Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,454,457	C→T	L554L (CTA→TTA)	unknown →	Coupling protein VirD4, ATPase required for T‑DNA transfer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,454,457	0	C	T	100.0%	128.7 / NA	39	L554L (CTA→TTA)	unknown	Coupling protein VirD4, ATPase required for T‑DNA transfer
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/20);  total (19/20)

AGGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCACAGTCGAGGTAAAACATCCAGTCACAGTATCACCGACCATGGGCGACCGTTGCTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGTAAGCCAATACTGTGTAATAAAGCGGTTTACTATAACGACCCTTATTTTATTGACAAATTGAAAGCGGTTTCCCCGCAATTACGCGCACTAGGCAAGAAATTACCGACTAAAGCGCAACTGGAAAAAGCCATGTTCAGTGGTGAGCTCTCCGCCCAATCCATTTAAGTCAGTTCTATATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAATTTTTACTTATGAATACGTTAATCTCTGCTTGTTTTTTAAGCGCGTGCAACTCGTCACCGCCGGAACCGCCACAAATTAATGAAGAC  >  1/1454218‑1454675                                                                                                                                                                                                                                                |                                                                                                                                                                                                                           aagTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAggtaaaaaa                                                                                                                                                                                                                 <  1:379844/249‑8 (MQ=255)        aGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGa                                                                                                                                                                                                          >  1:146757/1‑251 (MQ=255)         ggTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGa                                                                                                                                                                                                          >  1:648891/1‑250 (MQ=255)         ggTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGa                                                                                                                                                                                                          >  1:755686/1‑250 (MQ=255)                     gTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAgaacaatt                                                                                                                                                                                             >  1:549570/1‑244 (MQ=255)                     gTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCG‑AATTGCGAGCATTAGGTAAAAAATTAcc                                                                                                                                                                                                            <  1:731237/235‑1 (MQ=255)                                   ccataGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGt                                                                                                                                                                               <  1:94956/247‑1 (MQ=255)                                   ccataGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGt                                                                                                                                                                               >  1:653968/5‑251 (MQ=255)                                          cGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAgtgg                                                                                                                                                                        <  1:769097/251‑1 (MQ=255)                                          cGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAgtgg                                                                                                                                                                        <  1:330234/251‑1 (MQ=255)                                                                gTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAAtt                                                                                                                                                  >  1:504881/1‑251 (MQ=255)                                                                   ttaCCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAggt                                                                                                                                                                                                                       >  1:285462/3‑178 (MQ=255)                                                                                 cGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTaatccacaaaaactg                                                                                                                                  <  1:47410/250‑14 (MQ=255)                                                                                         acTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAgaac                                                                                                                                                                                                 >  1:35478/2‑176 (MQ=255)                                                                                         acTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTaatccacaaaaactgtgaataac                                                                                                                          >  1:134825/2‑229 (MQ=255)                                                                                          cTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTaatccacaaaaactgtgaataacca                                                                                                                        <  1:244723/251‑24 (MQ=255)                                                                                                                         aaTTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGctgtcagtcc                                                                                                                                                          >  1:801957/1‑178 (MQ=255)                                                                                                                                        tgATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtacc                                                               <  1:403990/250‑4 (MQ=255)                                                                                                                                        tgATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtacc                                                               <  1:434082/250‑4 (MQ=255)                                                                                                                                               aaataacagcaaaccgattatatgtAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATg                                                                             <  1:136376/207‑1 (MQ=255)                                                                                                                                                        caaaccgattatatgtAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAg                                                <  1:41541/236‑1 (MQ=255)                                                                                                                                                            ccgattatatgtAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtac                                                                >  1:636159/10‑227 (MQ=255)                                                                                                                                                                    atgtAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGTGCAGCTCGt                                  >  1:412919/2‑251 (MQ=255)                                                                                                                                                                       taataaggcagtgtattacaaCGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGTGCAGCTCGTCa                                >  1:165664/20‑250 (MQ=255)                                                                                                                                                                       taataaggcagtgtattacaaCGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGTGCAGCTCGTCa                                >  1:565257/20‑250 (MQ=255)                                                                                                                                                                        aataaggcagtgtattacaaCGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGTGCAGCTCGTCAcc                              <  1:646724/233‑1 (MQ=255)                                                                                                                                                                                   gtattacaaCGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtacc                                                               <  1:614625/200‑4 (MQ=255)                                                                                                                                                                                   gtattacaaCGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtac                                                                >  1:669767/8‑204 (MQ=255)                                                                                                                                                                                   gtattacaaCGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtac                                                                <  1:745828/199‑3 (MQ=255)                                                                                                                                                                                   gtattacaaCGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAg                                                >  1:466134/8‑222 (MQ=255)                                                                                                                                                                                   gtattacaaCGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGTGCAGCTCGTCAc                               <  1:504059/232‑1 (MQ=255)                                                                                                                                                                                   gtattacaaCGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGTGCAGCTCGTCACCACCAGAAc                      >  1:643527/8‑248 (MQ=255)                                                                                                                                                                                   gtattacaaCGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGTGCAGCTCGTCACCACCAGAACCg                    >  1:659897/8‑250 (MQ=255)                                                                                                                                                                                    tattacaaCGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGTGCAGCTCGTCACCACCAGAACCGc                   <  1:817973/244‑1 (MQ=255)                                                                                                                                                                                     attacaaCGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGtt                                                                     <  1:467659/194‑1 (MQ=255)                                                                                                                                                                                              atccTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtacc                                                               <  1:777520/194‑4 (MQ=255)                                                                                                                                                                                               tccTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGTGCAGCTCGTCACCACCAGAACCGCCGCAAAttgata       >  1:274096/2‑247 (MQ=255)                                                                                                                                                                                                    tttttataGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATCCCTGTTTGTTTTTTAAGCGCGTGCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGAc  <  1:497178/250‑1 (MQ=255)                                                                                                                                                                                                      tttataGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTA‑‑‑‑‑‑‑‑‑‑‑‑ATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAg                                                <  1:233148/203‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                           AGGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCACAGTCGAGGTAAAACATCCAGTCACAGTATCACCGACCATGGGCGACCGTTGCTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGTAAGCCAATACTGTGTAATAAAGCGGTTTACTATAACGACCCTTATTTTATTGACAAATTGAAAGCGGTTTCCCCGCAATTACGCGCACTAGGCAAGAAATTACCGACTAAAGCGCAACTGGAAAAAGCCATGTTCAGTGGTGAGCTCTCCGCCCAATCCATTTAAGTCAGTTCTATATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAATTTTTACTTATGAATACGTTAATCTCTGCTTGTTTTTTAAGCGCGTGCAACTCGTCACCGCCGGAACCGCCACAAATTAATGAAGAC  >  1/1454218‑1454675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑