Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,651,779	C→T	T27T (ACG→ACA)	unknown ←	tRNA (cytidine(32)/uridine(32)‑2'‑O)‑methyltransferase (EC 2.1.1.200)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,651,779	0	C	T	100.0%	170.8 / NA	51	T27T (ACG→ACA)	unknown	tRNA (cytidine(32)/uridine(32)‑2'‑O)‑methyltransferase (EC 2.1.1.200)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/33);  total (18/33)

CTTTCCCGTTTGTTGCGTAAGCAACGGCTTTTTTGCCGCACTCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATCGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGATTTAATATATTAGCTATTCTAACATAATACCCTTTATTTTTAGTCAACAATTCTAGAGATTTTTCTGATAGCTATTTCCTTATAAACTGCTTATAATTTGAGATCCGATTGTTCTTTAAAATCTGGTGGAATTTATGAATCCAATGT  >  1/1651535‑1652022                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    cTTTCCCGTTTGTTGCGTAAGCAACGGCTTTTTTGCCGCACTCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCtt                                                                                                                                                                                                                                                >  1:518248/1‑250 (MQ=255)   ttCCCGTTTGTTGCGTAAGCAACGGCTTTTTTGCCGCACTCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGCAAATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCa                                                                                                                                                                                                                                              >  1:636777/1‑251 (MQ=255)    tCCCGTTTGTTGCGTAAGCAACGGCTTTTTTGCCGCACTCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCAtt                                                                                                                                                                                                                                            <  1:836661/251‑1 (MQ=255)     cccGTTTGTTGCGTAAGCAACGGCTTTTTTGCCGCACTCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTg                                                                                                                                                                                                                                           >  1:852981/1‑251 (MQ=255)         tttGTTGCGTAAGCAACGGCTTTTTTGCCGCACTCACGCGGTTCAATAAGCGAGTTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCg                                                                                                                                                                                                                                       >  1:79606/1‑251 (MQ=255)             ttgCGTAAGCAACGGCTTTTTTGCCGCACTCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTgc                                                                                                                                                                                                                                    <  1:103044/250‑1 (MQ=255)               gCGTAAGCAACGGCTTTTTTGCCGCACTCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTgcag                                                                                                                                                                                                                                  >  1:626243/1‑250 (MQ=255)                  tAAGCAACGGCTTTTTTGCCGCACTCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGcagcag                                                                                                                                                                                                                               <  1:194653/250‑1 (MQ=255)                      cAACGGCTTTTTTGCCGCACTCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAAc                                                                                                                                                                                                                            <  1:85661/249‑1 (MQ=255)                      cAACGGCTTTTTTGCCGCACTCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCg                                                                                                                                                                                                                          <  1:32039/251‑1 (MQ=255)                         cGGCTTTTTTGCCGCACTCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCATCACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATg                                                                                                                                                                                                                       <  1:598992/251‑1 (MQ=255)                            cTTTTTTGCCGCACTCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCg                                                                                                                                                                                                                          <  1:516725/245‑1 (MQ=255)                                    ccGCACTCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCtgtg                                                                                                                                                                                                            >  1:385508/1‑251 (MQ=255)                                         cTCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAgg                                                                                                                                                                                                       <  1:806367/251‑1 (MQ=255)                                          tCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGGTCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGt                                                                                                                                                                                                      <  1:343378/251‑1 (MQ=255)                                          tCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGt                                                                                                                                                                                                      <  1:421066/251‑1 (MQ=255)                                          tCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGt                                                                                                                                                                                                      <  1:732731/251‑1 (MQ=255)                                          tCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGt                                                                                                                                                                                                      <  1:9929/251‑1 (MQ=255)                                               cgGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGt                                                                                                                                                                                                      <  1:209598/246‑1 (MQ=255)                                                      aTAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAAc                                                                                                                                                                                          <  1:366768/251‑1 (MQ=255)                                                      aTAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTCCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAACAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAAc                                                                                                                                                                                          <  1:425551/251‑1 (MQ=255)                                                                 cTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAAtt                                                                                                                                                                                               >  1:666034/1‑235 (MQ=255)                                                                         ggCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGcc                                                                                                                                                                                                                                         >  1:268252/1‑185 (MQ=255)                                                                               aTCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGt                                                                                                                                                                                                      <  1:649801/214‑1 (MQ=255)                                                                               aTCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACattatt                                                                                                                                                                 >  1:837475/1‑251 (MQ=255)                                                                                tCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTAttt                                                                                                                                                                <  1:292970/251‑1 (MQ=255)                                                                                     cgcTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGt                                                                                                                                                                                                      >  1:860463/1‑208 (MQ=255)                                                                                     cgcTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTtact                                                                                                                                                           <  1:733012/251‑1 (MQ=255)                                                                                          gTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGttt                                                                                                                                                                                                    >  1:284785/1‑205 (MQ=255)                                                                                            aCCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGAt                                                                                                                                                    <  1:221519/251‑1 (MQ=255)                                                                                                   aCTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGATTTAatat                                                                                                                                             <  1:479748/251‑1 (MQ=255)                                                                                                   aCTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGATTTAatat                                                                                                                                             <  1:382332/251‑1 (MQ=255)                                                                                                    cTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGt                                                                                                                                                                                                      <  1:250324/193‑1 (MQ=255)                                                                                                    cTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGATTTAatata                                                                                                                                            >  1:732580/1‑251 (MQ=255)                                                                                                                    cGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGATTTAATATATTAGCTATTCTAACa                                                                                                                             <  1:618730/251‑1 (MQ=255)                                                                                                                    cGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGATTTAATATATTAGCTATTCTAACa                                                                                                                             <  1:54422/251‑1 (MQ=255)                                                                                                                      aCCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGATTTAATATATTAGCTATTCTAACat                                                                                                                            <  1:740540/250‑1 (MQ=255)                                                                                                                             gtgAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAAc                                                                                                                                                                                          <  1:676141/181‑1 (MQ=255)                                                                                                                                                       cTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGATTTAATATATTAGCt                                                                                                                                      >  1:399802/1‑207 (MQ=255)                                                                                                                                                         tttCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGATTTAATATATTAGCTATTCTAACat                                                                                                                            >  1:383539/1‑215 (MQ=255)                                                                                                                                                            caccacATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGATTTAATATATTAGCTATTCTAACATAATACCCTTTA‑TTTTAGTCAACAATTCTAGAGATTTTTc                                                                                    <  1:76886/251‑1 (MQ=255)                                                                                                                                                              ccacATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGt                                                                                                                                                                                                      >  1:18184/1‑136 (MQ=255)                                                                                                                                                              ccacATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAAc                                                                                                                                                                       <  1:573586/167‑1 (MQ=255)                                                                                                                                                                                       ggCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGATTTAATATATTAGCTATTCTAACATAATACCCTTTATTTTTAGTCAACAATTCTAGAGATTTTTCTGATAGCTATTTCCTTATAAACTg                                                            >  1:670025/1‑249 (MQ=255)                                                                                                                                                                                                  gATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAAc                                                                                                                                                                                          <  1:16433/112‑1 (MQ=255)                                                                                                                                                                                                  gATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGATTTAATATATTAGCTATTCTAACat                                                                                                                            <  1:673093/174‑1 (MQ=255)                                                                                                                                                                                                  gATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGATTTAATATATTAGCTATTCTAACa                                                                                                                             <  1:549516/173‑1 (MQ=255)                                                                                                                                                                                                  gATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGATTTAATATATTAGCTATTCTAACATAATACCCTTTATTTTTAGTCAACAATTCTAGAGATTTTTCTGATAGCTATTTCCTTATAAACTGCTTATAATTTgag                                               >  1:782590/1‑251 (MQ=255)                                                                                                                                                                                                                           gACCAACACTAAATCCGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGATTTAATATATTAGCTATTCTAACATAATACCCTTTATTTTTAGTCAACAATTCTAGAGATTTTTCTGATAGCTATTTcc                                                                      <  1:816848/203‑1 (MQ=255)                                                                                                                                                                                                                                         ccGTTAATCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGATTTAATATATTAGCTATTCTAACATAATACCCTTTATTTTTAGTCAACAATTCTAGAGATTTTTCTGATAGCTATTTCCTTATAAAc                                                              <  1:542478/197‑1 (MQ=255)                                                                                                                                                                                                                                               aTCCCATTGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGGGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGATTTAATATATTAGCTATTCTAACATAATACCCTTTATTTTTAGTCAACAATTCTAGAGATTTTTCTGATAGCTATTTCCTTATAAACTGCTTATAATTTGAGATCCGATTGTTCTTTAAAATCTGGTGGAATTTATGAATCCAATGt  >  1:792647/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    CTTTCCCGTTTGTTGCGTAAGCAACGGCTTTTTTGCCGCACTCACGCGGTTCAATAAGCGAATTCTGCAAATGGCGCAATCTTGCGCTGGTACCGATGACTAAAGAGC‑AATCGGCGACCGCTTGTGAAAAGCTTTCCGCTGTGACCGCACTTTTCACCACATCTTCCGCACCGGCGGCAAGGGCGATAGCCTGATCATCCTCGCTTTGTTTCGGCGCGACCAACACTAAATCCGTTAATCCCATCGTCTTCATTGCCCGTGCAGCAGAACCGATGTTGCCGCTGTGTGAGGTTTCAATTAAAACAATCCGAATATTTTTTAACATTATTTTTACTACATGATTTAATATATTAGCTATTCTAACATAATACCCTTTATTTTTAGTCAACAATTCTAGAGATTTTTCTGATAGCTATTTCCTTATAAACTGCTTATAATTTGAGATCCGATTGTTCTTTAAAATCTGGTGGAATTTATGAATCCAATGT  >  1/1651535‑1652022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑