Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	269,498	A→G	Q398Q (CAA→CAG)	unknown →	TRAP‑type transport system, small permease component, predicted N‑acetylneuraminate transporter / TRAP‑type transport system, large permease component, predicted N‑acetylneuraminate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	269,498	0	A	G	100.0%	224.9 / NA	65	Q398Q (CAA→CAG)	unknown	TRAP‑type transport system, small permease component, predicted N‑acetylneuraminate transporter / TRAP‑type transport system, large permease component, predicted N‑acetylneuraminate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/33);  total (32/33)

ACGCAGGTTATGACGATGACGTTTGTGGTGGTATCACTGCGGCTTCCTGTATCATCGGCCCACTAGTGCCGCCAAGTATCGCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAACTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAACAGCTGTGTGGAAGCCATGGCAATTACCGGCGTGGTCGCCCTGATGATCATGACGGTAACTTTCTTCGG  >  1/269257‑269740                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   aCGCAGGTTATGACGATGACGTTTGTGGTGGTATCACTGCGGCTTCCTGTATCATCGGCCCACTAGTGCCGCCAAGTATCGCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTgcgc                                                                                                                                                                                                                                            <  1:562585/250‑1 (MQ=255) aCGCAGGTTATGACGATGACGTTTGTGGTGGTATCACTGCGGCTTCCTGTATCATCGGCCCACTAGTGCCGCCAAGTATCGCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTgcgc                                                                                                                                                                                                                                            <  1:437785/250‑1 (MQ=255)      ggTTATGACGATGACGTTTGTGGTGGTATCACTGCGGCTTCCTGTATCATCGGCCCACTAGTGCCGCCAAGTATCGCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCttt                                                                                                                                                                                                                                      >  1:105553/1‑251 (MQ=255)      ggTTATGACGATGACGTTTGTGGTGGTATCACTGCGGCTTCCTGTATCATCGGCCCACTAGTGCCGCCAAGTATCGCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCttt                                                                                                                                                                                                                                      >  1:57591/1‑251 (MQ=255)      ggTTATGACGATGACGTTTGTGGTGGTATCACTGCGGCTTCCTGTATCATCGGCCCACTAGTGCCGCCAAGTATCGCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCttt                                                                                                                                                                                                                                      >  1:104530/1‑251 (MQ=255)           tgacgatgacgTTTGTGGTGGTATCACTGCGGCTTCCTGTATCATCGGCCCACTAGTGCCGCCAAGTATCGCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAg                                                                                                                                                                                                                                 <  1:205257/251‑1 (MQ=255)             acgatgacgTTTGTGGTGGTATCACTGCGGCTTCCTGTATCATCGGCCCACTAGTGCCGCCAAGTATCGCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAgg                                                                                                                                                                                                                                <  1:760839/250‑1 (MQ=255)                                 aTCACTGCGGCTTCCTGTATCATCGGCCCACTAGTGCCGCCAAGTATCGCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAAc                                                                                                                                                                                                           <  1:886917/251‑1 (MQ=255)                                   cACTGCGGCTTCCTGTATCATCGGCCCACTAGTGCCGCCAAGTATCGCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTc                                                                                                                                                                                                                         <  1:408128/235‑1 (MQ=255)                                            ttCCTGTATCATCGGCCCACTAGTGCCGCCAAGTATCGCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGa                                                                                                                                                                                                >  1:604081/1‑251 (MQ=255)                                               cTGTATCATCGGCCCACTAGTGCCGCCAAGTATCGCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGAt                                                                                                                                                                                               <  1:241397/249‑1 (MQ=255)                                                          gCCCACTAGTGCCGCCAAGTATCGCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTAt                                                                                                                                                                                   >  1:731576/1‑250 (MQ=255)                                                           cccACTAGTGCCGCCAAGTATCGCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTAtct                                                                                                                                                                                 <  1:80570/251‑1 (MQ=255)                                                                       gccAAGTATCGCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCgg                                                                                                                                                                          <  1:741445/246‑1 (MQ=255)                                                                            gTATCGCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAg                                                                                                                                                                 >  1:474397/1‑250 (MQ=255)                                                                            gTATCGCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTAC‑TTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTc                                                                                                                                                                                                                         <  1:387999/193‑1 (MQ=255)                                                                                 gCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGa                                                                                                                                                            >  1:244390/1‑250 (MQ=255)                                                                                  ccATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACa                                                                                                                                                          >  1:798922/1‑251 (MQ=255)                                                                                     tGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGaa                                                                                                                                                       >  1:572341/1‑251 (MQ=255)                                                                                       aTCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAAt                                                                                                                                                      >  1:276086/1‑250 (MQ=255)                                                                                         cATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAAt                                                                                                                                                      >  1:806788/1‑248 (MQ=255)                                                                                                  cGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGCATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGtagtag                                                                                                                                          <  1:451040/251‑1 (MQ=255)                                                                                                   gTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTAt                                                                                                                                                                                            <  1:250486/200‑1 (MQ=255)                                                                                                     tATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCgg                                                                                                                                       <  1:317165/251‑1 (MQ=255)                                                                                                      aTCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCgg                                                                                                                                       >  1:69442/1‑250 (MQ=255)                                                                                                      aTCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCgg                                                                                                                                       >  1:330147/1‑250 (MQ=255)                                                                                                                         cAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATc                                                                                                                   <  1:382908/251‑1 (MQ=255)                                                                                                                             aCTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATc                                                                                                                   <  1:520602/247‑1 (MQ=255)                                                                                                                                atttattGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAAt                                                                                                            >  1:13107/1‑251 (MQ=255)                                                                                                                                atttattGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTAACGGTAAAt                                                                                                            >  1:47502/1‑251 (MQ=255)                                                                                                                                 tttattGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAAtt                                                                                                           <  1:123863/251‑1 (MQ=255)                                                                                                                                               cATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAAc                                                                                             <  1:13633/251‑1 (MQ=255)                                                                                                                                               cATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAAc                                                                                             <  1:205348/251‑1 (MQ=255)                                                                                                                                                 tCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACtt                                                                                           >  1:213625/1‑251 (MQ=255)                                                                                                                                                      ggTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTAccc                                                                                       <  1:475825/250‑1 (MQ=255)                                                                                                                                                         gtCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTaa                                                                                   >  1:88382/1‑251 (MQ=255)                                                                                                                                                           cTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAAc                                                                                             <  1:383641/239‑1 (MQ=255)                                                                                                                                                           cTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTaaaa                                                                                 >  1:806691/1‑251 (MQ=255)                                                                                                                                                                tCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGc                                                                              <  1:210258/249‑1 (MQ=255)                                                                                                                                                                             aaTGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAACAGCtgtg                                                               >  1:252042/1‑251 (MQ=255)                                                                                                                                                                             aaTGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAACAGCtgtg                                                               >  1:237956/1‑251 (MQ=255)                                                                                                                                                                                gggTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAACAGCTGTGTgg                                                            >  1:86007/1‑251 (MQ=255)                                                                                                                                                                                  gTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAAc                                                                                             <  1:286812/216‑1 (MQ=255)                                                                                                                                                                                   tATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAACAGCTGTGTGGAAg                                                         <  1:821840/251‑1 (MQ=255)                                                                                                                                                                                     tGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAACAGCTGTGTGGAAGcc                                                       <  1:663523/251‑1 (MQ=255)                                                                                                                                                                                       aaCTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAAc                                                                      <  1:57599/234‑1 (MQ=255)                                                                                                                                                                                         cTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATaaa                                                                                                 >  1:766387/1‑205 (MQ=255)                                                                                                                                                                                         cTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTaaaa                                                                                 >  1:388802/1‑221 (MQ=255)                                                                                                                                                                                                   gCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAACAGCtgtgt                                                              >  1:45802/1‑230 (MQ=255)                                                                                                                                                                                                                  tatCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTAt                                                                                                                               >  1:860894/1‑150 (MQ=255)                                                                                                                                                                                                                    tCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTg                                                                                                                                                                                                 >  1:299460/1‑82 (MQ=255)                                                                                                                                                                                                                    tCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCTGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAACAGCTGTGTgg                                                            <  1:260938/215‑1 (MQ=255)                                                                                                                                                                                                                    tCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGgtag                                                                                                                                             >  1:346752/1‑134 (MQ=255)                                                                                                                                                                                                                    tCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTc                                                                                                                             <  1:782011/150‑1 (MQ=255)                                                                                                                                                                                                                    tCCAAGAACATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAACAGCTGTGTgg                                                            >  1:888546/1‑215 (MQ=255)                                                                                                                                                                                                                            cATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAACAGCTGTGTGGAAGCCATGGCAATTAcc                                           <  1:136670/224‑1 (MQ=255)                                                                                                                                                                                                                            cATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAACAGCTGTGTGGAAGCCATGGCAATTACCGGCGTGGTCGCCCTGATGATCATGACg                >  1:692221/1‑251 (MQ=255)                                                                                                                                                                                                                            cATCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAACAGCTGTGTGGAAGCCATGGCAATTACCGGCGTGGTCGCCCTGATGATCATGACg                >  1:590652/1‑251 (MQ=255)                                                                                                                                                                                                                             aTCGAAAACCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAg                                                                                                <  1:602860/170‑1 (MQ=255)                                                                                                                                                                                                                                 aaaaCCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAACAGCTGTGTGGAAGCCATGGCAATTACCGGCGTGGTCGCCCTGATGATCATGACGGTAAc           <  1:12687/251‑1 (MQ=255)                                                                                                                                                                                                                                 aaaaCCACCCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAACAGCTGTGTGGAAGCCATGGCAATTACCGGCGTGGTCGCCCTGATGATCATGACGGTAAc           <  1:74505/251‑1 (MQ=255)                                                                                                                                                                                                                                     ccaccCGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAACAGCTGTGTGGAAGCCATGGCAATTACCGGCGTGGTCGCCCtgatga                        <  1:804742/234‑1 (MQ=255)                                                                                                                                                                                                                                        cccGGGAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCggtgg                                                                                                                                                                                      <  1:408472/73‑1 (MQ=255)                                                                                                                                                                                                                                           gggAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAACAGCTGTGTGGAAGCCATGGCAATTACCGGCGTGGTCGCCCTGATGATCATGACGGTAACTTTCTTCgg  >  1:604233/1‑250 (MQ=255)                                                                                                                                                                                                                                            ggAGCAGCTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAACAGCTGTGTGGAAGCCATGGCAATTACCGGCGTGGTCGCCCTGATGATCATGAc                 >  1:774961/1‑234 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   ACGCAGGTTATGACGATGACGTTTGTGGTGGTATCACTGCGGCTTCCTGTATCATCGGCCCACTAGTGCCGCCAAGTATCGCCATGATCATTTACGGCGTTATCGCCAACGAATCCATTGCAAAACTATTTATTGCCGGCTTCATCCCGGGTGTCTTAGTCACCATCGCACTAATGGGTATGAACTATTACATTGCTAAAAAACGCGGATATCCAAGAACATCGAAAACCACCCGGGAGCAACTTTGCGCCTCTTTTAAAGGGGCATTCTGGGCGATTTTAACCCCGTTATTGATTATCGGTGGTATCTTCTCCGGTTTATTCAGCCCGACAGAATCTGCGGTAGTAGCGGCCTTCTATTCCATCATTATCGGTAAATTCGTGTATAAAGAACTTACCCTTAAAATGCTGTTCAACAGCTGTGTGGAAGCCATGGCAATTACCGGCGTGGTCGCCCTGATGATCATGACGGTAACTTTCTTCGG  >  1/269257‑269740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑