| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 960,994 | A→C | S698R (AGT→CGT) | unknown → | VgrG protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 960,994 | 0 | A | C | 98.7% | 249.7 / NA | 79 | S698R (AGT→CGT) | unknown | VgrG protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (30/48); total (31/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATGAATACCTTAGTGCTCAATGACCGTGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCAAGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCTTCGGATGACGGTGGCGACGGAGGTGCTCGTGGCGGTGGTGCTGGTGGCGGTTCTGGAGGTGGCGGTGGTGGCGCTTCTGGTGGTAACGATTCTAGTGGTGCTGAGACTGAGAATTCTG > 1/960747‑961240 | tatGAATACCTTAGTGCTCAATGACCGTGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTc < 1:286504/251‑1 (MQ=255) aTACCTTAGTGCTCAATGACCGTGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGG‑GAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTAccc < 1:813436/251‑1 (MQ=255) tACCTTAGTGCTCAATGACCGTGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTAccc < 1:306027/251‑1 (MQ=255) tACCTTAGTGCTCAATGACCGTGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTAccc < 1:54656/251‑1 (MQ=255) tACCTTAGTGCTCAATGACCGTGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTAccc < 1:355145/251‑1 (MQ=255) ccTTAGTGCTCAATGACCGTGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAAACCAAACCATTGACGAGGCGCAAAACATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGGCCGGGGGCAATGACATCCAAAGCGGATAATGTGATATTTTAGA‑CCGCCGGGGCACGTCTTAcccc > 1:629857/1‑249 (MQ=255) tGCTCAATGACCGTGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAAACATTACTGTAGGCAAGGAGTATCAGTTAATCGCCAATAACGGGCCGGTGTCAATGACAACCAAGGCGGGTAATGGGGTATTTTAGA‑CCGCCGGGGCACGTCTTACCCTATTTaaa > 1:25782/1‑250 (MQ=255) gCTCAATGACCGTGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAAACCAAAACATTGACGAGGCGCAAAACATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAAAGGGCCGGGGGCAATGACATCCAAAGCGGGTAATGTGATATTTTAGA‑CCGCCGGGGCACGTCTTACCCTATTTAAGa > 1:55209/1‑251 (MQ=255) cTCAATGACCGTGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGaa < 1:240091/251‑1 (MQ=255) cTCAATGACCGTGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGaa < 1:604256/251‑1 (MQ=255) cTCAATGACCGTGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGaa < 1:596517/251‑1 (MQ=255) cTCAATGACCGTGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAAACATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGGGCACGTCTTACCCTATTTAAGaa > 1:656621/1‑251 (MQ=255) cTCAATGACCGTGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGACAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGaa < 1:834518/251‑1 (MQ=255) cAATGACCGTGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGaa < 1:619987/249‑1 (MQ=255) cGTGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAatat < 1:464649/251‑1 (MQ=255) gTGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTAccc < 1:756546/231‑1 (MQ=255) tGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGGGAGGAAACCCAAACCATTGACAAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAGCGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTc < 1:471447/224‑1 (MQ=255) tGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTc < 1:814863/251‑1 (MQ=255) ccACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAAACCAAACCATTGACGAGGCGCAAAACATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGGCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGGCTTACCCTATTTTAGaa > 1:59216/1‑238 (MQ=255) cACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTAcc < 1:855305/226‑1 (MQ=255) cACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAAc < 1:661780/251‑1 (MQ=255) cGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAGAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTggaagggaag < 1:874321/250‑1 (MQ=255) cGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTggaagggaag < 1:2363/250‑1 (MQ=255) cGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTggaagggaag < 1:477543/250‑1 (MQ=255) gAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCCAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCCATAACGGTCCGGGGTCAATGACATCCCAGGCGGATAATGTGATATTTTAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAAAGGGAATATTCAAc > 1:844927/1‑236 (MQ=255) gAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAAACCAAACCATTGACGAGGCGCAAATCATTACTGTAGGGAAGGATTATCAGGTAATCGCCAATAAAGGGCCGGGGTCAAAGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGGGGACGTCTTACCCCATTTAAGAAAGGGAATATTTAAATGGAAGGGAAGGCgg > 1:484255/1‑251 (MQ=255) caAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTTACGAGGCGCAAATCATTACTGTAGGCAAGGAATATCAGTTAATCGCCAATAAAGGTCCGGGGTCAATGACATCCAAAGCGGATAATGTGATATTTTAGACCCCCCGGGGC‑AGTCTTACCCTATTTAAGAACGGGGAAATTTCACTGGAAGGGAAGTCGgtgggtt > 1:866153/1‑247 (MQ=255) caAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTc < 1:814443/200‑1 (MQ=255) aaaCCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTaa < 1:632322/250‑1 (MQ=255) aaaCCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTaa < 1:264906/250‑1 (MQ=255) aaaCCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTaa < 1:522404/250‑1 (MQ=255) aCCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATgg < 1:754688/251‑1 (MQ=255) aCCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATgg < 1:217607/251‑1 (MQ=255) tCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAg < 1:460613/251‑1 (MQ=255) aacggAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAAACCAAACCATTGACGAGGGGCAAAACATTACTGTAGGCAAGGATTATCAGTTAATCGCCAAAAAAGGTCCCGTGTCAATGACATCCAAGGCGGGTAATGTGATATTTTAGA‑CCGCCGGTGGACGGCTTACCCCATTTAAAAAAGGGAATATTTAAATGGAAGGGGAGTCGGGGGGTATTAATGGTAAGCTCATTGa > 1:298933/5‑250 (MQ=255) aaGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATgg < 1:613014/232‑1 (MQ=255) aaGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATgg < 1:71305/232‑1 (MQ=255) aaGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAAACCAAAACATTTACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAAAGGGCCGGGGTCAATGACATCCAAGGCGGGTAATGTGATATTTGAGA‑CCGCCCGGGCACGTCTTACCCTATTTAAGAAAGGGAATATTTAAATGGAAGGGAAGGCGGGGTGTATTTATGGTAAGCTCATTGACTTGaaa > 1:691165/1‑250 (MQ=255) gTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAAACATTGACGAGGCGCAAAATATTACTTTAGGCAAGGACTATCAGTTAATCGCCAATAACGGTCCGGGGTCAATGAAATCCAAGGCGGATAAAGTGATATTTGAGA‑CCGCCGGGGCACGTCTTAACCTATTTAAGaa > 1:194143/1‑188 (MQ=255) gTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAAACCCAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAAAGGTCCGGGGTCAATGACATCCAAGGCGGGTAATGTGATATTTGAGA‑CCGCCGGTGCACGTc > 1:57234/1‑172 (MQ=255) ttAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATc < 1:895733/249‑1 (MQ=255) tgtCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGt < 1:742697/250‑1 (MQ=255) gTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTAttt < 1:460596/164‑1 (MQ=255) ttATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGTTATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGt < 1:763608/235‑1 (MQ=255) ataAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACtt < 1:274959/223‑1 (MQ=255) gaagTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTAccc < 1:461292/151‑1 (MQ=255) gTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTAt > 1:26778/1‑194 (MQ=255) ggCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAg > 1:827034/1‑248 (MQ=255) gagaGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTAACAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTc < 1:13600/250‑1 (MQ=255) aCCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTc > 1:581553/1‑154 (MQ=255) aaCCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTaa < 1:588377/177‑1 (MQ=255) ccATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGggc > 1:443535/1‑208 (MQ=255) ccATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCTTCGGAt > 1:408864/1‑251 (MQ=255) gcgcAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCTTCGGATGACGGTGGCGa > 1:786825/1‑251 (MQ=255) cAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTAtt < 1:373689/159‑1 (MQ=255) aTCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCTTCGGATGACGGTGGCGACGGAgg < 1:29691/251‑1 (MQ=255) tCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAGGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCTTCGGATGa > 1:612125/1‑235 (MQ=255) aCTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAAc < 1:159875/114‑1 (MQ=255) ggCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATc > 1:810786/1‑169 (MQ=255) gCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCTTCGGATGACGGTGGCGACGGAGGTGCTCGTGGCgg < 1:144845/250‑1 (MQ=255) gATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGaa > 1:200788/1‑101 (MQ=255) gATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCt > 1:300576/1‑210 (MQ=255) ttATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTAccc > 1:303252/1‑89 (MQ=255) cAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCTTCGGATGACGGTGGCGACGGAGGTGCTCGTGGCGGTGGTGc < 1:72960/245‑1 (MQ=255) ccAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTc > 1:673053/1‑134 (MQ=255) ccAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCTTCGGATGACGGTGGCGACGGAGGTGCTCGTGGCGGTGGGGCTGGTGGCGGGTCttgg > 1:31284/1‑248 (MQ=255) aTAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAg < 1:795303/80‑1 (MQ=255) tAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCTTCGGATGACGGTGGCGACGGAGGTGCTCGTGGCGGTGGTGGCGGTGGCGGTCCTggggggg > 1:292268/1‑246 (MQ=255) gTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTAtt < 1:462417/113‑1 (MQ=255) gTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGTGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCTTCGGATGACGGTGGCGACGGAGGTGCTCGTGGCGGTGGTGCTGGTGGGGGTTCTgggggggggggg > 1:831770/1‑241 (MQ=255) aTGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCTTCGGATGACGGTGGCGACGGAGGTGCTCGTGGCGGTGGGGCTGGTGGCGGGTCTGGGGGGGGCggggggggggcctt > 1:360290/1‑239 (MQ=255) gCGGATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCTTCGGATGACGGTGGCGACGGAGGTGCTCGTGGCGGTGGTGCTGGTGGCGGTTCTGGAGGTGGCGGTGGTGGCGCTTCTGGTGGTAACg < 1:433220/250‑1 (MQ=255) gATAATGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCTTCGGATGACGGTGGCGACGGAGGTGCTCGTGGCGGTGGGGCTGGGGGCGGGTTTGGGGGGGGCGGGGGGGGGGCTTCTGGTGGGAACGagtt > 1:783711/1‑248 (MQ=255) aTGTGATATTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTc < 1:511077/145‑1 (MQ=255) atatTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCTTCGGATGACGGTGGCGACGGAGGTGCTCGTGGCGGTGGGGCTGGGGGCGGGTCTGGGGGGGGGGGGGGGGGGGCTTCtggtggggaaggttttagtggtgg > 1:635457/1‑233 (MQ=255) atTTGAGA‑CCGCCGGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGa < 1:693962/98‑1 (MQ=255) ccgccgGTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCTTCGGATGACGGTGGCGACGGAGGTGCTCGTGGCGGTGGTGCTGGTGGCGGTTCTGGAGGTGGCGGTGGTGGCGCTTCTGGTGGTAACGATTCTAGTGGTGctgagactgag < 1:670984/251‑1 (MQ=255) gTGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCTTCGGATGACGGTGGCGACGGAGGTGCTCGTGGCGGTGGTGCTGGGGGCGGGTCTGGGGGGGGCGGGGGGGGGGCTTCTGGGGGGAACGGTTCTAGGGGGGCTGGGACTGAGaaat > 1:532842/1‑247 (MQ=255) tGCACGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCTTCGGATGACGGTGGCGACGGAGGTGCTCGTGGCGGTGGTGCTGGTGGCGGTTCTGGAGGTGGCGGTGGTGGCGCTTCTGGTGGTAACGATTCTAGTGGTGCTGAGACTGAGAATTCTg < 1:719087/251‑1 (MQ=255) | TATGAATACCTTAGTGCTCAATGACCGTGCCACTAATGTGGGCGGGAATCACAAAGAAACCGTCAAAGGCGAACAGAAAGTTAGTGTCGGCAAAGCCCGTTATAAAGAAGTGGCGGGAGAGGAAACCCAAACCATTGACGAGGCGCAAATCATTACTGTAGGCAAGGATTATCAGTTAATCGCCAATAACGGTCCGGTGTCAATGACATCCAAGGCGGATAATGTGATATTTGAGA‑CCGCCGGTGCAAGTCTTACCCTATTTAAGAACGGGAATATTCAACTGGAAGGGAAGTCGGTGTGTATTAATGGTAAGCTCATTGACTTGAATCCGGGTGGGGCGGCGACATCGAGTGCTGAAGCTGATTCTGCGCCGGCTTCGGATGACGGTGGCGACGGAGGTGCTCGTGGCGGTGGTGCTGGTGGCGGTTCTGGAGGTGGCGGTGGTGGCGCTTCTGGTGGTAACGATTCTAGTGGTGCTGAGACTGAGAATTCTG > 1/960747‑961240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |