Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,456,434	T→C	D54D (GAT→GAC)	unknown →	ParA‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,456,434	0	T	C	98.5%	212.7 / ‑4.1	65	D54D (GAT→GAC)	unknown	ParA‑like protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (35/29);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

GCGTTAACTCAATCTTAACCGTTTCAAAGGAGACCCTTTATTATGAAAGTCATATCCGTTTTAAGTCAAAAAGGTGGTAGCGGTAAAAGTACGCTCTCAATTAATATCGCTCGTTGTTTGCAGCTCAAAGATTTTGAAGTGGCATTAATTGACACTGACCCGCAAGCGTCCGCGCGTGAATGGAATGCGCTTGCCGGTGATGATTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTCTCCGAAAAAGAAATCCGTGCGCTTGGTCGCCAAGCGGATTTTTTAATCATTGATGGTGCGCCACGTATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATTCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCAATTGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGC  >  1/1456231‑1456676                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   ttgttacgcattgcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAAt                                                                                                                                                                                                     >  1:97588/26‑251 (MQ=255) ttgttacgcattgcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAAt                                                                                                                                                                                                     >  1:71488/26‑251 (MQ=255)  tgttacgcattgcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATc                                                                                                                                                                                                    <  1:737046/227‑1 (MQ=255)  tgttacgcattgcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATc                                                                                                                                                                                                    <  1:1692/227‑1 (MQ=255)   gttacgcattgcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATca                                                                                                                                                                                                   >  1:264620/24‑250 (MQ=255)           ttgcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATcaggg                                                                                                                                                                                                >  1:117857/16‑242 (MQ=255)             gcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATcagggtgcttgg                                                                                                                                                                                         >  1:701250/14‑240 (MQ=255)             gcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATcaggg                                                                                                                                                                                                <  1:460821/231‑5 (MQ=255)                ttacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCg                                                                                                                                                                                      >  1:485452/11‑251 (MQ=255)                     gtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCagg                                                                                                                                                                                 >  1:418658/6‑249 (MQ=255)                     gtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCagg                                                                                                                                                                                 >  1:217860/6‑249 (MQ=255)                      tctttaaGGAGACCCTTTATTATGAAAGTCATATCCGTATTAAGTCAGAAAGGCGGTAGCGGTAAAAGCACGCTTTCTATCAATATTGCCCGCAGCCTGCAACTTAAAGGTTTTGATGTCGCATTAATTGATACCGACCCGCAAGCGTCCGCGCGTGAATGGAATGCGCTTGCCGGTGATGATTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTCTCCGAAAAAGAAATCCGTGCGCTTAGTCGCCAAg                                                                                                                                                                                 >  1:2884/6‑250 (MQ=255)                       cttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCg                                                                                                                                                                               >  1:585774/4‑251 (MQ=255)                       cttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCg                                                                                                                                                                               >  1:658698/4‑251 (MQ=255)                                  cccTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAAt                                                                                                                                                                    >  1:544201/1‑251 (MQ=255)                                               aaGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggag                                                                                                                                                         >  1:310955/1‑247 (MQ=255)                                                agttatatCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagc                                                                                                                                                        <  1:2741/245‑4 (MQ=255)                                                 gttatatCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagctc                                                                                                                                                      >  1:583782/4‑245 (MQ=255)                                                     tatctgtattAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagctccccgc                                                                                                                                                 <  1:533598/243‑11 (MQ=255)                                                     tatctgtattAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagctccccg                                                                                                                                                  >  1:554259/9‑241 (MQ=255)                                                       tctgtattAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagctccccgcat                                                                                                                                               <  1:278310/245‑13 (MQ=255)                                                          gtattAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGc                                                                                                                                                                                                                     <  1:152677/175‑1 (MQ=255)                                                                  ccagaaaGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCa                                                                                                                                    >  1:663622/5‑251 (MQ=255)                                                                      aaaGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGAc                                                                                                                                <  1:432086/251‑1 (MQ=255)                                                                       aaGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGAcc                                                                                                                               <  1:708465/251‑1 (MQ=255)                                                                                                                      ctgcaacttaaaggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACt                                                                                                                 >  1:791679/16‑218 (MQ=255)                                                                                                                         caacttaaaggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATcac                                                                              <  1:602088/238‑1 (MQ=255)                                                                                                                            cttaaaggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGAtc                                                                                              >  1:19193/10‑230 (MQ=255)                                                                                                                                  ggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGAc                                                                                                                                <  1:293382/188‑1 (MQ=255)                                                                                                                                  ggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGatat                                                                    >  1:163389/4‑251 (MQ=255)                                                                                                                                  ggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGat                                                                      <  1:269000/246‑1 (MQ=255)                                                                                                                                         atgtcgCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGc                                                              <  1:241666/245‑1 (MQ=255)                                                                                                                                               cATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCaaa                                                       >  1:470649/1‑251 (MQ=255)                                                                                                                                                   aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAg                                                                                                                                                                                                                        >  1:696172/1‑86 (MQ=255)                                                                                                                                                   aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTaa                                                                                                                                                                     <  1:766070/137‑1 (MQ=255)                                                                                                                                                   aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAAc                                                                                                                  >  1:160304/1‑188 (MQ=255)                                                                                                                                                   aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGAtc                                                                                              <  1:347801/208‑2 (MQ=255)                                                                                                                                                   aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGatat                                                                    >  1:213797/1‑234 (MQ=255)                                                                                                                                                   aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGAtt                                                   >  1:127422/1‑251 (MQ=255)                                                                                                                                                   aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGAtt                                                   >  1:534074/1‑251 (MQ=255)                                                                                                                                                             gACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCg                                                                                                            >  1:710526/1‑184 (MQ=255)                                                                                                                                                             gACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTcgatagag                                           >  1:687922/1‑242 (MQ=255)                                                                                                                                                             gACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTcgatagag                                           >  1:243854/1‑242 (MQ=255)                                                                                                                                                              aCCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGa                                                     >  1:126054/1‑238 (MQ=255)                                                                                                                                                              aCCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTcgatagag                                           >  1:615720/1‑241 (MQ=255)                                                                                                                                                                 cacAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTg                                     <  1:249534/249‑1 (MQ=255)                                                                                                                                                                 cacAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTg                                     <  1:214139/249‑1 (MQ=255)                                                                                                                                                                  acAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTg                                     >  1:285738/2‑250 (MQ=255)                                                                                                                                                                       cGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGc                                <  1:891707/250‑1 (MQ=255)                                                                                                                                                                       cGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCa                               <  1:716751/251‑1 (MQ=255)                                                                                                                                                                       cGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCa                               <  1:832655/251‑1 (MQ=255)                                                                                                                                                                       cGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCa                               <  1:655757/251‑1 (MQ=255)                                                                                                                                                                        gTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATgg                                                                                                                                                           <  1:497361/126‑1 (MQ=255)                                                                                                                                                                           cgcgcgTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTa                           <  1:651967/251‑1 (MQ=255)                                                                                                                                                                                gTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGaaa                                                                                     >  1:117399/1‑188 (MQ=255)                                                                                                                                                                                 tgagtggaatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTg                                     <  1:807745/231‑1 (MQ=255)                                                                                                                                                                                 tgagtggaatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCaaa                     >  1:380005/5‑251 (MQ=255)                                                                                                                                                                                 tgagtggaatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCaaa                     >  1:87197/5‑251 (MQ=255)                                                                                                                                                                                 tgagtggaatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGGGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCaa                      <  1:429625/246‑1 (MQ=255)                                                                                                                                                                                   agtggaatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTc                                                                                                   >  1:874230/3‑171 (MQ=255)                                                                                                                                                                                       gaatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGCTCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATg               >  1:425015/1‑251 (MQ=255)                                                                                                                                                                                        aatgCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGa              <  1:225076/251‑1 (MQ=255)                                                                                                                                                                                             gctcgCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGatat                                                                    <  1:476719/188‑1 (MQ=255)                                                                                                                                                                                                   cGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAg   <  1:336443/251‑1 (MQ=255)                                                                                                                                                                                                     gTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCGATAGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGc  <  1:165671/250‑1 (MQ=255)                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   GCGTTAACTCAATCTTAACCGTTTCAAAGGAGACCCTTTATTATGAAAGTCATATCCGTTTTAAGTCAAAAAGGTGGTAGCGGTAAAAGTACGCTCTCAATTAATATCGCTCGTTGTTTGCAGCTCAAAGATTTTGAAGTGGCATTAATTGACACTGACCCGCAAGCGTCCGCGCGTGAATGGAATGCGCTTGCCGGTGATGATTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTCTCCGAAAAAGAAATCCGTGCGCTTGGTCGCCAAGCGGATTTTTTAATCATTGATGGTGCGCCACGTATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATTCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATTCAATTGAGCTGGTGCAAGCACGTATGCAAATTAATGATAAGCTCAAAGC  >  1/1456231‑1456676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑