Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	80,911	T→C	N47D (AAC→GAC)	unknown ←	Stringent starvation protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	80,911	0	T	C	100.0%	181.2 / NA	52	N47D (AAC→GAC)	unknown	Stringent starvation protein B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/25);  total (27/25)

CTTTATCTACGGCATTTTCAAAACCTGTCGGTTGTTCTTCTGCCTGCTCAGGGTGTTCATAAGCGGCTTCAGGTTCAAACATCACACCGTCACCATTTTCACGGGCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTTTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTATATTCCATGAGATGACCTATTATTTTTCATCCATCAGATTTTTCGGCGCTAATTCGCCCACGGATTGCAGGAAAGAATCCCGTTCAAACACGCGGGTCATATAG  >  1/80665‑81145                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           cTTTATCTACGGCATTTTCAAAACCTGTCGGTTGTTCTTCTGCCTGCTCAGGGTGTTCATAAGCGGCTTCAGGTTCAAACATCACACCGTCACCATTTTCACGGGCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTg                                                                                                                                                                                                                                        >  1:683870/1‑251 (MQ=255)     aTCTACGGCATTTTCAAAACCTGTCGGTTGTTCTTCTGCCTGCTCAGGGTGTTCATAAGCGGCTTCAGGTTCAAACATCACACCGTCACCATTTTCACGGGCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACAt                                                                                                                                                                                                                                    <  1:714831/251‑1 (MQ=255)     aTCTACGGCATTTTCAAAACCTGTCGGTTGTTCTTCTGCCTGCTCAGGGTGTTCATAAGCGGCTTCAGGTTCAAACATCACACCGTCACCATTTTCACGGGCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACAt                                                                                                                                                                                                                                    <  1:859057/251‑1 (MQ=255)                       aCCTGTCGGTTGTTCTTCTGCCTGCTCAGGGTGTTCATAAGCGGCTTCAGGTTCAAACATCACACCGTCACCATTTTCACGGGCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTa                                                                                                                                                                                                                  >  1:133685/1‑251 (MQ=255)                                   ttcttcTGCCTGCTCAGGGTGTTCATAAGCGGCTTCAGGTTCAAACATCACACCGTCACCATTTTCACGGGCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATAc                                                                                                                                                                                                       <  1:827414/250‑1 (MQ=255)                                    tcttctGCCTGCTCAGGGTGTTCATAAGCGGCTTCAGGTTCAAACATCACACCGTCACCATTTTCACGGGCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGt                                                                                                                                                                                                     <  1:405692/251‑1 (MQ=255)                                              gcTCAGGGTGTTCATAAGCGGCTTCAGGTTCAAACATCACACCGTCACCATTTTCACGGGCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTAc                                                                                                                                                                                            >  1:769875/1‑250 (MQ=255)                                                        ttCATAAGCGGCTTCAGGTTCAAACATCACACCGTCACCATTTTCACGGGCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATAc                                                                                                                                                                                  <  1:363077/250‑1 (MQ=255)                                                                             aaaCATCACACCGTCACCATTTTCACGGGCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTAGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCAc                                                                                                                                                                                         >  1:137559/1‑222 (MQ=255)                                                                               aCATCACACCGTCACCATTTTCACGGGCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTaa                                                                                                                                                           >  1:546086/1‑250 (MQ=255)                                                                                     cacCGTCACCATTTTCACGGGCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCg                                                                                                                                                    >  1:524470/1‑251 (MQ=255)                                                                                       ccGTCACCATTTTCACGGGCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGta                                                                                                                                                  >  1:721391/1‑251 (MQ=255)                                                                                            aCCATTTTCACGGGCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAg                                                                                                                                             <  1:321133/251‑1 (MQ=255)                                                                                             ccATTTTCACGGGCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTGCCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGc                                                                                                                                            <  1:811040/251‑1 (MQ=255)                                                                                                    cACGGGCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTtaat                                                                                                                                     <  1:734414/251‑1 (MQ=255)                                                                                                        ggCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATAc                                                                                                                                                                                  <  1:878811/202‑1 (MQ=255)                                                                                                        ggCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTTGTTATCAATTAACCAATCGTAATAAGCCCTTaataaat                                                                                                                                 >  1:362044/1‑251 (MQ=255)                                                                                                        ggCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTaataaat                                                                                                                                 >  1:2907/1‑251 (MQ=255)                                                                                                        ggCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTaataaat                                                                                                                                 >  1:66589/1‑251 (MQ=255)                                                                                                                 cGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATAc                                                                                                                                                                                                       >  1:139850/1‑172 (MQ=255)                                                                                                                 cGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCt                                                                                                                                                                                              <  1:123095/181‑1 (MQ=255)                                                                                                                                  gCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCg                                                                                                                          >  1:125497/1‑232 (MQ=255)                                                                                                                                   cGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTatat                                                                                                       <  1:122007/250‑1 (MQ=255)                                                                                                                                     gAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGt                                                                                                                                                                                                     <  1:136767/154‑1 (MQ=255)                                                                                                                                     gAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATAc                                                                                                                                                                                  <  1:139576/173‑1 (MQ=255)                                                                                                                                     gAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAAcc                                                                                                                                                         <  1:360580/198‑1 (MQ=255)                                                                                                                                       atatAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTatat                                                                                                       <  1:205963/246‑1 (MQ=255)                                                                                                                                         ataAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTATATTCCATga                                                                                                >  1:527396/1‑251 (MQ=255)                                                                                                                                         ataAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTATATTCCATga                                                                                                >  1:145744/1‑251 (MQ=255)                                                                                                                                          taAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACa                                                                                                                                                                                                                                     >  1:756586/1‑117 (MQ=255)                                                                                                                                          taAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCg                                                                                                                          >  1:49306/1‑224 (MQ=255)                                                                                                                                          taAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTATATTCCATgag                                                                                               >  1:367532/1‑251 (MQ=255)                                                                                                                                               atCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCGAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAgggg                                                                                                                            >  1:502990/1‑217 (MQ=255)                                                                                                                                                tCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTc                                                                                                                                                                                                                                >  1:838578/1‑116 (MQ=255)                                                                                                                                                tCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTaataaata                                                                                                                                <  1:45533/212‑1 (MQ=255)                                                                                                                                                  cTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTATATTc                                                                                                     >  1:94828/1‑237 (MQ=255)                                                                                                                                                       acacacCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTATATTCCATGAGATGACCTattatt                                                                                  <  1:188188/251‑1 (MQ=255)                                                                                                                                                          caccacGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATAc                                                                                                                                                                                                       <  1:698676/131‑1 (MQ=255)                                                                                                                                                          caccacGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTATATTCCATGAGATGACCtattat                                                                                   >  1:891644/1‑247 (MQ=255)                                                                                                                                                          caccacGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTATATTCCATGAGATGACCtattat                                                                                   >  1:483669/1‑247 (MQ=255)                                                                                                                                                                        gcgTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTATATTCCATGAGATGACCTATTATTTTTCATCCATCAGAttt                                                                 >  1:733396/1‑251 (MQ=255)                                                                                                                                                                                        aaTCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGAc                                                                                                                                                                                                                                      <  1:652088/70‑1 (MQ=255)                                                                                                                                                                                               aGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAAcc                                                                                                                                                         >  1:689718/1‑140 (MQ=255)                                                                                                                                                                                                gTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTATATTCCATGAGATGACCTATTATTTTTCATCCATCAGATTTTTCGGCGCTAATTCGCCCACGGAt                                         >  1:543569/1‑251 (MQ=255)                                                                                                                                                                                                       gTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTATATTc                                                                                                     <  1:811292/184‑1 (MQ=255)                                                                                                                                                                                                       gTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTATATTCCATGAGATGACCTATTATTTTTCATCCATCAGATTTTTCGGCGCTAATTCGCCCACGGATTGCAGGa                                  >  1:777524/1‑251 (MQ=255)                                                                                                                                                                                                       gTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAACTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTATATTCCATgaga                                                                                              <  1:228561/191‑1 (MQ=255)                                                                                                                                                                                                               ccGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGc                                                                                                                                            <  1:78370/137‑1 (MQ=255)                                                                                                                                                                                                               ccGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTATATTCCATGAGATGACCTATTATTTTTCATCCATCAGATTTTTCGGCGCTAATTCGCCCACGGATTGCAGGAAAGAATcc                          <  1:888453/251‑1 (MQ=255)                                                                                                                                                                                                                              gACAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTATATTCCATGAGATGACCTATTATTTTTCATCCATCAGATTTTTCGGCGCTAATTCGCCCACGGATTGCAGGAAAGAATcc                          >  1:350985/1‑236 (MQ=255)                                                                                                                                                                                                                                cAAATTCAAAACGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTATATTCCATGAGATGACCTATTATTTTTCATCCATCAGATTTTTCGGCGCTAATTCGCCCACGGATTGCAGGAAAGAATCCCGTTCAAACACGCGGGt         <  1:755145/251‑1 (MQ=255)                                                                                                                                                                                                                                       aaaaCGATTTGTCCGTCTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTATATTCCATGAGATGACCTATTATTTTTCATCCATCAGATTTTTCGGCGCTAATTCGCCCACGGATTGCAGGAAAGAATCCCGTTCAAACACGCGGGTCATATAg  <  1:185084/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           CTTTATCTACGGCATTTTCAAAACCTGTCGGTTGTTCTTCTGCCTGCTCAGGGTGTTCATAAGCGGCTTCAGGTTCAAACATCACACCGTCACCATTTTCACGGGCATAAATCGCTAATGCAGCGCCAAGCGGAATATAAATATCCTGAGACACACCACGAAAACGCGCGTTAAATTGAATAAAATCATTAGTCAATTGTAAATTACCGGTGGCATTGGCAGACAAATTCAAAACGATTTGTCCGTTTTTGACATACTCCACCGGCACTTTTACGCCCCAATACGTGGCATCTACCACTAAATACGGCGTGAAGTCGTTATCAATTAACCAATCGTAATAAGCCCTTAATAAATAGGGGCGTTTCGGTGAGGATTTATATTCCATGAGATGACCTATTATTTTTCATCCATCAGATTTTTCGGCGCTAATTCGCCCACGGATTGCAGGAAAGAATCCCGTTCAAACACGCGGGTCATATAG  >  1/80665‑81145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑