Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,519,586 T→C D239D (GAT→GAC)  unknown → L‑seryl‑tRNA(Sec) selenium transferase (EC 2.9.1.1)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,519,5860TC100.0% 258.0 / NA 75D239D (GAT→GAC) unknownL‑seryl‑tRNA(Sec) selenium transferase (EC 2.9.1.1)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/32);  total (43/32)

CTGATGCTGGCGACCTTCGCCCAGGGCAAAGAAGTGATTATTTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGATGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCAAACG  >  1/1519341‑1519831
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
cTGATGCTGGCGACCTTCGCCCAGGGCAAAGAAGTGATTATTTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGTATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGc                                                                                                                                                                                                                                                   <  1:437089/250‑1 (MQ=255)
  gATGCTGGCGACCTTCGCCCAGGGCAAAGAAGTGATTATTTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAgg                                                                                                                                                                                                                                                >  1:745934/1‑251 (MQ=255)
             ccTTCGCCCAGGGCAAAGAAGTGATTATTTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGAt                                                                                                                                                                                                                                     >  1:342504/1‑251 (MQ=255)
                   cccAGGGCAAAGAAGTGATTATTTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTCTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGt                                                                                                                                                                                                                               <  1:438189/251‑1 (MQ=255)
                       gggCAAAGAAGTGATTATTTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAg                                                                                                                                                                                                                           >  1:6321/1‑251 (MQ=255)
                         gCAAAGAAGTGATTATTTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGaa                                                                                                                                                                                                                                              >  1:30277/1‑230 (MQ=255)
                         gCAAAGAAGTGATTATTTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGa                                                                                                                                                                                                                          <  1:332909/250‑1 (MQ=255)
                          cAAAGAAGTGATTATTTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGaa                                                                                                                                                                                                                         <  1:889191/250‑1 (MQ=255)
                               aagTGATTATTTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGc                                                                                                                                                                                                                   <  1:485950/251‑1 (MQ=255)
                                    attattTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTc                                                                                                                                                                                                               >  1:669549/1‑250 (MQ=255)
                                      tattTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCgg                                                                                                                                                                                                            <  1:552911/251‑1 (MQ=255)
                                           cccGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGtcat                                                                                                                                                                                                        >  1:131957/1‑250 (MQ=255)
                                                      cTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAg                                                                                                                                                                                             <  1:103727/250‑1 (MQ=255)
                                                       tGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGc                                                                                                                                                                                           <  1:257589/251‑1 (MQ=255)
                                                                 cggcggCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGgcgcgc                                                                                                                                                                                 <  1:700974/251‑1 (MQ=255)
                                                                          gTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGAtt                                                                                                                                                                        >  1:182351/1‑251 (MQ=255)
                                                                             ccGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTaa                                                                                                                                                                     >  1:176610/1‑251 (MQ=255)
                                                                                    cccGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAAtt                                                                                                                                                              >  1:584113/1‑251 (MQ=255)
                                                                                         catcatGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAg                                                                                                                                                                                                                                                 <  1:758320/163‑1 (MQ=255)
                                                                                           tcatGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTg                                                                                                                                                       >  1:588162/1‑251 (MQ=255)
                                                                                                gcgcAGGCGGGCTGCAAGCCGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGa                                                                                                                                                  >  1:143934/1‑251 (MQ=255)
                                                                                                     ggCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTGAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAagag                                                                                                                                              >  1:430713/1‑250 (MQ=255)
                                                                                                      gCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAagag                                                                                                                                              <  1:647276/249‑1 (MQ=255)
                                                                                                           cTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCg                                                                                                                                       >  1:153403/1‑251 (MQ=255)
                                                                                                                   tggtggAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGACTTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCCCCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGaa                                                                                                                               >  1:433157/1‑251 (MQ=255)
                                                                                                                    ggtggAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGc                                                                                                                                                                                           <  1:794390/190‑1 (MQ=255)
                                                                                                                    ggtggAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCg                                                                                                                                       >  1:281261/1‑242 (MQ=255)
                                                                                                                        gAAGTGGTCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAAtt                                                                                                                          >  1:795669/1‑251 (MQ=255)
                                                                                                                             gggCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAag                                                                                                                                                <  1:660863/224‑1 (MQ=255)
                                                                                                                             gggCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTgcag                                                                                                                      >  1:897096/1‑250 (MQ=255)
                                                                                                                             gggCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTgcag                                                                                                                      >  1:872625/1‑250 (MQ=255)
                                                                                                                                  cGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTgcagc                                                                                                                     >  1:114033/1‑246 (MQ=255)
                                                                                                                                      cAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGc                                                                                                                                    >  1:313740/1‑227 (MQ=255)
                                                                                                                                        aCCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGgat                                                                                                          >  1:798299/1‑251 (MQ=255)
                                                                                                                                                cATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGTTTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGgatttgattt                                                                                                   >  1:283112/1‑250 (MQ=255)
                                                                                                                                                cATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGgatttgattt                                                                                                   >  1:661700/1‑250 (MQ=255)
                                                                                                                                                 aTTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGc                                                                                                                                                                                           >  1:768930/1‑161 (MQ=255)
                                                                                                                                                 aTTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGc                                                                                                                                                                                           <  1:395231/161‑1 (MQ=255)
                                                                                                                                                 aTTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGAt                                                                                                                                                                         >  1:597091/1‑179 (MQ=255)
                                                                                                                                                              tCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGAt                                                                                                                                                                         >  1:349668/1‑166 (MQ=255)
                                                                                                                                                              tCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACa                                                                                    >  1:107979/1‑251 (MQ=255)
                                                                                                                                                                    cGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTg                                                                               >  1:654616/1‑250 (MQ=255)
                                                                                                                                                                      cAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTg                                                                               >  1:498778/1‑248 (MQ=255)
                                                                                                                                                                         ttAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGggc                                                                         <  1:280725/251‑1 (MQ=255)
                                                                                                                                                                         ttAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGggc                                                                         <  1:190528/251‑1 (MQ=255)
                                                                                                                                                                          tAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAg                                                                                                                                                                                                                           >  1:483129/1‑104 (MQ=255)
                                                                                                                                                                                   tACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAg                                                               <  1:255535/251‑1 (MQ=255)
                                                                                                                                                                                   tACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAg                                                               <  1:588020/251‑1 (MQ=255)
                                                                                                                                                                                        ccTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCg                                                             >  1:179141/1‑248 (MQ=255)
                                                                                                                                                                                              tAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCattatt                                                    >  1:249921/1‑251 (MQ=255)
                                                                                                                                                                                               aaTGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATtg                                                   <  1:430394/251‑1 (MQ=255)
                                                                                                                                                                                               aaTGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATtg                                                   <  1:463948/251‑1 (MQ=255)
                                                                                                                                                                                               aaTGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATtg                                                   <  1:605882/251‑1 (MQ=255)
                                                                                                                                                                                                    aaGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGc                                              <  1:12597/251‑1 (MQ=255)
                                                                                                                                                                                                           cagcagTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAg                                       >  1:815239/1‑251 (MQ=255)
                                                                                                                                                                                                             gcagTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGggc                                                                         >  1:197114/1‑215 (MQ=255)
                                                                                                                                                                                                             gcagTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCa                                     >  1:96480/1‑251 (MQ=255)
                                                                                                                                                                                                             gcagTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAACACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCa                                     >  1:454500/1‑251 (MQ=255)
                                                                                                                                                                                                                 tAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCAc                                    <  1:24257/248‑1 (MQ=255)
                                                                                                                                                                                                                    cTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTc                                                                                                                                                             >  1:180360/1‑124 (MQ=255)
                                                                                                                                                                                                                          aaTTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGc                        <  1:232542/251‑1 (MQ=255)
                                                                                                                                                                                                                          aaTTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGAGTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGc                        <  1:436911/251‑1 (MQ=255)
                                                                                                                                                                                                                                  gTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACaaa                                                                                  <  1:175353/185‑1 (MQ=255)
                                                                                                                                                                                                                                  gTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCAc                                    >  1:234941/1‑231 (MQ=255)
                                                                                                                                                                                                                                  gTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGc                 <  1:58779/250‑1 (MQ=255)
                                                                                                                                                                                                                                  gTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGCCGACAAATTGCCGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGc                 >  1:308048/1‑250 (MQ=255)
                                                                                                                                                                                                                                   ttttACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGcc                <  1:484562/250‑1 (MQ=255)
                                                                                                                                                                                                                                    tttACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCa                                 >  1:779604/1‑232 (MQ=255)
                                                                                                                                                                                                                                       aCCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCAccc           <  1:189057/251‑1 (MQ=255)
                                                                                                                                                                                                                                       aCCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCAccc           <  1:94879/251‑1 (MQ=255)
                                                                                                                                                                                                                                         ccACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCg          >  1:584575/1‑250 (MQ=255)
                                                                                                                                                                                                                                              gCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCaaa    >  1:901360/1‑251 (MQ=255)
                                                                                                                                                                                                                                               cGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCaaa    <  1:787227/250‑1 (MQ=255)
                                                                                                                                                                                                                                                 ttGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCAAAcg  >  1:49913/1‑250 (MQ=255)
                                                                                                                                                                                                                                                 ttGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCAAAcg  <  1:901835/250‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
CTGATGCTGGCGACCTTCGCCCAGGGCAAAGAAGTGATTATTTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGATGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCAAACG  >  1/1519341‑1519831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑