Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,307,428 T→C H235R (CAT→CGT)  unknown ← Twin‑arginine translocation protein TatC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,307,4280TC100.0% 420.7 / NA 123H235R (CAT→CGT) unknownTwin‑arginine translocation protein TatC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (67/56);  total (67/56)

Only 100 of 123 total aligned reads displayed.

CATGGAAGGCACGGCTTCGCGGTGATTTTCGCCTTCAATAATAAACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAATGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCGGCACTTCAAAACAAACGCCGAAAGCGAGGAACAGGGTGAGCACGAAATCCAGATAAC  >  1/1307181‑1307671
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   
cATGGAAGGCACGGCTTCGCGGTGATTTTCGCCTTCAATAATAAACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACggg                                                                                                                                                                                                                                                  <  1:192886/251‑1 (MQ=255)
  tGGAAGGCACGGCTTCGCGGTGATTTTCGCCTTCAATAATAAACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGgcg                                                                                                                                                                                                                                                >  1:41188/1‑251 (MQ=255)
        gCACGGCTTCGCGGTGATTTTCGCCTTCAATAATAAACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAg                                                                                                                                                                                                                                           <  1:737459/250‑1 (MQ=255)
          aCGGCTTCGCGGTGATTTTCGCCTTCAATAATAAACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGcac                                                                                                                                                                                                                                        <  1:875505/251‑1 (MQ=255)
              cTTCGCGGTGATTTTCGCCTTCAATAATAAACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACAcc                                                                                                                                                                                                                                     <  1:831023/250‑1 (MQ=255)
                        aTTTTCGCCTTCAATAATAAACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATa                                                                                                                                                                                                                          >  1:404698/1‑251 (MQ=255)
                              gCCTTCAATAATAAACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAg                                                                                                                                                                                                                     >  1:738540/1‑250 (MQ=255)
                                cTTCAATAATAAACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCa                                                                                                                                                                                                                                   <  1:647144/234‑1 (MQ=255)
                                   cAATAATAAACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACAt                                                                                                                                                                                                                >  1:541773/1‑250 (MQ=255)
                                    aataatAAACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGca                                                                                                                                                                                                                                         <  1:110303/224‑1 (MQ=255)
                                     ataataAACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCa                                                                                                                                                                                                                                   >  1:244502/1‑229 (MQ=255)
                                     ataataAACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCa                                                                                                                                                                                                                                   <  1:564118/229‑1 (MQ=255)
                                     ataataAACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATATGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACa                                                                                                                                                                                                                       <  1:822444/241‑1 (MQ=255)
                                       aataaACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAg                                                                                                                                                                                                                                           <  1:766783/219‑1 (MQ=255)
                                       aataaACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATc                                                                                                                                                                                                               <  1:829993/247‑1 (MQ=255)
                                       aataaACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCgggg                                                                                                                                                                                                           >  1:811621/1‑251 (MQ=255)
                                        ataaACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGc                                                                                                                                                                                                          <  1:794030/251‑1 (MQ=255)
                                        ataaACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGc                                                                                                                                                                                                          <  1:77491/251‑1 (MQ=255)
                                        ataaACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGc                                                                                                                                                                                                          <  1:893476/251‑1 (MQ=255)
                                               cGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCgggg                                                                                                                                                                                                           >  1:844319/1‑243 (MQ=255)
                                                 ggATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTaa                                                                                                                                                                                                 >  1:461570/1‑251 (MQ=255)
                                                 ggATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTaa                                                                                                                                                                                                 >  1:644984/1‑251 (MQ=255)
                                                 ggATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTaa                                                                                                                                                                                                 >  1:753094/1‑251 (MQ=255)
                                                 ggATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTaa                                                                                                                                                                                                 >  1:345509/1‑251 (MQ=255)
                                                 ggATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTaa                                                                                                                                                                                                 >  1:137757/1‑251 (MQ=255)
                                                 ggATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTaa                                                                                                                                                                                                 >  1:261114/1‑251 (MQ=255)
                                                       aTCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCCTAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTtg                                                                                                                                                                                           >  1:217055/1‑251 (MQ=255)
                                                       aTCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGc                                                                                                                                                                                                                                          >  1:82586/1‑204 (MQ=255)
                                                           gATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCCTATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGGTTGTGaa                                                                                                                                                                                       >  1:45022/1‑251 (MQ=255)
                                                              cGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACAccc                                                                                                                                                                                                                                    <  1:824640/203‑1 (MQ=255)
                                                              cGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGcac                                                                                                                                                                                                                  <  1:880735/221‑1 (MQ=255)
                                                              cGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGc                                                                                                                                                                                                          >  1:691818/1‑229 (MQ=255)
                                                               gTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAAt                                                                                                                                                                                                                           >  1:859128/1‑211 (MQ=255)
                                                                tCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATa                                                                                                                                                                                   <  1:377765/250‑1 (MQ=255)
                                                                tCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATa                                                                                                                                                                                   >  1:260444/1‑250 (MQ=255)
                                                                    cGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTc                                                                                                                                                                               >  1:16565/1‑250 (MQ=255)
                                                                          gTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAgg                                                                                                                                                                                               <  1:675895/228‑1 (MQ=255)
                                                                          gTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGa                                                                                                                                                                        >  1:48493/1‑251 (MQ=255)
                                                                              gtgtTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGgcg                                                                                                                                                                                                                                                <  1:80043/175‑1 (MQ=255)
                                                                              gtgtTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCa                                                                                                                                                                    >  1:87219/1‑251 (MQ=255)
                                                                              gtgtTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCa                                                                                                                                                                    >  1:787311/1‑251 (MQ=255)
                                                                                gtTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGa                                                                                                                                                                  >  1:516423/1‑251 (MQ=255)
                                                                                       cATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATAc                                                                                                                                                           <  1:370140/251‑1 (MQ=255)
                                                                                          cAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGa                                                                                                                                                        <  1:590206/251‑1 (MQ=255)
                                                                                            aGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGCGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATAc                                                                                                                                                           >  1:588639/1‑246 (MQ=255)
                                                                                                gcgACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCTTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATaaag                                                                                                                                                   <  1:439970/250‑1 (MQ=255)
                                                                                                    cTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGc                                                                                                                                                               >  1:714476/1‑234 (MQ=255)
                                                                                                              gTTTACGCATGCGGCGTAAACGGTGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGt                                                                                                                                                                                              <  1:445205/193‑1 (MQ=255)
                                                                                                              gTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATaaag                                                                                                                                                   >  1:530674/1‑236 (MQ=255)
                                                                                                              gTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGata                                                                                                                                    >  1:528414/1‑251 (MQ=255)
                                                                                                              gTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGata                                                                                                                                    >  1:55580/1‑251 (MQ=255)
                                                                                                              gTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGata                                                                                                                                    >  1:479707/1‑251 (MQ=255)
                                                                                                                  aCGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAatat                                                                                                                                <  1:539308/251‑1 (MQ=255)
                                                                                                                  aCGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAatat                                                                                                                                <  1:662360/251‑1 (MQ=255)
                                                                                                                          ggcgTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGt                                                                                                                        >  1:796819/1‑251 (MQ=255)
                                                                                                                           gcgTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAgg                                                                                                                                                                                               >  1:669424/1‑179 (MQ=255)
                                                                                                                           gcgTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGtt                                                                                                                       >  1:75991/1‑251 (MQ=255)
                                                                                                                                aaCGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGAt                                                                                                                  <  1:62868/251‑1 (MQ=255)
                                                                                                                                 aCGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATc                                                                                                                 <  1:830982/251‑1 (MQ=255)
                                                                                                                                         tGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATc                                                                                                          <  1:682077/250‑1 (MQ=255)
                                                                                                                                                    gTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAAtata                                                                                                                               >  1:655132/1‑218 (MQ=255)
                                                                                                                                                               ttgCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTcc                                                                                   <  1:50569/251‑1 (MQ=255)
                                                                                                                                                                   gTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGtt                                                                                                                                                                                             <  1:302018/141‑1 (MQ=255)
                                                                                                                                                                   gTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAAt                                                                                                           >  1:577209/1‑223 (MQ=255)
                                                                                                                                                                   gTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATc                                                                                                          >  1:179652/1‑224 (MQ=255)
                                                                                                                                                                    tCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGcac                                                                              <  1:813235/251‑1 (MQ=255)
                                                                                                                                                                    tCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGACCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGcac                                                                              <  1:605503/251‑1 (MQ=255)
                                                                                                                                                                      atatTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAg                                                                            >  1:164620/1‑251 (MQ=255)
                                                                                                                                                                        atTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCAc                                                                                                                                                                                                                                  <  1:794630/99‑1 (MQ=255)
                                                                                                                                                                        atTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTa                                                                          >  1:83735/1‑251 (MQ=255)
                                                                                                                                                                        atTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGACAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTa                                                                          >  1:695643/1‑251 (MQ=255)
                                                                                                                                                                          tctctcCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTca                                                                                            <  1:302235/231‑1 (MQ=255)
                                                                                                                                                                          tctctcCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTaaa                                                                        >  1:12127/1‑251 (MQ=255)
                                                                                                                                                                           ctctcCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGCTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTaaaa                                                                       >  1:409792/1‑251 (MQ=255)
                                                                                                                                                                            tctcCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGTATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAaat                                                                      <  1:427262/251‑1 (MQ=255)
                                                                                                                                                                             ctcCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAaata                                                                     >  1:205606/1‑251 (MQ=255)
                                                                                                                                                                               ccTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTca                                                                                            >  1:457729/1‑226 (MQ=255)
                                                                                                                                                                               ccTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAaataat                                                                   <  1:684478/251‑1 (MQ=255)
                                                                                                                                                                                    aTCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAaataa                                                                    >  1:413284/1‑245 (MQ=255)
                                                                                                                                                                                     tCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAaataat                                                                   >  1:523245/1‑245 (MQ=255)
                                                                                                                                                                                        gATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTc                                                                                             >  1:707393/1‑216 (MQ=255)
                                                                                                                                                                                        gATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCgg                                                          >  1:885613/1‑251 (MQ=255)
                                                                                                                                                                                              tGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCGGCACTTc                                                    <  1:902123/251‑1 (MQ=255)
                                                                                                                                                                                               gCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACAcc                                                                                        <  1:347496/214‑1 (MQ=255)
                                                                                                                                                                                               gCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAaataat                                                                   >  1:130370/1‑235 (MQ=255)
                                                                                                                                                                                                             cTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCGGCACTTCAAAACAAACGCCGaa                                     >  1:193646/1‑251 (MQ=255)
                                                                                                                                                                                                               ttCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCGGCACTTCAAAACAAACGCCGAAAg                                   <  1:648707/251‑1 (MQ=255)
                                                                                                                                                                                                                           ctcGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCGGCACTTCAAAACAAACGCCGAAAGCGAGGAACAggg                       <  1:805927/251‑1 (MQ=255)
                                                                                                                                                                                                                            tcGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCGGCACTTCAAAACAAACGCCGAAAGCGAGGAACAGGGt                      <  1:187566/251‑1 (MQ=255)
                                                                                                                                                                                                                             cGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCGGCACTTCAAAACAAACGCCGAAAGCGAGGAACAGGGTg                     >  1:710547/1‑251 (MQ=255)
                                                                                                                                                                                                                             cGTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCGGCACTTCAAAACAAACGCCGAAAGCGAGGAACAGGGTg                     <  1:197251/251‑1 (MQ=255)
                                                                                                                                                                                                                              gTCATCTTCGCGTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCGGCACTTCAAAACAAACGCCGAAAGCGAGGAACAGGGTGa                    >  1:369594/1‑251 (MQ=255)
                                                                                                                                                                                                                                      cgcgTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAGATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCGGCACTTCAAAACAAACGCCGAAAGCGAGGAACAGGGTGAGCACGaaa            >  1:672727/1‑251 (MQ=255)
                                                                                                                                                                                                                                      cgcgTGGACGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCGGCACTTCAAAACAAACGCCGAAAGCGAGGAACAGGGTGAGCACGaaa            >  1:4323/1‑251 (MQ=255)
                                                                                                                                                                                                                                             aCGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCGGCACTTCAAAACAAACGCCGAAAGCGAGGAACAGGGTGAGCACGAAATCCAGa      <  1:138278/250‑1 (MQ=255)
                                                                                                                                                                                                                                              cGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCGGCACTTCAAAACAAACGCCGAAAGCGAGGAACAGGGTGAGCACGAAATCCAGATa    >  1:855244/1‑251 (MQ=255)
                                                                                                                                                                                                                                              cGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCGGCACTTCAAAACAAACGCCGAAAGCGAGGAACAGGGTGAGCACGAAATCCAGATa    >  1:712824/1‑251 (MQ=255)
                                                                                                                                                                                                                                              cGGTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCGGCACTTCAAAACAAAAGCCGAAAGCGAGGAACAGGGTGAGCACGAAATCCAGATa    >  1:333343/1‑251 (MQ=255)
                                                                                                                                                                                                                                                gTAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCGGCACTTCAAAACAAACGCCGAAAGCGAGGAACAGGGTGAGCACGAAATCCAGATaa   >  1:163751/1‑250 (MQ=255)
                                                                                                                                                                                                                                                 tAGAAACGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCGGCACTTCAAAACAAACGCCGAAAGCGAGGAACAGGGTGAGCACGAAATCCAGATAAc  <  1:382905/250‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   
CATGGAAGGCACGGCTTCGCGGTGATTTTCGCCTTCAATAATAAACACGGGATAAATCAGATCGTCAACGGTCAGTTTGTGTTCCGCCATCAAGCGGCGACTAAAGTCCTGTTTACGCATGCGGCGTAAACGGCGGGTGGGAAAACCGGTGAAAATTTGTTGCGTCATATTCTCTCCTGAATCTGATTATTGCTTTGCGCTGTCGCTTTCATTTTGCGTCTCGTCATCTTCGCGTGGACGGTAGAAATGGGCGCATAGCACACCCACTTCAAATAACAAGCACATCGGGGCAGCGAGTAAGGTTTGTGAAAATACGTCCGGCGGAGTCAGAAGCATACCGATAAAGAAAGCGGCTACGATAATATACGGACGTTTGGATCTTAAATCTTCCGGTGTAGTCACACCCGTCCAGCACAGTAAAATAATCGCCACCGGCACTTCAAAACAAACGCCGAAAGCGAGGAACAGGGTGAGCACGAAATCCAGATAAC  >  1/1307181‑1307671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑