Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,445,545	T→C	intergenic (+78/‑311)	unknown → / → unknown	Uncharacterized protein YjgR/Tetracenomycin polyketide synthesis O‑methyltransferase TcmP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,445,545	0	T	C	100.0%	214.7 / NA	63	intergenic (+78/‑311)	unknown/unknown	Uncharacterized protein YjgR/Tetracenomycin polyketide synthesis O‑methyltransferase TcmP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/25);  total (38/25)

CATGGGCAGTCTCAGCCGCATGATTTTCGGCACCAAAAAACGCGGCGAACAACTCTCGGTTACCGAACAAGTCGTCAGCAGTGTGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCATAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAATCATCCTGTTCTCAAGAAGACAACATTCGTCTTTTCCCGACGGCAGCTTGCGTCGTACTGTCCGCCACACATGACTCTCTACGCGGTTTAAC  >  1/1445302‑1445780                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            catGGGCAGTCTCAGCCGCATGATTTTCGGCACCAAAAAACGCGGCGAACAACTCTCGGTTACCGAACAAGTCGTCAGCAGTGTGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAAt                                                                                                                                                                                                                                      >  1:339792/1‑251 (MQ=255)    gggCAGTCTCAGCCGCATGATTTTCGGCACCAAAAAACGCGGCGAACAACTCTCGGTTACCGAACAAGTCGTCAGCAGTGTGGC‑‑AATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGt                                                                                                                                                                                                                                 >  1:341559/1‑251 (MQ=255)         gTCTCAGCCGCATGATTTTCGGCACCAAAAAACGCGGCGAACAACTCTCGGTTACCGAACAAGTCGTCAGCAGTGTGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGtttt                                                                                                                                                                                                                              >  1:320628/1‑251 (MQ=255)         gTCTCAGCCGCATGATTTTCGGCACCAAAAAACGCGGCGAACAACTCTCGGTTACCGAACAAGTCGTCAGCAGTGTGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGtttt                                                                                                                                                                                                                              >  1:728087/1‑251 (MQ=255)          tctcAGCCGCATGATTTTCGGCACCAAAAAACGCGGCGAACAACTCTCGGTTACCGAACAAGTCGTCAGCAGTGTGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGttttt                                                                                                                                                                                                                             >  1:647422/1‑251 (MQ=255)                       aTTTTCGGCACCAAAAAACGCGGCGAACAACTCTCGGTTACCGAACAAGTCGTCAGCAGTGTGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTa                                                                                                                                                                                                                >  1:690962/1‑251 (MQ=255)                               cACCAAAAAACGCGGCGAACAACTCTCGGTTACCGAACAAGTCGTCAGCAGTGTGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAg                                                                                                                                                                                                         <  1:289140/250‑1 (MQ=255)                                 ccAAAAAACGCGGCGAACAACTCTCGGTTACCGAACAAGTCGTCAGCAGTGTGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAggg                                                                                                                                                                                                       >  1:809753/1‑250 (MQ=255)                                        aCGCGGCGAACAACTCTCGGTTACCGAACAAGTCGTCAGCAGTGTGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAg                                                                                                                                                                                               <  1:450850/251‑1 (MQ=255)                                                    acTCTCGGTTACCGAACAAGTCGTCAGCAGTGTGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACaaaaaa                                                                                                                                                                                                                                       >  1:856512/1‑199 (MQ=255)                                                    acTCTCGGTTACCGAACAAGTCGTCAGCAGTGTGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAg                                                                                                                                                                                               <  1:818534/239‑1 (MQ=255)                                                    acTCTCGGTTACCGAACAAGTCGTCAGCAGTGTGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAAt                                                                                                                                                                                   <  1:160936/251‑1 (MQ=255)                                                       ctcGGTTACCGAACAAGTCGTCAGCAGTGTGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACaaaa                                                                                                                                                                                                                                         <  1:82552/194‑1 (MQ=255)                                                         cGGTTACCGAACAAGTCGTCAGCAGTGTGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATa                                                                                                                                                                                      >  1:416817/1‑243 (MQ=255)                                                                          gtcAGCAGTGTGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAatat                                                                                                                                                             >  1:386130/1‑251 (MQ=255)                                                                                 gtgtGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGcc                                                                                                                                                                                                            >  1:202352/1‑197 (MQ=255)                                                                                 gtgtGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAg                                                                                                                                                                                               >  1:826352/1‑210 (MQ=255)                                                                                 gtgtGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCa                                                                                                                                                      >  1:679447/1‑251 (MQ=255)                                                                                   gtgGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCaaa                                                                                                                                                    >  1:68981/1‑251 (MQ=255)                                                                                     ggCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACg                                                                                                                                                  >  1:492859/1‑251 (MQ=255)                                                                                                       cAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAAt                                                                                                                                 >  1:850915/1‑250 (MQ=255)                                                                                                       cAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATc                                                                                                                                <  1:666052/251‑1 (MQ=255)                                                                                                        aaTATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATcc                                                                                                                               <  1:200635/251‑1 (MQ=255)                                                                                                           atTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAg                                                                                                                            >  1:627007/1‑251 (MQ=255)                                                                                                            ttCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATc                                                                                                                                <  1:890399/246‑1 (MQ=255)                                                                                                             tCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGcc                                                                                                                          <  1:91901/251‑1 (MQ=255)                                                                                                                              aacaaaTTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTttaatta                                                                                                                  <  1:223716/242‑1 (MQ=255)                                                                                                                              aacaaaTTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAAtt                                                                                                         >  1:578840/1‑251 (MQ=255)                                                                                                                                    ttATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATc                                                                                                   <  1:870828/251‑1 (MQ=255)                                                                                                                                          gcgGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATc                                                                                                                                                                                  >  1:245079/1‑166 (MQ=255)                                                                                                                                          gcgGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAat                                                                                             >  1:442065/1‑251 (MQ=255)                                                                                                                                           cgGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAgg                                                                                                                                                                                              >  1:24338/1‑153 (MQ=255)                                                                                                                                             gTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCa                                                                                                                                                                                                           >  1:532509/1‑138 (MQ=255)                                                                                                                                             gTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAg                                                                                                                                                                                               >  1:268560/1‑150 (MQ=255)                                                                                                                                             gTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTg                                                                                                                                                                                          >  1:15503/1‑155 (MQ=255)                                                                                                                                             gTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGa                                                                                                                                                                        <  1:522992/173‑1 (MQ=255)                                                                                                                                             gTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAAc                                                                                                                                                   >  1:84338/1‑194 (MQ=255)                                                                                                                                             gTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATc                                                                                                                                <  1:666628/213‑1 (MQ=255)                                                                                                                                               aTTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTc                                                                                                                                                                             <  1:64781/166‑1 (MQ=255)                                                                                                                                               aTTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAATCATcc                                                                                        <  1:144000/251‑1 (MQ=255)                                                                                                                                                         cgTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAATCATCCTGTTCTCaag                                                                              <  1:468149/251‑1 (MQ=255)                                                                                                                                                           tttAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGcc                                                                                                                          <  1:630272/205‑1 (MQ=255)                                                                                                                                                                   gTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAg                                                                                                                                                                                               <  1:820196/128‑1 (MQ=255)                                                                                                                                                                   gTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGaa                                                                                                              <  1:630688/209‑1 (MQ=255)                                                                                                                                                                             cccGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAATCATCCTGTTCTCAAGAAGACAACATTCGTCtttt                                                           >  1:279036/1‑250 (MQ=255)                                                                                                                                                                                               cTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAgg                                                                                                                                                                                                        >  1:305626/1‑91 (MQ=255)                                                                                                                                                                                                 tttCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATcc                                                                                                                               >  1:208548/1‑162 (MQ=255)                                                                                                                                                                                                 tttCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATc                                                                                                                                >  1:856218/1‑161 (MQ=255)                                                                                                                                                                                                 tttCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAATCATCCTGTtc                                                                                   <  1:56794/206‑1 (MQ=255)                                                                                                                                                                                                   tCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAATCATCCTGTTCTCAAGAAGACAACATTCGTCTTTTCCCGACGGCAGCTTGCGTCGTAc                                    <  1:598974/251‑1 (MQ=255)                                                                                                                                                                                                   tCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAATCATCCTGTTCTCAAGAAGACAACATTCGTCTTTTCCCGACGGCAGCTTGCGTCGTAc                                    <  1:370921/251‑1 (MQ=255)                                                                                                                                                                                                      cTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATaa                                                                                              >  1:536494/1‑190 (MQ=255)                                                                                                                                                                                                      cTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAATCATCCTGTTCTCAAGAAGACAACATTCGTCTTTTCCCGACGGCAGCTTGCGTCGTa                                     >  1:885230/1‑247 (MQ=255)                                                                                                                                                                                                      cTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAATCATCCTGTTCTCAAGAAGACAACATTCGTCTTTTCCCGACGGCAGCTTGCGTCGTACTg                                  <  1:135291/250‑1 (MQ=255)                                                                                                                                                                                                                  cAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAATCATCCTGTTCTCAAGAAGACAACATTCGTCTTTTCCCGACGGCAGCTTGCGTCGTACTGTCCGCCACACATg                     >  1:860953/1‑251 (MQ=255)                                                                                                                                                                                                                  cAACATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAATCATCCTGTTCTCAAGAAGACAACATTCGTCTTTTCCCGACGGCAGCTTGCGTCGTACTGTCCGCCACACATg                     >  1:638618/1‑251 (MQ=255)                                                                                                                                                                                                                   aaCATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAATCATCCTGTTCTCAAGAAGACAACATTCGTCTTTTCCCGACGGCAGCTTGCGTCGTACTGTCCGCCACACATGa                    >  1:845362/1‑251 (MQ=255)                                                                                                                                                                                                                     cATAAGCATCTCATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAATCATCCTGTTCTCAAGAAGACAACATTCGTCTTTTCCCGACGGCAGCTTGCGTCGTACTGTCCGCCACACATGa                    >  1:682045/1‑249 (MQ=255)                                                                                                                                                                                                                             tctcATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTttaatta                                                                                                                  >  1:256963/1‑147 (MQ=255)                                                                                                                                                                                                                             tctcATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAATCATCCTGTTCTCAAGAAGacaa                                                                       <  1:3234/190‑1 (MQ=255)                                                                                                                                                                                                                              ctcATACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAATCATCCTGTTCTCAAGAAGACAACATTCGTCTTTTCCCGACGGCAGCTTGCGTCGTACTGTCCGCCACACATGACTCTCTAcgcg         >  1:342119/1‑251 (MQ=255)                                                                                                                                                                                                                                  tACCCAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAATCATCCTGTTCTCAAGAAGACAACATTCGTCTTTTCCCGACGGCAGCTTGCGTCGTACTGTCCGCCACACATGACTCTCTACGCGGttt     <  1:199115/251‑1 (MQ=255)                                                                                                                                                                                                                                      cAAAAGTGCGGTCACAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAATCATCCTGTTCTCAAGAAGACAACATTCGTCTTTTCCCGACGGCAGCTTGCGTCGTACTGTCCGCCACACATGACTCTCTACGCGGTTTAAc  >  1:860294/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            CATGGGCAGTCTCAGCCGCATGATTTTCGGCACCAAAAAACGCGGCGAACAACTCTCGGTTACCGAACAAGTCGTCAGCAGTGTGGCAAAATCCGTCGGTCGCAATATTCGCAACCAAGTGACAAAACAAATTATGCGCGGTATTCTGGGCGCGTTTAAGAAGTAAAATTCACCCGTAAAAATGACCGCACTTTTCGCTATTGGGCTTGCAACATAAGCATCTCATACCCAAAAGTGCGGTCATAAAAAATTCCGTTTTTTGAACGTTGGTTATGCCAAGGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTCCAAACGTTTTTCACCCCCTTAATCCCAGCCTTTAATTAAGAATAATTCTCATCTATAATCATCCTGTTCTCAAGAAGACAACATTCGTCTTTTCCCGACGGCAGCTTGCGTCGTACTGTCCGCCACACATGACTCTCTACGCGGTTTAAC  >  1/1445302‑1445780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑