Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,456,806	C→A	V178V (GTC→GTA)	unknown →	ParA‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,456,806	0	C	A	100.0%	184.2 / NA	55	V178V (GTC→GTA)	unknown	ParA‑like protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (33/22);  total (33/22)

CTTGCCGATTACATTCTGATTCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCAATTGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCCGGGTTAGTCATCAGTCAAACGAATAAGAAGACAAATTTAGCTAAAGAAGTTATTTCGTTTATCAACGAAAACTTTGAAATACCGTTATTAAAGGGTAGTACGGCGGTACGCGTCAGCTATGCAGAAGTCTTAAGTTCCGGTAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGCACTAGGAGTTCAATATGAGTAAATTAACAAAAGGTACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAACGGCTATGCCACAGCTCAAAGAGGAAGTAACTACTGCCGAACAACGAACTACTATCATGATTCCGGTGGATATTTACAAAGC  >  1/1456564‑1457052                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       cTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAgctc                                                                                                                                                                                                                                                <  1:85244/251‑4 (MQ=255)                cTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGtttt                                                                                                                                                                                                                                 >  1:177639/1‑251 (MQ=255)                                  tCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAaaga                                                                                                                                                                                                               <  1:590249/251‑1 (MQ=255)                                  tCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAaaga                                                                                                                                                                                                               <  1:683301/251‑1 (MQ=255)                                       aTTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAAGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGa                                                                                                                                                                                                          >  1:425982/1‑251 (MQ=255)                                            atatTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGc                                                                                                                                                                                                      <  1:546383/250‑1 (MQ=255)                                            atatTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCa                                                                                                                                                                                                     >  1:404339/1‑251 (MQ=255)                                                            aGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAaaga                                                                                                                                                                                                               >  1:49132/1‑225 (MQ=255)                                                             gattcgatagcgCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTaatcaaatca                                                                                                                                                                                     >  1:740660/12‑250 (MQ=255)                                                             gATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAgc                                                                                                                                                                                                                                                  >  1:33978/1‑188 (MQ=255)                                                                 cgatagAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAaaga                                                                                                                                                                                                               <  1:242778/215‑1 (MQ=255)                                                                      gAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAAt                                                                                                                                                                           >  1:237924/1‑251 (MQ=255)                                                                           ggTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCg                                                                                                                                                                                                           <  1:662222/214‑1 (MQ=255)                                                                             tGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAAc                                                                                                                                                                     <  1:8772/250‑1 (MQ=255)                                                                                  gCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAgc                                                                                                                                                                                                                                                  <  1:620220/168‑2 (MQ=255)                                                                                   cACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTa                                                                                                                                                              <  1:525285/251‑1 (MQ=255)                                                                                     cGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAgg                                                                                                                                                            >  1:200031/1‑251 (MQ=255)                                                                                       tATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGa                                                                                                                                                           <  1:96834/250‑1 (MQ=255)                                                                                            aaaTTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCaa                                                                                                                                                     >  1:47557/1‑251 (MQ=255)                                                                                               ttAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAAGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAatat                                                                                                                                                  <  1:299934/251‑1 (MQ=255)                                                                                               ttAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTAGTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAatat                                                                                                                                                  <  1:430768/251‑1 (MQ=255)                                                                                                         gCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGa                                                                                                                                                           >  1:530502/1‑232 (MQ=255)                                                                                                                   ggtttAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAaaga                                                                                                                                                                                                               >  1:351420/4‑170 (MQ=255)                                                                                                                   ggtttAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAggca                                                                                                                              >  1:703895/4‑249 (MQ=255)                                                                                                                   ggtttAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAggca                                                                                                                              >  1:50307/4‑249 (MQ=255)                                                                                                                         gTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTaa                                                                                                                        >  1:674597/1‑251 (MQ=255)                                                                                                                                 tcaaacaaataaacaaaccaaTTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATcc                                                                                                                <  1:335871/232‑1 (MQ=255)                                                                                                                                      caaataaacaaaccaaTTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAgg                                                                                                                                                            >  1:471212/15‑202 (MQ=255)                                                                                                                                      caaataaacaaaccaaTTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCggg                                                                                                             >  1:334441/15‑249 (MQ=255)                                                                                                                                                               agatgttattgagTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAaaga                                                                                                                                                                                                               >  1:381207/13‑126 (MQ=255)                                                                                                                                                                 atgttattgagTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAg                                                                                                                                                          >  1:587947/11‑177 (MQ=255)                                                                                                                                                                 atgttattgagTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAAcag                                                                                 >  1:666918/11‑248 (MQ=255)                                                                                                                                                                 atgttattgagTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAAcag                                                                                 >  1:508724/11‑248 (MQ=255)                                                                                                                                                                 atgttattgagTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGGATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAAcagc                                                                                <  1:438021/241‑4 (MQ=255)                                                                                                                                                                   gttattgagTTTATTAAAGATAACTTTGAAATCCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAACAGCTa                                                                              >  1:551538/9‑251 (MQ=255)                                                                                                                                                                               attaaagataaCTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTaaa                                                                                                                                           >  1:517246/10‑178 (MQ=255)                                                                                                                                                                               attaaagataaCTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAACAGCTATGCCACAGCTCa                                                                  >  1:585959/10‑251 (MQ=255)                                                                                                                                                                                  aaagataaCTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAaaga                                                                                                                                                                                                               >  1:58835/7‑107 (MQ=255)                                                                                                                                                                                  aaagataaCTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCGGGCTTTCTATggg                                                                                                  >  1:653384/7‑216 (MQ=255)                                                                                                                                                                                   aagataaCTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAACAGCTATGCCACAGCTCAAaga                                                              <  1:334682/246‑1 (MQ=255)                                                                                                                                                                                          cTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAAc                                                                                   >  1:835765/1‑223 (MQ=255)                                                                                                                                                                                                         attagaaggcagtacaacggTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAACAGCTATGCCACAGCTCAAAGAGGAAGTAACTACTGCCGaacaa                                        >  1:774542/18‑251 (MQ=255)                                                                                                                                                                                                         attagaaggcagtacaacggTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAACAGCTATGCCACAGCTCAAAGAGGAAGTAACTACTGCCGaacaa                                        >  1:62875/18‑251 (MQ=255)                                                                                                                                                                                                                  cagtacaacggTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAACAGCTATGCCACAGCTCAAAGAGGAAGTAACTACTGCCGAACAACGAacta                                 >  1:656142/9‑249 (MQ=255)                                                                                                                                                                                                                    gtacaacggTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAaaga                                                                                                                                                                                                               <  1:238106/67‑1 (MQ=255)                                                                                                                                                                                                                    gtacaacggTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACt                                                                                                                          <  1:45997/152‑1 (MQ=255)                                                                                                                                                                                                                    gtacaacggTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAACAGCTATGCCACAGCTCAAAGAGGAAGTAACTACTGCCGaacaa                                        >  1:282902/7‑240 (MQ=255)                                                                                                                                                                                                                    gtacaacggTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAACAGCTATGCCACAGCTCAAAGAGGAAGTAACTACTGCCGAACAACGAACTACTAt                             >  1:164017/7‑251 (MQ=255)                                                                                                                                                                                                                      acaacggTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAACAGCTATGCCACAGCTCAAAGAGGAAGTAACTACTGCCGAACAACGAACTACTATCa                           <  1:168633/247‑1 (MQ=255)                                                                                                                                                                                                                               cgcgTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAACAGCTATGCCACAGCTCAAAGAGGAAGTAACTACTGCCGAACAACGAACTACTATCATGATTCCgg                  <  1:584263/251‑1 (MQ=255)                                                                                                                                                                                                                                      gCTATGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTaatcaaatc                                                                                                                                                                                      <  1:684989/80‑1 (MQ=255)                                                                                                                                                                                                                                         aTGCCGAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAACAGCTATGCCACAGCTCAAAGAGGAAGTAACTACTGCCGAACAACGAACTACTATCATGATTCCGGTGGATATTTa        >  1:252806/1‑251 (MQ=255)                                                                                                                                                                                                                                           gccgAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAatat                                                                                                                                                  >  1:433997/4‑111 (MQ=255)                                                                                                                                                                                                                                            ccgAAGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAACAGCTATGCCACAGCTCAAAGAGGAAGTAACTACTGCCGAACAACGAACTACTATCATGATTCCGGTGGATATTTAcc      <  1:260824/248‑2 (MQ=255)                                                                                                                                                                                                                                               aaGTATTAAGCTCCGGCAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGTACTAGGAGTTCAATATGAGTAAATTAACAAAAGGCACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAACAGCTATGCCACAGCTCAAAGAGGAAGTAACTACTGCCGAACAACGAACTACTATCATGATTCCGGTGGATATTTACAAagc  <  1:336131/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       CTTGCCGATTACATTCTGATTCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCAATTGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCCGGGTTAGTCATCAGTCAAACGAATAAGAAGACAAATTTAGCTAAAGAAGTTATTTCGTTTATCAACGAAAACTTTGAAATACCGTTATTAAAGGGTAGTACGGCGGTACGCGTCAGCTATGCAGAAGTCTTAAGTTCCGGTAATACGGTTTTTGAAAGTCAGTCTAAAGAAGCGAAAGCAGAGATTAATCAAATCACAAATGAAATTTTAACTGCACTAGGAGTTCAATATGAGTAAATTAACAAAAGGTACACTAAGCAAATCCGGGCTTTCTATGGGTAGCATTGAAAAAACGGCTATGCCACAGCTCAAAGAGGAAGTAACTACTGCCGAACAACGAACTACTATCATGATTCCGGTGGATATTTACAAAGC  >  1/1456564‑1457052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑