Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	333,842	T→C	I164I (ATT→ATC)	unknown →	Manganese ABC transporter, periplasmic‑binding protein SitA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	333,842	0	T	C	100.0%	281.3 / NA	81	I164I (ATT→ATC)	unknown	Manganese ABC transporter, periplasmic‑binding protein SitA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/42);  total (39/42)

CCTTGAGCGTTGGTTTGAAAAATTCTTCCAAAACGTCAAAGATAAACCGGCGGTCGTTGTAACCGAAGGCATTGAGCCGATGTCAATTCATGAAGGTCCGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATTAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTGAAACCGTCAAAGCCAACAATATTCCGGTCGTATTCAGTGAAAGCACCATCTCCCCGAAAC  >  1/333596‑334083                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  ccTTGAGCGTTGGTTTGAAAAATTCTTCCAAAACGTCAAAGATAAACCGGCGGTCGTTGTAACCGAAGGCATTGAGCCGATGTCAATTCATGAAGGTCCGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAg                                                                                                                                                                                                                                               <  1:108270/251‑1 (MQ=255)              tttGAAAAATTCTTCCAAAACGTCAAAGATAAACCGGCGGTCGTTGTAACCGAAGGCATTGAGCCGATGTCAATTCATGAAGGTCCGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAAcc                                                                                                                                                                                                                                  <  1:525906/251‑1 (MQ=255)              tttGAAAAATTCTTCCAAAACGTCAAAGATAAACCGGCGGTCGTTGTAACCGAAGGCATTGAGCCGATGTCAATTCATGAAGGTCCGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAAc                                                                                                                                                                                                                                   <  1:352336/250‑1 (MQ=255)                tGAAAAATTCTTCCAAAACGTCAAAGATAAACCGGCGGTCGTTGTAACCGAAGGCATTGAGCCGATGTCAATTCATGAAGGTCCGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGt                                                                                                                                                                                                                                <  1:565773/251‑1 (MQ=255)                                   gTCAAAGATAAACCGGCGGTCGTTGTAACCGAAGGCATTGAGCCGATGTCAATTCATGAAGGTCCGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGcaca                                                                                                                                                                                                             >  1:514383/1‑251 (MQ=255)                                            aaaCCGGCGGTCGTTGTAACCGAAGGCATTGAGCCGATGTCAATTCATGAAGGTCCGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGa                                                                                                                                                                                                    <  1:615666/251‑1 (MQ=255)                                                         ttGTAACCGAAGGCATTGAGCCGATGTCAATTCATGAAGGTCCGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCgttgg                                                                                                                                                                                       >  1:623296/1‑251 (MQ=255)                                                            tAACCGAAGGCATTGAGCCGATGTCAATTCATGAAGGTCCGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCgttggtt                                                                                                                                                                                     <  1:515200/250‑1 (MQ=255)                                                            tAACCGAAGGCATTGAGCCGATGTCAATTCATGAAGGTCCGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCgttggtt                                                                                                                                                                                     <  1:668043/250‑1 (MQ=255)                                                                     gCATTGAGCCGATGTCAATTCATGAAGGTCCGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGcac                                                                                                                                                                                                              >  1:323350/1‑216 (MQ=255)                                                                     gCATTGAGCCGATGTCAATTCATGAAGGTCCGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCgt                                                                                                                                                                                          >  1:31272/1‑236 (MQ=255)                                                                     gCATTGAGCCGATGTCAATTCATGAAGGTCCGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAg                                                                                                                                                                            >  1:96428/1‑250 (MQ=255)                                                                          gAGCCGATGTCAATTCATGAAGGTCCGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGt                                                                                                                                                                                                                                >  1:901828/1‑193 (MQ=255)                                                                                         cATGAAGGTCCGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTAttt                                                                                                                                                       <  1:533656/251‑1 (MQ=255)                                                                                         cATGAAGGTCCGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTAttt                                                                                                                                                       <  1:160301/251‑1 (MQ=255)                                                                                               ggTCCGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACg                                                                                                                                                 >  1:335477/1‑251 (MQ=255)                                                                                                   cGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGAt                                                                                                                                             <  1:254262/251‑1 (MQ=255)                                                                                                     tACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGa                                                                                                                                                                                                    <  1:820445/194‑1 (MQ=255)                                                                                                        acCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACgg                                                                                                                                        <  1:24386/251‑1 (MQ=255)                                                                                                        acCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGAGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACgg                                                                                                                                        <  1:485879/251‑1 (MQ=255)                                                                                                         ccGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGt                                                                                                                                       >  1:357602/1‑251 (MQ=255)                                                                                                         ccGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGt                                                                                                                                       <  1:786036/251‑1 (MQ=255)                                                                                                                       ccgCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACgg                                                                                                                                        <  1:542544/236‑1 (MQ=255)                                                                                                                            tGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTtgt                                                                                                                    >  1:833014/1‑251 (MQ=255)                                                                                                                            tGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTtgt                                                                                                                    >  1:113499/1‑251 (MQ=255)                                                                                                                             gCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTAtt                                                                                                                       >  1:332579/1‑247 (MQ=255)                                                                                                                               cTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGc                                                                                                                 <  1:876763/251‑1 (MQ=255)                                                                                                                                   aTGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAg                                                                                                                                                     <  1:493033/211‑1 (MQ=255)                                                                                                                                         ccTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAag                                                                                                                                >  1:582904/1‑226 (MQ=255)                                                                                                                                         ccTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTtg                                                                                                                     <  1:675265/237‑1 (MQ=255)                                                                                                                                         ccTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGc                                                                                                       <  1:168243/251‑1 (MQ=255)                                                                                                                                           ttCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACg                                                                                                        >  1:428549/1‑248 (MQ=255)                                                                                                                                             ccAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAg                                                                                                   >  1:133717/1‑251 (MQ=255)                                                                                                                                                  gCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGAt                                                                                                                                                                                                                                       <  1:423285/114‑1 (MQ=255)                                                                                                                                                  gCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAg                                                                                                                                                                                                  >  1:504200/1‑151 (MQ=255)                                                                                                                                                  gCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTAttt                                                                                                                      <  1:897465/227‑1 (MQ=255)                                                                                                                                                  gCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGcaaca                                                                                              >  1:736969/1‑251 (MQ=255)                                                                                                                                                  gCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGcaaca                                                                                              >  1:170254/1‑251 (MQ=255)                                                                                                                                                    gTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAg                                                                                            >  1:722583/1‑251 (MQ=255)                                                                                                                                                     ttAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAgg                                                                                           <  1:562069/251‑1 (MQ=255)                                                                                                                                                      tAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATTTCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGc                                                                                          <  1:688238/251‑1 (MQ=255)                                                                                                                                                      tAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGc                                                                                          <  1:261980/251‑1 (MQ=255)                                                                                                                                                            aTGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAg                                                                                                                                                     >  1:819109/1‑186 (MQ=255)                                                                                                                                                                     atatTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTg                                                                           >  1:237039/1‑251 (MQ=255)                                                                                                                                                                      tatTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTaa                                                                                                          <  1:107580/219‑1 (MQ=255)                                                                                                                                                                      tatTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGc                                                                                                       >  1:525584/1‑222 (MQ=255)                                                                                                                                                                            aaaTGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAg                                                                    <  1:749569/251‑1 (MQ=255)                                                                                                                                                                                     gATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTg                                                                                                                                                                                                                              >  1:165758/1‑88 (MQ=255)                                                                                                                                                                                     gATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGcac                                                                                                                                                                                                              >  1:587952/1‑104 (MQ=255)                                                                                                                                                                                     gATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGgcg                                                                                                                                                                    >  1:414349/1‑146 (MQ=255)                                                                                                                                                                                     gATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGgcg                                                                                                                                                                    >  1:340914/1‑146 (MQ=255)                                                                                                                                                                                     gATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAg                                                                                                                                                     >  1:134557/1‑161 (MQ=255)                                                                                                                                                                                     gATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGcaacaa                                                                                             <  1:357936/217‑1 (MQ=255)                                                                                                                                                                                     gATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTGaaa                                                           >  1:15017/1‑251 (MQ=255)                                                                                                                                                                                     gATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTGaaa                                                           >  1:400792/1‑251 (MQ=255)                                                                                                                                                                                     gATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTGaaa                                                           >  1:663173/1‑251 (MQ=255)                                                                                                                                                                                     gATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATACAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTGaa                                                            >  1:802485/1‑250 (MQ=255)                                                                                                                                                                                     gATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTCTATATCCAAAAAATCAAAGAATTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGt                                                                   >  1:162310/1‑243 (MQ=255)                                                                                                                                                                                      aTTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAag                                                                                                                                <  1:30194/181‑1 (MQ=255)                                                                                                                                                                                                  ccGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTGAAACCGTCAAAGCcaa                                              >  1:278414/1‑251 (MQ=255)                                                                                                                                                                                                       aaaTAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAg                                                                             >  1:765279/1‑215 (MQ=255)                                                                                                                                                                                                         aTAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGAcc                                                                                                                                                                              <  1:545689/116‑1 (MQ=255)                                                                                                                                                                                                          tAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAag                                                                                                                                >  1:306781/1‑161 (MQ=255)                                                                                                                                                                                                          tAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTGAAACCGTCAAAGCCAACAATATTc                                      <  1:743200/251‑1 (MQ=255)                                                                                                                                                                                                          tAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTGAAACCGTCAAAGCCAACAATATTc                                      <  1:211807/251‑1 (MQ=255)                                                                                                                                                                                                              gAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTGAAACCGTCAAAGCCAACAATATTc                                      >  1:137055/1‑247 (MQ=255)                                                                                                                                                                                                               aaaCATACGAAAAAAATACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTGAAACCGTCAAAGCCAACAATATTCCgg                                   <  1:320564/249‑1 (MQ=255)                                                                                                                                                                                                                         aaaaaaTACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTGAAACCGTCAAAGCCAACAATATTCCGGTCGTATTCAGTg                       >  1:76389/1‑251 (MQ=255)                                                                                                                                                                                                                         aaaaaaTACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGCAATTGAAACCGTCAAAGCCAACAATATTCCGGTCGTATTCAGTg                       >  1:652531/1‑251 (MQ=255)                                                                                                                                                                                                                              aTACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGc                                                                                                       <  1:865739/166‑1 (MQ=255)                                                                                                                                                                                                                              aTACCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTg                                                              <  1:20534/207‑1 (MQ=255)                                                                                                                                                                                                                                aCCGCACTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTGAAACCGTCAAAGCCAACAATATTCCGGTCGTATTCAGTGAAAGCAc                <  1:664785/251‑1 (MQ=255)                                                                                                                                                                                                                                      cTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAg                                                                                                                                                                                                  <  1:574294/67‑1 (MQ=255)                                                                                                                                                                                                                                      cTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGAt                                                                                                                                             <  1:856546/120‑1 (MQ=255)                                                                                                                                                                                                                                      cTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGcaac                                                                                <  1:778230/181‑1 (MQ=255)                                                                                                                                                                                                                                      cTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTGAAACCGTCAAAGCCAACAATATTCCGGTCGTATTCAGTGAAAGCACCAtct           >  1:351060/1‑250 (MQ=255)                                                                                                                                                                                                                                      cTTTATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTGAAACCGTCAAAGCCAACAATATTCCGGTCGTATTCAGTGAAAGCACCAtct           >  1:331518/1‑250 (MQ=255)                                                                                                                                                                                                                                        ttATATCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTGAAAc                                                          <  1:523051/201‑1 (MQ=255)                                                                                                                                                                                                                                            atCCAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTGAAACCGTCAAAGCCAACAATATTCCGGTCGTATTCAGTGAAAGCACCATCTCCCCGaa    <  1:803306/251‑1 (MQ=255)                                                                                                                                                                                                                                              ccAAAAAATCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTTTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTGAAACCGTCAAAGCCAACAATATTCCGGTCGTATTCAGTGAAAGCACCATCTCCCCGAAAc  <  1:820792/251‑1 (MQ=255)                                                                                                                                                                                                                                                   aaaTCAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCgttggtt                                                                                                                                                                                     <  1:373500/67‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  CCTTGAGCGTTGGTTTGAAAAATTCTTCCAAAACGTCAAAGATAAACCGGCGGTCGTTGTAACCGAAGGCATTGAGCCGATGTCAATTCATGAAGGTCCGTACACCGGCAACCCGAACCCGCATGCCTGGATGTCACCTTCCAATGCGTTAATCTATGTTGAAAATATTAAAAATGCGTTGATTAAATACGATCCGCAAAATAAAGAAACATACGAAAAAAATACCGCACTTTATATCCAAAAAATTAAAGAACTTGATCAACCGTTGCGTGAAAAATTAGCACAAGTGCCGGAAGCACAGCGTTGGTTGGTGACCAGCGAAGGCGCATTCAGTTATTTAGCACGTGATTACGGTTTTAAAGAGGCTTATTTGTGGCCGATTAACGCTGAGCAACAAGGCACGCCGCAACAAGTGCGTAAAGTAATTGAAACCGTCAAAGCCAACAATATTCCGGTCGTATTCAGTGAAAGCACCATCTCCCCGAAAC  >  1/333596‑334083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑