BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,256,698	T→C	D381D (GAT→GAC)	unknown →	Type IV pilus biogenesis protein PilQ; Competence protein E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,256,698	0	T	C	100.0%	374.8 / NA	110	D381D (GAT→GAC)	unknown	Type IV pilus biogenesis protein PilQ; Competence protein E
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (51/59); total (51/59)

Only 100 of 110 total aligned reads displayed.

GCATCAAACAGGGGACGGAATTGCCCTATATTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGATGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAGCGAATCGGGGTAAATGAGCAAATCATGGCAAAAGGGGAGAAT  >  1/1256457‑1256941

gCATCAAACAGGGGACGGAATTGCCCTATATTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAAc                                                                                                                                                                                                                                             >  1:273967/1‑250 (MQ=255)

 cATCAAACAGGGGACGGAATTGCCCTATATTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAAcc                                                                                                                                                                                                                                            <  1:587149/250‑1 (MQ=255)

  aTCAAACAGGGGACGGAATTGCCCTATATTCTGGTGAACG‑AAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCAtt                                                                                                                                                                                                                                         >  1:639141/1‑251 (MQ=255)

    cAAACAGGGGACGGAATTGCCCTATATTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATtg                                                                                                                                                                                                                                        >  1:150760/1‑251 (MQ=255)

           gggACGGAATTGCCCTATATTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTggg                                                                                                                                                                                                                                 >  1:326785/1‑251 (MQ=255)

             gACGGAATTGCCCTATATTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGc                                                                                                                                                                                                                                <  1:503672/250‑1 (MQ=255)

              aCGGAATTGCCCTATATTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCgg                                                                                                                                                                                                                              <  1:687792/251‑1 (MQ=255)

                 gAATTGCCCTATATTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGACGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGgtgt                                                                                                                                                                                                                           >  1:444049/1‑251 (MQ=255)

                  aaTTGCCCTATATTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGgtgt                                                                                                                                                                                                                           <  1:595162/250‑1 (MQ=255)

                     tGCCCTATATTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCTGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTAttt                                                                                                                                                                                                                       <  1:437341/251‑1 (MQ=255)

                      gCCCTATATTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTa                                                                                                                                                                                                                      >  1:108631/1‑251 (MQ=255)

                      gCCCTATATTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTa                                                                                                                                                                                                                      >  1:518852/1‑251 (MQ=255)

                      gCCCTATATTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGACAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGc                                                                                                                                                                                                                                >  1:391407/1‑241 (MQ=255)

                       cccTATATTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAg                                                                                                                                                                                                                     <  1:332326/251‑1 (MQ=255)

                       cccTATATTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAg                                                                                                                                                                                                                     <  1:771048/251‑1 (MQ=255)

                        ccTATATTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATtg                                                                                                                                                                                                                                        <  1:527027/231‑1 (MQ=255)

                        ccTATATTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGc                                                                                                                                                                                                                    <  1:42202/251‑1 (MQ=255)

                           atatTCTGGTGAACGAAAAAAGCGGTTCGCAACGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGAt                                                                                                                                                                                                                 >  1:265925/1‑251 (MQ=255)

                           atatTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGAt                                                                                                                                                                                                                 >  1:595585/1‑251 (MQ=255)

                           atatTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGAt                                                                                                                                                                                                                 >  1:740288/1‑251 (MQ=255)

                             atTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATa                                                                                                                                                                                                                >  1:455375/1‑250 (MQ=255)

                             atTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATAc                                                                                                                                                                                                               <  1:365421/251‑1 (MQ=255)

                               tCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCa                                                                                                                                                                                                             <  1:541280/251‑1 (MQ=255)

                                    tGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCAcc                                                                                                                                                                                                        <  1:82186/251‑1 (MQ=255)

                                      aaCGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCa                                                                                                                                                                                                       <  1:880662/250‑1 (MQ=255)

                                         gAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAgg                                                                                                                                                                                                   >  1:379188/1‑251 (MQ=255)

                                                    ttCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAGCAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAg                                                                                                                                                                                        <  1:395553/251‑1 (MQ=255)

                                                            gCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAg                                                                                                                                                                                        >  1:762371/1‑243 (MQ=255)

                                                             cGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGc                                                                                                                                                                               <  1:642560/251‑1 (MQ=255)

                                                                     ttCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAAc                                                                                                                                                                                                                                             >  1:360028/1‑181 (MQ=255)

                                                                     ttCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGAc                                                                                                                                                                       <  1:107248/251‑1 (MQ=255)

                                                                         tGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTc                                                                                                                                                                   <  1:714217/251‑1 (MQ=255)

                                                                         tGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTc                                                                                                                                                                   <  1:721224/251‑1 (MQ=255)

                                                                             gCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGc                                                                                                                                                                                                  <  1:829241/216‑1 (MQ=255)

                                                                                gTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGc                                                                                                                                                                                                                                <  1:450356/183‑1 (MQ=255)

                                                                                gTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAg                                                                                                                                                                                                                     <  1:675027/194‑1 (MQ=255)

                                                                                gTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAg                                                                                                                                                                                                                     >  1:77002/1‑194 (MQ=255)

                                                                                gTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAg                                                                                                                                                                                        >  1:50154/1‑223 (MQ=255)

                                                                                gTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTa                                                                                                                                                             <  1:558684/250‑1 (MQ=255)

                                                                                gTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTAt                                                                                                                                                            >  1:679749/1‑251 (MQ=255)

                                                                                gTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTAt                                                                                                                                                            >  1:306487/1‑251 (MQ=255)

                                                                                  aCTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTa                                                                                                                                                          <  1:695025/251‑1 (MQ=255)

                                                                                     aGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAg                                                                                                                                                                                        <  1:552194/218‑1 (MQ=255)

                                                                                       gTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACAt                                                                                                                                                     >  1:594341/1‑251 (MQ=255)

                                                                                        tttGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACAt                                                                                                                                                     <  1:397875/250‑1 (MQ=255)

                                                                                            gAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTAt                                                                                                                                                 <  1:700941/250‑1 (MQ=255)

                                                                                            gAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTAt                                                                                                                                                 <  1:619941/250‑1 (MQ=255)

                                                                                             aaGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTc                                                                                                                                               <  1:49753/251‑1 (MQ=255)

                                                                                             aaGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTc                                                                                                                                               <  1:671467/251‑1 (MQ=255)

                                                                                                     ccgcACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGa                                                                                                                                       <  1:58295/251‑1 (MQ=255)

                                                                                                              tCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATAc                                                                                                                                                                                                               <  1:539174/170‑1 (MQ=255)

                                                                                                                   ggATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGc                                                                                                                                                                                                                                     >  1:484267/1‑143 (MQ=255)

                                                                                                                   ggATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGc                                                                                                                                                                                                                    <  1:793810/160‑1 (MQ=255)

                                                                                                                   ggATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAAc                                                                                                                                                       >  1:150336/1‑221 (MQ=255)

                                                                                                                        aaCAAATTCTGTTAGATCTTATCTTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAg                                                                                                                                                                                                                     <  1:487028/154‑1 (MQ=255)

                                                                                                                                cTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAg                                                                                                                                                                                                                     <  1:67251/146‑1 (MQ=255)

                                                                                                                                cTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCggg                                                                                                                                                               <  1:2867/200‑1 (MQ=255)

                                                                                                                                cTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAg                                                                                                              >  1:569621/1‑249 (MQ=255)

                                                                                                                                cTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAg                                                                                                              >  1:861636/1‑249 (MQ=255)

                                                                                                                                                          cTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATAttt                                                                                  <  1:872035/251‑1 (MQ=255)

                                                                                                                                                          cTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATAttt                                                                                  <  1:374935/251‑1 (MQ=255)

                                                                                                                                                           tCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATAtttt                                                                                 >  1:19714/1‑251 (MQ=255)

                                                                                                                                                                gggCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATAtt                                                                                   >  1:868725/1‑244 (MQ=255)

                                                                                                                                                                     gCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGa                                                                                                                                                                                                                  >  1:332530/1‑112 (MQ=255)

                                                                                                                                                                     gCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAAc                                                                                                                                                       <  1:860890/171‑1 (MQ=255)

                                                                                                                                                                     gCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCacac                                                                                           <  1:875902/231‑1 (MQ=255)

                                                                                                                                                                     gCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTAAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAgg                                                                        >  1:734811/1‑250 (MQ=255)

                                                                                                                                                                          gtgGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGt                                                                                                                                                                          <  1:869345/147‑1 (MQ=255)

                                                                                                                                                                            ggCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCATCGCCAAGAACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATAtt                                                                                   <  1:308052/232‑1 (MQ=255)

                                                                                                                                                                                  cGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGc                                                                                                             <  1:370035/200‑1 (MQ=255)

                                                                                                                                                                                   ggTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAg                                                                                                                                    >  1:373934/1‑176 (MQ=255)

                                                                                                                                                                                    gTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAAGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACg                                                          >  1:439968/1‑249 (MQ=255)

                                                                                                                                                                                           cGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTcaaac                                                 <  1:214768/251‑1 (MQ=255)

                                                                                                                                                                                           cGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTcaaac                                                 <  1:632709/251‑1 (MQ=255)

                                                                                                                                                                                           cGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTcaaac                                                 <  1:641315/251‑1 (MQ=255)

                                                                                                                                                                                           cGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTcaaac                                                 <  1:527520/251‑1 (MQ=255)

                                                                                                                                                                                              aGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGAc                                                           >  1:611307/1‑238 (MQ=255)

                                                                                                                                                                                                 ggTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAg                                            >  1:850486/1‑250 (MQ=255)

                                                                                                                                                                                                  gTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAAc                                                                                                                                                       <  1:636243/142‑1 (MQ=255)

                                                                                                                                                                                                      cTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAGCGAAt                                       >  1:100763/1‑250 (MQ=255)

                                                                                                                                                                                                                   ggAGATTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAg                                            <  1:470560/232‑1 (MQ=255)

                                                                                                                                                                                                                    gagaTTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAGCGAATCGGGGTAAATg                            <  1:480849/247‑1 (MQ=255)

                                                                                                                                                                                                                    gagaTTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAGCGAATCGGGGTAAATg                            <  1:296879/247‑1 (MQ=255)

                                                                                                                                                                                                                    gagaTTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAGCGAATCGGGGTAAATGAGc                         <  1:259061/250‑1 (MQ=255)

                                                                                                                                                                                                                      gaTTAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAg                                                                                                                                                                                        >  1:142713/1‑89 (MQ=255)

                                                                                                                                                                                                                        ttAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAAccc                                                                >  1:225876/1‑207 (MQ=255)

                                                                                                                                                                                                                        ttAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAGCGAATCGGGGTAAATg                            >  1:628261/1‑243 (MQ=255)

                                                                                                                                                                                                                        ttAATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAGCGAATCGGGGTAAATGAGCAAAt                     >  1:752359/1‑250 (MQ=255)

                                                                                                                                                                                                                           aTACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGc                                                                                                                                            >  1:796609/1‑128 (MQ=255)

                                                                                                                                                                                                                           aTACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACg                                                          >  1:327023/1‑210 (MQ=255)

                                                                                                                                                                                                                             acacAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACGCCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAGCGAATCGGGGTAAATGAGCAAATCATGGc               <  1:741963/251‑1 (MQ=255)

                                                                                                                                                                                                                             acacAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTg                                                                                >  1:866477/1‑186 (MQ=255)

                                                                                                                                                                                                                             acacAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAGCGAATCGGGGTAAATGAGCAAATCATgg                >  1:610722/1‑250 (MQ=255)

                                                                                                                                                                                                                                  aGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAGCGAATCGGGGTAAATGAGCAAATCATGGCAAAAg          >  1:284807/1‑251 (MQ=255)

                                                                                                                                                                                                                                   gTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAGCGAATCGGGGTAAATg                            <  1:864049/232‑1 (MQ=255)

                                                                                                                                                                                                                                     cTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAGCGAATCGGGGTAAATGAGCAAATCATGGCAAAAgggg       <  1:218232/251‑1 (MQ=255)

                                                                                                                                                                                                                                        cGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAGCGAATCGGGGTAAATg                            <  1:314710/227‑1 (MQ=255)

                                                                                                                                                                                                                                         gCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAGCGAATCGGGGTAAATGAGCAAATCATGGCAAAAGGGGAGaa   >  1:620964/1‑251 (MQ=255)

                                                                                                                                                                                                                                          ccAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAGCGAATCGGGGTAAATGAGCAAATCATGGCAAAAGGGGAGAAt  <  1:15161/251‑1 (MQ=255)

                                                                                                                                                                                                                                          ccAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAGCGAATCGGGGTAAATGAGCAAATCATGGCAAAAGGGGAGAAt  <  1:414153/251‑1 (MQ=255)

GCATCAAACAGGGGACGGAATTGCCCTATATTCTGGTGAACGAAAAAAGCGGTTCGCAAAGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCTTATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCGTACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTAATACACAAGTCTTCGCCAAGGATGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGATAAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCTGGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAGCGAATCGGGGTAAATGAGCAAATCATGGCAAAAGGGGAGAAT  >  1/1256457‑1256941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`