Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	166,994	C→T	P39L (CCA→CTA)	unknown →	Outer membrane beta‑barrel assembly protein BamC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	166,994	0	C	T	100.0%	272.1 / NA	80	P39L (CCA→CTA)	unknown	Outer membrane beta‑barrel assembly protein BamC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/44);  total (36/44)

CGCCGGCTTGCTTTAAACGCAGTATTCATCAGGACCTATTCATATAGGCCCCGAACTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACCATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCACTGATCGCTTATACGCCGGACAAACAAAGAGTTTACAATCTAACCCAAGTGGAACGCCTGCTCAAAGAACAAGACAT  >  1/166750‑167240                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       cgccgGCTTGCTTTAAACGCAGTATTCATCAGGACCTATTCATATAGGCCCCGAACTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATAttt                                                                                                                                                                                                                                                  <  1:215461/251‑1 (MQ=255)       cttgcttTAAACGCAGTATTCATCAGGACCTATTCATATAGGCCCCGAACTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTc                                                                                                                                                                                                                                             <  1:282133/250‑1 (MQ=255)          gcttTAAACGCAGTATTCATCAGGACCTATTCATATAGGCCCCGAACTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGtt                                                                                                                                                                                                                                         >  1:88047/1‑251 (MQ=255)                            atcaGGACCTATTCATATAGGCCCCGAACTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGaa                                                                                                                                                                                                                       >  1:279827/1‑251 (MQ=255)                                    cTATTCATATAGGCCCCGAACTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCgg                                                                                                                                                                                                               >  1:294642/1‑251 (MQ=255)                                     tATTCATATAGGCCCCGAACTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGcc                                                                                                                                                                                                                                     <  1:413364/228‑1 (MQ=255)                                      aTTCATATAGGCCCCGAACTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTggc                                                                                                                                                                                                                               <  1:583716/233‑1 (MQ=255)                                      aTTCATATAGGCCCCGAACTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGca                                                                                                                                                                                                             >  1:483147/1‑251 (MQ=255)                                      aTTCATATAGGCCCCGAACTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGca                                                                                                                                                                                                             >  1:167432/1‑251 (MQ=255)                                         cATATAGGCCCCGAACTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTAc                                                                                                                                                                                                                                               >  1:128087/1‑214 (MQ=255)                                           tataGGCCCCGAACTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAg                                                                                                                                                                                                        <  1:92143/251‑1 (MQ=255)                                               ggCCCCGAACTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTg                                                                                                                                                                                                                   <  1:163327/236‑1 (MQ=255)                                               ggCCCCGAACTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGcgcg                                                                                                                                                                                                    >  1:537784/1‑251 (MQ=255)                                                gCCCCGAACTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCgg                                                                                                                                                                                                   >  1:293204/1‑251 (MQ=255)                                                     gAACTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATAtttt                                                                                                                                                                                                                                                 <  1:262209/200‑1 (MQ=255)                                                        cTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACTTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCaa                                                                                                                                                                                           <  1:300734/251‑1 (MQ=255)                                                        cTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCaa                                                                                                                                                                                           <  1:453826/251‑1 (MQ=255)                                                        cTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCaa                                                                                                                                                                                           <  1:427746/251‑1 (MQ=255)                                                          gTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTAc                                                                                                                                                                                                                                               >  1:164756/1‑197 (MQ=255)                                                          gTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAAtt                                                                                                                                                                                         >  1:416337/1‑251 (MQ=255)                                                          gTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAAtt                                                                                                                                                                                         >  1:399433/1‑251 (MQ=255)                                                          gTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAAtt                                                                                                                                                                                         >  1:507257/1‑251 (MQ=255)                                                          gTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAAtt                                                                                                                                                                                         >  1:591411/1‑251 (MQ=255)                                                          gTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAAtt                                                                                                                                                                                         >  1:466036/1‑251 (MQ=255)                                                                acaTTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTaccacc                                                                                                                                                                                   <  1:310919/251‑1 (MQ=255)                                                                acaTTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTaccacc                                                                                                                                                                                   <  1:717099/251‑1 (MQ=255)                                                                 caTTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATc                                                                                                                                                                                                                     >  1:711634/1‑216 (MQ=255)                                                                    tattCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGtt                                                                                                                                                                               <  1:334267/251‑1 (MQ=255)                                                                           tCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATc                                                                                                                                                                         <  1:152976/250‑1 (MQ=255)                                                                           tCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATc                                                                                                                                                                         <  1:885682/250‑1 (MQ=255)                                                                           tCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATc                                                                                                                                                                         <  1:857360/250‑1 (MQ=255)                                                                                      cAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGAc                                                                                                                                                             <  1:660188/251‑1 (MQ=255)                                                                                        aTTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACgg                                                                                                                                                           <  1:842409/251‑1 (MQ=255)                                                                                           tttACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGgtgt                                                                                                                                                        <  1:561935/251‑1 (MQ=255)                                                                                              aCGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGa                                                                                                                                                     <  1:266869/251‑1 (MQ=255)                                                                                                 cAACATCCTAATCATGAGGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGata                                                                                                                                                   <  1:524222/250‑1 (MQ=255)                                                                                                 cAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGatat                                                                                                                                                  >  1:15221/1‑251 (MQ=255)                                                                                                 cAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGata                                                                                                                                                   <  1:76306/250‑1 (MQ=255)                                                                                                      tCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGt                                                                                                                                              >  1:887897/1‑250 (MQ=255)                                                                                                      tCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGt                                                                                                                                              >  1:184377/1‑250 (MQ=255)                                                                                                            tCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATc                                                                                                                                                                         <  1:459490/217‑1 (MQ=255)                                                                                                                         gggTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTgg                                                                                                                           >  1:174938/1‑250 (MQ=255)                                                                                                                           gTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCa                                                                                                                         <  1:55095/250‑1 (MQ=255)                                                                                                                                  aTGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAg                                                                                                                 <  1:488757/251‑1 (MQ=255)                                                                                                                                     aaaaaaTGGCTATTAACCGTAGCAATCGTCGCCGCACTCAGCGCCTGCACCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTAGCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTaa                                                                                                              <  1:317827/251‑1 (MQ=255)                                                                                                                                     aaaaaaTGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTa                                                                                                               <  1:45739/250‑1 (MQ=255)                                                                                                                                        aaaTGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTc                                                                                                           <  1:885910/251‑1 (MQ=255)                                                                                                                                        aaaTGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTc                                                                                                           <  1:875205/251‑1 (MQ=255)                                                                                                                                            ggCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATAtt                                                                                                                                                 <  1:584882/209‑1 (MQ=255)                                                                                                                                            ggCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGt                                                                                                        >  1:178176/1‑250 (MQ=255)                                                                                                                                             gCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTGCTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCaa                                                                                                                                                                                           <  1:378409/166‑1 (MQ=255)                                                                                                                                                aTTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATAtt                                                                                                                                                 <  1:799834/205‑1 (MQ=255)                                                                                                                                                   aaCCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTc                                                                                                           >  1:457966/1‑240 (MQ=255)                                                                                                                                                     ccGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGata                                                                                                                                                   >  1:337625/1‑198 (MQ=255)                                                                                                                                                           cAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACgg                                                                                         <  1:66353/250‑1 (MQ=255)                                                                                                                                                                 tCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGcc                                                                                                                                >  1:567750/1‑205 (MQ=255)                                                                                                                                                                     cGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCAc                                                                              <  1:46173/251‑1 (MQ=255)                                                                                                                                                                     cGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCAc                                                                              <  1:603910/251‑1 (MQ=255)                                                                                                                                                                     cGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCAc                                                                              <  1:273989/251‑1 (MQ=255)                                                                                                                                                                       cACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAg                                                                                                                                                                                                        <  1:13215/127‑1 (MQ=255)                                                                                                                                                                        aCTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAatcatc                                                                                                                   >  1:689799/1‑211 (MQ=255)                                                                                                                                                                        aCTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACAGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCACTGa                                                                           >  1:447193/1‑251 (MQ=255)                                                                                                                                                                         cTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTaaaa                                                                                                                                                                           >  1:155995/1‑154 (MQ=255)                                                                                                                                                                             gcgcCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATAtt                                                                                                                                                 <  1:163408/176‑1 (MQ=255)                                                                                                                                                                                 cTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTaa                                                                                                              >  1:605264/1‑207 (MQ=255)                                                                                                                                                                                         ccGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCACTGATCGCTTATACGCCGGac                                                          <  1:820556/251‑1 (MQ=255)                                                                                                                                                                                                  aaaGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTAc                                                                                                                                                                                                                                               <  1:307539/61‑1 (MQ=255)                                                                                                                                                                                                     gTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCAc                                                                              >  1:802116/1‑219 (MQ=255)                                                                                                                                                                                                         acaacaAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCACTGATCGCTTATACGCCGGACAAACAAAGAGTTTACa                                          >  1:820688/1‑251 (MQ=255)                                                                                                                                                                                                          caacaaGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCAc                                                                              >  1:579037/1‑214 (MQ=255)                                                                                                                                                                                                                 cAGGTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCACTGATCGCTTATACGCCGGACAAACAAAGAGTTTACAATCTAAc                                   <  1:865618/250‑1 (MQ=255)                                                                                                                                                                                                                   ggTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCaa                                                                                                                                                                                           <  1:149652/96‑1 (MQ=255)                                                                                                                                                                                                                   ggTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCACTGATCGCTtata                                                                  <  1:766410/217‑1 (MQ=255)                                                                                                                                                                                                                   ggTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCACTGATCGCTTATACGCCGGACAAACAAAGAGTTTACAATCTAACCCa                                >  1:828391/1‑251 (MQ=255)                                                                                                                                                                                                                   ggTGATACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGACGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACAACCAGTAAAAATTAGCAGAACCGACGGCGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCACTGATCGCTTATACGc                                                               >  1:54496/1‑220 (MQ=255)                                                                                                                                                                                                                       aTACCTATCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCACTGATCGCTTATACGCCGGACAAACAAAGAGTTTACAATCTAACCCAAGTg                            >  1:320901/1‑251 (MQ=255)                                                                                                                                                                                                                             aTCAAAAATCCAACAGCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCACTGATCGCTTATACGCCGGACAAACAAAGAGTTTACAATCTAACCCAAGTGGAACGc                      <  1:390601/251‑1 (MQ=255)                                                                                                                                                                                                                                            gCGAATTACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCACTGATCGCTTATACGCCGGACAAACAAAGAGTTTACAATCTAACCCAAGTGGAACGCCTGCTCAAAGAACaa       >  1:436367/1‑251 (MQ=255)                                                                                                                                                                                                                                                 ttACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCACTGATCGCTTATACGCCGGACAAACAAAGAGTTTACAATCTAACCCAAGTGGAACGCCTGCTCAAAGAACAAGACAt  >  1:323296/1‑251 (MQ=255)                                                                                                                                                                                                                                                 ttACTATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCACTGATCGCTTATACGCCGGACAAACAAAGAGTTTACAATCTAACCCAAGTGGAACGCCTGCTCAAAGAACAAGACAt  >  1:748267/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       CGCCGGCTTGCTTTAAACGCAGTATTCATCAGGACCTATTCATATAGGCCCCGAACTGTTCACACATTATTCTGTCGAAAAAAAACAATTTTTACGCAACATCCTAATCATGAAGCAAAAGGGTAATTTATGAAAAAATGGCTATTAACCGTAGCAATCGTCACCGCACTCAGCGCCTGCTCCACCGGTAACGAAAGTAAACAACAAGCAGGTGATACCTATCAAAAATCCAACAGCGAATTACCATATTTTACTCCGTTAGCCAGTGGCGGCGTGAATCTGCCGGCACAAAGCGCGGAATATCAATTACCACCAGTTAAAATCAGCAGAACCGACGGTGTAGATATTCGTCCGCCATCCTTGCCATTGGCAATCATCAGTAATTCCGTCACACAATTTGACGGCGAACGTGCACTGATCGCTTATACGCCGGACAAACAAAGAGTTTACAATCTAACCCAAGTGGAACGCCTGCTCAAAGAACAAGACAT  >  1/166750‑167240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑