Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	162,351	C→T	intergenic (+186/‑40)	unknown → / → unknown	GTP‑binding protein TypA/BipA/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	162,351	0	C	T	100.0%	21.1 / NA	8	intergenic (+186/‑40)	unknown/unknown	GTP‑binding protein TypA/BipA/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/5);  total (3/5)

TCTATAATTTACATTTCTATTTCCTATTAAAAATATATTCAAAAAAAAGAATATTGAGGATATAATGAAGAAAGTTTAGTTGTGTTCTTTTTCAAAAAAGTACTGCATTGTAATATACTTAACATCTTATTTTTATATAACTATAAATCCTAACTCTATGAAAATTTTACTGAAACCTTTCCGCTATTCTGTTATAGCAACAACCATCGCATTGGTATTTAATCAACCGGCATTTGCGTCAGAGTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTT  >  1/162234‑162588                                                                                                                      |                                                                                                                                                                                                                                              cggctaATTTACATTTCTATTTCCTATTAAAAATATATTCAAAAAAAAGAATATTGAGGATATAATGAAGAAAGTTTAGTTGTGTTCTTTTTCAAAAAAGTACTGCATTGTAATATATTTAACATCTTATTTTTATATAACTATAAATCCTAACTCTATGAAAATTTTACTGAAACCTTTCCGCTATTCTGTTATAGCAACAACCATCGCATTGGTATTTAATCAACCGGCATTTGCGTCAGAGTTTAATg                                                                                                          <  1:820784/247‑1 (MQ=255)  cTATAATTTACATTTCTATTTCCTATTAAAAATATATTCAAAAAAAAGAATATTGAGGATATAATGAAGAAAGTTTAGTTGTGTTCTTTTTCAAAAAAGTACTGCATTGTAATATATTTAACATCTTATTTTTATATAACTATAAATCCTAACTCTATGAAAATTTTACTGAAACCTTTCCGCTATTCTGTTATAGCAACAACCATCGCATTGGTATTTAATCAACCGGCATTTGCGTCAGAGTTTAATGc                                                                                                         <  1:85890/251‑1 (MQ=255)                                                   atatTGAGGATATAATGAAGAAAGTTTAGTTGTGTTCTTTTTCAAAAAAGTACTGCATTGTAATATATTTAACATCTTATTTTTATATAACTATAAATCCTAACTCTATGAAAATTTTACTGAAACCTTTCCGCTATTCTGTTATAGCAACAACCATCGCATTGGTATTTAATCAACCGGCATTTGCGTCAGAGTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAAcc                                                        <  1:837951/251‑1 (MQ=255)                                                           gATATAATGAAGAAAGTTTAGTTGTGTTCTTTTTCAAAAAAGTACTGCATTGTAATATATTTAAc                                                                                                                                                                                                                                          >  1:13927/1‑65 (MQ=255)                                                                   gaagaaAGTTTAGTTGTGTTCTTTTTCAAAAAAGTACTGCATTGTAATATATTTAACATCTTATTTTTATATAACTATAAAt                                                                                                                                                                                                                 >  1:624866/1‑82 (MQ=255)                                                                       aaaGTTTAGTTGTGTTCTTTTTCAAAAAAGTACTGCATTGTAATATATTTAACATCTTATTTTTATATAACTATAAATCCTAACTCTATGAAAATTTTACTGAAACCTTTCCGCTATTCTGTTATAGCAACAAcc                                                                                                                                                        >  1:741255/1‑135 (MQ=255)                                                                                                          cATTGTAATATATTTAACATCTTATTTTTATATAACTATAAATCCTAACTCTATGAAAATTTTACTGAAACCTTTCCGCTATTCTGTTATAGCAACAACCATCGCATTGGTATTTAATCAACCGGCATTTGCGTCAGAGTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGtt  <  1:402166/250‑1 (MQ=255)                                                                                                            ttGTAATATATTTAACATCTTATTTTTATATAACTATAAATCCTAACTCTATGAAAATTTTACTGAAACCTTTCCGCTATTCTGTTATAGCAACAACCATCGCATTGGTATTTAATCAACCGGCATTTGCGTCAGAGTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACat                           <  1:637463/223‑1 (MQ=255)                                                                                                                      |                                                                                                                                                                                                                                              TCTATAATTTACATTTCTATTTCCTATTAAAAATATATTCAAAAAAAAGAATATTGAGGATATAATGAAGAAAGTTTAGTTGTGTTCTTTTTCAAAAAAGTACTGCATTGTAATATACTTAACATCTTATTTTTATATAACTATAAATCCTAACTCTATGAAAATTTTACTGAAACCTTTCCGCTATTCTGTTATAGCAACAACCATCGCATTGGTATTTAATCAACCGGCATTTGCGTCAGAGTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTT  >  1/162234‑162588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑