| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,453,572 | C→T | L259L (CTA→TTA) | unknown → | Coupling protein VirD4, ATPase required for T‑DNA transfer |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,453,572 | 0 | C | T | 100.0% | 236.9 / NA | 68 | L259L (CTA→TTA) | unknown | Coupling protein VirD4, ATPase required for T‑DNA transfer |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (39/29); total (39/29) | |||||||||||
GCTTTGCCCCATTTGACCGTAACACGCACCGATTTAATCCATTGGATTGTATTGATATGCGCGACCGAGCACGCGCCGAACTCGATTTAAAAAATCAGGCAATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAAAATCTTTTTGTGGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACTGATATGACACTGACTTACGCCAATCTACTACGTTTACACGCAGGTTTTGACGAAACGCAAGAAGACGGGTCAATAGAACATATCGATTTTGCGCAACACGCGCGGTTATGCGTTCAATCCGGCATTGCTCACCAAACTACCGCCGATAAACTCAATATTTATTTAATGGCGTGCGAATCCGGTAAAACAAAAAGTAGTATCGATTCAACTTTTACCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAACATGCGACTTCTGCCAGTGATT > 1/1453327‑1453818 | gCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACg > 1:176680/1‑250 (MQ=255) gTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACg > 1:824609/1‑232 (MQ=255) gTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGat > 1:131293/1‑250 (MQ=255) atacGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACgc < 1:336539/228‑2 (MQ=255) gtttAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCaagaag > 1:867919/2‑251 (MQ=255) aTTGTATTGATATGCGCGACTGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGaa > 1:877063/1‑251 (MQ=255) aTTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGaa > 1:679362/1‑251 (MQ=255) ttGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAAc < 1:788236/251‑1 (MQ=255) gTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTacatgc < 1:667534/211‑4 (MQ=255) gTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACat > 1:739291/1‑251 (MQ=255) gTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACat > 1:576297/1‑251 (MQ=255) aTTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACggttcgat < 1:800839/242‑7 (MQ=255) gATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGAc < 1:873464/231‑1 (MQ=255) atGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAg < 1:369278/238‑1 (MQ=255) atGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAAc > 1:77961/1‑241 (MQ=255) accgggcgcgcgCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTacatgc > 1:863325/9‑193 (MQ=255) ttAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATc < 1:284332/251‑1 (MQ=255) aaaaTCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGc < 1:16269/251‑1 (MQ=255) aaaTCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGAc > 1:875800/1‑193 (MQ=255) aaaTCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCa > 1:842762/1‑251 (MQ=255) aaTCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCAt < 1:9621/251‑1 (MQ=255) ggctaTGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAAc > 1:245688/5‑200 (MQ=255) ggctaTGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCt > 1:607405/5‑250 (MQ=255) ggCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGt < 1:414075/227‑1 (MQ=255) ggCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCaga > 1:816676/1‑248 (MQ=255) ggCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCag > 1:72784/1‑248 (MQ=255) gCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACAtgcgcgc > 1:178253/1‑217 (MQ=255) gCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCaga > 1:512315/1‑247 (MQ=255) aCCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAatat < 1:120957/250‑1 (MQ=255) gATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATatttattt > 1:491882/1‑251 (MQ=255) gACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAatat < 1:630242/239‑1 (MQ=255) tGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCaagaa > 1:700330/1‑138 (MQ=255) tGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTgcac < 1:804415/169‑3 (MQ=255) tGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCAt > 1:561728/1‑200 (MQ=255) cagcaCCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAAt < 1:371999/250‑1 (MQ=255) gcaCCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATc < 1:72145/154‑1 (MQ=255) gcaCCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTActgc > 1:185231/1‑210 (MQ=255) aTCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAagc < 1:276096/251‑2 (MQ=255) cAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGAtt < 1:342797/249‑1 (MQ=255) cTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATcc < 1:714261/158‑1 (MQ=255) cTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATcc < 1:641055/218‑1 (MQ=255) tttttACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTat > 1:661828/1‑250 (MQ=255) ttttACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCaagaag < 1:213697/99‑1 (MQ=255) ttttACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTatc < 1:389396/251‑3 (MQ=255) gtgGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAACCAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGt > 1:518286/1‑251 (MQ=255) gtgGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTACCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGt > 1:438555/1‑251 (MQ=255) atatGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAAc < 1:95731/250‑1 (MQ=255) cAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAAcc > 1:211575/1‑224 (MQ=255) accgATATGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAAttgt > 1:365137/4‑251 (MQ=255) atatGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTc < 1:342916/152‑1 (MQ=255) atatGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCCGTCCGTTAACCCTTTTTCAAAATGAAATTGTTGaa > 1:643498/1‑251 (MQ=255) atatGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGaa > 1:774546/1‑251 (MQ=255) atatGATACTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCATGTGAAt > 1:435339/1‑177 (MQ=255) atacTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAAt < 1:250877/159‑1 (MQ=255) atacTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGAtt > 1:277133/3‑199 (MQ=255) atacTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATg > 1:894784/3‑250 (MQ=255) atacTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATg > 1:316817/3‑250 (MQ=255) atacTGACCTATGCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATg > 1:223346/3‑250 (MQ=255) tgacctatgCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACt < 1:737168/243‑1 (MQ=255) gacctatgCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAAtatttatt < 1:292839/145‑1 (MQ=255) gacctatgCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATgg > 1:278334/8‑158 (MQ=255) gacctatgCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTatc > 1:670919/8‑203 (MQ=255) gacctatgCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTc > 1:411756/8‑224 (MQ=255) gacctatgCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACtt > 1:495585/8‑251 (MQ=255) atgCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGcc < 1:209195/249‑1 (MQ=255) atgCCAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGcc < 1:421074/249‑1 (MQ=255) cAATCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGtg < 1:5759/251‑1 (MQ=255) tCTATTACGCTTACATGCCGGTTTTGATGAAACTCAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGtgatt > 1:517494/1‑251 (MQ=255) | GCTTTGCCCCATTTGACCGTAACACGCACCGATTTAATCCATTGGATTGTATTGATATGCGCGACCGAGCACGCGCCGAACTCGATTTAAAAAATCAGGCAATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAAAATCTTTTTGTGGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACTGATATGACACTGACTTACGCCAATCTACTACGTTTACACGCAGGTTTTGACGAAACGCAAGAAGACGGGTCAATAGAACATATCGATTTTGCGCAACACGCGCGGTTATGCGTTCAATCCGGCATTGCTCACCAAACTACCGCCGATAAACTCAATATTTATTTAATGGCGTGCGAATCCGGTAAAACAAAAAGTAGTATCGATTCAACTTTTACCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAACATGCGACTTCTGCCAGTGATT > 1/1453327‑1453818 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |