Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,452,447	A→G	T228T (ACA→ACG)	unknown →	ATPase required for both assembly of type IV secretion complex and secretion of T‑DNA complex, VirB11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,452,447	0	A	G	100.0%	278.0 / NA	83	T228T (ACA→ACG)	unknown	ATPase required for both assembly of type IV secretion complex and secretion of T‑DNA complex, VirB11
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (56/27);  total (56/27)

TCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTGGTCGATTATATTCCGTTGCATGAACGCCTAGGCACCATCGAAGATACTCCGGAATTATTCTTACGCCAACATAAGAATTATTTCCACTTATTCTATCCAAGTGAAGCAAAAAACGGCGATTTAATCACAGCAGCCTCTTTGCTAAAATCCTGCTTACGGATGAAGCCTGATCGTATTCTGCTTGCGGAATTACGCGGTGGGGAAACTTATGATTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCACCGTTTATTACAACAAACGA  >  1/1452210‑1452691                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      tCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTg                                                                                                                                                                                                                                           >  1:806088/1‑250 (MQ=255)     aaGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACACCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCtgaa                                                                                                                                                                                                                                      >  1:437037/1‑248 (MQ=255)            aTTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATcttg                                                                                                                                                                                                                                >  1:420622/1‑247 (MQ=255)                        tCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGaaa                                                                                                                                                                                                                   >  1:637024/1‑250 (MQ=255)                        tCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGaaa                                                                                                                                                                                                                   >  1:830234/1‑250 (MQ=255)                         ccTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGaaa                                                                                                                                                                                                                   >  1:153121/1‑249 (MQ=255)                         ccTTAAACAAGCGGTCATTTGCGGGAAAAATATTGAGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGaaa                                                                                                                                                                                                                   >  1:614619/1‑249 (MQ=255)                          cTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAATCCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGaaacc                                                                                                                                                                                                                 <  1:682227/251‑4 (MQ=255)                                        cATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTAttttac                                                                                                                                                                                                   <  1:714019/251‑5 (MQ=255)                                        cATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTAttttac                                                                                                                                                                                                   <  1:809806/251‑5 (MQ=255)                                                      atatTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTAcga                                                                                                                                                                                     >  1:255552/1‑250 (MQ=255)                                                           gtgATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTAttttactggcg                                                                                                                                                                                              >  1:166111/1‑228 (MQ=255)                                                                       gAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGa                                                                                                                                                                    >  1:608713/1‑251 (MQ=255)                                                                                  cGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAAtg                                                                                                                                                         <  1:323651/251‑1 (MQ=255)                                                                                   gggAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAAc                                                                                                                                                                          >  1:325609/1‑233 (MQ=255)                                                                                             cTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTttcttc                                                                                                                                               >  1:263081/1‑250 (MQ=255)                                                                                                 aaTGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGc                                                                                                                                          <  1:119083/251‑1 (MQ=255)                                                                                                        gCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACgg                                                                                                                                   >  1:520970/1‑251 (MQ=255)                                                                                                         cccTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGac                                                                                                                                                                   >  1:104685/1‑217 (MQ=255)                                                                                                             agTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCa                                                                                                                                         >  1:405645/2‑240 (MQ=255)                                                                                                                cGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAAc                                                                                                                                                                          >  1:620577/1‑204 (MQ=255)                                                                                                                cGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGAGGTTTCTTCCGGGCATAACGGGTCGATTa                                                                                                                           >  1:642640/1‑251 (MQ=255)                                                                                                                  aTTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTAttttac                                                                                                                                                                                                   <  1:205032/177‑5 (MQ=255)                                                                                                                  aTTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATc                                                                                                                                                             >  1:372620/1‑215 (MQ=255)                                                                                                                  aTTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAAtgt                                                                                                                                                        <  1:741558/220‑1 (MQ=255)                                                                                                                     atatTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACg                                                                                                                                    >  1:415635/1‑237 (MQ=255)                                                                                                                       atTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTg                                                                                                                    >  1:356742/1‑251 (MQ=255)                                                                                                                       atTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTg                                                                                                                    >  1:61451/1‑251 (MQ=255)                                                                                                                                      cGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCcggttcggt                                                                                                    >  1:297145/1‑251 (MQ=255)                                                                                                                                        cctggggaCCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGGTTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTg                                                                                                  >  1:316054/8‑251 (MQ=255)                                                                                                                                        cctggggaCCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTAttttac                                                                                                                                                                                                   >  1:75971/8‑151 (MQ=255)                                                                                                                                        cctggggaCCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTg                                                                                                  >  1:421188/8‑251 (MQ=255)                                                                                                                                             ggaCCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGGATTTTACTTGCGGAATTACGCGGGGGGGGAACTTTTGACTTTTTCCATGTGGGTTCTTTCCGGCATAAAGGGGTGATTACCTCTTTTCATGGCCGG‑TCGGGTGCTgga                                                                                             >  1:72987/3‑249 (MQ=255)                                                                                                                                               aCCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAAc                                                                                           <  1:808223/251‑1 (MQ=255)                                                                                                                                                ccATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGttt                                                                                                                                                   <  1:302294/195‑1 (MQ=255)                                                                                                                                                ccATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTttcttc                                                                                                                                               >  1:672474/1‑199 (MQ=255)                                                                                                                                                  attgAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAAc                                                                                                                                     <  1:877432/204‑1 (MQ=255)                                                                                                                                                     gAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGa                                                                                     >  1:189599/1‑251 (MQ=255)                                                                                                                                                            ccccGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAAc                                                                                                                                                                          <  1:729681/158‑1 (MQ=255)                                                                                                                                                            ccccGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCtt                                                                              >  1:28290/3‑251 (MQ=255)                                                                                                                                                                 gAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTAtttt                                                                         >  1:45976/1‑251 (MQ=255)                                                                                                                                                                 gAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTAtttt                                                                         >  1:830723/1‑251 (MQ=255)                                                                                                                                                                               ccAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCaa                                                            <  1:218333/250‑1 (MQ=255)                                                                                                                                                                                  aCATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGa                                                                    <  1:134641/239‑1 (MQ=255)                                                                                                                                                                                   cATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTAtttt                                                                         >  1:823741/1‑233 (MQ=255)                                                                                                                                                                                   cATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCaa                                                            <  1:745124/246‑1 (MQ=255)                                                                                                                                                                                   cATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGa                                                       >  1:705455/1‑251 (MQ=255)                                                                                                                                                                                   cATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGa                                                       >  1:354/1‑251 (MQ=255)                                                                                                                                                                                          actattTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAggg                                                <  1:244473/249‑1 (MQ=255)                                                                                                                                                                                           ctattTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAggg                                                >  1:710726/2‑250 (MQ=255)                                                                                                                                                                                           ctattTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAggg                                                >  1:706465/2‑250 (MQ=255)                                                                                                                                                                                                 ccACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACg                                          >  1:130820/1‑250 (MQ=255)                                                                                                                                                                                                  cACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACg                                          >  1:547664/1‑249 (MQ=255)                                                                                                                                                                                                  cACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGc                                         >  1:360948/1‑250 (MQ=255)                                                                                                                                                                                                      tATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAAt                                                                                                                                                          <  1:465574/134‑1 (MQ=255)                                                                                                                                                                                                      tATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTAc                                                                                                                          >  1:476172/1‑166 (MQ=255)                                                                                                                                                                                                      tATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTc                                                                                                                  >  1:44450/1‑174 (MQ=255)                                                                                                                                                                                                      tATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAAc                                                                                   >  1:738658/1‑204 (MQ=255)                                                                                                                                                                                                      tATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTc                                     >  1:128961/1‑250 (MQ=255)                                                                                                                                                                                                       aTTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTc                                     >  1:723054/1‑249 (MQ=255)                                                                                                                                                                                                       aTTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCAGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTAtttt                                                                         >  1:82153/1‑213 (MQ=255)                                                                                                                                                                                                        ttCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTa                                  <  1:164599/251‑1 (MQ=255)                                                                                                                                                                                                        ttCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTa                                  <  1:42268/251‑1 (MQ=255)                                                                                                                                                                                                         tCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTat                                 <  1:10369/251‑1 (MQ=255)                                                                                                                                                                                                         tCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTat                                 <  1:668492/251‑1 (MQ=255)                                                                                                                                                                                                          cTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTAtttt                                                                         >  1:644618/1‑210 (MQ=255)                                                                                                                                                                                                           tATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTatga                               <  1:689190/251‑1 (MQ=255)                                                                                                                                                                                                           tATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTataa                               <  1:74041/251‑3 (MQ=255)                                                                                                                                                                                                            aTCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTa                                                                                                                           >  1:321158/1‑159 (MQ=255)                                                                                                                                                                                                            aTCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGa                                                       >  1:784712/1‑226 (MQ=255)                                                                                                                                                                                                            aTCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTatgat                              >  1:888109/1‑251 (MQ=255)                                                                                                                                                                                                            aTCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTCGCAAAACGACCAAGGGCGCACGCTTTCCTatgat                              >  1:319702/1‑251 (MQ=255)                                                                                                                                                                                                             tCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTatgatg                             <  1:838732/251‑1 (MQ=255)                                                                                                                                                                                                                  gcgAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAAtt                        >  1:645792/3‑251 (MQ=255)                                                                                                                                                                                                                     aaGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTatga                               <  1:843960/241‑1 (MQ=255)                                                                                                                                                                                                                     aaGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCAGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTcg                      >  1:437426/1‑249 (MQ=255)                                                                                                                                                                                                                         aaaaaaTGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGtt                 >  1:125851/1‑251 (MQ=255)                                                                                                                                                                                                                               tggtgATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGCTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTac           <  1:74814/247‑1 (MQ=255)                                                                                                                                                                                                                                   gATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTatga                               <  1:608583/227‑1 (MQ=255)                                                                                                                                                                                                                                   gATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTat                                 >  1:672766/1‑225 (MQ=255)                                                                                                                                                                                                                                        aaTCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGa                                                                    <  1:234638/185‑1 (MQ=255)                                                                                                                                                                                                                                        aaTCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCGCCGTTTATTACAACAAACga  >  1:797534/1‑251 (MQ=255)                                                                                                                                                                                                                                        aaTCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTAACTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTat                                 >  1:644556/1‑220 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      TCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTGGTCGATTATATTCCGTTGCATGAACGCCTAGGCACCATCGAAGATACTCCGGAATTATTCTTACGCCAACATAAGAATTATTTCCACTTATTCTATCCAAGTGAAGCAAAAAACGGCGATTTAATCACAGCAGCCTCTTTGCTAAAATCCTGCTTACGGATGAAGCCTGATCGTATTCTGCTTGCGGAATTACGCGGTGGGGAAACTTATGATTTTATCAATGTGGTTTCTTCCGGGCATAACGGGTCGATTACCTCTTGTCAT‑GCCGGTTCGGTTGCTGAAACCTGGGAACGGCTTATTTTAATGACGTTGCAAAACGACCAAGGGCGCACGCTTTCCTATGATGTAATTCACCGTTTATTACAACAAACGA  >  1/1452210‑1452691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑