Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,455,601	C→T	V33I (GTT→ATT)	unknown ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,455,601	0	C	T	100.0%	282.0 / NA	83	V33I (GTT→ATT)	unknown	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/42);  total (41/42)

TATTG‑TTTTTGAGGTGATATAAAGCAACGGCTATATTACAAATTCACGCCCTTTTGCCAAGAAAGCGGAAATATAAACCCTGCCATTCCGCACAATAAATTTTACGCTATGCCCGTTTTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAGCGAAAAGAAACTTCCCTGCTTTGACTATCATACAAAATAAAACGAGCTTTTTCTAAAAGCTGTTGTTCCGCTTCTTCCATCCAAACTACGGTGTAATCCAATTTCATTTTAAGCCCAATACTTTTTCCAGGTTAGCTAATACTTCTTCATTGGATTTGACTTTAATTTCTCCGTTATCTAGCCGTGCAAAAAATAACGCTTCTTCGGAAAGCGTCACTT  >  1/1455383‑1455809                                                                                                                                                                                                                            |                                                                                                                                                                                                                 tATTGTTTTTTGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATaaag                                                                                                                                                                                   <  1:780633/251‑2 (MQ=255)       tttttGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACaa                                                                                                                                                                                          <  1:861679/238‑1 (MQ=255)       tttttGAGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAg                                                                                                                                                                               >  1:565232/1‑249 (MQ=255)             aGGTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCttttt                                                                                                                                                                         >  1:36548/1‑249 (MQ=255)               gTGATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACt                                                                                                                                                                                                   >  1:497216/1‑221 (MQ=255)                 gATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACaa                                                                                                                                                                                          >  1:817324/1‑228 (MQ=255)                 gATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTaaaa                                                                                                                                                                   >  1:552574/1‑251 (MQ=255)                 gATATAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTaaaa                                                                                                                                                                   >  1:391340/1‑251 (MQ=255)                  atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGAc                                                                                                                                                                                                    <  1:535816/217‑1 (MQ=255)                  atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAAt                                                                                                                                                                                       <  1:87948/230‑1 (MQ=255)                  atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAg                                                                                                                                                                  >  1:682644/1‑251 (MQ=255)                  atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAg                                                                                                                                                                  >  1:124670/1‑251 (MQ=255)                  atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAg                                                                                                                                                                  >  1:270422/1‑251 (MQ=255)                  atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAg                                                                                                                                                                  >  1:259673/1‑251 (MQ=255)                  atatAACGCACCGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAg                                                                                                                                                                  >  1:246755/1‑251 (MQ=255)                           accGGCTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCtgctgctc                                                                                                                                                         <  1:795648/249‑7 (MQ=255)                                cTATATTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGctt                                                                                                                                                    >  1:247630/1‑251 (MQ=255)                                    atTACAAATTCCCGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCttcttc                                                                                                                                                <  1:385251/251‑1 (MQ=255)                                              cccGCCCTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAAcc                                                                                                                                      <  1:346017/249‑2 (MQ=255)                                                   ccTTTTGCTAAGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGgt                                                                                                                                 <  1:832223/251‑1 (MQ=255)                                                          ctaaGAAAGCGGAAATATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATcc                                                                                                                          <  1:466166/249‑1 (MQ=255)                                                                       aaTATAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGc                                                                                                                                                                 >  1:425909/1‑199 (MQ=255)                                                                        atatAAACCCTGTCATTCCTCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATcc                                                                                                                          >  1:588174/1‑237 (MQ=255)                                                                        atatAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTGTAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAg                                                                                                            >  1:529975/1‑251 (MQ=255)                                                                        atatAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACaa                                                                                                                                                                                          <  1:5549/173‑1 (MQ=255)                                                                        atatAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAg                                                                                                            >  1:540683/1‑251 (MQ=255)                                                                        atatAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAg                                                                                                            >  1:118251/1‑251 (MQ=255)                                                                        atatAAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAg                                                                                                            >  1:880406/1‑251 (MQ=255)                                                                         tataAACCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAgt                                                                                                           >  1:572458/1‑250 (MQ=255)                                                                              aCCCTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAAt                                                                                                      <  1:544835/251‑1 (MQ=255)                                                                               cccTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATcc                                                                                                                          >  1:143839/1‑230 (MQ=255)                                                                                ccTGTCATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATAc                                                                                                    <  1:635224/251‑1 (MQ=255)                                                                                 ctgtcATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATa                                                                                                     >  1:751239/5‑249 (MQ=255)                                                                                 ctgtcATTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATa                                                                                                     >  1:310625/5‑249 (MQ=255)                                                                                      aTTCCGCACAATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCAt                                                                                                                                                                                              <  1:846417/155‑1 (MQ=255)                                                                                              caATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACaa                                                                                                                                                                                          >  1:459089/1‑151 (MQ=255)                                                                                              caATAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAAt                                                                                                                                                                                       >  1:531208/1‑154 (MQ=255)                                                                                                 tAAATTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTa                                                                                   <  1:499693/251‑1 (MQ=255)                                                                                                    aTTTTACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAAc                                                                                                                                       <  1:168290/196‑1 (MQ=255)                                                                                                     ttttACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCtgctgctc                                                                                                                                                         <  1:421283/177‑7 (MQ=255)                                                                                                     ttttACGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTaacac                                                                               <  1:357153/251‑4 (MQ=255)                                                                                                          cGCTATGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTcc                                                                                             <  1:880829/232‑1 (MQ=255)                                                                                                              aTGCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAAt                                                                                                                                                                                       <  1:395405/138‑1 (MQ=255)                                                                                                                gCCCGTTCTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACt                                                                                                                                                                                                   <  1:245399/124‑1 (MQ=255)                                                                                                                    gttcttAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATcc                                                                                                                          <  1:107126/189‑1 (MQ=255)                                                                                                                    gttcttAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATcc                                                                                                                          <  1:236930/189‑1 (MQ=255)                                                                                                                     ttcttAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGAttta                                                               >  1:172116/4‑250 (MQ=255)                                                                                                                           aCCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTc                                                                                                                                                                        <  1:230558/140‑1 (MQ=255)                                                                                                                            ccGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGgtg                                                                                                                                >  1:854147/1‑179 (MQ=255)                                                                                                                                ataGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTtc                                                    <  1:647162/251‑1 (MQ=255)                                                                                                                                   gATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTtctc                                                  >  1:494770/1‑250 (MQ=255)                                                                                                                                    atatGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACttcttc                                                                         <  1:584562/226‑1 (MQ=255)                                                                                                                                    atatGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCg                                                >  1:623180/1‑251 (MQ=255)                                                                                                                                            tCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCt                                                                                    <  1:668694/207‑1 (MQ=255)                                                                                                                                             cGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTaac                                       <  1:96777/251‑3 (MQ=255)                                                                                                                                                    catTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAAccac                                                                                                                                    >  1:15852/1‑148 (MQ=255)                                                                                                                                                    catTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGtt                                                                                        <  1:162446/195‑1 (MQ=255)                                                                                                                                                     atTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATaaagcg                                                                                                                                                                                 >  1:336715/1‑102 (MQ=255)                                                                                                                                                     atTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCg                                     >  1:500174/1‑245 (MQ=255)                                                                                                                                                     atTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCg                                     >  1:362266/1‑245 (MQ=255)                                                                                                                                                     atTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCaaa                               >  1:313485/1‑251 (MQ=255)                                                                                                                                                      ttATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCg                                     <  1:392137/244‑1 (MQ=255)                                                                                                                                                               gTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTaacac                                                                               <  1:198665/193‑4 (MQ=255)                                                                                                                                                               gTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCaaaaaacaatac                      <  1:506439/250‑7 (MQ=255)                                                                                                                                                               gTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCaaaaaacaatac                      <  1:727580/250‑7 (MQ=255)                                                                                                                                                                   cTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGAt                                                                  <  1:275858/202‑1 (MQ=255)                                                                                                                                                                   cTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTaac                                       <  1:630338/229‑3 (MQ=255)                                                                                                                                                                   cTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCaaaaaacaatacttctt                 >  1:897615/1‑240 (MQ=255)                                                                                                                                                                     aCATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCAt                                                                                                                                                                                              >  1:146125/1‑76 (MQ=255)                                                                                                                                                                     aCATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATAc                                                                                                    >  1:268657/1‑166 (MQ=255)                                                                                                                                                                     aCATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTaac                                       <  1:545803/227‑3 (MQ=255)                                                                                                                                                                     aCATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCg                                     <  1:179950/229‑1 (MQ=255)                                                                                                                                                                       aTAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTaac                                                                                 >  1:494815/1‑182 (MQ=255)                                                                                                                                                                       aTAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTaac                                       >  1:198054/1‑223 (MQ=255)                                                                                                                                                                       aTAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCg                                     <  1:734928/227‑1 (MQ=255)                                                                                                                                                                        tAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGaa            <  1:726771/251‑1 (MQ=255)                                                                                                                                                                          ggATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCAt                                                                                                                                                                                              <  1:588071/71‑1 (MQ=255)                                                                                                                                                                          ggATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACaa                                                                                                                                                                                          <  1:214000/75‑1 (MQ=255)                                                                                                                                                                          ggATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAg          >  1:413180/1‑251 (MQ=255)                                                                                                                                                                          ggATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAg          >  1:715473/1‑251 (MQ=255)                                                                                                                                                                               gTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTaacc                                      <  1:522284/218‑4 (MQ=255)                                                                                                                                                                                  tttGGGTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGTTAGCTAACACTTCTTCATTAGATTTAACTTTAATTTCTCCGTTATCTAACCGAGCAAAAAACAATACTTCTTCGGAAAGTGTCACtt  <  1:699369/251‑1 (MQ=255)                                                                                                                                                                                      ggTCAGTTCTCTAATTTCCTGGTAAAAACGAAAAGAAATTTCCTTGCTTTGACTATCATACAAAATAAAGCGAGCTTTTTCTAAAAGCTGCTGCTCCGCTTCTTCCGTCCAAACCACGGTGTAATCCAGTTTCATTTTAAGTCCAATACTTTCTCCAGGtt                                                                                        >  1:496670/1‑161 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                 TATTG‑TTTTTGAGGTGATATAAAGCAACGGCTATATTACAAATTCACGCCCTTTTGCCAAGAAAGCGGAAATATAAACCCTGCCATTCCGCACAATAAATTTTACGCTATGCCCGTTTTTAACCGTATAGATATGAAATCGACCATCATTATAAGCGGTTGCTACATAGGATAGTTTTTGGGTCAGTTCTCTAATTTCCTGGTAAAAGCGAAAAGAAACTTCCCTGCTTTGACTATCATACAAAATAAAACGAGCTTTTTCTAAAAGCTGTTGTTCCGCTTCTTCCATCCAAACTACGGTGTAATCCAATTTCATTTTAAGCCCAATACTTTTTCCAGGTTAGCTAATACTTCTTCATTGGATTTGACTTTAATTTCTCCGTTATCTAGCCGTGCAAAAAATAACGCTTCTTCGGAAAGCGTCACTT  >  1/1455383‑1455809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑