| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,446,394 | C→T | S180L (TCG→TTG) | unknown → | Tetracenomycin polyketide synthesis O‑methyltransferase TcmP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,446,394 | 0 | C | T | 100.0% | 267.0 / NA | 80 | S180L (TCG→TTG) | unknown | Tetracenomycin polyketide synthesis O‑methyltransferase TcmP |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (37/43); total (37/43) | |||||||||||
GCATTGCGCAAGCAACTGATGCCGCCGGCGGAAAATGAAATTTTGCTTGCCGCGTCGGCGTTTGACACGGATTGGATGGACGAATTACGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTCGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAGCCGCCATCATGTGGATTGTGCCCTTCGTGCGCAAATCTTATCGTTTCCTGTATTATCGGATAAGTTAAAATTCACCCTAAAAA > 1/1446147‑1446635 | gCATTGCGCAAGCAACTGATGCCGCCGGCGGAAAATGAAATTTTGCTTGCCGCGTCGGCGTTTGACACGGATTGGATGGACGAATTACGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCa > 1:682262/1‑251 (MQ=255) agcaaCTGATGCCGCCGGCGGAAAATGAAATTTTGCTTGCCGCGTCGGCGTTTGACACGGATTGGATGGACGAATTACGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCAt > 1:5809/1‑251 (MQ=255) ccgccgGCGGAAAATGAAATTTTGCTTGCCGCGTCGGCGTTTGACACGGATTGGATGGACGAATTACGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGtgatga < 1:320728/250‑1 (MQ=255) cggAAAATGAAATTTTGCTTGCCGCGTCGGCGTTTGACACGGATTGGAT‑GACGAATTACGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACAGATg < 1:535798/250‑1 (MQ=255) aaaTGAAATTTTGCTTGCCGCGTCGGCGTTTGACACGGATTGGATGGACGAATTACGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCggg > 1:779090/1‑251 (MQ=255) tGAAATTTTGCTTGCCGCGTCGGCGTTTGACACGGATTGGATGGACGAATTACGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGcac < 1:14797/251‑1 (MQ=255) aTTTTGCTTGCCGCGTCGGCGTTTGACACGGATTGGATGGACGAATTACGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGtt < 1:577824/251‑1 (MQ=255) ccGCGTCGGCGTTTGACACGGATTGGATGGACGAATTACGTTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGc < 1:160425/251‑1 (MQ=255) tttGACACGGATTGGATGGACGAATTACGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCgatgatga < 1:345646/251‑1 (MQ=255) gATTGGATGGACGAATTACGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATc < 1:133264/244‑1 (MQ=255) gATTGGATGGACGAATTACGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAAt > 1:832314/1‑251 (MQ=255) gATTGGATGGACGAATTACGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAAt > 1:362550/1‑251 (MQ=255) gATTGGATGGACGAATTACGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAAt > 1:831181/1‑251 (MQ=255) aTTGGATGGACGAATTACGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAAt < 1:556755/250‑1 (MQ=255) aTTGGATGGACGAATTACGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAAt < 1:378976/250‑1 (MQ=255) gatggaCGAATTACGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGaa < 1:215343/250‑1 (MQ=255) gaCGAATTACGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTg > 1:859823/1‑251 (MQ=255) cGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACat < 1:305571/189‑1 (MQ=255) ccACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGc > 1:198349/1‑241 (MQ=255) ccACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAAtct > 1:631693/1‑250 (MQ=255) ccACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAAtct > 1:205911/1‑250 (MQ=255) ggattattccgcgtttcgTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCa > 1:361927/18‑251 (MQ=255) aCCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACg > 1:694175/1‑172 (MQ=255) aCCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCa > 1:662756/1‑251 (MQ=255) cTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCAcc < 1:681580/251‑1 (MQ=255) gCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGAttt > 1:759890/1‑251 (MQ=255) ggCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCgatg < 1:208815/203‑1 (MQ=255) ggCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAAc < 1:495045/221‑1 (MQ=255) cacaGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGc < 1:5479/250‑1 (MQ=255) gTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAAc > 1:614156/1‑164 (MQ=255) gTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGt > 1:61575/1‑251 (MQ=255) tATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATg < 1:130949/251‑1 (MQ=255) tATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATg < 1:801808/251‑1 (MQ=255) aTTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGt < 1:315058/251‑1 (MQ=255) tttGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGtt < 1:801597/251‑1 (MQ=255) tttGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGtt < 1:499194/251‑1 (MQ=255) tttGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGtt < 1:102600/251‑1 (MQ=255) tttGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGtt < 1:817873/251‑1 (MQ=255) ttGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGttt < 1:672549/251‑1 (MQ=255) tATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGAtt > 1:402865/1‑251 (MQ=255) aTTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCga < 1:716055/182‑1 (MQ=255) ggCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCg > 1:622439/1‑250 (MQ=255) gTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTa < 1:463543/164‑1 (MQ=255) gTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTa > 1:8646/1‑164 (MQ=255) gTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGc > 1:712820/1‑248 (MQ=255) gATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGaaa > 1:818090/1‑250 (MQ=255) gATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGaaa > 1:410875/1‑250 (MQ=255) aTGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACg < 1:63745/251‑1 (MQ=255) aTGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCATTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTa < 1:408970/158‑1 (MQ=255) aTTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTa < 1:474587/154‑1 (MQ=255) aTTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAAc > 1:628207/1‑183 (MQ=255) aTTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTAt > 1:174395/1‑250 (MQ=255) aTTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTAt > 1:72867/1‑250 (MQ=255) tttCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGt < 1:784596/123‑1 (MQ=255) tCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGc < 1:394407/251‑1 (MQ=255) aTGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCttg > 1:658651/1‑251 (MQ=255) cgcAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGc < 1:221595/249‑1 (MQ=255) cAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGAtt < 1:161225/139‑1 (MQ=255) ggTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAgccg > 1:537477/1‑251 (MQ=255) tGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAgccgc > 1:645155/1‑250 (MQ=255) ttGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAGCCGCCATCATGTGGAtt > 1:409233/1‑251 (MQ=255) aTCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCgatgatgat < 1:595198/134‑1 (MQ=255) aTCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAGCCGCCATCATGTGGATTGTGccc < 1:343044/251‑1 (MQ=255) ccgtTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCt > 1:143124/1‑209 (MQ=255) ccgtTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAGCCGCCATCATGTGGATTGTGCCCTTCg < 1:396213/245‑1 (MQ=255) gtTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCAGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAGCCGCCATCATGTGGATTGTGCCCTTCGTGCGCaaa > 1:863929/1‑251 (MQ=255) ttttAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAGCCGCCATCATGTGGATTGTGCCCTTCGTGCGCAAAt > 1:252817/1‑251 (MQ=255) gtggCAAAATCGCCTTTGACATCGGCAGAAGTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGTGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCAAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAGCCGCCATCATGTGGATTGTGCCCTTCGTGCGCAAATCTTATCg < 1:434541/250‑1 (MQ=255) ggCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAGCCGCCATCATGTGGATTGTGCCCTTCGTGCGCAAATCTTATCGttt > 1:4892/1‑251 (MQ=255) gAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAGCCGCCATCATGTGGATTGTGccc > 1:224250/1‑225 (MQ=255) tCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACCCCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAGCCGCCATCATGTGGATTGTGCCCTTCGTGCGCAAATCTTATCGTTTCCTGtat < 1:518485/251‑1 (MQ=255) tttGACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAGCCGCCATCATGTGGATTGTGCCCTTCGTGCGCAAATCTTATCGTTTCCTGTATTATCg < 1:613684/251‑1 (MQ=255) gACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAACCCCCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAGCCGCCATCATGTGGATTGTGCCCTTCGTGCGCAAATCTTATCGTTTCCTGtat < 1:891484/243‑1 (MQ=255) gACATCGGCAGCACTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAGCCGCCATCATGTGGATTGTGCCCTTCGTGCGCAAATCTTATCGTTTCCTGTATTATCGGAt > 1:188913/1‑251 (MQ=255) gcaCTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGtt > 1:693150/1‑142 (MQ=255) gcaCTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAGCCGCCATCATGTGGATTGTGCCCTTCGTGCGCAAATCTTATCGTTTCCTGTATTATCGGATAAGTTAAAAt > 1:887963/1‑251 (MQ=255) aCTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAGCCGCCATCATGTGGATTGTGCCCTTCGTGCGCAAATCTTATCg < 1:23346/221‑1 (MQ=255) aCTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAGCCGCCATCATGTGGATTGTGCCCTTCGTGCGCAAATCTTATCGTTTCCTGTATTATCGGATAAGTTAAAATTc < 1:461168/251‑1 (MQ=255) cTTGGATGAGTCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGAt < 1:211078/143‑1 (MQ=255) tCGCAATTTGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAGCCGCCATCATGTGGATTGTGCCCTTCGTGCGCAAATCTTATCGTTTCCTGTATTATCGGATAAGTTAAAATTCACCCTaaaaa < 1:387101/250‑1 (MQ=255) | GCATTGCGCAAGCAACTGATGCCGCCGGCGGAAAATGAAATTTTGCTTGCCGCGTCGGCGTTTGACACGGATTGGATGGACGAATTACGCTCCACCTATCCGCAGGCACAGTTTTTATTTGTTATTGAAGGCGTATTGATGTATTTCAATGAAGCGCAGGTGAAAAAGCTGTTTGAAGATCTTGCCGTCCGTTTTAGTGGTGGCGAAATCGCCTTTGACATCGGCAGCACTTGGATGAGTCGCAATTCGCAAAAACACCATGACGTGATGAAACATATGCGGGCACAGTTTGATTACGGCTGCGATGATGATCATGAAATGGAACGTTGGGCGGATAATCTCCACCTGATTTCGGCGAAGTATATGTTTGATTTTCCCGCCTGGAAACGTATCGGCTTGTTGCAAGCCGCCATCATGTGGATTGTGCCCTTCGTGCGCAAATCTTATCGTTTCCTGTATTATCGGATAAGTTAAAATTCACCCTAAAAA > 1/1446147‑1446635 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |