Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,453,832	C→T	A345A (GCC→GCT)	unknown →	Coupling protein VirD4, ATPase required for T‑DNA transfer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,453,832	0	C	T	100.0%	252.5 / NA	74	A345A (GCC→GCT)	unknown	Coupling protein VirD4, ATPase required for T‑DNA transfer
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/40);  total (34/40)

CAAGAAGACGGGTCAATAGAACATATCGATTTTGCGCAACACGCGCGGTTATGCGTTCAATCCGGCATTGCTCACCAAACTACCGCCGATAAACTCAATATTTATTTAATGGCGTGCGAATCCGGTAAAACAAAAAGTAGTATCGATTCAACTTTTACCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAACATGCGACTTCTGCCAGTGATTTTGATTTACGCGCCTTGCGCCGGGAAAAAATCACGATTTATTTCCACATTTCAGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCGGCATTAAAATATCAGTTATTACTACTCATGGATGAATTTACTGCGGTCGGGATGTTGAGCATTATCAATAAATCAGTAGGATTTATTGCCGGT  >  1/1453602‑1454057                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                  cAAGAAGACGGTTCGATAGAACATATCGTTTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGCGAATCCGGTAAAACAAAAAACAGTATCGATTCAACCTTTATCAGTCCGCTAACCATTTTTCAAAATGAAATTGTTGAATTTGCGACTTCTGCCCGTGATTTTGTTTTACGCGCTTTACGCAAGGAAAAAATcc                                                                                                                                                                                                                >  1:608590/1‑249 (MQ=255) cAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCa                                                                                                                                                                                                                >  1:374650/1‑250 (MQ=255)                aTAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACAt                                                                                                                                                                                                <  1:792956/251‑1 (MQ=255)                    aaCATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTcg                                                                                                                                                                                            >  1:593985/1‑250 (MQ=255)                                    acAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATcc                                                                                                                                                                            <  1:62452/250‑1 (MQ=255)                                        catgcgcgcTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGGGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTgg                                                                                                                                                                         >  1:703888/4‑250 (MQ=255)                                         atgcgcgcTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATc                                                                                                                                                                             >  1:258321/3‑245 (MQ=255)                                                cttATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATc                                                                                                                                                                             >  1:767467/2‑238 (MQ=255)                                                  tATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGt                                                                                                                                                               >  1:617929/1‑250 (MQ=255)                                                              ccGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGttt                                                                                                                                                  <  1:221261/251‑1 (MQ=255)                                                                 gCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATc                                                                                                                                                       <  1:9428/243‑1 (MQ=255)                                                                   aTTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAg                                                                                                                                             <  1:211715/251‑1 (MQ=255)                                                                    ttGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATcc                                                                                                                                                                            <  1:594534/219‑1 (MQ=255)                                                                      gCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTTTTGAATATGCGACTTCTGCCAGGGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCAt                                                                                                                                          >  1:898984/1‑251 (MQ=255)                                                                      gCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCAt                                                                                                                                          >  1:556728/1‑251 (MQ=255)                                                                      gCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCAt                                                                                                                                          >  1:516079/1‑251 (MQ=255)                                                                        tCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGAAAAAAATCACGATTTATTTc                                                                                                                                                                                                     >  1:439153/1‑190 (MQ=255)                                                                         cACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAg                                                                                                                                             >  1:891959/1‑245 (MQ=255)                                                                         cACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGt                                                                                                                                       <  1:778923/251‑1 (MQ=255)                                                                           ccagactactGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTa                                                                                                                                     >  1:881987/5‑251 (MQ=255)                                                                              gactactGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCaa                                                                                                                                                                 <  1:61321/219‑1 (MQ=255)                                                                              gactactGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTg                                                                                                                                  >  1:581931/2‑251 (MQ=255)                                                                                cTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGcc                                                                                                                                <  1:820847/251‑1 (MQ=255)                                                                                cTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGcc                                                                                                                                <  1:275739/251‑1 (MQ=255)                                                                                  actgccgATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAg                                                                                                                                             <  1:376767/233‑1 (MQ=255)                                                                                     gccgATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCcaacaa                                                                                                                           >  1:107861/1‑251 (MQ=255)                                                                                      ccgATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAat                                                                                                                          >  1:186430/1‑251 (MQ=255)                                                                                         aTAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAatat                                                                                                                        <  1:332869/250‑1 (MQ=255)                                                                                                cAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGcc                                                                                                                                <  1:761522/235‑1 (MQ=255)                                                                                                      ttatttaATGGCGTGTGAATCCGGTAAGACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCgg                                                                                                          >  1:100805/1‑251 (MQ=255)                                                                                                      ttatttaATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATc                                                                                                                                                       <  1:145161/206‑1 (MQ=255)                                                                                                        atttaATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGcc                                                                                                                                <  1:66185/227‑1 (MQ=255)                                                                                                        atttaATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGa                                                                                                                     >  1:747595/1‑238 (MQ=255)                                                                                                        atttaATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGaa                                                                                                        >  1:413599/1‑251 (MQ=255)                                                                                                           taATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAaacc                                                                                                     <  1:267617/251‑1 (MQ=255)                                                                                                                    gtgAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAAcccaaca                                                                                            >  1:249560/3‑247 (MQ=255)                                                                                                                          ccGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTaaaa                                                                                      <  1:397684/251‑1 (MQ=255)                                                                                                                          ccGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTaaaa                                                                                      <  1:2814/251‑1 (MQ=255)                                                                                                                           cGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATcc                                                                                                                                                                            >  1:411356/1‑164 (MQ=255)                                                                                                                              tAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAatat                                                                                   <  1:517120/250‑1 (MQ=255)                                                                                                                                  aCAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGtt                                                                              <  1:453448/251‑1 (MQ=255)                                                                                                                                  aCAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGtt                                                                              >  1:459126/1‑251 (MQ=255)                                                                                                                                       aagcagtaTCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAAt                                                                                                                                                                                                                  <  1:549789/110‑1 (MQ=255)                                                                                                                                       aagcagtaTCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGc                                                                                                                                                                        <  1:832096/152‑1 (MQ=255)                                                                                                                                            gtaTCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATg                                                                                                                                               >  1:700238/1‑176 (MQ=255)                                                                                                                                             taTCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATtactac                                                                      <  1:542122/248‑1 (MQ=255)                                                                                                                                               tCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATg                                                                 <  1:238581/251‑1 (MQ=255)                                                                                                                                                cGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATgg                                                                >  1:596482/1‑251 (MQ=255)                                                                                                                                                 gATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATc                                                                                                                                                                             <  1:298752/141‑1 (MQ=255)                                                                                                                                                 gATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATc                                                                                                                                                       <  1:74762/163‑1 (MQ=255)                                                                                                                                                  aTTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTcggc                                                                                                                                                                                          >  1:856483/1‑124 (MQ=255)                                                                                                                                                      aacctttATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATtact                                                                        <  1:252071/233‑1 (MQ=255)                                                                                                                                                       acctttATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATc                                                                                                                                                       <  1:601834/154‑1 (MQ=255)                                                                                                                                                       acctttATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATg                                                                                                                                               <  1:564016/162‑1 (MQ=255)                                                                                                                                                       acctttATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAatat                                                                                                                        >  1:708687/4‑188 (MQ=255)                                                                                                                                                       acctttATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGagtt                                                         >  1:195089/4‑248 (MQ=255)                                                                                                                                                       acctttATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGagt                                                          >  1:48118/4‑248 (MQ=255)                                                                                                                                                         ctttATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAAcccaacat                                                                                           >  1:164068/2‑210 (MQ=255)                                                                                                                                                           ttatcAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAat                                                                                     <  1:386040/215‑1 (MQ=255)                                                                                                                                                                   ccGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATg                                                             <  1:465579/235‑1 (MQ=255)                                                                                                                                                                        aaCCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGt                                        <  1:570178/251‑1 (MQ=255)                                                                                                                                                                          ccATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTg                                      <  1:518562/251‑1 (MQ=255)                                                                                                                                                                          ccATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTg                                      >  1:285079/1‑251 (MQ=255)                                                                                                                                                                             tttttCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGc                                   <  1:509584/251‑1 (MQ=255)                                                                                                                                                                               tttCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCAt                                 <  1:632859/251‑1 (MQ=255)                                                                                                                                                                                  cAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTAt                              <  1:608420/251‑1 (MQ=255)                                                                                                                                                                                       tGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATa                         >  1:786649/1‑251 (MQ=255)                                                                                                                                                                                              gttgAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCAt                                 <  1:598106/236‑1 (MQ=255)                                                                                                                                                                                                  aataTGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAgg               <  1:135605/247‑1 (MQ=255)                                                                                                                                                                                                   ataTGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATtact                                                                        <  1:63564/190‑1 (MQ=255)                                                                                                                                                                                                   ataTGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATg                                                                 >  1:240801/3‑199 (MQ=255)                                                                                                                                                                                                          aCTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTAtcgc      <  1:48175/251‑4 (MQ=255)                                                                                                                                                                                                             tCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCgg   >  1:844166/1‑251 (MQ=255)                                                                                                                                                                                                              cTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGt  >  1:752925/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                  CAAGAAGACGGGTCAATAGAACATATCGATTTTGCGCAACACGCGCGGTTATGCGTTCAATCCGGCATTGCTCACCAAACTACCGCCGATAAACTCAATATTTATTTAATGGCGTGCGAATCCGGTAAAACAAAAAGTAGTATCGATTCAACTTTTACCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAACATGCGACTTCTGCCAGTGATTTTGATTTACGCGCCTTGCGCCGGGAAAAAATCACGATTTATTTCCACATTTCAGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCGGCATTAAAATATCAGTTATTACTACTCATGGATGAATTTACTGCGGTCGGGATGTTGAGCATTATCAATAAATCAGTAGGATTTATTGCCGGT  >  1/1453602‑1454057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑