Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,452,496	C→T	L245L (CTG→TTA)	unknown →	ATPase required for both assembly of type IV secretion complex and secretion of T‑DNA complex, VirB11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,452,496	0	C	T	100.0%	289.8 / NA	86	L245L (CTG→TTA)	unknown	ATPase required for both assembly of type IV secretion complex and secretion of T‑DNA complex, VirB11
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (53/33);  total (53/33)

ATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTGGTCGATTATATTCCGTTGCATGAACGCCTAGGCACCATCGAAGATACTCCGGAATTATTCTTACGCCAACATAAGAATTATTTCCACTTATTCTATCCAAGTGAAGCAAAAAACGGCGATTTAATCACAGCAGCCTCTTTGCTAAAATCCTGCTTACGGATGAAGCCTGATCGTATTCTGCTTGCGGAATTACGCGGTGGGGAAACTTATGATTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCACCGTTTATTACAACAAACGATAGATATTATCGTGCATGTTACCAACAGTCACGAATACGGCAGACA  >  1/1452263‑1452737                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   atatTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTAcga                                                                                                                                                                                                                                   >  1:255552/1‑250 (MQ=255)                  gAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGa                                                                                                                                                                                                                  >  1:608713/1‑251 (MQ=255)                             cGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAAtg                                                                                                                                                                                                       <  1:323651/251‑1 (MQ=255)                              gggAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAAc                                                                                                                                                                                                                        >  1:325609/1‑233 (MQ=255)                                        cTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTttcttc                                                                                                                                                                                             >  1:263081/1‑250 (MQ=255)                                            aaTGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGc                                                                                                                                                                                        <  1:119083/251‑1 (MQ=255)                                                   gCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACgg                                                                                                                                                                                 >  1:520970/1‑251 (MQ=255)                                                    cccTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGac                                                                                                                                                                                                                 >  1:104685/1‑217 (MQ=255)                                                        agTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCa                                                                                                                                                                                       >  1:405645/2‑240 (MQ=255)                                                           cGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAAc                                                                                                                                                                                                                        >  1:620577/1‑204 (MQ=255)                                                           cGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGAGGTTTCTTCCGGGCATAACGGGTCGATTa                                                                                                                                                                         >  1:642640/1‑251 (MQ=255)                                                             aTTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATc                                                                                                                                                                                                           >  1:372620/1‑215 (MQ=255)                                                             aTTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAAtgt                                                                                                                                                                                                      <  1:741558/220‑1 (MQ=255)                                                                atatTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACg                                                                                                                                                                                  >  1:415635/1‑237 (MQ=255)                                                                  atTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTg                                                                                                                                                                  >  1:61451/1‑251 (MQ=255)                                                                  atTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTg                                                                                                                                                                  >  1:356742/1‑251 (MQ=255)                                                                                 cGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCcggttcggt                                                                                                                                                  >  1:297145/1‑251 (MQ=255)                                                                                   cctggggaCCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGGTTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTg                                                                                                                                                >  1:316054/8‑251 (MQ=255)                                                                                   cctggggaCCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTg                                                                                                                                                >  1:421188/8‑251 (MQ=255)                                                                                        ggaCCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGGATTTTACTTGCGGAATTACGCGGGGGGGGAACTTTTGACTTTTTCCATGTGGGTTCTTTCCGGCATAAAGGGGTGATTACCTCTTTTCATGGCCGG‑TCGGGTGCTgga                                                                                                                                           >  1:72987/3‑249 (MQ=255)                                                                                          aCCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAAc                                                                                                                                         <  1:808223/251‑1 (MQ=255)                                                                                           ccATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGttt                                                                                                                                                                                                 <  1:302294/195‑1 (MQ=255)                                                                                           ccATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTttcttc                                                                                                                                                                                             >  1:672474/1‑199 (MQ=255)                                                                                             attgAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAAc                                                                                                                                                                                   <  1:877432/204‑1 (MQ=255)                                                                                                gAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGa                                                                                                                                   >  1:189599/1‑251 (MQ=255)                                                                                                       ccccGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAAc                                                                                                                                                                                                                        <  1:729681/158‑1 (MQ=255)                                                                                                       ccccGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCtt                                                                                                                            >  1:28290/3‑251 (MQ=255)                                                                                                            gAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTAtttt                                                                                                                       >  1:45976/1‑251 (MQ=255)                                                                                                            gAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTAtttt                                                                                                                       >  1:830723/1‑251 (MQ=255)                                                                                                                          ccAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCaa                                                                                                          <  1:218333/250‑1 (MQ=255)                                                                                                                             aCATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGa                                                                                                                  <  1:134641/239‑1 (MQ=255)                                                                                                                              cATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTAtttt                                                                                                                       >  1:823741/1‑233 (MQ=255)                                                                                                                              cATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCaa                                                                                                          <  1:745124/246‑1 (MQ=255)                                                                                                                              cATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGa                                                                                                     >  1:354/1‑251 (MQ=255)                                                                                                                              cATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGa                                                                                                     >  1:705455/1‑251 (MQ=255)                                                                                                                                     actattTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAggg                                                                                              <  1:244473/249‑1 (MQ=255)                                                                                                                                      ctattTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAggg                                                                                              >  1:706465/2‑250 (MQ=255)                                                                                                                                      ctattTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAggg                                                                                              >  1:710726/2‑250 (MQ=255)                                                                                                                                            ccACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACg                                                                                        >  1:130820/1‑250 (MQ=255)                                                                                                                                             cACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACg                                                                                        >  1:547664/1‑249 (MQ=255)                                                                                                                                             cACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGc                                                                                       >  1:360948/1‑250 (MQ=255)                                                                                                                                                 tATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAAt                                                                                                                                                                                                        <  1:465574/134‑1 (MQ=255)                                                                                                                                                 tATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTAc                                                                                                                                                                        >  1:476172/1‑166 (MQ=255)                                                                                                                                                 tATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTc                                                                                                                                                                >  1:44450/1‑174 (MQ=255)                                                                                                                                                 tATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAAc                                                                                                                                 >  1:738658/1‑204 (MQ=255)                                                                                                                                                 tATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTc                                                                                   >  1:128961/1‑250 (MQ=255)                                                                                                                                                  aTTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTc                                                                                   >  1:723054/1‑249 (MQ=255)                                                                                                                                                  aTTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCAGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTAtttt                                                                                                                       >  1:82153/1‑213 (MQ=255)                                                                                                                                                   ttCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTa                                                                                <  1:42268/251‑1 (MQ=255)                                                                                                                                                   ttCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTa                                                                                <  1:164599/251‑1 (MQ=255)                                                                                                                                                    tCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTat                                                                               <  1:668492/251‑1 (MQ=255)                                                                                                                                                    tCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTat                                                                               <  1:10369/251‑1 (MQ=255)                                                                                                                                                     cTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTAtttt                                                                                                                       >  1:644618/1‑210 (MQ=255)                                                                                                                                                      tATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTatga                                                                             <  1:689190/251‑1 (MQ=255)                                                                                                                                                      tATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTataa                                                                             <  1:74041/251‑3 (MQ=255)                                                                                                                                                       aTCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTa                                                                                                                                                                         >  1:321158/1‑159 (MQ=255)                                                                                                                                                       aTCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGa                                                                                                     >  1:784712/1‑226 (MQ=255)                                                                                                                                                       aTCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTatgat                                                                            >  1:888109/1‑251 (MQ=255)                                                                                                                                                       aTCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTCGCAAAACGACCAAGGGCGCACGCTTTCCTatgat                                                                            >  1:319702/1‑251 (MQ=255)                                                                                                                                                        tCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTatgatg                                                                           <  1:838732/251‑1 (MQ=255)                                                                                                                                                             gcgAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAAtt                                                                      >  1:645792/3‑251 (MQ=255)                                                                                                                                                                aaGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTatga                                                                             <  1:843960/241‑1 (MQ=255)                                                                                                                                                                aaGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCAGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTcg                                                                    >  1:437426/1‑249 (MQ=255)                                                                                                                                                                    aaaaaaTGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGtt                                                               >  1:125851/1‑251 (MQ=255)                                                                                                                                                                          tggtgATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGCTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTac                                                         <  1:74814/247‑1 (MQ=255)                                                                                                                                                                              gATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTatga                                                                             <  1:608583/227‑1 (MQ=255)                                                                                                                                                                              gATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTat                                                                               >  1:672766/1‑225 (MQ=255)                                                                                                                                                                                   aaTCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGa                                                                                                                  <  1:234638/185‑1 (MQ=255)                                                                                                                                                                                   aaTCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACga                                                >  1:797534/1‑251 (MQ=255)                                                                                                                                                                                   aaTCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTAACTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTat                                                                               >  1:644556/1‑220 (MQ=255)                                                                                                                                                                                        cggctgcCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTacaa                                                       <  1:323811/234‑1 (MQ=255)                                                                                                                                                                                          gctgcCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACgatagata                                          <  1:35344/247‑1 (MQ=255)                                                                                                                                                                                           ctgcCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGAtatt                                        <  1:809259/249‑1 (MQ=255)                                                                                                                                                                                              ccTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGAtattat                                      <  1:716255/250‑1 (MQ=255)                                                                                                                                                                                                 ctTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTCATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGAtattattgtt                                  >  1:537164/1‑247 (MQ=255)                                                                                                                                                                                                    tGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACgatagata                                          >  1:6080/1‑240 (MQ=255)                                                                                                                                                                                                     gctgaaaTCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATg                              >  1:564100/5‑251 (MQ=255)                                                                                                                                                                                                     gctgaaaTCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCCTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATg                              >  1:323443/5‑251 (MQ=255)                                                                                                                                                                                                               tgcctACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAg                    <  1:329905/247‑1 (MQ=255)                                                                                                                                                                                                                       aaTGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGaac            <  1:84306/250‑2 (MQ=255)                                                                                                                                                                                                                       aaTGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGaac            <  1:460419/250‑2 (MQ=255)                                                                                                                                                                                                                       aaTGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGaac            <  1:566459/250‑2 (MQ=255)                                                                                                                                                                                                                       aaTGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCACAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGaac            <  1:440877/250‑2 (MQ=255)                                                                                                                                                                                                                             accggATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGaac            >  1:660126/5‑244 (MQ=255)                                                                                                                                                                                                                                 gATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGaac            >  1:390308/1‑240 (MQ=255)                                                                                                                                                                                                                                  aTCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACa  <  1:846655/250‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   ATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTGGTCGATTATATTCCGTTGCATGAACGCCTAGGCACCATCGAAGATACTCCGGAATTATTCTTACGCCAACATAAGAATTATTTCCACTTATTCTATCCAAGTGAAGCAAAAAACGGCGATTTAATCACAGCAGCCTCTTTGCTAAAATCCTGCTTACGGATGAAGCCTGATCGTATTCTGCTTGCGGAATTACGCGGTGGGGAAACTTATGATTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCACCGTTTATTACAACAAACGATAGATATTATCGTGCATGTTACCAACAGTCACGAATACGGCAGACA  >  1/1452263‑1452737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑