Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	9,584	T→C	I830V (ATT→GTT)	unknown ←	Membrane alanine aminopeptidase N (EC 3.4.11.2)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	9,584	0	T	C	100.0%	213.1 / NA	64	I830V (ATT→GTT)	unknown	Membrane alanine aminopeptidase N (EC 3.4.11.2)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/36);  total (28/36)

GCATCAAGGGCGAAAATCCCTTTACGAATCAAAGAATACATACTCGTTTATCTCTCCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAATTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAACTGCATCACATTGTTCAACACATTTTCTTCCGGGCGGCTGGCTTGCAAAGCAAA  >  1/9349‑9827                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    gCATCAAGGGCGAAAATCCCTTTACGAATCAAAGAATACATACTCGTTTATCTCTCCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTa                                                                                                                                                                                                                                       <  1:850736/250‑1 (MQ=255)         ggCGAAAATCCCTTTACGAATCAAAGAATACATACTCGTTTATCTCTCCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAg                                                                                                                                                                                                                              >  1:621580/1‑251 (MQ=255)                 tCCCTTTACGAATCAAAGAATACATACTCGTTTATCTCTCCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTg                                                                                                                                                                                                                       >  1:363289/1‑250 (MQ=255)                  cccTTTACGAATCAAAGAATACATACTCGTTTATCTCTCCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAATCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCg                                                                                                                                                                                                                     <  1:450885/251‑1 (MQ=255)                    cTTTACGAATCAAAGAATACATACTCGTTTATCTCTCCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACgg                                                                                                                                                                                                                                               <  1:341154/223‑1 (MQ=255)                           aaTCAAAGAATACATACTCGTTTATCTCTCCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCtt                                                                                                                                                                                                             <  1:634763/250‑1 (MQ=255)                                aaGAATACATACTCGTTTATCTCTCCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTc                                                                                                                                                                                                       <  1:426858/251‑1 (MQ=255)                                aaGAATACATACTCGTTTATCTCTCCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTc                                                                                                                                                                                                       <  1:634943/251‑1 (MQ=255)                                   aaTACATACTCGTTTATCTCTCCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTc                                                                                                                                                                                                       <  1:192188/248‑1 (MQ=255)                                       catacTCGTTTATCTCTCCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTaa                                                                                                                                                                                                >  1:360416/1‑251 (MQ=255)                                           cTCGTTTATCTCTCCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTc                                                                                                                                                                                                       >  1:350947/1‑240 (MQ=255)                                             cGTTTATCTCTCCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTc                                                                                                                                                                                                       >  1:355390/1‑238 (MQ=255)                                             cGTTTATCTCTCCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCaaaa                                                                                                                                                                                          >  1:263022/1‑251 (MQ=255)                                               tttATCTCTCCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTCTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAat                                                                                                                                                                                         <  1:441118/250‑1 (MQ=255)                                                   tctctcCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCg                                                                                                                                                                                    <  1:508573/251‑1 (MQ=255)                                                     tctcCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTc                                                                                                                                                                                                       >  1:59335/1‑230 (MQ=255)                                                     tctcCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCCCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGtt                                                                                                                                                                                  >  1:254103/1‑251 (MQ=255)                                                      ctcCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTc                                                                                                                                                                                                       <  1:736347/229‑1 (MQ=255)                                                             tAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACg                                                                                                                                                                          >  1:214356/1‑251 (MQ=255)                                                               gTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGAt                                                                                                                                                                        >  1:673743/1‑251 (MQ=255)                                                                         gAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAAc                                                                                                                                                               <  1:889719/250‑1 (MQ=255)                                                                                         tCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAg                                                                                                                                              <  1:659173/251‑1 (MQ=255)                                                                                                  aCCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTaaa                                                                                                                                     <  1:506819/251‑1 (MQ=255)                                                                                                        cTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTg                                                                                                                                >  1:897379/1‑250 (MQ=255)                                                                                                          ttttGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGccc                                                                                                                             <  1:38128/251‑1 (MQ=255)                                                                                                               atatTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGaa                                                                                                                                               <  1:568550/228‑1 (MQ=255)                                                                                                               atatTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGa                                                                                                                        >  1:828439/1‑251 (MQ=255)                                                                                                               atatTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGa                                                                                                                        >  1:476600/1‑251 (MQ=255)                                                                                                                  ttACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCga                                                                                                                                                                                                                                  >  1:770991/1‑142 (MQ=255)                                                                                                                  ttACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAAt                                                                                                                                    <  1:470864/236‑1 (MQ=255)                                                                                                                    aCTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGa                                                                                                                                                                         <  1:252197/197‑1 (MQ=255)                                                                                                                     cTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGaa                                                                                                                                               <  1:414760/222‑1 (MQ=255)                                                                                                                     cTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAAttt                                                                                                                                  >  1:792241/1‑235 (MQ=255)                                                                                                                               ttttCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGc                                                                                                                                                     <  1:16932/206‑1 (MQ=255)                                                                                                                                    gATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAAt                                                                                                                                    >  1:211880/1‑218 (MQ=255)                                                                                                                                       tttCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTacac                                                                                                <  1:667919/251‑1 (MQ=255)                                                                                                                                        ttCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGttt                                                                                                                                                                                                  >  1:535880/1‑152 (MQ=255)                                                                                                                                            cGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGAtt                                                                                            >  1:384789/1‑250 (MQ=255)                                                                                                                                               aCAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCTGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGa                                                                                        <  1:535957/251‑1 (MQ=255)                                                                                                                                                            tAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTattgaaattga                                                                           <  1:674353/251‑1 (MQ=255)                                                                                                                                                                tCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCt                                                                       <  1:85904/251‑1 (MQ=255)                                                                                                                                                                              gATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTa                                                         >  1:617875/1‑251 (MQ=255)                                                                                                                                                                                tACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAAc                                                       <  1:654556/251‑1 (MQ=255)                                                                                                                                                                                 aCGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAACt                                                      <  1:836732/251‑1 (MQ=255)                                                                                                                                                                                  cGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAAc                                                       >  1:210809/1‑249 (MQ=255)                                                                                                                                                                                  cGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAACTg                                                     <  1:546197/251‑1 (MQ=255)                                                                                                                                                                                   gTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTc                                                                                                                                                                                                       <  1:869695/104‑1 (MQ=255)                                                                                                                                                                                   gTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGaa                                                                                                                                               <  1:859373/160‑1 (MQ=255)                                                                                                                                                                                   gTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGc                                                                                                                  >  1:893150/1‑189 (MQ=255)                                                                                                                                                                                   gTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAACTg                                                     >  1:558822/1‑250 (MQ=255)                                                                                                                                                                                   gTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAACTg                                                     >  1:760615/1‑250 (MQ=255)                                                                                                                                                                                   gTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAACTg                                                     >  1:278047/1‑250 (MQ=255)                                                                                                                                                                                                tttCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAACTGCATCACATTGTTc                                        <  1:78362/250‑1 (MQ=255)                                                                                                                                                                                                tttCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAACTGCATCACATTGTTc                                        <  1:805990/250‑1 (MQ=255)                                                                                                                                                                                                    atcaACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCTGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAACTGCATCACATTGTTCAacac                                   <  1:312220/251‑1 (MQ=255)                                                                                                                                                                                                    atcaACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAACTGCATCACATTGTTCAacac                                   <  1:518287/251‑1 (MQ=255)                                                                                                                                                                                                    atcaACGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAACTGCATCACATTGTTCAacac                                   <  1:887760/251‑1 (MQ=255)                                                                                                                                                                                                        aCGTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATgg                                                                                                                                                      <  1:84929/132‑1 (MQ=255)                                                                                                                                                                                                          gTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTacac                                                                                                <  1:334231/184‑1 (MQ=255)                                                                                                                                                                                                          gTTTGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAACTGCATCACATTGTTCAACACAtttt                              >  1:276815/1‑250 (MQ=255)                                                                                                                                                                                                             tGGCGTTGCGCATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAACTGCATCACATTGTTCAACACATTttcttc                          <  1:708715/251‑1 (MQ=255)                                                                                                                                                                                                                    gcgcATCATAACGGACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAACTGCATCACAt                                             >  1:770895/1‑225 (MQ=255)                                                                                                                                                                                                                                 gACAAAACGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAACTGCATCACATTGTTCAACACATTTTCTTCCGGGCGGCTGGCttacacca      >  1:173861/1‑245 (MQ=255)                                                                                                                                                                                                                                     aaaCGAACTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAACTGCATCACATTGTTCAACACATTTTCTTCCGGGCGGCTGGCTTgcaaagcaaa  >  1:793036/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    GCATCAAGGGCGAAAATCCCTTTACGAATCAAAGAATACATACTCGTTTATCTCTCCGGATAGTTAACTGTGGAATGAAAAAGTGCGGTCAAAATTAACCGCACTTTTTGATATTACTGTAATGCTTTTTCGATTTTCTCGTACAAATCGCGGGATAAATCTTCCGTTTCGCTGATACGTTCTAAGGCGCGTTTCATCAACGTTTGGCGTTGCGCATCATAACGGACAAAACGAATTAACGGTTCAATTAAGCGAGAAGCTACTTGCGGGTTGCTTTTATTCAGTTTAATCAAAATATCCGTTAAGAAACGATAGCCGGAACCGTCAATGGCATGGAAGGCTTTTAAATTTTGCCCTGCGAATGTGCCGACTAACGAACGTACACGATTCGGATTATTGAAATTGAAGCTCGGGTGCTCCATTAACTGCATCACATTGTTCAACACATTTTCTTCCGGGCGGCTGGCTTGCAAAGCAAA  >  1/9349‑9827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑