Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,456,383	C→T	D37D (GAC→GAT)	unknown →	ParA‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,456,383	0	C	T	100.0%	133.8 / NA	40	D37D (GAC→GAT)	unknown	ParA‑like protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/15);  total (25/15)

GCGTTAACTCAATCTTAACCGTTTCAAAGGAGACCCTTTATTATGAAAGTCATATCCGTTTTAAGTCAAAAAGGTGGTAGCGGTAAAAGTACGCTCTCAATTAATATCGCTCGTTGTTTGCAGCTCAAAGATTTTGAAGTGGCATTAATTGACACTGACCCGCAAGCGTCCGCGCGTGAATGGAATGCGCTTGCCGGTGATGATTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTCTCCGAAAAAGAAATCCGTGCGCTTGGTCGCCAAGCGGATTTTTTAATCATTGATGGTGCGCCACGTATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATTCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATT  >  1/1456231‑1456627                                                                                                                                                         |                                                                                                                                                                                                                                                     ttgttacgcattgcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAAt                                                                                                                                                    >  1:97588/26‑251 (MQ=255) ttgttacgcattgcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAAt                                                                                                                                                    >  1:71488/26‑251 (MQ=255)  tgttacgcattgcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATc                                                                                                                                                   <  1:737046/227‑1 (MQ=255)  tgttacgcattgcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATc                                                                                                                                                   <  1:1692/227‑1 (MQ=255)   gttacgcattgcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATca                                                                                                                                                  >  1:264620/24‑250 (MQ=255)           ttgcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATcaggg                                                                                                                                               >  1:117857/16‑242 (MQ=255)             gcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATcagggtgcttgg                                                                                                                                        >  1:701250/14‑240 (MQ=255)             gcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATcaggg                                                                                                                                               <  1:460821/231‑5 (MQ=255)                ttacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCg                                                                                                                                     >  1:485452/11‑251 (MQ=255)                     gtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCagg                                                                                                                                >  1:217860/6‑249 (MQ=255)                     gtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCagg                                                                                                                                >  1:418658/6‑249 (MQ=255)                      tctttaaGGAGACCCTTTATTATGAAAGTCATATCCGTATTAAGTCAGAAAGGCGGTAGCGGTAAAAGCACGCTTTCTATCAATATTGCCCGCAGCCTGCAACTTAAAGGTTTTGATGTCGCATTAATTGATACCGACCCGCAAGCGTCCGCGCGTGAATGGAATGCGCTTGCCGGTGATGATTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTCTCCGAAAAAGAAATCCGTGCGCTTAGTCGCCAAg                                                                                                                                >  1:2884/6‑250 (MQ=255)                       cttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCg                                                                                                                              >  1:585774/4‑251 (MQ=255)                       cttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCg                                                                                                                              >  1:658698/4‑251 (MQ=255)                                  cccTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAAt                                                                                                                   >  1:544201/1‑251 (MQ=255)                                               aaGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggag                                                                                                        >  1:310955/1‑247 (MQ=255)                                                agttatatCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagc                                                                                                       <  1:2741/245‑4 (MQ=255)                                                 gttatatCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagctc                                                                                                     >  1:583782/4‑245 (MQ=255)                                                     tatctgtattAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagctccccgc                                                                                                <  1:533598/243‑11 (MQ=255)                                                     tatctgtattAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagctccccg                                                                                                 >  1:554259/9‑241 (MQ=255)                                                       tctgtattAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagctccccgcat                                                                                              <  1:278310/245‑13 (MQ=255)                                                          gtattAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGc                                                                                                                                                                    <  1:152677/175‑1 (MQ=255)                                                                  ccagaaaGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCa                                                                                   >  1:663622/5‑251 (MQ=255)                                                                      aaaGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGAc                                                                               <  1:432086/251‑1 (MQ=255)                                                                       aaGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGAcc                                                                              <  1:708465/251‑1 (MQ=255)                                                                                                                      ctgcaacttaaaggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACt                                                                >  1:791679/16‑218 (MQ=255)                                                                                                                         caacttaaaggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATcac                             <  1:602088/238‑1 (MQ=255)                                                                                                                            cttaaaggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGAtc                                             >  1:19193/10‑230 (MQ=255)                                                                                                                                  ggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGAc                                                                               <  1:293382/188‑1 (MQ=255)                                                                                                                                  ggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGatat                   >  1:163389/4‑251 (MQ=255)                                                                                                                                  ggctttGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGat                     <  1:269000/246‑1 (MQ=255)                                                                                                                                         atgtcgCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGc             <  1:241666/245‑1 (MQ=255)                                                                                                                                               cATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCaaa      >  1:470649/1‑251 (MQ=255)                                                                                                                                                   aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAg                                                                                                                                                                       >  1:696172/1‑86 (MQ=255)                                                                                                                                                   aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTaa                                                                                                                    <  1:766070/137‑1 (MQ=255)                                                                                                                                                   aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAAc                                                                 >  1:160304/1‑188 (MQ=255)                                                                                                                                                   aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGAtc                                             <  1:347801/208‑2 (MQ=255)                                                                                                                                                   aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGatat                   >  1:213797/1‑234 (MQ=255)                                                                                                                                                   aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGAtt  >  1:127422/1‑251 (MQ=255)                                                                                                                                                   aaTTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATCCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGAtt  >  1:534074/1‑251 (MQ=255)                                                                                                                                                         |                                                                                                                                                                                                                                                     GCGTTAACTCAATCTTAACCGTTTCAAAGGAGACCCTTTATTATGAAAGTCATATCCGTTTTAAGTCAAAAAGGTGGTAGCGGTAAAAGTACGCTCTCAATTAATATCGCTCGTTGTTTGCAGCTCAAAGATTTTGAAGTGGCATTAATTGACACTGACCCGCAAGCGTCCGCGCGTGAATGGAATGCGCTTGCCGGTGATGATTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTCTCCGAAAAAGAAATCCGTGCGCTTGGTCGCCAAGCGGATTTTTTAATCATTGATGGTGCGCCACGTATTGAAAAAGCCATGACCGATTCGATTAAACTTGCCGATTACATTCTGATTCCACTGAAACCATCACAATTTGATATTTGGGCATGCAAAGATT  >  1/1456231‑1456627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑