Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	627,978	T→G	Q2P (CAA→CCA)	unknown ←	UPF0234 protein Yitk

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	627,978	0	T	G	100.0%	271.7 / NA	78	Q2P (CAA→CCA)	unknown	UPF0234 protein Yitk
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (42/36);  total (42/36)

CGGGAATGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATTGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGCAAGATCGTTGGCGCCGTCGCCGATGGCAACGGTGTTTTCGGCGGCAATGTGAAATTGTTGCGCAAGTTGTTGTAAGATTTGCGCTTTGTAT  >  1/627736‑628221                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    cggGAATGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGt                                                                                                                                                                                                                                              <  1:151310/250‑1 (MQ=255)   ggAATGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGttt                                                                                                                                                                                                                                            <  1:777473/250‑1 (MQ=255)      aTGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACt                                                                                                                                                                                                                                         >  1:194846/1‑250 (MQ=255)      aTGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTc                                                                                                                                                                                                                                        <  1:802926/251‑1 (MQ=255)      aTGTACAGGGAGTTGGATTAAATTTTGCGCTTGATGAAGGCGTTAGGCAGAATATTGATAAGTTGTTCCACCTGGAAATTCGATTCGCTGCTGACTTTGATGGTTTCTTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTTCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTc                                                                                                                                                                                                                                        <  1:802908/251‑1 (MQ=255)       tGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTcc                                                                                                                                                                                                                                       <  1:302213/251‑1 (MQ=255)       tGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTc                                                                                                                                                                                                                                        >  1:656016/1‑250 (MQ=255)        gTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTc                                                                                                                                                                                                                                        >  1:438572/1‑249 (MQ=255)        gTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTc                                                                                                                                                                                                                                        >  1:140449/1‑249 (MQ=255)        gTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTc                                                                                                                                                                                                                                        >  1:154193/1‑249 (MQ=255)               gAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTg                                                                                                                                                                                                                                <  1:330505/250‑1 (MQ=255)               gAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGa                                                                                                                                                                                                                               >  1:642764/1‑251 (MQ=255)                 gTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTcc                                                                                                                                                                                                                                       <  1:802823/241‑1 (MQ=255)                      aTTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGt                                                                                                                                                                                                                         >  1:446201/1‑250 (MQ=255)                       ttCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCg                                                                                                                                                                                                                       <  1:836032/251‑1 (MQ=255)                         cAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGtt                                                                                                                                                                                                                     >  1:699490/1‑251 (MQ=255)                           aTGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAg                                                                                                                                                                                                                   >  1:879607/1‑251 (MQ=255)                            tGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGc                                                                                                                                                                                                                  <  1:242638/251‑1 (MQ=255)                             gTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGAt                                                                                                                                                                                                                              >  1:509186/1‑238 (MQ=255)                                  gcTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTcc                                                                                                                                                                                                                                       >  1:86715/1‑224 (MQ=255)                                       aTGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCAt                                                                                                                                                                                                                                                 <  1:808258/209‑1 (MQ=255)                                       aTGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATa                                                                                                                                                                                                        >  1:129862/1‑250 (MQ=255)                                       aTGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATa                                                                                                                                                                                                        >  1:675479/1‑250 (MQ=255)                                       aTGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATAc                                                                                                                                                                                                       <  1:99952/251‑1 (MQ=255)                                              cGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCAGTTGGTCAGCTCGCGATTGGCGTTTTCCACGACGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCAc                                                                                                                                                                                                                <  1:319442/235‑1 (MQ=255)                                                  aCGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATAc                                                                                                                                                                                                       >  1:306925/1‑240 (MQ=255)                                                         atatCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGGAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAAt                                                                                                                                                                                     >  1:424747/1‑251 (MQ=255)                                                           atCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCg                                                                                                                                                                                   >  1:577095/1‑251 (MQ=255)                                                            tCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCgg                                                                                                                                                                                  >  1:316056/1‑251 (MQ=255)                                                            tCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTCCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCgg                                                                                                                                                                                  >  1:198797/1‑251 (MQ=255)                                                               aTAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTg                                                                                                                                                                                <  1:283226/250‑1 (MQ=255)                                                                 aaGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGaaa                                                                                                                                                                             >  1:398492/1‑251 (MQ=255)                                                                   gttgttCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAAtt                                                                                                                                                                           >  1:547941/1‑251 (MQ=255)                                                                   gttgttCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAAtt                                                                                                                                                                           >  1:297469/1‑251 (MQ=255)                                                                   gttgttCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAAtt                                                                                                                                                                           >  1:677011/1‑251 (MQ=255)                                                                      gttCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTaaaa                                                                                                                                                                                                          >  1:21131/1‑217 (MQ=255)                                                                           aCCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATaa                                                                                                                                                                   >  1:31472/1‑251 (MQ=255)                                                                           aCCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATaa                                                                                                                                                                   >  1:101030/1‑251 (MQ=255)                                                                            ccTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGAt                                                                                                                                                                                                                              <  1:741216/191‑1 (MQ=255)                                                                            ccTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAAc                                                                                                                                                                  <  1:311071/251‑1 (MQ=255)                                                                            ccTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAAc                                                                                                                                                                  <  1:163121/251‑1 (MQ=255)                                                                                   aTCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAg                                                                                                                                                                                                >  1:194856/1‑214 (MQ=255)                                                                                        aTTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAAttg                                                                                                                                                      <  1:118867/251‑1 (MQ=255)                                                                                           cGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATc                                                                                                                                                                                    >  1:655500/1‑218 (MQ=255)                                                                                                     tttGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGt                                                                                                                                         <  1:827057/251‑1 (MQ=255)                                                                                                     tttGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGt                                                                                                                                         <  1:825536/251‑1 (MQ=255)                                                                                                                        tCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAg                                                                                                                                                                                                <  1:488764/177‑1 (MQ=255)                                                                                                                             aaaTTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCaaa                                                                                                                 >  1:588769/1‑251 (MQ=255)                                                                                                                               aTTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAg                                                                                                                             <  1:177954/237‑1 (MQ=255)                                                                                                                               aTTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAAt                                                                                                                >  1:670915/1‑250 (MQ=255)                                                                                                                                         ggcTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCGCAAATCGGCGACCTg                                                                                                      >  1:642651/1‑250 (MQ=255)                                                                                                                                         ggcTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTg                                                                                                      >  1:121103/1‑250 (MQ=255)                                                                                                                                          gcTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTggg                                                                                                    >  1:569973/1‑249 (MQ=255)                                                                                                                                           cTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGcat                                                                                                   <  1:297347/251‑1 (MQ=255)                                                                                                                                             tGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGcatca                                                                                                 <  1:690014/251‑1 (MQ=255)                                                                                                                                                 cGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATgg                                                                                              >  1:801861/1‑250 (MQ=255)                                                                                                                                                   ttGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGtt                                                                                                                                        <  1:185938/206‑1 (MQ=255)                                                                                                                                                           gTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAg                                                                                                                                                                                                <  1:496071/142‑1 (MQ=255)                                                                                                                                                             cccAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGCAAGATCGTTgg                                                                                  >  1:287509/1‑250 (MQ=255)                                                                                                                                                                                   aTTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGCAAGATCGTTGGCGCCGTCGCCGATGGCAACGgtg                                                           >  1:228348/1‑251 (MQ=255)                                                                                                                                                                                           tttCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGCAAGATCGTTGGCGCCGTCGCCGATGGCAACGGTGTTTTcggc                                                   <  1:312086/251‑1 (MQ=255)                                                                                                                                                                                            ttCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGCAAGa                                                                                         <  1:4199/212‑1 (MQ=255)                                                                                                                                                                                                   gCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAg                                                                                                                             <  1:273615/169‑1 (MQ=255)                                                                                                                                                                                                     gTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAg                                                                                                                                                                                                >  1:356385/1‑100 (MQ=255)                                                                                                                                                                                                                cGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGCAAGATCGTTGGCGCCGTCGCCGATGGCAACGGTGTTTTCGGCGGCAATGTGAAATTGTTgcg                               >  1:743688/1‑250 (MQ=255)                                                                                                                                                                                                                     ccGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGCAAGATCGTTGGCGCCGTCGCCGATGGCAACGGTGTTTTCGGCGGCAATGTGAAATTGTTGCGCAAgtt                         >  1:334225/1‑251 (MQ=255)                                                                                                                                                                                                                          gATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATaaa                                                                                                                                                                                                  >  1:881204/1‑77 (MQ=255)                                                                                                                                                                                                                          gATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAAc                                                                                                                                                                  >  1:471232/1‑109 (MQ=255)                                                                                                                                                                                                                          gATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGc                                                                                             <  1:702258/178‑1 (MQ=255)                                                                                                                                                                                                                           aTTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGCAAGATCGTTGGCGCCGTCGCCGATGGCAACGGTGTTTTCGGCGGCAATGTGAAATTGTTGCGCAAGTTGTTGTa                   <  1:626820/251‑1 (MQ=255)                                                                                                                                                                                                                              tCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGCAAGATCGTTGGCGCCGTCGCCGATGGCAACGGTGTTTTCGGCGGCAATGTGAAATTGTTGCGCAAGTTGTTGTAAGa                <  1:482459/251‑1 (MQ=255)                                                                                                                                                                                                                              tCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGCAAGATCGTTGGCGCCGTCGCCGATGGCAACGGTGTTTTCGGCGGCAATGTGAAATTGTTGCGCAAGTTGTTGTAAGa                <  1:863024/251‑1 (MQ=255)                                                                                                                                                                                                                                  aaaCAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATaaa                                                                                                                                                                                                  <  1:732441/69‑1 (MQ=255)                                                                                                                                                                                                                                        tGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGCAAGATCGTTGGCGCCGTCGCCGATGGCAACGGTGTTTTCGGCGGCAATGTGAAATTGTTGCGCAAGTTGTTGTAAGATTTGCGCttt      >  1:704487/1‑251 (MQ=255)                                                                                                                                                                                                                                         gTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCataat                                                                                                                               >  1:73827/1‑129 (MQ=255)                                                                                                                                                                                                                                         gTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGCAAGAt                                                                                        <  1:213106/168‑1 (MQ=255)                                                                                                                                                                                                                                             aaTGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGCAAGATCGTTGGCGCCGTCGCCGATGGCAACGGTGTTTTCGGCGGCAATGTGAAATTGTTGCGCAAGTTGTTGTAAGATTTGCGCTTTGTAt  <  1:863420/250‑1 (MQ=255)                                                                                                                                                                                                                                             aaTGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGCAAGATCGTTGGCGCCGTCGCCGATGGCAACGGTGTTTTCGGCGGCAATGTGAAATTGTTGCGCAAGTTGTTGTAAGATTTGCGCTTTGTAt  <  1:384283/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    CGGGAATGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATTGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGCAAGATCGTTGGCGCCGTCGCCGATGGCAACGGTGTTTTCGGCGGCAATGTGAAATTGTTGCGCAAGTTGTTGTAAGATTTGCGCTTTGTAT  >  1/627736‑628221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑