Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,452,483	G→A	K240K (AAG→AAA)	unknown →	ATPase required for both assembly of type IV secretion complex and secretion of T‑DNA complex, VirB11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,452,483	0	G	A	100.0%	293.3 / NA	88	K240K (AAG→AAA)	unknown	ATPase required for both assembly of type IV secretion complex and secretion of T‑DNA complex, VirB11
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (53/35);  total (53/35)

CATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTGGTCGATTATATTCCGTTGCATGAACGCCTAGGCACCATCGAAGATACTCCGGAATTATTCTTACGCCAACATAAGAATTATTTCCACTTATTCTATCCAAGTGAAGCAAAAAACGGCGATTTAATCACAGCAGCCTCTTTGCTAAAATCCTGCTTACGGATGAAGCCTGATCGTATTCTGCTTGCGGAATTACGCGGTGGGGAAACTTATGATTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCACCGTTTATTACAACAAACGATAGATATTATCGTGCATGTTACCAACAGTCACGAAT  >  1/1452249‑1452727                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      cATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTAttttac                                                                                                                                                                                                                                       <  1:714019/251‑5 (MQ=255) cATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTAttttac                                                                                                                                                                                                                                       <  1:809806/251‑5 (MQ=255)               atatTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTAcga                                                                                                                                                                                                                         >  1:255552/1‑250 (MQ=255)                    gtgATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTAttttactggcg                                                                                                                                                                                                                                  >  1:166111/1‑228 (MQ=255)                                gAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGa                                                                                                                                                                                                        >  1:608713/1‑251 (MQ=255)                                           cGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAAtg                                                                                                                                                                                             <  1:323651/251‑1 (MQ=255)                                            gggAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAAc                                                                                                                                                                                                              >  1:325609/1‑233 (MQ=255)                                                      cTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTttcttc                                                                                                                                                                                   >  1:263081/1‑250 (MQ=255)                                                          aaTGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGc                                                                                                                                                                              <  1:119083/251‑1 (MQ=255)                                                                 gCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACgg                                                                                                                                                                       >  1:520970/1‑251 (MQ=255)                                                                  cccTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGac                                                                                                                                                                                                       >  1:104685/1‑217 (MQ=255)                                                                      agTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCa                                                                                                                                                                             >  1:405645/2‑240 (MQ=255)                                                                         cGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAAc                                                                                                                                                                                                              >  1:620577/1‑204 (MQ=255)                                                                         cGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGAGGTTTCTTCCGGGCATAACGGGTCGATTa                                                                                                                                                               >  1:642640/1‑251 (MQ=255)                                                                           aTTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTAttttac                                                                                                                                                                                                                                       <  1:205032/177‑5 (MQ=255)                                                                           aTTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATc                                                                                                                                                                                                 >  1:372620/1‑215 (MQ=255)                                                                           aTTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAAtgt                                                                                                                                                                                            <  1:741558/220‑1 (MQ=255)                                                                              atatTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACg                                                                                                                                                                        >  1:415635/1‑237 (MQ=255)                                                                                atTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTg                                                                                                                                                        >  1:356742/1‑251 (MQ=255)                                                                                atTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTg                                                                                                                                                        >  1:61451/1‑251 (MQ=255)                                                                                               cGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCcggttcggt                                                                                                                                        >  1:297145/1‑251 (MQ=255)                                                                                                 cctggggaCCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGGTTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTg                                                                                                                                      >  1:316054/8‑251 (MQ=255)                                                                                                 cctggggaCCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTAttttac                                                                                                                                                                                                                                       >  1:75971/8‑151 (MQ=255)                                                                                                 cctggggaCCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTg                                                                                                                                      >  1:421188/8‑251 (MQ=255)                                                                                                      ggaCCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGGATTTTACTTGCGGAATTACGCGGGGGGGGAACTTTTGACTTTTTCCATGTGGGTTCTTTCCGGCATAAAGGGGTGATTACCTCTTTTCATGGCCGG‑TCGGGTGCTgga                                                                                                                                 >  1:72987/3‑249 (MQ=255)                                                                                                        aCCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAAc                                                                                                                               <  1:808223/251‑1 (MQ=255)                                                                                                         ccATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGttt                                                                                                                                                                                       <  1:302294/195‑1 (MQ=255)                                                                                                         ccATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTttcttc                                                                                                                                                                                   >  1:672474/1‑199 (MQ=255)                                                                                                           attgAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAAc                                                                                                                                                                         <  1:877432/204‑1 (MQ=255)                                                                                                              gAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGa                                                                                                                         >  1:189599/1‑251 (MQ=255)                                                                                                                     ccccGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAAc                                                                                                                                                                                                              <  1:729681/158‑1 (MQ=255)                                                                                                                     ccccGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCtt                                                                                                                  >  1:28290/3‑251 (MQ=255)                                                                                                                          gAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTAtttt                                                                                                             >  1:45976/1‑251 (MQ=255)                                                                                                                          gAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTAtttt                                                                                                             >  1:830723/1‑251 (MQ=255)                                                                                                                                        ccAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCaa                                                                                                <  1:218333/250‑1 (MQ=255)                                                                                                                                           aCATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGa                                                                                                        <  1:134641/239‑1 (MQ=255)                                                                                                                                            cATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTAtttt                                                                                                             >  1:823741/1‑233 (MQ=255)                                                                                                                                            cATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCaa                                                                                                <  1:745124/246‑1 (MQ=255)                                                                                                                                            cATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGa                                                                                           >  1:705455/1‑251 (MQ=255)                                                                                                                                            cATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGa                                                                                           >  1:354/1‑251 (MQ=255)                                                                                                                                                   actattTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAggg                                                                                    <  1:244473/249‑1 (MQ=255)                                                                                                                                                    ctattTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAggg                                                                                    >  1:706465/2‑250 (MQ=255)                                                                                                                                                    ctattTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAggg                                                                                    >  1:710726/2‑250 (MQ=255)                                                                                                                                                          ccACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACg                                                                              >  1:130820/1‑250 (MQ=255)                                                                                                                                                           cACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACg                                                                              >  1:547664/1‑249 (MQ=255)                                                                                                                                                           cACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGc                                                                             >  1:360948/1‑250 (MQ=255)                                                                                                                                                               tATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAAt                                                                                                                                                                                              <  1:465574/134‑1 (MQ=255)                                                                                                                                                               tATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTAc                                                                                                                                                              >  1:476172/1‑166 (MQ=255)                                                                                                                                                               tATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTc                                                                                                                                                      >  1:44450/1‑174 (MQ=255)                                                                                                                                                               tATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAAc                                                                                                                       >  1:738658/1‑204 (MQ=255)                                                                                                                                                               tATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTc                                                                         >  1:128961/1‑250 (MQ=255)                                                                                                                                                                aTTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTc                                                                         >  1:723054/1‑249 (MQ=255)                                                                                                                                                                aTTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCAGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTAtttt                                                                                                             >  1:82153/1‑213 (MQ=255)                                                                                                                                                                 ttCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTa                                                                      <  1:42268/251‑1 (MQ=255)                                                                                                                                                                 ttCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTa                                                                      <  1:164599/251‑1 (MQ=255)                                                                                                                                                                  tCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTat                                                                     <  1:10369/251‑1 (MQ=255)                                                                                                                                                                  tCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTat                                                                     <  1:668492/251‑1 (MQ=255)                                                                                                                                                                   cTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTAtttt                                                                                                             >  1:644618/1‑210 (MQ=255)                                                                                                                                                                    tATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTatga                                                                   <  1:689190/251‑1 (MQ=255)                                                                                                                                                                    tATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTataa                                                                   <  1:74041/251‑3 (MQ=255)                                                                                                                                                                     aTCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTa                                                                                                                                                               >  1:321158/1‑159 (MQ=255)                                                                                                                                                                     aTCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGa                                                                                           >  1:784712/1‑226 (MQ=255)                                                                                                                                                                     aTCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTatgat                                                                  >  1:888109/1‑251 (MQ=255)                                                                                                                                                                     aTCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTCGCAAAACGACCAAGGGCGCACGCTTTCCTatgat                                                                  >  1:319702/1‑251 (MQ=255)                                                                                                                                                                      tCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTatgatg                                                                 <  1:838732/251‑1 (MQ=255)                                                                                                                                                                           gcgAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAAtt                                                            >  1:645792/3‑251 (MQ=255)                                                                                                                                                                              aaGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTatga                                                                   <  1:843960/241‑1 (MQ=255)                                                                                                                                                                              aaGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCAGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTcg                                                          >  1:437426/1‑249 (MQ=255)                                                                                                                                                                                  aaaaaaTGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGtt                                                     >  1:125851/1‑251 (MQ=255)                                                                                                                                                                                        tggtgATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGCTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTac                                               <  1:74814/247‑1 (MQ=255)                                                                                                                                                                                            gATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTatga                                                                   <  1:608583/227‑1 (MQ=255)                                                                                                                                                                                            gATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTat                                                                     >  1:672766/1‑225 (MQ=255)                                                                                                                                                                                                 aaTCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGa                                                                                                        <  1:234638/185‑1 (MQ=255)                                                                                                                                                                                                 aaTCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACga                                      >  1:797534/1‑251 (MQ=255)                                                                                                                                                                                                 aaTCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTAACTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTat                                                                     >  1:644556/1‑220 (MQ=255)                                                                                                                                                                                                      cggctgcCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTacaa                                             <  1:323811/234‑1 (MQ=255)                                                                                                                                                                                                        gctgcCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACgatagata                                <  1:35344/247‑1 (MQ=255)                                                                                                                                                                                                         ctgcCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGAtatt                              <  1:809259/249‑1 (MQ=255)                                                                                                                                                                                                            ccTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGAtattat                            <  1:716255/250‑1 (MQ=255)                                                                                                                                                                                                               ctTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTCATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGAtattattgtt                        >  1:537164/1‑247 (MQ=255)                                                                                                                                                                                                                  tGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACgatagata                                >  1:6080/1‑240 (MQ=255)                                                                                                                                                                                                                   gctgaaaTCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATg                    >  1:564100/5‑251 (MQ=255)                                                                                                                                                                                                                   gctgaaaTCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCCTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATg                    >  1:323443/5‑251 (MQ=255)                                                                                                                                                                                                                             tgcctACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAg          <  1:329905/247‑1 (MQ=255)                                                                                                                                                                                                                                     aaTGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGaac  <  1:460419/250‑2 (MQ=255)                                                                                                                                                                                                                                     aaTGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGaac  <  1:84306/250‑2 (MQ=255)                                                                                                                                                                                                                                     aaTGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGaac  <  1:566459/250‑2 (MQ=255)                                                                                                                                                                                                                                     aaTGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCACAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGaac  <  1:440877/250‑2 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      CATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTGGTCGATTATATTCCGTTGCATGAACGCCTAGGCACCATCGAAGATACTCCGGAATTATTCTTACGCCAACATAAGAATTATTTCCACTTATTCTATCCAAGTGAAGCAAAAAACGGCGATTTAATCACAGCAGCCTCTTTGCTAAAATCCTGCTTACGGATGAAGCCTGATCGTATTCTGCTTGCGGAATTACGCGGTGGGGAAACTTATGATTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCACCGTTTATTACAACAAACGATAGATATTATCGTGCATGTTACCAACAGTCACGAAT  >  1/1452249‑1452727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑