Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,456,584	T→C	I104I (ATT→ATC)	unknown →	ParA‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,456,584	0	T	C	100.0%	247.8 / NA	71	I104I (ATT→ATC)	unknown	ParA‑like protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/41);  total (30/41)

CAGCTCAAAGATTTTGAAGTGGCATTAATTGACACTGACCCGCAAGCGTCCGCGCGTGAATGGAATGCGCTTGCCGGTGATGATTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTCTCCGAAAAAGAAATCCGTGCGCTTGGTCGCCAAGCGGATTTTTTAATCATTGATGGTGCGCCACGTATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATTCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCAATTGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCCGGGTTAGTCATCAGTCAAACGAATAAGAAGACAAATTTAGCTAAAGAAGTTATTTCGTTTATCAACGAAAACTTTGAAATACCGTTATTAAAGGGTAGTACGGCGGTACGCGTCAGCTATGCAGAAGTCTTAAGTTCCGGTAATACGGTTTT  >  1/1456351‑1456829                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      caacttaaaggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATcac                                                                                                                                                                                                                                       <  1:602088/238‑1 (MQ=255)          ggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGatat                                                                                                                                                                                                                             >  1:163389/4‑251 (MQ=255)          ggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGat                                                                                                                                                                                                                               <  1:269000/246‑1 (MQ=255)                 atgtcgCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGc                                                                                                                                                                                                                       <  1:241666/245‑1 (MQ=255)                       cATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCaaa                                                                                                                                                                                                                >  1:470649/1‑251 (MQ=255)                           aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGatat                                                                                                                                                                                                                             >  1:213797/1‑234 (MQ=255)                           aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGAtt                                                                                                                                                                                                            >  1:127422/1‑251 (MQ=255)                           aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGAtt                                                                                                                                                                                                            >  1:534074/1‑251 (MQ=255)                                     gACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTcgatagag                                                                                                                                                                                                    >  1:687922/1‑242 (MQ=255)                                     gACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTcgatagag                                                                                                                                                                                                    >  1:243854/1‑242 (MQ=255)                                      aCCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGa                                                                                                                                                                                                              >  1:126054/1‑238 (MQ=255)                                      aCCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTcgatagag                                                                                                                                                                                                    >  1:615720/1‑241 (MQ=255)                                         cacAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTg                                                                                                                                                                                              <  1:214139/249‑1 (MQ=255)                                         cacAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTg                                                                                                                                                                                              <  1:249534/249‑1 (MQ=255)                                          acAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTg                                                                                                                                                                                              >  1:285738/2‑250 (MQ=255)                                               cGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGc                                                                                                                                                                                         <  1:891707/250‑1 (MQ=255)                                               cGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCa                                                                                                                                                                                        <  1:832655/251‑1 (MQ=255)                                               cGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCa                                                                                                                                                                                        <  1:655757/251‑1 (MQ=255)                                               cGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCa                                                                                                                                                                                        <  1:716751/251‑1 (MQ=255)                                                   cgcgcgTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTa                                                                                                                                                                                    <  1:651967/251‑1 (MQ=255)                                                        gTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGaaa                                                                                                                                                                                                                                              >  1:117399/1‑188 (MQ=255)                                                         tgagtggaatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTg                                                                                                                                                                                              <  1:807745/231‑1 (MQ=255)                                                         tgagtggaatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCaaa                                                                                                                                                                              >  1:87197/5‑251 (MQ=255)                                                         tgagtggaatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCaaa                                                                                                                                                                              >  1:380005/5‑251 (MQ=255)                                                         tgagtggaatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGGGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCaa                                                                                                                                                                               <  1:429625/246‑1 (MQ=255)                                                               gaatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGCTCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATg                                                                                                                                                                        >  1:425015/1‑251 (MQ=255)                                                                aatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGa                                                                                                                                                                       <  1:225076/251‑1 (MQ=255)                                                                     gctcgCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGatat                                                                                                                                                                                                                             <  1:476719/188‑1 (MQ=255)                                                                           cGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAg                                                                                                                                                            <  1:336443/251‑1 (MQ=255)                                                                             gTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGc                                                                                                                                                           <  1:165671/250‑1 (MQ=255)                                                                                      ttttCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGtc                                                                                                                                                 <  1:583945/251‑1 (MQ=255)                                                                                              gtgtTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAAt                                                                                                                                                                                                                                    >  1:176844/1‑160 (MQ=255)                                                                                              gtgtTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGc                                                                                                                                                                                         <  1:289830/203‑1 (MQ=255)                                                                                              gtgtTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGtc                                                                                                                                                 <  1:761184/243‑1 (MQ=255)                                                                                                gtTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATcac                                                                                                                                                                                                                                       >  1:386318/1‑155 (MQ=255)                                                                                                gtTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTg                                                                                                                                                                                              <  1:329330/196‑1 (MQ=255)                                                                                                gtTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCaaa                                                                                                                                       >  1:890376/1‑251 (MQ=255)                                                                                                     cTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCaa                                                                                                                                                                               >  1:823734/1‑206 (MQ=255)                                                                                                        gtgATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAAc                                                                                                                                      <  1:320682/244‑1 (MQ=255)                                                                                                                  gctttcCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGTTTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATaaacaaaccaat                                                                                                                      <  1:259343/246‑11 (MQ=255)                                                                                                                            aaaGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATaaacaaacc                                                                                                                         >  1:813934/1‑230 (MQ=255)                                                                                                                                       ggtgcTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTAttgag                                                                                                 <  1:661241/247‑4 (MQ=255)                                                                                                                                        gtgcTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTAttgagtt                                                                                               >  1:508998/3‑246 (MQ=255)                                                                                                                                               gTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTAttaa                                                                                         <  1:461247/250‑4 (MQ=255)                                                                                                                                                            ttttttAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTAttgag                                                                                                 >  1:592423/1‑226 (MQ=255)                                                                                                                                                                       ttGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTAttgag                                                                                                 <  1:780512/218‑4 (MQ=255)                                                                                                                                                                           tggagctccccgcaTTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGttattagaag                                                            <  1:454616/238‑5 (MQ=255)                                                                                                                                                                             gagctccccgcaTTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACtt                                                                                >  1:487132/12‑229 (MQ=255)                                                                                                                                                                              agctccccgcaTTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGttattagaaggc                                                          <  1:756345/240‑7 (MQ=255)                                                                                                                                                                                 tccccgcaTTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGaaa                                                                           <  1:79786/223‑1 (MQ=255)                                                                                                                                                                                  ccccgcaTTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGttattagaaggcagtac                                                     <  1:423631/245‑12 (MQ=255)                                                                                                                                                                                  ccccgcaTTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGttattagaaggcagtac                                                     <  1:806558/245‑12 (MQ=255)                                                                                                                                                                                      gcaTTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTAttg                                                                                                   >  1:1276/3‑200 (MQ=255)                                                                                                                                                                                       caTTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGat                                                                                                                                                                                                                               <  1:206493/75‑1 (MQ=255)                                                                                                                                                                                           gAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAAc                                                                                                                                                                                                                                             <  1:858265/58‑1 (MQ=255)                                                                                                                                                                                                aGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTggg                                                                                                                                                                                                                        <  1:841488/74‑1 (MQ=255)                                                                                                                                                                                                aGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATaaac                                                                                                                              >  1:316435/1‑162 (MQ=255)                                                                                                                                                                                                 gCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAg                                      >  1:227459/1‑251 (MQ=255)                                                                                                                                                                                                    aTGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTa                                   <  1:480745/251‑1 (MQ=255)                                                                                                                                                                                                    aTGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTa                                   <  1:542422/251‑1 (MQ=255)                                                                                                                                                                                                     tGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGttattagaaggc                                                          >  1:675372/1‑221 (MQ=255)                                                                                                                                                                                                               gattAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCt                                    >  1:397924/1‑239 (MQ=255)                                                                                                                                                                                                                    aaCTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGttattagaaggcagtacaacgg                                                <  1:735347/222‑17 (MQ=255)                                                                                                                                                                                                                      cTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAgctc                 <  1:85244/251‑4 (MQ=255)                                                                                                                                                                                                                             aTTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTg                                                                                                                                                                                              >  1:407228/1‑71 (MQ=255)                                                                                                                                                                                                                             aTTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCa                                                                                                                                                                                            <  1:211094/73‑1 (MQ=255)                                                                                                                                                                                                                             aTTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTAt                                                                                                     >  1:127949/1‑160 (MQ=255)                                                                                                                                                                                                                                  aTTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGtc                                                                                                                                                 <  1:893915/111‑1 (MQ=255)                                                                                                                                                                                                                                  aTTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTAttaa                                                                                         <  1:573837/167‑4 (MQ=255)                                                                                                                                                                                                                                   ttCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATa                                                                                                                                                                     <  1:306975/90‑1 (MQ=255)                                                                                                                                                                                                                                     cTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATAAACAAACCAATTTAGCAAAAGATGTTATTGAGTTTATTAAAGATAACTTTGAAATTCCGTTATTAGAAGGCAGTACAACGGTTCGCGTCAGCTATGCCGAAGTATTAAGCTCCGGCAATACGGtttt  >  1:177639/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      CAGCTCAAAGATTTTGAAGTGGCATTAATTGACACTGACCCGCAAGCGTCCGCGCGTGAATGGAATGCGCTTGCCGGTGATGATTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTCTCCGAAAAAGAAATCCGTGCGCTTGGTCGCCAAGCGGATTTTTTAATCATTGATGGTGCGCCACGTATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATTCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCAATTGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCCGGGTTAGTCATCAGTCAAACGAATAAGAAGACAAATTTAGCTAAAGAAGTTATTTCGTTTATCAACGAAAACTTTGAAATACCGTTATTAAAGGGTAGTACGGCGGTACGCGTCAGCTATGCAGAAGTCTTAAGTTCCGGTAATACGGTTTT  >  1/1456351‑1456829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑