Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	278,004	G→A	A265V (GCG→GTG)	unknown ←	putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	278,004	0	G	A	100.0%	260.7 / NA	78	A265V (GCG→GTG)	unknown	putative membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (48/30);  total (48/30)

CAAATAATGCTGCCCCGCCACTTATCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCGCTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGATGCCCGCAACCGCAATTTTCGTGGTGTTCCGTATGCCCGTTAAAAACACCTGAAATTCTGACCGCACTTTTTCCTGCGATCAATGAACCG  >  1/277774‑278249                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      cAAATAATGCTGCCCCGCCACTTATCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATag                                                                                                                                                                                                                                   >  1:740472/1‑251 (MQ=255) cAAATAATGCTGCCCCGCCACTTATCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAACGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATag                                                                                                                                                                                                                                   >  1:436561/1‑251 (MQ=255)  aaaTAATGCTGCCCCGCCACTTATCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATaga                                                                                                                                                                                                                                  >  1:681662/1‑251 (MQ=255)   aataatGCTGCCCCGCCACTTATCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATagag                                                                                                                                                                                                                                 <  1:125437/251‑1 (MQ=255)    ataatGCTGCCCCGCCACTTATCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAgaga                                                                                                                                                                                                                                <  1:91784/251‑1 (MQ=255)    ataatGCTGCCCCGCCACTTATCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAgaga                                                                                                                                                                                                                                <  1:847172/251‑1 (MQ=255)    ataatGCTGCCCCGCCACTTATCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAgaga                                                                                                                                                                                                                                <  1:796815/251‑1 (MQ=255)    ataatGCTCCCCCGCCACTTATCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAgaga                                                                                                                                                                                                                                <  1:598081/251‑1 (MQ=255)       atGCTGCCCCGCCACTTATCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCa                                                                                                                                                                                                                                      <  1:289317/242‑1 (MQ=255)       atGCTGCCCCGCCACTTATCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATc                                                                                                                                                                                                                              >  1:501340/1‑250 (MQ=255)          ctgcCCCGCCACTTATCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCaa                                                                                                                                                                                                                           <  1:793948/250‑1 (MQ=255)            cccccGCCACTTATCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACat                                                                                                                                                                                                                        <  1:124455/250‑1 (MQ=255)               ccGCCACTTATCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACAtata                                                                                                                                                                                                                     >  1:245790/1‑251 (MQ=255)                  ccACTTATCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAgaga                                                                                                                                                                                                                                >  1:649656/1‑237 (MQ=255)                     cTTATCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATag                                                                                                                                                                                                                                   <  1:889838/231‑1 (MQ=255)                     cTTATCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAg                                                                                                                                                                                                               >  1:705575/1‑251 (MQ=255)                         tCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCCCATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAAc                                                                                                                                                                                                           <  1:709328/251‑1 (MQ=255)                         tCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAAc                                                                                                                                                                                                           <  1:570432/251‑1 (MQ=255)                         tCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAAc                                                                                                                                                                                                           <  1:540430/251‑1 (MQ=255)                            gCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGccc                                                                                                                                                                                                                                            <  1:811190/215‑1 (MQ=255)                               aTGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAgaga                                                                                                                                                                                                                                <  1:47143/224‑1 (MQ=255)                                   ggtgCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGaaaa                                                                                                                                                                                                 >  1:240147/1‑251 (MQ=255)                                   ggagCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGaaaa                                                                                                                                                                                                 >  1:63217/4‑251 (MQ=255)                                     tgCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAAt                                                                                                                                                                                                <  1:335162/250‑1 (MQ=255)                                      gCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATcg                                                                                                                                                                                              >  1:577704/1‑251 (MQ=255)                                           aaaGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGaa                                                                                                                                                                                                   <  1:649030/241‑1 (MQ=255)                                           aaaGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCg                                                                                                                                                                                         >  1:382022/1‑251 (MQ=255)                                           aaaGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCg                                                                                                                                                                                         >  1:56488/1‑251 (MQ=255)                                             aGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGa                                                                                                                                                                                       >  1:16298/1‑251 (MQ=255)                                               tAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAAc                                                                                                                                                                                     <  1:43056/251‑1 (MQ=255)                                                    tCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAgggg                                                                                                                                                                                <  1:103281/251‑1 (MQ=255)                                                              gAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGa                                                                                                                                                                      >  1:754417/1‑251 (MQ=255)                                                              gAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGa                                                                                                                                                                      >  1:67047/1‑251 (MQ=255)                                                                      atatGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACACCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCaaa                                                                                                                                                              >  1:438456/1‑251 (MQ=255)                                                                         tgatgCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAaata                                                                                                                                                           <  1:775540/251‑1 (MQ=255)                                                                           atgCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAaataat                                                                                                                                                         >  1:315/1‑251 (MQ=255)                                                                              ccGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCAt                                                                                                                                                                           >  1:205441/1‑230 (MQ=255)                                                                                           tCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTg                                                                                                                                         >  1:114752/1‑251 (MQ=255)                                                                                           tCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTg                                                                                                                                         >  1:696046/1‑251 (MQ=255)                                                                                            cATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCACCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGa                                                                                                                                        >  1:346842/1‑251 (MQ=255)                                                                                             aTTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGAt                                                                                                                                       >  1:865267/1‑251 (MQ=255)                                                                                                  ggCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAgaga                                                                                                                                                                                                                                <  1:870481/157‑1 (MQ=255)                                                                                                      tCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATAc                                                                                                                                                                   >  1:152705/1‑214 (MQ=255)                                                                                                      tCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTa                                                                                                                                >  1:389392/1‑249 (MQ=255)                                                                                                         gTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACatat                                                                                                                                                                                                                      >  1:420431/1‑160 (MQ=255)                                                                                                           aTTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTttgat                                                                                                                         <  1:87943/251‑1 (MQ=255)                                                                                                           aTTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTttgat                                                                                                                         >  1:628004/1‑251 (MQ=255)                                                                                                                    ggCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAGTCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGtt                                                                                                                >  1:450465/1‑251 (MQ=255)                                                                                                                          cGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTAc                                                                                                                               >  1:622722/1‑230 (MQ=255)                                                                                                                          cGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATCCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGt                                                                                                          <  1:445884/251‑1 (MQ=255)                                                                                                                               ggCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGcc                                                                                                                                                                                                                 <  1:433783/143‑1 (MQ=255)                                                                                                                               ggCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAgggg                                                                                                                                                                                >  1:588591/1‑176 (MQ=255)                                                                                                                                 cGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACatat                                                                                                                                                                                                                      >  1:717133/1‑136 (MQ=255)                                                                                                                                     aCCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGAAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGt                                                                                               <  1:770448/251‑1 (MQ=255)                                                                                                                                      ccACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGaa                                                                                                                                                                                                             >  1:687364/1‑140 (MQ=255)                                                                                                                                          tGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGATg                                                                                          <  1:305386/251‑1 (MQ=255)                                                                                                                                           gcgcCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGATg                                                                                          >  1:599195/1‑250 (MQ=255)                                                                                                                                                  tttCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACatat                                                                                                                                                                                                                      >  1:477403/1‑119 (MQ=255)                                                                                                                                                         gCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTttgat                                                                                                                         >  1:23243/1‑205 (MQ=255)                                                                                                                                                              ggATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAg                                                                                                                                                                                   <  1:716746/142‑1 (MQ=255)                                                                                                                                                              ggATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGATGCCCGCAACCGCAATTTTCgt                                                                      >  1:269294/1‑251 (MQ=255)                                                                                                                                                                    ggCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTAc                                                                                                                               >  1:568431/1‑188 (MQ=255)                                                                                                                                                                    ggCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGATGCCCGCAACCGCAAtt                                                                           >  1:176156/1‑240 (MQ=255)                                                                                                                                                                    ggCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGATGCCCGCAACCGCAATTTTCGTGGTGtt                                                                >  1:441858/1‑251 (MQ=255)                                                                                                                                                                      cATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGATGCCCGCAACCGCAATTTTCGTGGTGTTc                                                               <  1:751013/250‑1 (MQ=255)                                                                                                                                                                          gcgcACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGATGCCCGCAACCGCAATTTTCGTGGTGTTCCGTAt                                                          >  1:187905/1‑251 (MQ=255)                                                                                                                                                                               ccAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGGGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAAAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGATGCCCGCAACCGCAATTTTCGTGGTGTTCCGTATGcc                                                       <  1:837178/249‑1 (MQ=255)                                                                                                                                                                                              ccGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGGCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGATGCCCGCAACCGCAATTTTCGTGGTGTTCCGTATGCCCGTTAAAAACACCTGaa                                      >  1:352644/1‑251 (MQ=255)                                                                                                                                                                                                     tGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTAc                                                                                                                               >  1:680153/1‑155 (MQ=255)                                                                                                                                                                                                     tGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGAt                                                                                           >  1:384514/1‑191 (MQ=255)                                                                                                                                                                                                     tGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGATGcc                                                                                        >  1:420714/1‑194 (MQ=255)                                                                                                                                                                                                     tGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGATGCCCGCAACCGCAATTTTCGTGGTGTTCCGTATGCCCGTTAAAAACACCTGAAATTCTGa                               >  1:262922/1‑251 (MQ=255)                                                                                                                                                                                                          ttCCTGTGCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGATGCCCGCAACCGCAATTTTCGTGGTGTTCCGTATGCCCGTTAAAAACACCTGAAATTCTGACCGCa                          >  1:304448/1‑251 (MQ=255)                                                                                                                                                                                                                 gCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACat                                                                                                                                                                                                                        <  1:314767/54‑1 (MQ=255)                                                                                                                                                                                                                 gCCGAGAGCCATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGATGCCCGCAACCGCAATTTTCGTGGTGTTCCGTATGCCCGTTAAAAACACCTGAAATTCTGACCGCACttttt                    >  1:497009/1‑250 (MQ=255)                                                                                                                                                                                                                         ccATTGCCAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGATGCCCGCAACCGCAATTTTCGTGGTGTTCCGTAt                                                          >  1:618284/1‑204 (MQ=255)                                                                                                                                                                                                                                cAGTGTCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGATGCCCGCAACCGCAATTTTCGTGGTGTTCCGTa                                                           <  1:279349/196‑1 (MQ=255)                                                                                                                                                                                                                                  gtgtCACTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGATGCCCGCAACCGCAATTTTCGTGGTGTTCCGTATGCCCGTTAAAAACACCTGAAATTCTGACCGCACTTTTTCCTGCGATCAATGAACCg  >  1:35146/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      CAAATAATGCTGCCCCGCCACTTATCGGCAATGTGGTGCCATAAAGTAAACTCATCGCAAAGAAAATCAATATGATGCCGGCAAGGCATTTCATTAAGGCTTCCGTATTCACATTGGCAAACGGCAGGCGATACCACTGCGCCATTTTCACGGCAGTGGATCGGGCATAGCGCACCAACAACGCAAAGGCCGATAATGTCATTCCTGTGCCGAGAGCCATTGCCAGTGTCGCTATCACGCCCCAAGCATAGAGATCCAACATATACGCCAGAAACAGTACGAAAATCGCGCCGGAACAGGGGCGCATGCCGATACTCAAAATAATCAGCAAACTTTCTTTGATGTTGGTACTTTGATTGAGTCGTTCGGGGTTCGGCACATGTTGATGCCCGCAACCGCAATTTTCGTGGTGTTCCGTATGCCCGTTAAAAACACCTGAAATTCTGACCGCACTTTTTCCTGCGATCAATGAACCG  >  1/277774‑278249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑