Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,454,933	T→C	N118N (AAT→AAC)	unknown →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,454,933	0	T	C	100.0%	203.2 / NA	59	N118N (AAT→AAC)	unknown	lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/24);  total (35/24)

TTAAATAATCAGGCGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGTGCCAATATCAGTACATATCAGAAAGACCTATTAAAAAATAATGTCAATATAACTTTCAGGAAAGGAGTTAAAAATGAAAAAGCCCTTTAAAATTGGTGTAATCTTTCTTGCTTTATGCAGTATGAGCCAAATAAGCCATGCGGTGGATGAATTAACCGGTGATACCAAAACCGCCTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCG  >  1/1454691‑1455168                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            ttAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTAGATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAgcac                                                                                                                                                                                                                                     <  1:444552/251‑4 (MQ=255)            ggtgTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAgcac                                                                                                                                                                                                                                     <  1:23082/237‑4 (MQ=255)              tgTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGcacacacccaaaagac                                                                                                                                                                                                                        <  1:567149/250‑13 (MQ=255)              tgTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGcacacacccaaaagac                                                                                                                                                                                                                        <  1:380645/250‑13 (MQ=255)                  ccGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGcacacacccaaaagacttgt                                                                                                                                                                                                                    >  1:112377/1‑235 (MQ=255)                    gACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGcacacacccaaaag                                                                                                                                                                                                                          >  1:808238/1‑233 (MQ=255)                          cacCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAgcac                                                                                                                                                                                                                                     <  1:26020/226‑4 (MQ=255)                          cacCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAaat                                                                                                                                                                                                            >  1:119104/1‑251 (MQ=255)                            ccAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGAATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGCATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAgcac                                                                                                                                                                                                                                     >  1:749358/1‑221 (MQ=255)                                        gTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAatatta                                                                                                                                                                                               >  1:58620/1‑248 (MQ=255)                                           ccAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACtt                                                                                                                                                                                            <  1:489913/250‑1 (MQ=255)                                                           aTCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAaat                                                                                                                                                                                                            >  1:877183/1‑218 (MQ=255)                                                           aTCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTaaa                                                                                                                                                                           >  1:500486/1‑251 (MQ=255)                                                                        aCCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAgcac                                                                                                                                                                                                                                     >  1:738332/1‑177 (MQ=255)                                                                        aCCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGcacacacccaaaagac                                                                                                                                                                                                                        >  1:644530/1‑181 (MQ=255)                                                                        aCCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGcacacacccaaaagac                                                                                                                                                                                                                        >  1:620407/1‑181 (MQ=255)                                                                        aCCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGcacacacccaaaag                                                                                                                                                                                                                          >  1:567339/1‑181 (MQ=255)                                                                        aCCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGcc                                                                                                                                                               >  1:513522/1‑250 (MQ=255)                                                                        aCCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGcc                                                                                                                                                               >  1:636335/1‑250 (MQ=255)                                                                               gTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTaaa                                                                                                                                                        >  1:477520/1‑251 (MQ=255)                                                                                   atCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGcacacac                                                                                                                                                                                                                                 <  1:220481/173‑4 (MQ=255)                                                                                          tgtgAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAAtcgctgtgata                                                                                                                                            >  1:145910/1‑241 (MQ=255)                                                                                          tgtgAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAAtcgctgtgata                                                                                                                                            >  1:156918/1‑241 (MQ=255)                                                                                              aaCCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGcacacacccaaaagac                                                                                                                                                                                                                        >  1:532174/1‑159 (MQ=255)                                                                                               aCCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAAtcgctgtgatatgt                                                                                                                                         >  1:6629/1‑236 (MQ=255)                                                                                                 cTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAAtcgctgtgatatgtcttg                                                                                                                                     >  1:354759/1‑234 (MQ=255)                                                                                                   aGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAgcac                                                                                                                                                                                                                                     <  1:518709/153‑4 (MQ=255)                                                                                                     gTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAAtcgctgtgatatgtcttgcct                                                                                                                                  <  1:674266/250‑21 (MQ=255)                                                                                                         aaGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATg                                                                                                                              >  1:416563/1‑250 (MQ=255)                                                                                                         aaGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGc                                                                                                                             <  1:543900/251‑1 (MQ=255)                                                                                                           gCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGcacacacccaaaagac                                                                                                                                                                                                                        <  1:783160/158‑13 (MQ=255)                                                                                                              aTTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTAt                                                                                                                        <  1:792673/251‑1 (MQ=255)                                                                                                                    tgaatgaaACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGcc                                                                                                                   <  1:587181/250‑1 (MQ=255)                                                                                                                          aaaCAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCaggtaa                                                                                                             <  1:575032/250‑6 (MQ=255)                                                                                                                               gAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCaggtaagtcatg                                                                                                       >  1:104668/1‑240 (MQ=255)                                                                                                                                aaaaaGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGAGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCaggtaagtca                                                                                                         <  1:901850/248‑10 (MQ=255)                                                                                                                                              tttCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTa                                                                                         >  1:132505/1‑251 (MQ=255)                                                                                                                                                   aCTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCgg                                                                                    >  1:850524/1‑251 (MQ=255)                                                                                                                                                        gcgcATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATa                                                                               >  1:179385/1‑251 (MQ=255)                                                                                                                                                                tGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGcc                                                                                                                   >  1:138341/1‑207 (MQ=255)                                                                                                                                                                    gATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGctt                                                                   >  1:416471/1‑249 (MQ=255)                                                                                                                                                                        gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGcc                                                                                                                   <  1:103457/199‑1 (MQ=255)                                                                                                                                                                        gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCaggta                                                                                                              >  1:596469/1‑200 (MQ=255)                                                                                                                                                                        gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGc                                                                                                      >  1:671611/1‑212 (MQ=255)                                                                                                                                                                        gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGa                                                               >  1:314977/1‑251 (MQ=255)                                                                                                                                                                        gTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGa                                                               >  1:210422/1‑251 (MQ=255)                                                                                                                                                                               aaTCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAatat                                                                                                                                                                                                 >  1:95777/1‑114 (MQ=255)                                                                                                                                                                               aaTCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATAcga                                                                            >  1:26376/1‑229 (MQ=255)                                                                                                                                                                                aTCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTAc                                                       <  1:30571/251‑1 (MQ=255)                                                                                                                                                                                            ccGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGGTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCACTTGTGAAGCTCTACTCTGCTTatcat                                           <  1:442918/251‑1 (MQ=255)                                                                                                                                                                                              ggCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAga                                                                                                                                                                                       >  1:527276/1‑108 (MQ=255)                                                                                                                                                                                                      ccGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTatca                                            <  1:894063/240‑1 (MQ=255)                                                                                                                                                                                                           gCCTATAAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACgcgg                            >  1:825176/1‑251 (MQ=255)                                                                                                                                                                                                              tataAATACTGGTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTAc                                                       <  1:88811/221‑1 (MQ=255)                                                                                                                                                                                                                        ggTTACAGGCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGc                                                                                                                             <  1:239120/141‑1 (MQ=255)                                                                                                                                                                                                                                gCAAATGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCAt        <  1:168404/250‑1 (MQ=255)                                                                                                                                                                                                                                     tGGGGTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATGAATTAACCGGTGATACGAAAACCGCTTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATccgccg  >  1:338557/1‑251 (MQ=255)                                                                                                                                                                                                                                       gggTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATg                                                                                              <  1:810093/157‑1 (MQ=255)                                                                                                                                                                                                                                       gggTAAGCGCCAACATCAGCACACACCCAAAAGACTTGTTAAAAAATAATGTTAATATTACTTTCAGAAGAGGAGTTAAAAATGAAAAAGCCCTTTAAAATCGCTGTGATATGTCTTGCCTTATGCAGTATGAGCCAGGTAAGTCATGCTGTAGATg                                                                                              <  1:373705/157‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            TTAAATAATCAGGCGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCG‑TTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGTGCCAATATCAGTACATATCAGAAAGACCTATTAAAAAATAATGTCAATATAACTTTCAGGAAAGGAGTTAAAAATGAAAAAGCCCTTTAAAATTGGTGTAATCTTTCTTGCTTTATGCAGTATGAGCCAAATAAGCCATGCGGTGGATGAATTAACCGGTGATACCAAAACCGCCTGTGAAGCTCTACTCTGCTTATCATCACCAAAAGACGCGGCGAAAACGCCGGAATGTCATCCGCCG  >  1/1454691‑1455168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑