Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,613,290	G→A	S453F (TCT→TTT)	unknown ←	tRNA (5‑methylaminomethyl‑2‑thiouridylate)‑methyltransferase (EC 2.1.1.61) / FAD‑dependent cmnm(5)s(2)U34 oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,613,290	0	G	A	100.0%	256.5 / NA	76	S453F (TCT→TTT)	unknown	tRNA (5‑methylaminomethyl‑2‑thiouridylate)‑methyltransferase (EC 2.1.1.61) / FAD‑dependent cmnm(5)s(2)U34 oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/44);  total (32/44)

TCGCTAAAGCGACGATCGGCGCAATCGCGCACATGGCTGGCACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAGAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGATAAATCCGCCGCCACAAGGCAACGGCAAACCGACTTTTTCGCTTAATTCCGCCTGCGTTAAGGCTTGATAAAGATCGGTGGGCAAGTGAT  >  1/1613050‑1613536                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                        tCGCTAAAGCGACGATCGGCGCAATCGCGCACATGGCTGGCACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGcc                                                                                                                                                                                                                                               <  1:56627/251‑1 (MQ=255)  cGCTAAAGCGACGATCGGCGCAATCGCGCACATGGCTGGCACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGc                                                                                                                                                                                                                                                >  1:147662/1‑249 (MQ=255)            acgaTCGGCGCAATCGCGCACATGGCTGGCACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTgg                                                                                                                                                                                                                                    <  1:890507/251‑1 (MQ=255)            acgaTCGGCGCAATCGCGCACATGGCTGGCACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTgg                                                                                                                                                                                                                                    <  1:479762/251‑1 (MQ=255)            acgaTCGGCGCAATCGCGCACATGGCTGGCACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTgg                                                                                                                                                                                                                                    <  1:208826/251‑1 (MQ=255)            acgaTCGGCGCAATCGCGCACATGGCTGGCACCGATGCAATGGCTGGTTTGTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTgg                                                                                                                                                                                                                                    <  1:55401/251‑1 (MQ=255)              gaTCGGCGCAATCGCGCACATGGCTGGCACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTg                                                                                                                                                                                                                                  >  1:557675/1‑251 (MQ=255)                 gtgtttaccgctctttccctcattcACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAg                                                                                                                                                                                                                                >  1:394895/25‑250 (MQ=255)                 cGGCGCAATCGCGCACATGGCTGGCACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTTGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGGAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAg                                                                                                                                                                                                                                >  1:644653/1‑250 (MQ=255)                      cAATCGCGCACATGGCTGGCACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGcc                                                                                                                                                                                                                           <  1:533809/250‑1 (MQ=255)                        aTCGCGCACATGGCTGGCACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGt                                                                                                                                                                                                                        >  1:614621/1‑251 (MQ=255)                            cgcACATGGCTGGCACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGt                                                                                                                                                                                                                                   <  1:752427/236‑1 (MQ=255)                            cgcACATGGCTGGCACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGt                                                                                                                                                                                                                     <  1:160867/250‑1 (MQ=255)                            cgcACATGGCTGGCACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGt                                                                                                                                                                                                                     <  1:608011/250‑1 (MQ=255)                                caTGGCTGGCACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGcc                                                                                                                                                                                                                 >  1:634379/1‑250 (MQ=255)                                  tggctggcACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAg                                                                                                                                                                                                              >  1:228458/1‑251 (MQ=255)                                   ggctggcACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTc                                                                                                                                                                                                                                         >  1:181304/1‑223 (MQ=255)                                    gctggcACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGc                                                                                                                                                                                                             >  1:183843/1‑250 (MQ=255)                                         cACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑aa                                                                                                                                                                                                      <  1:543217/251‑1 (MQ=255)                                         cACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑aa                                                                                                                                                                                                      <  1:459731/251‑1 (MQ=255)                                           ccGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAg                                                                                                                                                                                                    <  1:839769/251‑1 (MQ=255)                                           ccGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAg                                                                                                                                                                                                    <  1:611660/251‑1 (MQ=255)                                           ccGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAg                                                                                                                                                                                                    <  1:868254/251‑1 (MQ=255)                                           ccGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAg                                                                                                                                                                                                    >  1:382871/1‑251 (MQ=255)                                                       cTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCAttt                                                                                                                                                                                         <  1:833771/250‑1 (MQ=255)                                                          gTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGTCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCAtttttt                                                                                                                                                                                      >  1:318083/1‑250 (MQ=255)                                                                     aTCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTa                                                                                                                                                                          <  1:457073/251‑1 (MQ=255)                                                                       ccGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAg                                                                                                                                                                        <  1:506719/251‑1 (MQ=255)                                                                            ggCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGCACTGAGGGAGCTGATGTCCGCTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCGGTGGTCGCACAGCCATGAAAAGCGCGGTCATTTTTTCCTGCGTTTTTAAGATCa                                                                                                                                                                    >  1:387803/1‑251 (MQ=255)                                                                            ggCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAg                                                                                                                                                                        >  1:517157/1‑246 (MQ=255)                                                                            ggCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCaa                                                                                                                                                                   >  1:164211/1‑251 (MQ=255)                                                                                  agaTAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGcc                                                                                                                                                                >  1:707599/1‑248 (MQ=255)                                                                                  agaTAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGcc                                                                                                                                                                >  1:260582/1‑248 (MQ=255)                                                                                          gtcgtAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTCAAGATCAAGCCCtgttgttg                                                                                                                                                       >  1:626390/1‑249 (MQ=255)                                                                                           tcgtAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATGGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTAATGTCCGTTCGCCAGCTCCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTa                                                                                                                                                     <  1:887702/250‑1 (MQ=255)                                                                                           tcgtAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGTGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGTGTTTTTAAGATCAAGCCCTGTTGTTGTa                                                                                                                                                     <  1:428615/250‑1 (MQ=255)                                                                                           tcgtAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑aa                                                                                                                                                                                                      >  1:595765/1‑201 (MQ=255)                                                                                           tcgtAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTa                                                                                                                                                     <  1:96262/250‑1 (MQ=255)                                                                                           tcgtAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTa                                                                                                                                                     <  1:272868/250‑1 (MQ=255)                                                                                           tcgtAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTa                                                                                                                                                     <  1:306946/250‑1 (MQ=255)                                                                                           tcgtAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTCGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTa                                                                                                                                                     >  1:658158/1‑250 (MQ=255)                                                                                              tAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTg                                                                                                                                                 >  1:529723/1‑251 (MQ=255)                                                                                                gCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTg                                                                                                                                                                                                  <  1:822433/200‑1 (MQ=255)                                                                                                          gATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTa                                                                                                                                                     <  1:470085/235‑1 (MQ=255)                                                                                                              tCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGc                                                                                                                                 <  1:12960/251‑1 (MQ=255)                                                                                                                               tCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGgcgc                                                                                                                <  1:133267/251‑1 (MQ=255)                                                                                                                                          aaTTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGccc                                                                                                     <  1:833657/251‑1 (MQ=255)                                                                                                                                          aaTTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGccc                                                                                                     <  1:672318/251‑1 (MQ=255)                                                                                                                                             tttGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCtt                                                                                                  <  1:337036/251‑1 (MQ=255)                                                                                                                                              ttGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTg                                                                                                                                                 <  1:494345/203‑1 (MQ=255)                                                                                                                                                   gacctgaccGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTggggg                                                                                             <  1:474579/250‑1 (MQ=255)                                                                                                                                                    acctgaccGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGAt                                                                                           <  1:94561/251‑1 (MQ=255)                                                                                                                                                         accGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGATAAATc                                                                                      <  1:518404/251‑1 (MQ=255)                                                                                                                                                                 cGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAg                                                                                                                                                   >  1:555199/1‑182 (MQ=255)                                                                                                                                                                   gCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCtgttgttg                                                                                                                                                       <  1:710422/176‑1 (MQ=255)                                                                                                                                                                   gCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGtt                                                                                                                          >  1:514650/1‑205 (MQ=255)                                                                                                                                                                   gCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGATAAATCCGCCGCcaca                                                                            >  1:850410/1‑251 (MQ=255)                                                                                                                                                                        gagCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGATAAATCCGCCGCCACAAGGc                                                                        <  1:350689/250‑1 (MQ=255)                                                                                                                                                                            ggCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGc                                                                                                            >  1:237171/1‑210 (MQ=255)                                                                                                                                                                                 aTGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGATAAATCCGCCGCCACAAGGc                                                                        <  1:865574/241‑1 (MQ=255)                                                                                                                                                                                                 aaCTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGATAAATCCGCCGCCACAAGGCAACGGCAAACCGACTTTTTCGCTTaa                                              >  1:715208/1‑251 (MQ=255)                                                                                                                                                                                                    tGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGATAAATCCGCCGCCACAAGGCAACGGCAAACCGACTTTTTCGCTTAATTc                                           <  1:492728/251‑1 (MQ=255)                                                                                                                                                                                                     gAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAg                                                                                                                                                   >  1:762924/1‑146 (MQ=255)                                                                                                                                                                                                        ggAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGATAAATCCGCCGCCACAAGGCAACGGCAAACCGACTTTTTCGCTTAATTCCg                                         <  1:115223/249‑1 (MQ=255)                                                                                                                                                                                                                   ccGTTCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGATAAATCCGCCGCCACAAGGCAACGGCAAACCGACTTTTTCGCTTAATTCCGCCTGCGTTAAGGc                            <  1:746594/251‑1 (MQ=255)                                                                                                                                                                                                                      ttCGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGATAAATCCGCCGCCACAAGGCAACGGCAAACCGACTTTTTCGCTTAATTCCGCCTGCGTTAAGGCTTg                         >  1:292090/1‑251 (MQ=255)                                                                                                                                                                                                                        cGCCAGCACCACAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTggg                                                                                               >  1:324117/1‑179 (MQ=255)                                                                                                                                                                                                                                ccacAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCtgttgttg                                                                                                                                                       <  1:56/115‑1 (MQ=255)                                                                                                                                                                                                                                ccacAATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGgcgc                                                                                                                <  1:566105/154‑1 (MQ=255)                                                                                                                                                                                                                                   caATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGATAAATCCGCCGCCACAAGGCAACGGCAAACCGACTTTTTCGCTTAATTCCGCCTGCGTTAAGGCTTGATAAAGATCGGTg            <  1:722481/251‑1 (MQ=255)                                                                                                                                                                                                                                   caATTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGATAAATCCGCCGCCACAAGGCAACGGCAAACCGACTTTTTCGCTTAATTCCGCCTGCGTTAAGGCTTGATAAAGATCGGTg            >  1:880673/1‑251 (MQ=255)                                                                                                                                                                                                                                     aTTTGATGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCTGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGATAAATcc                                                                                     >  1:260572/1‑176 (MQ=255)                                                                                                                                                                                                                                           tGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGATAAATCCGCCGCCACAAGGCAACGGCAAACCGACTTTTTCGCTTAATTCCGCCTGCGTTAAGGCTTGATAAAGATCGGTGGGCAAGTg    >  1:341940/1‑251 (MQ=255)                                                                                                                                                                                                                                           tGTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGATAAATCCGCCGCCACAAGGCAACGGCAAACCGACTTTTTCGCTTAATTCCGCCTGCGTTAAGGCTTGATAAAGATCGGTGGGCAAGTg    <  1:894977/251‑1 (MQ=255)                                                                                                                                                                                                                                            gTTGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGATAAATCCGCCGCCACAAGGCAACGGCAAACCGACTTTTTCGCTTAATTCCGCCTGCGTTAAGGCTTGATAAAGATCGGTGGGCAAGTGa   >  1:741553/1‑251 (MQ=255)                                                                                                                                                                                                                                             ttGAAAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGATAAATCCGCCGCCACAAGGCAACGGCAAACCGACTTTTTCGCTTAATTCCGCCTGCGTTAAGGCTTGATAAAGATCGGTGGGCAAGTGat  <  1:690581/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                        TCGCTAAAGCGACGATCGGCGCAATCGCGCACATGGCTGGCACCGATGCAATGGCTGGTTTTTGCTTGATCCGCAGGCGTGAGATAGCCGTCGTAGCAGAGCACGGATTTCAGTTTCAACAAATTTTCTGAGGTAGGAATTTTGCTGACCTGACCGCGCACGGCATAGAGCGGCAAATGCTGCGTTTGCGGGAACTGAGGGAGCTGATGTCCGTTCGCCAGCACCACAATTTGATGTTGAGAGCGTTCGCCTTGTTCATTGGTGAGCTGCCAGTGGTCGCCCAGCCATG‑AAAGTGCGGTCATTTTTTCCTGCGTTTTTAAGATCAAGCCCTGTTGTTGTAAGTGCGCAAAAAGATTTTGCACCAGTTGCTGTGGCGCAAGCCATGCCCCTTGGGGGATAAATCCGCCGCCACAAGGCAACGGCAAACCGACTTTTTCGCTTAATTCCGCCTGCGTTAAGGCTTGATAAAGATCGGTGGGCAAGTGAT  >  1/1613050‑1613536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑