Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,455,771	G→A	L31L (CTA→TTA)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,455,771	0	G	A	100.0%	110.5 / NA	34	L31L (CTA→TTA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/17);  total (17/17)

ATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAGCGAAAAGAAACTTCCCTGCTTTGACTATCATACAAAATAAAACGAGCTTTTTCTAAAAGCTGTTGTTCCGCTTCTTCCATCCAAACTACGGTGTAATCCAATTTCATTTTAAGCCCAATACTTTTTCCAGGTTAGCTAATACTTCTTCATTGGATTTGACTTTAATTTCTCCGTTATCTAGCCGTGCAAAAAATAACGCTTCTTCGGAAAGCGTCACTTTTTGTGGTAGGTAGTCATCTTTACGATAGTTATTC‑TGTGTATTTAAGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAACACATAAAAATAAATATGTTTTTATGTG  >  1/1455530‑1455980                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                   atTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCg                                                                                                                                                                                                                 >  1:362266/1‑245 (MQ=255) atTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCg                                                                                                                                                                                                                 >  1:500174/1‑245 (MQ=255) atTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCaaa                                                                                                                                                                                                           >  1:313485/1‑251 (MQ=255)  ttATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCg                                                                                                                                                                                                                 <  1:392137/244‑1 (MQ=255)           gTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCaaaaaacaatac                                                                                                                                                                                                  <  1:727580/250‑7 (MQ=255)           gTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCaaaaaacaatac                                                                                                                                                                                                  <  1:506439/250‑7 (MQ=255)               cTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCaaaaaacaatacttctt                                                                                                                                                                                             >  1:897615/1‑240 (MQ=255)                 aCATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCg                                                                                                                                                                                                                 <  1:179950/229‑1 (MQ=255)                   aTAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCg                                                                                                                                                                                                                 <  1:734928/227‑1 (MQ=255)                    tAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGaa                                                                                                                                                                                        <  1:726771/251‑1 (MQ=255)                      ggATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAg                                                                                                                                                                                      >  1:413180/1‑251 (MQ=255)                      ggATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAg                                                                                                                                                                                      >  1:715473/1‑251 (MQ=255)                              tttGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACtt                                                                                                                                                                              <  1:699369/251‑1 (MQ=255)                                                                                                                   tAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGGCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATc                                                                                        <  1:277438/251‑1 (MQ=255)                                                                                                                         ctgctgctCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCa                                                                                             >  1:473074/8‑240 (MQ=255)                                                                                                                                tCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCg                                                                           <  1:859939/251‑1 (MQ=255)                                                                                                                                  cGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATc                                                                                        >  1:889129/1‑236 (MQ=255)                                                                                                                                          ccgtccaAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAg                                                                 <  1:500328/248‑1 (MQ=255)                                                                                                                                             tccaAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCaa                                                              <  1:716382/251‑1 (MQ=255)                                                                                                                                                aaaaaacgaactttttctgtttCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACt                                                            <  1:723304/231‑1 (MQ=255)                                                                                                                                                        gtgtAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGAt                                                                                  >  1:624756/1‑220 (MQ=255)                                                                                                                                                        gtgtAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAAc                                                     >  1:69249/1‑249 (MQ=255)                                                                                                                                                        gtgtAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACAt                                                   >  1:284637/1‑251 (MQ=255)                                                                                                                                                             aTCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAata                                              <  1:94911/251‑1 (MQ=255)                                                                                                                                                                       aTTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAg                                    <  1:587518/251‑1 (MQ=255)                                                                                                                                                                              gtccAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTaag                              >  1:752043/3‑249 (MQ=255)                                                                                                                                                                                              aGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATaaa                      <  1:654542/242‑1 (MQ=255)                                                                                                                                                                                                  tAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGacaac                                                                      >  1:734837/1‑190 (MQ=255)                                                                                                                                                                                                         caCTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGCTAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAGCATATAAAAACAAATATGTTTTTAtgcg  >  1:441258/2‑249 (MQ=255)                                                                                                                                                                                                           cttcttCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCaa                                                              >  1:779827/1‑189 (MQ=255)                                                                                                                                                                                                            ttcttcATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAAc                                                     <  1:651000/197‑1 (MQ=255)                                                                                                                                                                                                                tcATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAAc                                                     >  1:298682/1‑193 (MQ=255)                                                                                                                                                                                                                            aCTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGAt                                                                                  <  1:715891/152‑1 (MQ=255)                                                                                                                                                                                                                                           ttATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACTTTCTGCGGTAGATAGTCGTCTTTGCGGTAG‑GATTCTTGCATATTTAGGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATc                                                                                        >  1:736513/1‑131 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                   ATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAGCGAAAAGAAACTTCCCTGCTTTGACTATCATACAAAATAAAACGAGCTTTTTCTAAAAGCTGTTGTTCCGCTTCTTCCATCCAAACTACGGTGTAATCCAATTTCATTTTAAGCCCAATACTTTTTCCAGGTTAGCTAATACTTCTTCATTGGATTTGACTTTAATTTCTCCGTTATCTAGCCGTGCAAAAAATAACGCTTCTTCGGAAAGCGTCACTTTTTGTGGTAGGTAGTCATCTTTACGATAGTTATTC‑TGTGTATTTAAGCTCATAATAATTTCCTCGCTAAAAAGTTAGTCATAATCATAGATAAAACCGACAACAATAGCAACTTATTAACATAAATATGCTAATAAGATTAAACACATAAAAATAAATATGTTTTTATGTG  >  1/1455530‑1455980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑