Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,453,337	A→G	P180P (CCA→CCG)	unknown →	Coupling protein VirD4, ATPase required for T‑DNA transfer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,453,337	0	A	G	100.0%	146.2 / NA	44	P180P (CCA→CCG)	unknown	Coupling protein VirD4, ATPase required for T‑DNA transfer
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/25);  total (19/25)

TACCGAATTTATTAGAGTGGCAACAATCAGTTGTTGTGAAAGATGTTAAAAACGAATGTTATCGCATTACCAGTAAATACCGTCAAAAAATATTGGGGCAAAAAGTCTATCGCTTTGCCCCATTTGACCGTAACACGCACCGATTTAATCCATTGGATTGTATTGATATGCGCGACCGAGCACGCGCCGAACTCGATTTAAAAAATCAGGCAATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAAAATCTTTTTGTGGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACTGATATGACACTGACTTACGCCAATCTACTACG  >  1/1453216‑1453576                                                                                                                          |                                                                                                                                                                                                                                                ttccaaatttgctggcatgggaacaaTCTGTGGTTGTGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGAc                                                                                                                <  1:658709/230‑1 (MQ=255)                catgggaacaaTCTGTGGTTGTGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATc                                                                                                                                          >  1:761916/7‑210 (MQ=255)                     gaacaaTCTGTGGTTGTGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGcac                                                                                             <  1:601535/249‑1 (MQ=255)                          atctgtggttgtGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTatat                                                                                                                                           >  1:709998/8‑199 (MQ=255)                          atctgtggttgtGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAAt                                                                                        >  1:407013/8‑250 (MQ=255)                              gtggttgtGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCtttt                                                                                   >  1:222724/4‑251 (MQ=255)                                 gttgtGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGAt                                                                                                                      <  1:489360/213‑1 (MQ=255)                                 gttgtGAAGGACATTAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTtgt                                                                                >  1:463565/1‑251 (MQ=255)                                   tgtgaaggacattAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTtgtag                                                                              <  1:636583/240‑3 (MQ=255)                                    gtgaaggacattAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATc                                                                                                                                          >  1:177614/11‑190 (MQ=255)                                            cattAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGc                                                                                                                                                 >  1:630866/3‑175 (MQ=255)                                            cattAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATc                                                                                                                                          >  1:353291/3‑182 (MQ=255)                                            cattAAAAACGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACtt                                                                     >  1:795126/3‑251 (MQ=255)                                                  aaaCGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTAc                                                               <  1:779473/251‑1 (MQ=255)                                                  aaaCGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTAc                                                               <  1:241414/251‑1 (MQ=255)                                                  aaaCGAATGCTATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTAc                                                               <  1:806529/251‑1 (MQ=255)                                                        atgctATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTatat                                                                                                                                           <  1:715876/165‑1 (MQ=255)                                                           ctATCGTATCACCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGata                                                      >  1:876069/2‑251 (MQ=255)                                                               cgtatcaCCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTatat                                                                                                                                           <  1:40005/156‑1 (MQ=255)                                                               cgtatcaCCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTAc                                                               <  1:864890/232‑1 (MQ=255)                                                               cgtatcaCCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATgcg                                                  >  1:170613/7‑251 (MQ=255)                                                                 tatcaCCAGTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATgcgc                                                 <  1:796497/246‑1 (MQ=255)                                                                         gTAAATATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTAAATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAg                                         >  1:119774/1‑250 (MQ=255)                                                                           aaaTATCGACAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGc                                        >  1:356409/1‑249 (MQ=255)                                                                            aatatcgacAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCt                                                                                                                                                <  1:63099/136‑1 (MQ=255)                                                                                tcgacAAAAAATATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTAccg                                 <  1:84567/247‑3 (MQ=255)                                                                                        aaaTATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACTCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTAc                                                               <  1:157528/213‑1 (MQ=255)                                                                                        aaaTATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGata                         >  1:286276/1‑249 (MQ=255)                                                                                         aaTATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGatac                        <  1:300752/251‑4 (MQ=255)                                                                                         aaTATTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGatac                        <  1:897884/251‑4 (MQ=255)                                                                                          atatTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGatac                        <  1:411677/250‑4 (MQ=255)                                                                                          atatTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGata                         >  1:858618/1‑247 (MQ=255)                                                                                           tatTAGGGCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGatac                        <  1:772374/249‑4 (MQ=255)                                                                                              tagggCAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGAcc                   <  1:368887/249‑2 (MQ=255)                                                                                                   cAAAAGGTATATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGAcctatgc              <  1:158817/251‑7 (MQ=255)                                                                                                    aaaaggtatATCGCTTTGCCCCGTTTGACCGTAATACGCGCCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGAcctatgcc             <  1:733331/243‑8 (MQ=255)                                                                                                    aaaaggtatATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGAcctatgcc             <  1:162708/243‑8 (MQ=255)                                                                                                       gcgatataccttttGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAAt          >  1:691873/12‑251 (MQ=255)                                                                                                         gtatATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAAt          >  1:752920/4‑249 (MQ=255)                                                                                                         gtatATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATc         <  1:750716/247‑1 (MQ=255)                                                                                                         gtatATCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCACCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATc         <  1:888394/247‑1 (MQ=255)                                                                                                             aTCGCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATctatta    <  1:21302/251‑4 (MQ=255)                                                                                                                gCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGAcc                   >  1:302181/1‑232 (MQ=255)                                                                                                                gCTTTGCCCCGTTTGACCGTAATACGCACCGGTTTAATCCGCTGGATTGTATTGATATGCGCGACCGGGCGCGCGCCGAACTCGATTTAAAAAATCAGGCTATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAGAATCTTTTTGTAGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACCGATATGATACTGACCTATGCCAATCTATTACg  >  1:176680/1‑250 (MQ=255)                                                                                                                          |                                                                                                                                                                                                                                                TACCGAATTTATTAGAGTGGCAACAATCAGTTGTTGTGAAAGATGTTAAAAACGAATGTTATCGCATTACCAGTAAATACCGTCAAAAAATATTGGGGCAAAAAGTCTATCGCTTTGCCCCATTTGACCGTAACACGCACCGATTTAATCCATTGGATTGTATTGATATGCGCGACCGAGCACGCGCCGAACTCGATTTAAAAAATCAGGCAATGGGCTTATATCCGCTCACCGGCGACCCGGATAAAGACTTTTGGCAGCACCAGGCACAAAATCTTTTTGTGGCAACCACTTTTTTACTGTGGGATATGCGCCAAAAAAGCCTTACTGATATGACACTGACTTACGCCAATCTACTACG  >  1/1453216‑1453576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑