Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,455,963	T→C	intergenic (‑102/‑310)	unknown ← / → unknown	hypothetical protein/ParA‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,455,963	0	T	C	100.0%	37.3 / NA	12	intergenic (‑102/‑310)	unknown/unknown	hypothetical protein/ParA‑like protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/8);  total (4/8)

TACTTCTTCATTGGATTTGACTTTAATTTCTCCGTTATCTAGCCGTGCAAAAAATAACGCTTCTTCGGAAAGCGTCACTTTTTGTGGTAGGTAGTCATCTTTACGATAGTTATTC‑TGTGTATTTAAGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAACACATAAAAATAAATATGTTTTTATGTGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGTTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTCTGTACATAACTCATATCCCAGCAAGGAGTACAAATTATGAATATTCATCATATCATTATCTCTATTCTTATC  >  1/1455730‑1456157                                                                                                                                                                                                                                           |                                                                                                                                                                                                   caCTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGCTAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTAtgcg                                                                                                                                                                                   >  1:441258/2‑249 (MQ=255)                                                    aaacaatacttcttCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCt                                                                                                                                <  1:737277/243‑1 (MQ=255)                                                                                                                   cttgcatATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAata                                                                                                                                                                             >  1:731009/7‑144 (MQ=255)                                                                                                                     tgcatATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGttggcacataac                                                                <  1:593174/247‑12 (MQ=255)                                                                                                                         tATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACttat                                                            >  1:592425/1‑248 (MQ=255)                                                                                                                                             tCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCGCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACaaa                                        <  1:434772/251‑1 (MQ=255)                                                                                                                                               cTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAAtc                                      <  1:895159/251‑2 (MQ=255)                                                                                                                                                                                    acaacaATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAATCATGCAACTTAATCATATCATTATCTCTCTTCTTATc  <  1:458486/250‑1 (MQ=255)                                                                                                                                                                                                   tattaACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCt                                                                                                                                >  1:858519/1‑109 (MQ=255)                                                                                                                                                                                                        aCATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTc                                                                                       <  1:371622/145‑1 (MQ=255)                                                                                                                                                                                                                          gATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGttggcacataac                                                                <  1:159326/150‑12 (MQ=255)                                                                                                                                                                                                                          gATTAAGCATATAAAAACAAATATGTTTTTATGCGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGCTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTTGGCACATAACTTATATCCCAGCAAGGAGTACAAAtc                                      <  1:766106/176‑2 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                   TACTTCTTCATTGGATTTGACTTTAATTTCTCCGTTATCTAGCCGTGCAAAAAATAACGCTTCTTCGGAAAGCGTCACTTTTTGTGGTAGGTAGTCATCTTTACGATAGTTATTC‑TGTGTATTTAAGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAACACATAAAAATAAATATGTTTTTATGTGTAAATATTTATCAATATTTATTGATTTATGTTTTTATGTTTTGTATAATCTTTCTCGTGGTAACACAAAAAAAGAAACCTCTGATAGCGGTCACTATCAGAGGTCTGTACATAACTCATATCCCAGCAAGGAGTACAAATTATGAATATTCATCATATCATTATCTCTATTCTTATC  >  1/1455730‑1456157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑