Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,452,318	G→A	L185L (CTG→CTA)	unknown →	ATPase required for both assembly of type IV secretion complex and secretion of T‑DNA complex, VirB11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,452,318	0	G	A	100.0%	242.4 / NA	70	L185L (CTG→CTA)	unknown	ATPase required for both assembly of type IV secretion complex and secretion of T‑DNA complex, VirB11
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/29);  total (41/29)

CCATCAGTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTGGTCGATTATATTCCGTTGCATGAACGCCTAGGCACCATCGAAGATACTCCGGAATTATTCTTACGCCAACATAAGAATTATTTCCACTTATTCTATCCAAGTGAAGCAAAAAACGGCGATTTAATCACAGCAGCCTCTTTGCTAAAATCCTGCTTACGGATGAAGCCTGATCGTATTCTGCTTGCGGAATTACGCGGTGGGGAAACTTATGATTTTATCAATGTGGTTTCTTCCGGGCATAACGG  >  1/1452074‑1452563                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ccATCAGTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGAt                                                                                                                                                                                                                                                 >  1:529214/1‑251 (MQ=255)     cAGTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATtata                                                                                                                                                                                                                                             >  1:665791/1‑251 (MQ=255)       gTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATAtt                                                                                                                                                                                                                                           >  1:720757/1‑251 (MQ=255)       gTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATAtt                                                                                                                                                                                                                                           >  1:235783/1‑251 (MQ=255)        tATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATtata                                                                                                                                                                                                                                             <  1:472662/248‑1 (MQ=255)        tATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTc                                                                                                                                                                                                                                          <  1:148275/251‑1 (MQ=255)             cGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTg                                                                                                                                                                                                                                     <  1:508718/251‑1 (MQ=255)                     aaaCCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACg                                                                                                                                                                                                                             <  1:213533/251‑1 (MQ=255)                          gAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCtgg                                                                                                                                                                                                                        >  1:37453/1‑249 (MQ=255)                          gAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTATGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCtgg                                                                                                                                                                                                                        >  1:423158/1‑249 (MQ=255)                              aaGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCtggggac                                                                                                                                                                                                                    <  1:728520/251‑7 (MQ=255)                              aaGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCtggggac                                                                                                                                                                                                                    <  1:193704/251‑7 (MQ=255)                                gTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCa                                                                                                                                                                                                                  >  1:564395/1‑251 (MQ=255)                                   tttttttGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCAttg                                                                                                                                                                                                               <  1:118863/251‑3 (MQ=255)                                               ggATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTc                                                                                                                                                                                                                                          <  1:381927/212‑1 (MQ=255)                                               ggATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCtggggacc                                                                                                                                                                                                                   >  1:569426/1‑228 (MQ=255)                                               ggATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATAcccc                                                                                                                                                                                                     >  1:222634/1‑246 (MQ=255)                                               ggATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCgg                                                                                                                                                                                                   >  1:667406/1‑251 (MQ=255)                                               ggATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCgg                                                                                                                                                                                                   >  1:72941/1‑251 (MQ=255)                                               ggATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCgg                                                                                                                                                                                                   >  1:333253/1‑251 (MQ=255)                                                gATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTatat                                                                                                                                                                                                                                            >  1:5820/1‑209 (MQ=255)                                                  ttattaCATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTGTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACg                                                                                                                                                                                                                             <  1:441469/222‑1 (MQ=255)                                                      taCATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAattatt                                                                                                                                                                                            >  1:880128/1‑251 (MQ=255)                                                            aacaacGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACg                                                                                                                                                                                      <  1:697527/251‑1 (MQ=255)                                                                       ttttAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCtggggac                                                                                                                                                                                                                    <  1:713014/210‑7 (MQ=255)                                                                         ttAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGaac                                                                                                                                                                         <  1:697758/251‑2 (MQ=255)                                                                                   tATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACt                                                                                                                                                               <  1:82622/251‑1 (MQ=255)                                                                                         gACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACAt                                                                                                                                                                               >  1:92988/1‑229 (MQ=255)                                                                                          aCGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTc                                                                                                                                                          <  1:121596/249‑1 (MQ=255)                                                                                           cGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTAt                                                                                                                                                       >  1:700861/1‑251 (MQ=255)                                                                                            ggAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTc                                                                                                                                                                                                                                                    <  1:876584/157‑1 (MQ=255)                                                                                                     tttGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAg                                                                                                                                             >  1:63083/1‑251 (MQ=255)                                                                                                      ttGTCAGATGATGACTGGACACTACTGGAAATTCATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGc                                                                                                                                            <  1:620303/251‑1 (MQ=255)                                                                                                       tGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCtggggac                                                                                                                                                                                                                    >  1:241233/1‑172 (MQ=255)                                                                                                        gTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCaa                                                                                                                                          >  1:834854/1‑251 (MQ=255)                                                                                                            gatgatGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCAttg                                                                                                                                                                                                               <  1:796907/178‑3 (MQ=255)                                                                                                            gatgatGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGaac                                                                                                                                                                         >  1:843645/1‑215 (MQ=255)                                                                                                                 tgaCTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCg                                                                                                                                                                                                                                                   <  1:560036/137‑1 (MQ=255)                                                                                                                           ctactGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATc                                                                                                                                                      <  1:598776/220‑1 (MQ=255)                                                                                                                           ctactGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATca                                                                                                                        >  1:467232/1‑250 (MQ=255)                                                                                                                           ctactGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATca                                                                                                                        >  1:310402/1‑250 (MQ=255)                                                                                                                           ctactGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATca                                                                                                                        >  1:161532/1‑250 (MQ=255)                                                                                                                               tGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATc                                                                                                                                                      >  1:392256/1‑216 (MQ=255)                                                                                                                                ggAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAgcg                                                                                                                                                <  1:282654/221‑3 (MQ=255)                                                                                                                                        aTCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCtggggac                                                                                                                                                                                                                    <  1:567171/145‑7 (MQ=255)                                                                                                                                        aTCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAg                                                                                                                                                                            <  1:566925/185‑1 (MQ=255)                                                                                                                                         tCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATcacggc                                                                                                                    >  1:437769/1‑237 (MQ=255)                                                                                                                                         tCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTg                                                                                                          >  1:806088/1‑250 (MQ=255)                                                                                                                                             aaGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACACCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCtgaa                                                                                                     >  1:437037/1‑248 (MQ=255)                                                                                                                                                    aTTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATcttg                                                                                               >  1:420622/1‑247 (MQ=255)                                                                                                                                                         gCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCAc                                                                                                                                                                >  1:479340/1‑180 (MQ=255)                                                                                                                                                                tCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGt                                                                                                                                                                                                                                       <  1:253923/102‑1 (MQ=255)                                                                                                                                                                tCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGaaa                                                                                  >  1:830234/1‑250 (MQ=255)                                                                                                                                                                tCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGaaa                                                                                  >  1:637024/1‑250 (MQ=255)                                                                                                                                                                 ccTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGaaa                                                                                  >  1:153121/1‑249 (MQ=255)                                                                                                                                                                 ccTTAAACAAGCGGTCATTTGCGGGAAAAATATTGAGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGaaa                                                                                  >  1:614619/1‑249 (MQ=255)                                                                                                                                                                  cTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAATCCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGaaacc                                                                                <  1:682227/251‑4 (MQ=255)                                                                                                                                                                                cATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTAttttac                                                                  <  1:714019/251‑5 (MQ=255)                                                                                                                                                                                cATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTAttttac                                                                  <  1:809806/251‑5 (MQ=255)                                                                                                                                                                                              atatTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTAcga                                                    >  1:255552/1‑250 (MQ=255)                                                                                                                                                                                                   gtgATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTAttttactggcg                                                             >  1:166111/1‑228 (MQ=255)                                                                                                                                                                                                               gAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGa                                   >  1:608713/1‑251 (MQ=255)                                                                                                                                                                                                                          cGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAAtg                        <  1:323651/251‑1 (MQ=255)                                                                                                                                                                                                                           gggAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAAc                                         >  1:325609/1‑233 (MQ=255)                                                                                                                                                                                                                                     cTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAat                                                                                                                                                                                                >  1:323454/1‑72 (MQ=255)                                                                                                                                                                                                                                     cTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTttcttc              >  1:263081/1‑250 (MQ=255)                                                                                                                                                                                                                                         aaTGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGc         <  1:119083/251‑1 (MQ=255)                                                                                                                                                                                                                                                gCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGACTTTATCAATGTGGTTTCTTCCGGGCATAACgg  >  1:520970/1‑251 (MQ=255)                                                                                                                                                                                                                                                 cccTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCa                                                                                                                                                                 <  1:187583/91‑1 (MQ=255)                                                                                                                                                                                                                                                 cccTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACCGGATCGTATTTTACTGGCGGAATTACGCGGTGGGGAAACTTATGac                                  >  1:104685/1‑217 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      CCATCAGTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTGGTCGATTATATTCCGTTGCATGAACGCCTAGGCACCATCGAAGATACTCCGGAATTATTCTTACGCCAACATAAGAATTATTTCCACTTATTCTATCCAAGTGAAGCAAAAAACGGCGATTTAATCACAGCAGCCTCTTTGCTAAAATCCTGCTTACGGATGAAGCCTGATCGTATTCTGCTTGCGGAATTACGCGGTGGGGAAACTTATGATTTTATCAATGTGGTTTCTTCCGGGCATAACGG  >  1/1452074‑1452563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑