Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	270,359	A→G	A22A (GCA→GCG)	unknown →	N‑acetylneuraminate epimerase (EC 5.1.3.24)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	270,359	0	A	G	100.0%	244.6 / NA	72	A22A (GCA→GCG)	unknown	N‑acetylneuraminate epimerase (EC 5.1.3.24)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/30);  total (42/30)

ATTATTACCTGTGTGCCGAATTTATTAATACCTTAGGAAAAAAAGTGCGGTTGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCACAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTGGCGAACGCAACCAACCTGTCGCGGCGGCAGTTGATGGCAAATTGTACATTTTCGGCGGCTTACAAAAAAATGAAAAAGGC  >  1/270120‑270602                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    attattACCTGTGTGCCGAATTTATTAATACCTTAGGAAAAAAAGTGCGGTTGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTa                                                                                                                                                                                                                                          >  1:483736/1‑251 (MQ=255) attattACCTGTGTGCCGAATTTATTAATACCTTAGGAAAAAAAGTGCGGTTGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTa                                                                                                                                                                                                                                          >  1:273804/1‑251 (MQ=255)  ttattaCCTGTGTGCCGAATTTATTAATACCTTAGGAAAAAAAGTGCGGTTGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTAt                                                                                                                                                                                                                                         >  1:901197/1‑251 (MQ=255)   tattaCCTGTGTGCCGAATTTATTAATACCTTAGGAAAAAAAGTGCGGTTGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTAt                                                                                                                                                                                                                                         >  1:315098/1‑250 (MQ=255)         cTGTGTGCCGAATTTATTAATACCTTAGGAAAAAAAGTGCGGTTGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGAtt                                                                                                                                                                                                                                  <  1:222347/251‑1 (MQ=255)                ccGAATTTATTAATACCTTAGGAAAAAAAGTGCGGTTGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGt                                                                                                                                                                                                                           >  1:200204/1‑251 (MQ=255)                  gAATTTATTAATACCTTAGGAAAAAAAGTGCGGTTGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAACAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTgg                                                                                                                                                                                                                         >  1:455886/1‑251 (MQ=255)                       tattaATACCTTAGGAAAAAAAGTGCGGTTGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGAtt                                                                                                                                                                                                                                  <  1:169925/237‑1 (MQ=255)                       tattaATACCTTAGGAAAAAAAGTGCGGTTGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGAtt                                                                                                                                                                                                                    >  1:585186/1‑251 (MQ=255)                       tattaATACCTTAGGAAAAAAAGTGCGGTTGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTC‑AAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTa                                                                                                                                                                                                                   >  1:790316/1‑251 (MQ=255)                        attaATACCTTAGGAAAAAAAGTGCGGTTGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTa                                                                                                                                                                                                                   >  1:530312/1‑251 (MQ=255)                            aTACCTTAGGAAAAAAAGTGCGGTTGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAg                                                                                                                                                                                                               <  1:178206/251‑1 (MQ=255)                              aCCTTAGGAAAAAAAGTGCGGTTGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAgcg                                                                                                                                                                                                             >  1:836334/1‑251 (MQ=255)                               ccTTAGGAAAAAAAGTGCGGTTGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAagc                                                                                                                                                                                                                                                >  1:618072/1‑215 (MQ=255)                                     gAAAAAAAGTGCGGTTGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAgg                                                                                                                                                                                                      >  1:466573/1‑251 (MQ=255)                                                   ttGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAAc                                                                                                                                                                                         <  1:58241/250‑1 (MQ=255)                                                    tGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGa                                                                                                                                                                                       <  1:703157/251‑1 (MQ=255)                                                         aaCGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACgg                                                                                                                                                                                  <  1:321126/251‑1 (MQ=255)                                                         aaCGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACgg                                                                                                                                                                                  <  1:745560/251‑1 (MQ=255)                                                             cTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTa                                                                                                                                                                              >  1:733459/1‑251 (MQ=255)                                                                 aaaTTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTAtgtg                                                                                                                                                                          <  1:852855/251‑1 (MQ=255)                                                                 aaaTTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTAtgtg                                                                                                                                                                          >  1:546548/1‑251 (MQ=255)                                                                          ccGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGc                                                                                                                                                                 <  1:641110/251‑1 (MQ=255)                                                                          ccGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGc                                                                                                                                                                 <  1:301503/251‑1 (MQ=255)                                                                               cTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCggg                                                                                                                                                            >  1:526642/1‑251 (MQ=255)                                                                               cTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCggg                                                                                                                                                            >  1:405356/1‑251 (MQ=255)                                                                               cTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCggg                                                                                                                                                            >  1:738000/1‑251 (MQ=255)                                                                               cTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCggg                                                                                                                                                            >  1:773623/1‑251 (MQ=255)                                                                                tttAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGc                                                                                                                                                           <  1:807720/251‑1 (MQ=255)                                                                                     aCCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGgaga                                                                                                                                                      >  1:807733/1‑251 (MQ=255)                                                                                            ggTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAAt                                                                                                                                                 <  1:83447/249‑1 (MQ=255)                                                                                                              cTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAATAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAg                                                                                                                             <  1:444785/251‑1 (MQ=255)                                                                                                              cTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAg                                                                                                                             <  1:663930/251‑1 (MQ=255)                                                                                                              cTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAg                                                                                                                             <  1:141340/251‑1 (MQ=255)                                                                                                              cTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAg                                                                                                                             <  1:496063/251‑1 (MQ=255)                                                                                                                   acaAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGc                                                                                                                                                                 <  1:750086/210‑1 (MQ=255)                                                                                                                        aaaTCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGTCATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACg                                                                                                                   >  1:80497/1‑251 (MQ=255)                                                                                                                             tATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTAtgt                                                                                                                                                                           >  1:660607/1‑190 (MQ=255)                                                                                                                                     ccGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGgaaag                                                                                                       <  1:732089/250‑1 (MQ=255)                                                                                                                                        tttACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATAc                                                                                                                                                                                    <  1:747444/170‑1 (MQ=255)                                                                                                                                              aaTAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCcg                                                                                              <  1:28090/250‑1 (MQ=255)                                                                                                                                                  gCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCt                                                                                                                                                                <  1:830321/180‑1 (MQ=255)                                                                                                                                                  gCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGtt                                                                                          >  1:184987/1‑250 (MQ=255)                                                                                                                                                       cAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCggg                                                                                    >  1:616870/1‑251 (MQ=255)                                                                                                                                                       cAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCggg                                                                                    >  1:707302/1‑251 (MQ=255)                                                                                                                                                       cAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCggg                                                                                    >  1:72026/1‑251 (MQ=255)                                                                                                                                                       cAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCggg                                                                                    <  1:131366/251‑1 (MQ=255)                                                                                                                                                          tAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTgg                                                                                 <  1:7517/251‑1 (MQ=255)                                                                                                                                                             cTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGttt                                                                                                                                                                               >  1:439541/1‑154 (MQ=255)                                                                                                                                                             cTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTGGCGa                                                                              >  1:361855/1‑251 (MQ=255)                                                                                                                                                               cAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGATTTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTGGCGaa                                                                             >  1:880867/1‑250 (MQ=255)                                                                                                                                                                  aaCGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTGGCGAACGca                                                                         >  1:321053/1‑251 (MQ=255)                                                                                                                                                                       ggTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGTTACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGga                                                                                                          >  1:478187/1‑213 (MQ=255)                                                                                                                                                                        gtgtgtTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTAtg                                                                                                                                                                            >  1:191126/1‑146 (MQ=255)                                                                                                                                                                         tgtgtTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTGGCGAACGCaaccaacc                                                                  <  1:868560/251‑1 (MQ=255)                                                                                                                                                                          gtgtTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGaa                                                                                                                     <  1:901889/199‑1 (MQ=255)                                                                                                                                                                          gtgtTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTGGCGAACGcaaccaac                                                                   >  1:787136/1‑249 (MQ=255)                                                                                                                                                                          gtgtTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTGGCGAACGcaaccaac                                                                   >  1:511177/1‑249 (MQ=255)                                                                                                                                                                          gtgtTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTGGCGAACGCaaccaacc                                                                  >  1:322964/1‑250 (MQ=255)                                                                                                                                                                            gtTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATc                                                                                                                                                                                            <  1:555604/126‑1 (MQ=255)                                                                                                                                                                                            aaaCAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTGGCGAACGCAACCAACCTGTCGCGGCGGCAGTTGAt                                               >  1:403605/1‑251 (MQ=255)                                                                                                                                                                                                          aaTTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTGGCGAACGCAACCAACCTGTCGCGGCGGCAGTTGATGGCAAATTGTACAt                                 >  1:760003/1‑251 (MQ=255)                                                                                                                                                                                                             ttatttTTTGCTGCAACGGCTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTGGCGAACGCAACCAACCTGTCGCGGCGGCAGTTGATGGCAAATTGTACAtttt                              <  1:392617/251‑1 (MQ=255)                                                                                                                                                                                                                                cTTTTGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCg                                                                                                               <  1:220663/151‑1 (MQ=255)                                                                                                                                                                                                                                    tGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAAc                                                                                                                                                                                         >  1:818252/1‑73 (MQ=255)                                                                                                                                                                                                                                    tGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTGGCGAACGCAACCAACCTGTcgc                                                            >  1:481068/1‑198 (MQ=255)                                                                                                                                                                                                                                    tGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTGGCGAACGCAACCAACCTGTCGCGGCGGCAGTTGATGGCAAATTGTACATTTTCGGCGGCTTACAAAAAAATGaaa       >  1:736908/1‑251 (MQ=255)                                                                                                                                                                                                                                    tGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTGGCGAACGCAACCAACCTGTCGCGGCGGCAGTTGATGGCAAATTGTACATTTTCGGCGGCTTACAAAAAAATGaaa       >  1:315552/1‑251 (MQ=255)                                                                                                                                                                                                                                    tGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTGGCGAACGCAACCAACCTGTCGCGGCGGCAGTTGATGGCAAATTGTACATTTTCGGCGGCTTACAAAAAAATGaaa       >  1:281320/1‑251 (MQ=255)                                                                                                                                                                                                                                    tGCCACGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTGGCGAACGCAACCAACCTGTCGCGGCGGCAGTTGATGGCAAATTGTACATTTTCGGCGGCTTACAAAAAAATGaaa       >  1:192744/1‑251 (MQ=255)                                                                                                                                                                                                                                         cGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTAtgtg                                                                                                                                                                          <  1:310678/83‑1 (MQ=255)                                                                                                                                                                                                                                         cGACTGCGCAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTGGCGAACGCAACCAACCTGTCGCGGCGGCAGTTGATGGCAAATTGTACATTTTCGGCGGCTTACAAAAAAATGAAAAAGGc  <  1:875490/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    ATTATTACCTGTGTGCCGAATTTATTAATACCTTAGGAAAAAAAGTGCGGTTGAAAAACGCTGTAAATTTTAACCGCACTTTAAACCCTAGGGTGCAACCGGTTGCGCCCTACCACAAAAAATCTATTCATGCCGTTTACGAATAGCAAACAATAACTCAAAACGAGGTGTGTTATGACATTCAAAAAAACAACATTAGCGAATTTATTTTTTGCTGCAACGGCTTTTGCCACGACTGCACAAGCAGCTTATCCCGATTTACCCGTGGCGATTAAAAGCGGCGCAGGCGCGTTAATCAACGATACGGTTTATGTGGGCTTAGGCTCGGGCGGAGATAAATTTTATGCCTTGGATTTAAAGACCCCGAACGCCCGATGGAAAGAAATTGCCGCGTTCCCGGGTGGCGAACGCAACCAACCTGTCGCGGCGGCAGTTGATGGCAAATTGTACATTTTCGGCGGCTTACAAAAAAATGAAAAAGGC  >  1/270120‑270602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑