Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	74,214	A→C	K34T (AAA→ACA)	unknown →	LsrR, transcriptional repressor of lsr operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	74,214	0	A	C	100.0%	169.0 / NA	49	K34T (AAA→ACA)	unknown	LsrR, transcriptional repressor of lsr operon
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/24);  total (25/24)

TGATCTCCATCATGCTTTTACAAAAAAACGGTAAGAACAATTGATAATAATAAAAAACAAATGAATAATACCCAAAATCAATAAAAACAAATAAATCATATGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGAAACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGAT‑TAAAGCATATACGTATTCTGCCAACGCTGGAACAACATGAAATCAATGAGCGATTAGGCGTCGGCGCGGCACAAATGCTGATG  >  1/74015‑74452                                                                                                                                                                                                        |                                                                                                                                                                                                                                                tGATCTCCATCATGCTTTTACAAAAAAACGGTAAGAACAATTGATAATAATAAAAAACAAATGAATAATACCCAAAATCAATAAAAACAAATAAATCATATGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTGTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATcc                                                                                                                                                                                                <  1:196983/249‑1 (MQ=255)       ccATCATGCTTTTACAAAAAAACGGTAAGAACAATTGATAATAATAAAAAACAAATGAATAATACCCAAAATCAATAAAAACAAATAAATCATATGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTa                                                                                                                                                                                          >  1:232059/1‑249 (MQ=255)                                    aaCAATTGATAATAATAAAAAACAAATGAATAATACCCAAAATCAATAAAAACAAATAAATCATATGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCTCGAATTGCCTGTTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCTGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCAatatta                                                                                                                                                           <  1:165169/251‑1 (MQ=255)                                        aTTGATAATAATAAAAAACAAATGAATAATACCCAAAATCAATAAAAACAAATAAATCATATGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGt                                                                                                                                                       >  1:748409/1‑251 (MQ=255)                                           gATAATAATAAAAAACAAATGAATAATACCCAAAATCAATAAAAACAAATAAATCATATGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCa                                                                                                                                                    >  1:508909/1‑251 (MQ=255)                                                               gAATAATACCCAAAATCAATAAAAACAAATAAATCATATGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAg                                                                                                                                                        >  1:508725/1‑227 (MQ=255)                                                               gAATAATACCCAAAATCAATAAAAACAAATAAATCATATGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCg                                                                                                                                >  1:69773/1‑251 (MQ=255)                                                                           aaaTCAATAAAAACAAATAAATCATATGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTctggaa                                                                                                                     >  1:157113/1‑250 (MQ=255)                                                                               cAATAAAAACAAATAAATCATATGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTctggaactgga                                                                                                                >  1:850917/1‑251 (MQ=255)                                                                                     aaaCAAATAAATCATATGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGt                                                                                                          >  1:675185/1‑251 (MQ=255)                                                                                        cAAATAAATCATATGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTg                                                                                                         >  1:619191/1‑249 (MQ=255)                                                                                        cAAATAAATCATATGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTg                                                                                                         >  1:603655/1‑249 (MQ=255)                                                                                              aatCATATGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCAttatta                                                                                                                                                           >  1:121870/1‑193 (MQ=255)                                                                                              aatCATATGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTacaaca                                                                                                 >  1:209695/1‑251 (MQ=255)                                                                                                  atatGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAacac                                                                                             <  1:248513/251‑1 (MQ=255)                                                                                                  atatGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAacac                                                                                             <  1:320208/251‑1 (MQ=255)                                                                                                     tGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACCGGAAGAAGTGCTACAACAACACTTc                                                                                          <  1:313795/251‑1 (MQ=255)                                                                                                           aaTTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCa                                                                                                                                                                 >  1:608871/1‑174 (MQ=255)                                                                                                           aaTTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTctggaac                                                                                                                    <  1:727574/219‑1 (MQ=255)                                                                                                              tAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGAT‑TAAAg                                                                                <  1:856161/251‑1 (MQ=255)                                                                                                                    cGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGaa                                                                                                                                                                                    >  1:96665/1‑146 (MQ=255)                                                                                                                    cGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGAT‑TAAAGCAtata                                                                          >  1:349564/1‑251 (MQ=255)                                                                                                                       aTAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGAT‑TAAAGCATATACg                                                                        <  1:160932/250‑1 (MQ=255)                                                                                                                       aTAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGAT‑TAAAGCATATACg                                                                        <  1:7278/250‑1 (MQ=255)                                                                                                                         aaTCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGAT‑TAAAGCATATACGTAt                                                                     <  1:294175/251‑1 (MQ=255)                                                                                                                          aTCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCAttatta                                                                                                                                                           >  1:83405/1‑165 (MQ=255)                                                                                                                          aTCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGAT‑TAAAGCATATACGTAtt                                                                    >  1:37537/1‑251 (MQ=255)                                                                                                                           tCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTac                                                                                                     >  1:465671/1‑218 (MQ=255)                                                                                                                            cAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGAT‑TAAAGcc                                                                              <  1:96638/239‑2 (MQ=255)                                                                                                                                aaaCGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGAT‑TAAAGCATATACGTATTCTGc                                                                <  1:783139/249‑1 (MQ=255)                                                                                                                                    gATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAacac                                                                                             <  1:168456/217‑1 (MQ=255)                                                                                                                                     aTCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAg                                                                                                                                                        <  1:539790/157‑1 (MQ=255)                                                                                                                                     aTCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTacaac                                                                                                  >  1:558619/1‑211 (MQ=255)                                                                                                                                     aTCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTc                                                                                          <  1:500969/219‑1 (MQ=255)                                                                                                                                          aaGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGAT‑TAAAGCATATACGTATTCTGCCAACGCTGGaac                                                    <  1:578850/251‑1 (MQ=255)                                                                                                                                                     aagaagaaTTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGa                                                                                       <  1:748175/206‑1 (MQ=255)                                                                                                                                                       gaagaaTTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGAT‑TAAAGCATATACg                                                                        <  1:865373/218‑1 (MQ=255)                                                                                                                                                       gaagaaTTGTTGGCACGAATTGCCTGGTTTTATTATCACGAAAACCTGACACAAAGCGAAATCGGCGACAAACTAAAACTGCCGAGTTTAAAAGAATCCAGGCTACTAGAAAAAGGGCGTCAGTCCGGCATCACTAAAGTGCAAATCAACTCCCGCATTATCGGTTGTCTGGAACGAGAAGAAGAAAAAGAACAACACATCC‑ATCTAAAGCAAAAAAGTATTCTGCAAACGCTGGaacaaaaacaactcaa                                       >  1:648309/1‑240 (MQ=255)                                                                                                                                                                  ggCACGAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTg                                                                                                                            <  1:168611/156‑1 (MQ=255)                                                                                                                                                                       gAATTGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTacaac                                                                                                  <  1:250249/177‑1 (MQ=255)                                                                                                                                                                          ttGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGAT‑TAAAGCATATACGt                                                                       >  1:476018/1‑200 (MQ=255)                                                                                                                                                                           tGCCTGGTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGAT‑TAAAGCATATACGTATTCTGCCAACGCTGGAACAACATGAAATCAATGAGCGATTAGGCGTCGgcg                   <  1:248373/251‑1 (MQ=255)                                                                                                                                                                                 gTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGAT‑TAAAGCATATACGTATTCTGCCAACGCTGGAACAACATGAAATCAATGAGCGATTAGGCGTCGGCGCGGca              >  1:398216/1‑250 (MQ=255)                                                                                                                                                                                 gTTTTATTATCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGAT‑TAAAGCATATACGTATTCTGCCAACGCTGGAACAACATGAAATCAATGAGCGATTAGGCGTCGGCGCGGca              >  1:459442/1‑250 (MQ=255)                                                                                                                                                                                        tatCACGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCAAT‑TAAAGCATATACGTATTCCGCCAACGCTGGAACAACATGAAATCAATg                                     >  1:424239/1‑220 (MQ=255)                                                                                                                                                                                            aCGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGAT‑TAAAGCATATACGTATTCTGCCAACGCTGGAACAACATGAAATCAATGAGCGATTAGGCGTCGGCGCGGCACAAATGCTGATg  <  1:322575/251‑1 (MQ=255)                                                                                                                                                                                             cGATAACCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTc                                                                                          >  1:321370/1‑163 (MQ=255)                                                                                                                                                                                                  aCCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTc                                                                                                                                                                      <  1:337711/82‑1 (MQ=255)                                                                                                                                                                                                  aCCTGACACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGAT‑TAAAGCATATACGTATTCTGCCAACGCTGGAACAACATGAAATCAATGAGCGATTAgg                           <  1:181752/220‑1 (MQ=255)                                                                                                                                                                                                        |                                                                                                                                                                                                                                                TGATCTCCATCATGCTTTTACAAAAAAACGGTAAGAACAATTGATAATAATAAAAAACAAATGAATAATACCCAAAATCAATAAAAACAAATAAATCATATGAACGAATTAATGACGGATAATCAAGAAACGATCCAAAGTTTCGGCGAAGAAGAATTGTTGGCACGAATTGCCTGGTTTTATTATCACGATAACCTGAAACAAAGCGAAATCGGCGACAGACTAAAACTGCCGCGTTTAAAAGTATCCAGGCTACTGGAAAAAGGGCGTCAGTCCGGCATTATTAAAGTGCAAATCAACTCCCGCTTTACCGGTTGTCTGGAACTGGAAGAAGTGCTACAACAACACTTCCGAT‑TAAAGCATATACGTATTCTGCCAACGCTGGAACAACATGAAATCAATGAGCGATTAGGCGTCGGCGCGGCACAAATGCTGATG  >  1/74015‑74452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑