Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	671,321	T→C	C40R (TGC→CGC)	unknown →	GTP‑binding protein Era

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	671,321	0	T	C	100.0%	260.8 / NA	78	C40R (TGC→CGC)	unknown	GTP‑binding protein Era
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/39);  total (39/39)

ATAATCAATTGTTCATCGTGGAATGTTCCATCCAAAATAGTGACCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCATGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGAACGACGATGATGAAATGGTGCTAAACAAACTGCGCAAAACCAAAGCGCCGGTG  >  1/671077‑671566                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      aTAATCAATTGTTCATCGTGGAATGTTCCATCCAAAATAGTGACCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAg                                                                                                                                                                                                                                                 >  1:170598/1‑251 (MQ=255)  tAATCAATTGTTCATCGTGGAATGTTCCATCCAAAATAGTGACCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAg                                                                                                                                                                                                                                                 >  1:177975/1‑250 (MQ=255)      cAATTGTTCATCGTGGAATGTTCCATCCAAAATAGTGACCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCaa                                                                                                                                                                                                                                            >  1:715896/1‑251 (MQ=255)        aTTGTTCATCGTGGAATGTTCCATCCAAAATAGTGACCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAac                                                                                                                                                                                                                                          <  1:526968/251‑1 (MQ=255)         ttGTTCATCGTGGAATGTTCCATCCAAAATAGTGACCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAacc                                                                                                                                                                                                                                         <  1:626369/251‑1 (MQ=255)         ttGTTCATCGTGGAATGTTCCATCCAAAATAGTGACCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAacc                                                                                                                                                                                                                                         <  1:415705/251‑1 (MQ=255)                tCGTGGAATGTTCCATCCAAAATAGTGACCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGc                                                                                                                                                                                                                                   >  1:102258/1‑250 (MQ=255)                       aTGTTCCATCCAAAATAGTGACCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCAt                                                                                                                                                                                                                           <  1:135013/251‑1 (MQ=255)                         gTTCCATCCAAAATAGTGACCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATtg                                                                                                                                                                                                                         >  1:480306/1‑251 (MQ=255)                         gTTCCATCCAAAATAGTGACCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATtg                                                                                                                                                                                                                         >  1:307738/1‑251 (MQ=255)                          ttCCATCCAAAATAGTGACCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATtg                                                                                                                                                                                                                         <  1:539982/250‑1 (MQ=255)                          ttCCATCCAAAATAGTGACCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATtg                                                                                                                                                                                                                         <  1:818566/250‑1 (MQ=255)                          ttCCATCCAAAATAGTGACCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATtg                                                                                                                                                                                                                         <  1:586915/250‑1 (MQ=255)                             catccaAAATAGTGACCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTgg                                                                                                                                                                                                                      <  1:820086/250‑1 (MQ=255)                              atccaAAATAGTGACCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGGATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACTTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTgggg                                                                                                                                                                                                                    >  1:706153/1‑250 (MQ=255)                                      tAGTGACCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAAc                                                                                                                                                                                                            <  1:219539/251‑1 (MQ=255)                                           aCCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAg                                                                                                                                                                                                       >  1:372179/1‑251 (MQ=255)                                            ccGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAgg                                                                                                                                                                                                      <  1:178952/251‑1 (MQ=255)                                            ccGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAgg                                                                                                                                                                                                      <  1:536666/251‑1 (MQ=255)                                               cACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTg                                                                                                                                                                                                    >  1:203703/1‑250 (MQ=255)                                                 cTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAacc                                                                                                                                                                                                                                         >  1:406334/1‑211 (MQ=255)                                                   tttATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAacc                                                                                                                                                                                                                                         >  1:508078/1‑209 (MQ=255)                                                   tttATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTAt                                                                                                                                                                                               >  1:682665/1‑251 (MQ=255)                                                        cGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGa                                                                                                                                                                                          <  1:19466/251‑1 (MQ=255)                                                        cGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGa                                                                                                                                                                                          <  1:546116/251‑1 (MQ=255)                                                          gTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGaaa                                                                                                                                                                                        >  1:351075/1‑251 (MQ=255)                                                                   ccAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAGCAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCGCCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTgg                                                                                                                                                                               <  1:671061/251‑1 (MQ=255)                                                                          tcgtAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAacc                                                                                                                                                                                                                                         <  1:276831/186‑1 (MQ=255)                                                                          tcgtAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACAccc                                                                                                                                                                         >  1:726117/1‑250 (MQ=255)                                                                               aaGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGt                                                                                                                                                                   <  1:160626/251‑1 (MQ=255)                                                                                    gAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCat                                                                                                                                                              >  1:742719/1‑251 (MQ=255)                                                                                     aaCAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAACCTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCat                                                                                                                                                              <  1:408103/250‑1 (MQ=255)                                                                                     aaCAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCat                                                                                                                                                              <  1:685628/250‑1 (MQ=255)                                                                                          gcggcggcAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTga                                                                                                                                                         <  1:839072/250‑1 (MQ=255)                                                                                          gcggcggcAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTga                                                                                                                                                         <  1:345266/250‑1 (MQ=255)                                                                                                          ttttACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAg                                                                                                                                                                                                       >  1:695411/1‑188 (MQ=255)                                                                                                                aaGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGcc                                                                                                                                  <  1:335027/251‑1 (MQ=255)                                                                                                                        aaTATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAAc                                                                                                                          <  1:764392/251‑1 (MQ=255)                                                                                                                            tCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACcgcg                                                                                                                      >  1:555318/1‑251 (MQ=255)                                                                                                                                       aacaacAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCcagcag                                                                                                            <  1:265045/250‑1 (MQ=255)                                                                                                                                                     aCTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGAtgt                                                                                             >  1:350462/1‑251 (MQ=255)                                                                                                                                                     aCTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGAtgt                                                                                             <  1:701443/251‑1 (MQ=255)                                                                                                                                                         aCTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTTCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAg                                                                                         <  1:439705/251‑1 (MQ=255)                                                                                                                                                         aCTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAg                                                                                         <  1:558090/251‑1 (MQ=255)                                                                                                                                                               gCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGc                                                                                                  >  1:556597/1‑236 (MQ=255)                                                                                                                                                               gCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTg                                                                                   >  1:723801/1‑251 (MQ=255)                                                                                                                                                                cTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTAc                                                                                                                                                                                   <  1:385308/154‑1 (MQ=255)                                                                                                                                                                 tttATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGAt                                                                                 <  1:160113/251‑1 (MQ=255)                                                                                                                                                                          aTCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGc                                                                                                  >  1:318420/1‑225 (MQ=255)                                                                                                                                                                          aTCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCgtggt                                                                        >  1:627347/1‑251 (MQ=255)                                                                                                                                                                              tcggtcgTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCat                                                                                                                                                              <  1:811376/161‑1 (MQ=255)                                                                                                                                                                                  tcgtcCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGc                                                                 <  1:589914/250‑1 (MQ=255)                                                                                                                                                                                    gtcCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGcac                                                               >  1:852035/1‑250 (MQ=255)                                                                                                                                                                                         aaTGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACt                                                          <  1:569887/250‑1 (MQ=255)                                                                                                                                                                                         aaTGTAGGCAAAGCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGTCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACt                                                          <  1:423863/250‑1 (MQ=255)                                                                                                                                                                                          aTGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCatat                                                                                                                                                            <  1:754342/151‑1 (MQ=255)                                                                                                                                                                                             tAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGaa                                                      <  1:635359/250‑1 (MQ=255)                                                                                                                                                                                             tAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGAac                                                     <  1:761498/251‑1 (MQ=255)                                                                                                                                                                                             tAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGAac                                                     <  1:433216/251‑1 (MQ=255)                                                                                                                                                                                             tAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGAac                                                     <  1:156343/251‑1 (MQ=255)                                                                                                                                                                                               ggCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGCGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGAacga                                                   >  1:837262/1‑251 (MQ=255)                                                                                                                                                                                                 cAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACt                                                          >  1:258952/1‑242 (MQ=255)                                                                                                                                                                                                 cAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGAacgacg                                                 >  1:629868/1‑251 (MQ=255)                                                                                                                                                                                                  aaaTCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATc                                                                                                     >  1:758046/1‑198 (MQ=255)                                                                                                                                                                                                   aaTCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACTGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGaa                                                      <  1:429837/244‑1 (MQ=255)                                                                                                                                                                                                    aTCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGAACGACgatg                                              >  1:23274/1‑251 (MQ=255)                                                                                                                                                                                                             gctgAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGAACGACGATGATGAAATgg                                     >  1:483472/1‑251 (MQ=255)                                                                                                                                                                                                             gctgAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGAACGACGATGATGAAATgg                                     >  1:733230/1‑251 (MQ=255)                                                                                                                                                                                                                gAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGa                                                                                                >  1:379304/1‑189 (MQ=255)                                                                                                                                                                                                                gAATAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGAACGACGATGATGAAATgg                                     >  1:669859/1‑248 (MQ=255)                                                                                                                                                                                                                 aaTAAAATCCTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGaaaaa                                                                                                                                                  >  1:895676/1‑138 (MQ=255)                                                                                                                                                                                                                         ccTCGGACAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGAACGACGATGATGAAATGGTGCTaaacaaac                         <  1:519873/251‑1 (MQ=255)                                                                                                                                                                                                                                cAAAAAATCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGAACGACGATGATGAAATGGTGCTaaac                             >  1:578800/1‑240 (MQ=255)                                                                                                                                                                                                                                      aTCTCCATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGAACGACGATGATGAAATGGTGCTAAACAAACTGCGCAAAACCaa            >  1:640919/1‑251 (MQ=255)                                                                                                                                                                                                                                          ccATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGc                                                                 >  1:591800/1‑194 (MQ=255)                                                                                                                                                                                                                                          ccATCACCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGAACGACGATGATGAAATGGTGCTAAACAAACTGCGCAAAACCAAAgcg        >  1:702022/1‑251 (MQ=255)                                                                                                                                                                                                                                               aCCTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGAACGACGATGATGAAATGGTGCTAAACAAACTGCGCAAAACCAAAGCGCCggt   >  1:428227/1‑251 (MQ=255)                                                                                                                                                                                                                                                ccTCACGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGAACGACGATGATGAAATGGTGCTAAACAAACTGCGCAAAACCAAAGCGCCggtg  >  1:139437/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ATAATCAATTGTTCATCGTGGAATGTTCCATCCAAAATAGTGACCGCACTTTTATCGGTAAAGGCTCCAGTCGTCGTAAAGCGGAACAGGCGGCGGCAGAACAAATTTTACAAGAGTTGAATATCAAATGAGCGAACAACAAAACCAAACTTACTGCGGCTTTATTGCTATCGTCGGTCGTCCGAATGTAGGCAAATCCACCCTGCTGAATAAAATCCTCGGACAAAAAATCTCCATCACCTCATGCAAAGCCCAAACCACCCGCCACCGCATTGTGGGCATTAAAACCGAAGGTGCGTATCAAGAAATTTACGTGGACACCCCGGGGTTGCATATTGAAGAAAAACGCGCCATCAATCGCCTGATGAACCGCGCCGCCAGCAGTGCTATCAGCGATGTGGAGTTGGTGATTTTCGTGGTGGACGGCACACACTGGAACGACGATGATGAAATGGTGCTAAACAAACTGCGCAAAACCAAAGCGCCGGTG  >  1/671077‑671566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑