Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,454,688	T→C	Y37H (TAT→CAT)	unknown →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,454,688	0	T	C	100.0%	130.7 / NA	38	Y37H (TAT→CAT)	unknown	lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/15);  total (23/15)

TCCATTTAAGTCAGTTCTATATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAATTTTTACTTATGAATACGTTAATCTCTGCTTGTTTTTTAAGCGCGT‑GCAACTCGTCACCGCCGGAACCGCCACAAATTAATGAAGACGCGCCGGCAATCTATTTAAATAATCAGGCGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGTG  >  1/1454526‑1454928                                                                                                                                                                    |                                                                                                                                                                                                                                                 ctgtcagtccaataaattaaatcaacaAAAACTGTGAAAAACCAGGAGAAAAATAAAAAAAAAATTTTACTTATGAATATGTTAATCCCTGTTTCCTTTTAAGGCG‑GTGGCAGCTCGACACCACCAAAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATc                                                                                                                                                           <  1:408089/226‑1 (MQ=255)          caatcaatttaatCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATAtg                                                                                                                                                     >  1:393289/12‑247 (MQ=255)                               aCTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTAtgt                                                                                                                            >  1:58895/1‑251 (MQ=255)                                cTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTAtgtg                                                                                                                           >  1:398930/1‑251 (MQ=255)                                           cAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGa                                                                                                                >  1:22401/1‑251 (MQ=255)                                                ggaTAATATAAAAAAAATTTTACTTATAAATATGTTAATCCCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCCCCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATg                                                                                                                                                                             <  1:461020/185‑1 (MQ=255)                                                                   ttACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTg                                                                                         >  1:94806/1‑250 (MQ=255)                                                                      cTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCaa                                                                                                                                                         >  1:224595/1‑183 (MQ=255)                                                                      cTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGca                                                                                     >  1:892947/1‑251 (MQ=255)                                                                          tGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACtt                                                                                           >  1:499998/1‑241 (MQ=255)                                                                              tatgttaatacctgtttGTTTTTTAAGCGCGT‑GCAGCTCGTCACAACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAg                                                                             <  1:451406/236‑1 (MQ=255)                                                                                 gttaatccctttttGTTTTTTAAGCGCGT‑GCAGCTCGCCCCCACCAAAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATAtg                                                                                                                                                     <  1:705712/164‑1 (MQ=255)                                                                                 gttaatacctgtttgctttttAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGt                                                                                                                                                                >  1:603154/17‑165 (MQ=255)                                                                                 gttaatacctgtttgctttttAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGt                                                                                                                                                                >  1:670508/17‑165 (MQ=255)                                                                                 gttaatacctgtttgctttttAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATAtg                                                                                                                                                     >  1:588047/17‑176 (MQ=255)                                                                                 gttaatacctgtttgctttttAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATa                                                                          >  1:492869/17‑251 (MQ=255)                                                                                 gttaatacctgtttgctttttAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATa                                                                          >  1:265026/17‑251 (MQ=255)                                                                                 aggtgtataagagacattttaAAGCGCGT‑GCAGCTCGTCCCCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGAt                                                                           <  1:417446/234‑1 (MQ=255)                                                                                   taatacctgtttgctttttAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTAtg                                                                                                                             >  1:86519/15‑198 (MQ=255)                                                                                               gctttttAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGt                                                                                                                                                                >  1:834182/3‑151 (MQ=255)                                                                                                  ttttAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGcatc                                                                                   >  1:878525/1‑225 (MQ=255)                                                                                                  ttttAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGt                                                         >  1:80966/1‑251 (MQ=255)                                                                                                  tttaaaGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAata                                                                                                                                                       <  1:96786/153‑1 (MQ=255)                                                                                                                       tcaccaccaaaaCCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAg                                      <  1:562168/240‑1 (MQ=255)                                                                                                                          ccaccagAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATa                                                                          >  1:465040/7‑211 (MQ=255)                                                                                                                              caaaaCCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGGGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATaaa                              <  1:456126/248‑1 (MQ=255)                                                                                                                                 aaCCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATaaa                              <  1:46102/248‑1 (MQ=255)                                                                                                                                           aattgataaaGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAgg                 <  1:208992/243‑1 (MQ=255)                                                                                                                                           aattgataaaGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAgg                 <  1:33352/243‑1 (MQ=255)                                                                                                                                            attgataaaGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGc                <  1:811129/244‑1 (MQ=255)                                                                                                                                             ttgataaaGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCa               >  1:823747/7‑251 (MQ=255)                                                                                                                                                         ccccagtaaTCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGt                                                                                                                                                                <  1:800238/87‑1 (MQ=255)                                                                                                                                                         ccccagtaaTCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGaaa                                                                 <  1:25856/182‑1 (MQ=255)                                                                                                                                                         ccccagtaaTCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAg    >  1:772876/8‑250 (MQ=255)                                                                                                                                                         ccccagtaaTCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAg    >  1:420688/8‑250 (MQ=255)                                                                                                                                                          cccagtaaTCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAgcg  <  1:222502/245‑3 (MQ=255)                                                                                                                                                           ccagtaaTCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGa                                                                   >  1:85237/6‑185 (MQ=255)                                                                                                                                                           ccagtaaTCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCtat                                 >  1:585177/6‑219 (MQ=255)                                                                                                                                                                    |                                                                                                                                                                                                                                                 TCCATTTAAGTCAGTTCTATATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAATTTTTACTTATGAATACGTTAATCTCTGCTTGTTTTTTAAGCGCGT‑GCAACTCGTCACCGCCGGAACCGCCACAAATTAATGAAGACGCGCCGGCAATCTATTTAAATAATCAGGCGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGAAAAAGTTCGTTTTTTCTACTTTGCGCATCATGCAGATAGTATTGAAATCTACGGTCAGCCGGCGCGTACCGAAGCCTATAAATACTGGTTACAGGCAAATGGGGTAAGTG  >  1/1454526‑1454928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑