Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,456,485	T→G	R71R (CGT→AGG)	unknown →	ParA‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,456,485	0	T	G	98.5%	230.1 / ‑3.8	67	R71R (CGT→AGG)	unknown	ParA‑like protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (35/31);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TAACCGTTTCAAAGGAGACCCTTTATTATGAAAGTCATATCCGTTTTAAGTCAAAAAGGTGGTAGCGGTAAAAGTACGCTCTCAATTAATATCGCTCGTTGTTTGCAGCTCAAAGATTTTGAAGTGGCATTAATTGACACTGACCCGCAAGCGTCCGCGCGTGAATGGAATGCGCTTGCCGGTGATGATTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTCTCCGAAAAAGAAATCCGTGCGCTTGGTCGCCAAGCGGATTTTTTAATCATTGATGGTGCGCCACGTATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATTCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCAATTGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCCGGGTTAGTCATCAGTCAAACGAATAAGAAGACAAAT  >  1/1456246‑1456713                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                     ttacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCg                                                                                                                                                                                                                           >  1:485452/11‑251 (MQ=255)      gtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCagg                                                                                                                                                                                                                      >  1:418658/6‑249 (MQ=255)      gtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCagg                                                                                                                                                                                                                      >  1:217860/6‑249 (MQ=255)       tctttaaGGAGACCCTTTATTATGAAAGTCATATCCGTATTAAGTCAGAAAGGCGGTAGCGGTAAAAGCACGCTTTCTATCAATATTGCCCGCAGCCTGCAACTTAAAGGTTTTGATGTCGCATTAATTGATACCGACCCGCAAGCGTCCGCGCGTGAATGGAATGCGCTTGCCGGTGATGATTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTCTCCGAAAAAGAAATCCGTGCGCTTAGTCGCCAAg                                                                                                                                                                                                                      >  1:2884/6‑250 (MQ=255)        cttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCg                                                                                                                                                                                                                    >  1:585774/4‑251 (MQ=255)        cttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCg                                                                                                                                                                                                                    >  1:658698/4‑251 (MQ=255)                   cccTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAAt                                                                                                                                                                                                         >  1:544201/1‑251 (MQ=255)                                aaGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggag                                                                                                                                                                                              >  1:310955/1‑247 (MQ=255)                                 agttatatCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagc                                                                                                                                                                                             <  1:2741/245‑4 (MQ=255)                                  gttatatCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagctc                                                                                                                                                                                           >  1:583782/4‑245 (MQ=255)                                      tatctgtattAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagctccccgc                                                                                                                                                                                      <  1:533598/243‑11 (MQ=255)                                      tatctgtattAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagctccccg                                                                                                                                                                                       >  1:554259/9‑241 (MQ=255)                                        tctgtattAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagctccccgcat                                                                                                                                                                                    <  1:278310/245‑13 (MQ=255)                                                   ccagaaaGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCa                                                                                                                                                                         >  1:663622/5‑251 (MQ=255)                                                       aaaGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGAc                                                                                                                                                                     <  1:432086/251‑1 (MQ=255)                                                        aaGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGAcc                                                                                                                                                                    <  1:708465/251‑1 (MQ=255)                                                                                                       ctgcaacttaaaggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACt                                                                                                                                                      >  1:791679/16‑218 (MQ=255)                                                                                                          caacttaaaggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATcac                                                                                                                   <  1:602088/238‑1 (MQ=255)                                                                                                             cttaaaggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGAtc                                                                                                                                   >  1:19193/10‑230 (MQ=255)                                                                                                                   ggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGAc                                                                                                                                                                     <  1:293382/188‑1 (MQ=255)                                                                                                                   ggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGatat                                                                                                         >  1:163389/4‑251 (MQ=255)                                                                                                                   ggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGat                                                                                                           <  1:269000/246‑1 (MQ=255)                                                                                                                          atgtcgCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGc                                                                                                   <  1:241666/245‑1 (MQ=255)                                                                                                                                cATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCaaa                                                                                            >  1:470649/1‑251 (MQ=255)                                                                                                                                    aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTaa                                                                                                                                                                                                          <  1:766070/137‑1 (MQ=255)                                                                                                                                    aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAAc                                                                                                                                                       >  1:160304/1‑188 (MQ=255)                                                                                                                                    aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGAtc                                                                                                                                   <  1:347801/208‑2 (MQ=255)                                                                                                                                    aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGatat                                                                                                         >  1:213797/1‑234 (MQ=255)                                                                                                                                    aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGAtt                                                                                        >  1:534074/1‑251 (MQ=255)                                                                                                                                    aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGAtt                                                                                        >  1:127422/1‑251 (MQ=255)                                                                                                                                              gACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCg                                                                                                                                                 >  1:710526/1‑184 (MQ=255)                                                                                                                                              gACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTcgatagag                                                                                >  1:687922/1‑242 (MQ=255)                                                                                                                                              gACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTcgatagag                                                                                >  1:243854/1‑242 (MQ=255)                                                                                                                                               aCCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGa                                                                                          >  1:126054/1‑238 (MQ=255)                                                                                                                                               aCCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTcgatagag                                                                                >  1:615720/1‑241 (MQ=255)                                                                                                                                                  cacAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTg                                                                          <  1:214139/249‑1 (MQ=255)                                                                                                                                                  cacAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTg                                                                          <  1:249534/249‑1 (MQ=255)                                                                                                                                                   acAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTg                                                                          >  1:285738/2‑250 (MQ=255)                                                                                                                                                        cGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGc                                                                     <  1:891707/250‑1 (MQ=255)                                                                                                                                                        cGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCa                                                                    <  1:832655/251‑1 (MQ=255)                                                                                                                                                        cGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCa                                                                    <  1:655757/251‑1 (MQ=255)                                                                                                                                                        cGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCa                                                                    <  1:716751/251‑1 (MQ=255)                                                                                                                                                         gTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATgg                                                                                                                                                                                                <  1:497361/126‑1 (MQ=255)                                                                                                                                                            cgcgcgTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTa                                                                <  1:651967/251‑1 (MQ=255)                                                                                                                                                                 gTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGaaa                                                                                                                          >  1:117399/1‑188 (MQ=255)                                                                                                                                                                  tgagtggaatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTg                                                                          <  1:807745/231‑1 (MQ=255)                                                                                                                                                                  tgagtggaatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCaaa                                                          >  1:87197/5‑251 (MQ=255)                                                                                                                                                                  tgagtggaatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCaaa                                                          >  1:380005/5‑251 (MQ=255)                                                                                                                                                                  tgagtggaatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGGGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCaa                                                           <  1:429625/246‑1 (MQ=255)                                                                                                                                                                    agtggaatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTc                                                                                                                                        >  1:874230/3‑171 (MQ=255)                                                                                                                                                                        gaatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGCTCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATg                                                    >  1:425015/1‑251 (MQ=255)                                                                                                                                                                         aatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGa                                                   <  1:225076/251‑1 (MQ=255)                                                                                                                                                                              gctcgCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGatat                                                                                                         <  1:476719/188‑1 (MQ=255)                                                                                                                                                                                    cGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAg                                        <  1:336443/251‑1 (MQ=255)                                                                                                                                                                                      gTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGc                                       <  1:165671/250‑1 (MQ=255)                                                                                                                                                                                               ttttCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGtc                             <  1:583945/251‑1 (MQ=255)                                                                                                                                                                                                       gtgtTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAAt                                                                                                                >  1:176844/1‑160 (MQ=255)                                                                                                                                                                                                       gtgtTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGc                                                                     <  1:289830/203‑1 (MQ=255)                                                                                                                                                                                                       gtgtTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGtc                             <  1:761184/243‑1 (MQ=255)                                                                                                                                                                                                         gtTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATcac                                                                                                                   >  1:386318/1‑155 (MQ=255)                                                                                                                                                                                                         gtTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTg                                                                          <  1:329330/196‑1 (MQ=255)                                                                                                                                                                                                         gtTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCaaa                   >  1:890376/1‑251 (MQ=255)                                                                                                                                                                                                              cTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCaa                                                           >  1:823734/1‑206 (MQ=255)                                                                                                                                                                                                                 gtgATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGcc                                                                                                                                                  >  1:215311/1‑116 (MQ=255)                                                                                                                                                                                                                 gtgATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAAc                  <  1:320682/244‑1 (MQ=255)                                                                                                                                                                                                                           gctttcCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGTTTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATaaacaaaccaat  <  1:259343/246‑11 (MQ=255)                                                                                                                                                                                                                                     aaaGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCGGGTTTAGTCATCAGTCAAACAAATaaacaaacc     >  1:813934/1‑230 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                     TAACCGTTTCAAAGGAGACCCTTTATTATGAAAGTCATATCCGTTTTAAGTCAAAAAGGTGGTAGCGGTAAAAGTACGCTCTCAATTAATATCGCTCGTTGTTTGCAGCTCAAAGATTTTGAAGTGGCATTAATTGACACTGACCCGCAAGCGTCCGCGCGTGAATGGAATGCGCTTGCCGGTGATGATTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTCTCCGAAAAAGAAATCCGTGCGCTTGGTCGCCAAGCGGATTTTTTAATCATTGATGGTGCGCCACGTATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATTCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCAATTGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGCCGGGTTAGTCATCAGTCAAACGAATAAGAAGACAAAT  >  1/1456246‑1456713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑