACCGTAACTCGGATAAACTTTTCCTTTGGATACATCGATATTTTCCGGGCGGAGTAGTGTTCTCAGTAGGGCTTCATCTTGATTATCGACCGAGTTAAAAAAGTCACTGACTTGTGCGGAAAGGTTTTGTCGCATATCGGCCAATAAACGTGGTCTTAATCCGGCTTCAGCATTATTCCACCACTGATCACAAGTCGGATAACCGCCATTACCGTTGTTTGGTAACCC  >  1/1692684‑1693172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑