Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	266,926	T→C	N30D (AAT→GAT)	unknown ←	N‑acetylmannosamine‑6‑phosphate 2‑epimerase (EC 5.1.3.9)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	266,926	0	T	C	100.0%	215.0 / NA	61	N30D (AAT→GAT)	unknown	N‑acetylmannosamine‑6‑phosphate 2‑epimerase (EC 5.1.3.9)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/35);  total (26/35)

CACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATTATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTAC  >  1/266690‑267133                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                cACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGa                                                                                                                                                                                                    >  1:688812/1‑250 (MQ=255)     gCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAaga                                                                                                                                                                                               >  1:109771/1‑251 (MQ=255)     gCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAaga                                                                                                                                                                                               >  1:508371/1‑251 (MQ=255)     gCGATAATGCCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑CCCGAAAAAAACGGGGTGATACGTACCGGGCTGTGGGG‑AAGACCCCGTTTGACGATACCGATGATCGGCACATTGACGGTCGGG‑CGCACGGTTTTTAAA‑TTGCCCCCCCCTCCAA‑TGCGCAACCCCCCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAag                                                                                                                                                                                                <  1:152315/250‑1 (MQ=255)       gATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAgg                                                                                                                                                                                             >  1:576867/1‑251 (MQ=255)        aTAATGCCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGAAAAAACGGGGTGATACGTCCGGGGCTGTCGGG‑AAGACCCCTTTTGACGATACCGATGATCGCCACATTTACGGTCGGG‑CGCACGTCTTTTAAA‑TTGTCCACCCCTTCAA‑GGCGCACCCCCCCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAgg                                                                                                                                                                                             <  1:548378/250‑1 (MQ=255)         acgggcccgcccgggCGTGCCCAAATTCTTCAATT‑CCCTGAAAAAGCGGGGTGATGCGTACCGGGCTGTCGGA‑AAGACCCCTTTTGACAACCCCGATGTTCGGCACATTTACGGCCGGG‑CCCACGGTTTTTAAT‑TTGCCCACCCCTACAA‑TGCCCACCCCCGCCCCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAgg                                                                                                                                                                                             <  1:150409/236‑1 (MQ=255)                      ggcgggcgCCAAGTCTTAATTG‑TCCTGAAAAAACGGGGGGATACGCACCGGGCTG‑CGGGAAAGATCCCGTTTGCCGATACGGAGGATCGCCACATTTCCGGTCGGG‑CGCACGGTTTTTAAA‑TTGTCCCCCCCTCAAA‑TGCGCACCCCCCCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTc                                                                                                                                                                               <  1:257526/250‑1 (MQ=255)                           ccgcCAAGTC‑TCAATGTTCATGAAAAAGCGGGTTGATACATCCGGGGCTGGCGGA‑AAAACCCCTTTGGAGGATACCGATGA‑CGGCAATTTTACCGTCGGGCCGCA‑GCCTTTTTAA‑TTTCCCCCCCCTTAAATTGCGC‑ACCCCGCCCCCCCTGCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAg                                                                                                                                                                          <  1:113418/249‑1 (MQ=255)                                 aGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCg                                                                                                                                                                   >  1:539883/1‑251 (MQ=255)                                  gTCTTAAATG‑TCCTGAAAAAGGGGGGTGATACGTACGGGGCTGTCGGG‑AAGATCCCGTTTGACGATCCCGATGATCGGCACATTTACGGGGGGG‑CGCACGGTTTTTAAA‑TTTTCCCCCCCTTCAA‑GGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCAc                                                                                                                                                                                                           <  1:277529/210‑1 (MQ=255)                                      tCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGaaaa                                                                                                                                                               >  1:451254/1‑250 (MQ=255)                                           g‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCtt                                                                                                                                                         >  1:658673/1‑251 (MQ=255)                                               cTGTAAAAGCGGGGTGATACGTCCGGGGCTGTGGGG‑AAGATCCCGTTTGACGATACGGATGATCGGCACATTTACGGGCGGG‑CGCGCGGTTTTTAAA‑TTGTCCACACCTTCAG‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTtgat                                                                                                                                                      <  1:91346/251‑1 (MQ=255)                                                 gTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTtgatga                                                                                                                                                    >  1:239835/1‑251 (MQ=255)                                                    aaacgggggTGATCCTTCCCGGGCTGTGGGG‑AATACCCCTTTTGCCGATACCAATGATCGCCACATTTCCGGTCGGG‑CGCCCGGCTTTTAAT‑TTGCCCACCCCTTCAA‑TGCGCACCCCCGCCCCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCAc                                                                                                                                                                                                           <  1:675361/188‑1 (MQ=255)                                                            gTGATCCGTCCCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACCCCTTCAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCatcatc                                                                                                                                                                                                              <  1:331252/182‑1 (MQ=255)                                                              gATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATCCCGATGATCGGCACATTTCCGGTCGGG‑CGCACGGTTTTTAAA‑TTGTCCACCCCTTCAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAAc                                                                                                                                                              <  1:21594/228‑1 (MQ=255)                                                              gATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTgag                                                                                                                                       >  1:18091/1‑251 (MQ=255)                                                              gATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTgag                                                                                                                                       >  1:474853/1‑251 (MQ=255)                                                              aaTACGTACGGGTCTGTCGGG‑AATATCCCGTTTGACATTACCGATGATCGGAACATTCGCGGTCGGG‑CGCATGGTTTTTAAT‑TTGTCCCCACTTACAT‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTgag                                                                                                                                       <  1:35786/250‑1 (MQ=255)                                                               atccGTACCGGGCTGTCGGA‑AAGTCCCCTTTTGACGATCCCGATGACCGCCACATTTCCGGTCGGG‑C‑CCCCGCTTTTAAATTTGCCCCCCCCTCCAA‑TCCCCACCCCCCCCCCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTgag                                                                                                                                       <  1:897889/247‑1 (MQ=255)                                                                   gtcccGGGCTGTCGGG‑AAGACCCCGTTTGACGATCCCGATGATCGGCACATTTACGGTCGGG‑CGCCCGGTTTTTAAA‑TTGTCCACCCCTTCAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGc                                                                                                                                                                                                       <  1:638053/179‑1 (MQ=255)                                                                   gTACGGGGCTGTGGGG‑AAGTCCCCGTTTGACGATCCCGATGATCGGCACATTTACGGCCGGG‑CGCACGGTTTTTAAT‑TTGTCCACCCCTTAAA‑GGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTaa                                                                                                                                                                                      <  1:492870/199‑1 (MQ=255)                                                                   gTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTGAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAg                                                                                                                                   >  1:271564/1‑250 (MQ=255)                                                                   gTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAg                                                                                                                                   >  1:413553/1‑250 (MQ=255)                                                                     ccggggCTGTGGGG‑AAGACCCCTTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGTTTTTAAA‑TTGTCCACCCCTTCAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCtg                                                                                                                                <  1:695615/248‑1 (MQ=255)                                                                             gTCGGG‑AAGATCCCGTTTGACGATCCCGATGATCGGCACATTTACGGTCGGG‑CGCACGGTTTTAAAA‑TTGTCCACCCCTTCAA‑GGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAg                                                                                                                                                                          <  1:690844/201‑1 (MQ=255)                                                                             gTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACa                                                                                                                           >  1:141668/1‑248 (MQ=255)                                                                             gTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAg                                                                                                                        >  1:383009/1‑251 (MQ=255)                                                                                  g‑AAGATCCTGTTTGACGATCCCGATGATCGGCACTTTTACGGGCGGG‑CGCGCGGTTTTTAAA‑TTGCCCACACCTTCAA‑TGCGCACCCCCGCCCCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAAc                                                                                                                    <  1:790854/250‑1 (MQ=255)                                                                                    aaGATCCCGTTTGACGATCCCGATGCTCGGCACATTTACGGTGGGG‑CGCACGTTTTTTAAA‑TGGCCCACACCTTCAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAActc                                                                                                                  <  1:632455/251‑1 (MQ=255)                                                                                       accccGTTTGACGATCCCGATGATCGCCCCATTTACGGGCGGG‑CGCACGGCTTTTAAA‑TTGCCCCCCCCTTCAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAActcc                                                                                                                 <  1:237467/247‑1 (MQ=255)                                                                                              ttGACGATCCCGATGATCGGCACATTTGCGGTCGGG‑CGCACGGTTTTTAAA‑TTGTCCACCCCTTCAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTg                                                                                                        <  1:8468/251‑1 (MQ=255)                                                                                                gACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTgag                                                                                                                                       >  1:683747/1‑218 (MQ=255)                                                                                                     tACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTg                                                                                                  >  1:247757/1‑250 (MQ=255)                                                                                                            gatCGGCACATTTCCGGTCGGG‑CGCCCGGTTTTAAAA‑TTGTCCCCCCCTTCAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGaaa                                                                                                                     <  1:163451/224‑1 (MQ=255)                                                                                                             atCGGCACATTTACGGTCGGG‑CGCCCGGTTTTTAAA‑TTGCCCCCCCCTTCAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGc                                                                                          <  1:146362/250‑1 (MQ=255)                                                                                                              tgggCACATTTACGGTGGGC‑CGCCCGGCTTTTAAT‑TTGCCCCCCCCTTCAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAg                                                                                        <  1:141963/249‑1 (MQ=255)                                                                                                                    caTTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCAtt                                                                                   >  1:25475/1‑250 (MQ=255)                                                                                                                     aTTTACGGTCGGG‑CGCACGGCTTTTAAT‑TTGCCCCCCCCTTCAA‑GCCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCtg                                                                                                                                <  1:318948/204‑1 (MQ=255)                                                                                                                     aTTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGCAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCAtt                                                                                   >  1:428334/1‑249 (MQ=255)                                                                                                                     aTTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCAtt                                                                                   >  1:23156/1‑249 (MQ=255)                                                                                                                         ccGGTCGGG‑CGCACGTTTTTAAAA‑TTGCCCCCCCCTTCAA‑GCCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTc                                                                             <  1:786831/250‑1 (MQ=255)                                                                                                                          cGGTGGGG‑CGCACGGCTTTAAAA‑TTGCCCACACCTTCAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTc                                                                             <  1:146642/250‑1 (MQ=255)                                                                                                                               ggg‑CGCACGGTTTTTAAA‑TTGCCCCCCCCTCCAA‑GGCGCACCCCCCCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTgg                                                                        <  1:408515/250‑1 (MQ=255)                                                                                                                                    ccACGTCTTTAAAA‑TTGCCCCCCCCTCCAA‑GGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACa                                                                   <  1:386460/250‑1 (MQ=255)                                                                                                                                       cgctttttAAA‑TTGCCCACCCCTCCAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTc                                                                <  1:265338/247‑1 (MQ=255)                                                                                                                                         gCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACa                                                                   >  1:868139/1‑246 (MQ=255)                                                                                                                                                     gccccccccTTCAA‑TCCGCACCCCCCCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGt                                                                                                                                                                         <  1:113638/126‑1 (MQ=255)                                                                                                                                                     gTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACa                                                                   >  1:134311/1‑235 (MQ=255)                                                                                                                                                      gcccacacctcaaaggcccacccccgccgcccc‑gccgATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACa                                                                   <  1:216808/214‑1 (MQ=255)                                                                                                                                                            ccTTAAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGc                                                                                          <  1:409482/205‑1 (MQ=255)                                                                                                                                                              ctccaatgcccccccccgccgcccc‑gccgATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACTATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCAttt                                           <  1:435298/238‑1 (MQ=255)                                                                                                                                                                                              aaCGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAAc                                                                                                                    >  1:360339/1‑147 (MQ=255)                                                                                                                                                                                               aCGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCttt                                                                                                            >  1:562523/1‑154 (MQ=255)                                                                                                                                                                                               aCGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTc                                                                             >  1:521310/1‑185 (MQ=255)                                                                                                                                                                                               aCGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTAt           >  1:209759/1‑251 (MQ=255)                                                                                                                                                                                                        tGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTAc  <  1:45375/251‑1 (MQ=255)                                                                                                                                                                                                        tGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTAc  <  1:387401/251‑1 (MQ=255)                                                                                                                                                                                                          cgcCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACa                                                                                                                           >  1:36643/1‑128 (MQ=255)                                                                                                                                                                                                                       aCAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACa                                                                   <  1:544093/171‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                CACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CGCACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATTATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTAC  >  1/266690‑267133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑