Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 604,628 A→G intergenic (‑13/‑130) unknown ← / → unknown Lysine decarboxylase family/Cytochrome c‑type biogenesis protein DsbD, protein‑disulfide reductase (EC 1.8.1.8)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1604,6280AG100.0% 255.2 / NA 72intergenic (‑13/‑130)unknown/unknownLysine decarboxylase family/Cytochrome c‑type biogenesis protein DsbD, protein‑disulfide reductase (EC 1.8.1.8)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/34);  total (38/34)

CGGTACAACCTTGGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAATTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCGTTTTATGAAAAGATTTCTCCTCATTATCCTCTGCTTTTTCGTGACATTCAATGTGCAAGCCGTGGATCTGTTTAACCAAAAGCCAAAATTT  >  1/604381‑604865
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             
cGGTACAACCTTGTTGTCCCAATC‑CCCGCCCCTGAAAAAATTCAGGGATCACGCCGATACCGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAgtta                                                                                                                                                                                                                                            <  1:778811/251‑1 (MQ=255)
  ggtacaccttgggtgtcccattccccGCGCCTGAAAAATTTCGGGGACCCCGCCGATACCGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTGACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAgtta                                                                                                                                                                                                                                            <  1:381256/227‑1 (MQ=255)
   aaaaaCCTGGGTG‑ACCAATTCCCCGCCCCTGTAAAAATCCGGGAACCACGCCAAAAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTAttt                                                                                                                                                                                                                                         <  1:818925/248‑1 (MQ=255)
        cttgggTGTACCATTC‑CCCGCCCCTGAAAAAATTCAGGAACCACGCCGATAACGTTCCCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCAt                                                                                                                                                                                                                                    <  1:237460/247‑1 (MQ=255)
             gtgtACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATa                                                                                                                                                                                                                               >  1:361389/1‑251 (MQ=255)
                             cgcCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAAt                                                                                                                                                                                                                >  1:699206/1‑251 (MQ=255)
                                  gaaaaaaTTCGGGGACCCCGCCGAAAACGGTCCCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAAttt                                                                                                                                                                                                                          <  1:243481/234‑1 (MQ=255)
                                  aaaaaatttcggggccccggcggATAACGGTCCCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCat                                                                                                                                                                                                           <  1:284782/229‑1 (MQ=255)
                                   aaaaaaTTCGGGGACCACGCCGATACCGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCat                                                                                                                                                                                                           <  1:303632/249‑1 (MQ=255)
                                              ggacccccccaaTAACGTTCCCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATaa                                                                                                                                                                                                  <  1:719183/237‑1 (MQ=255)
                                                               gTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATaa                                                                                                                                                                               >  1:470545/1‑250 (MQ=255)
                                                               gTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATaa                                                                                                                                                                               >  1:420898/1‑250 (MQ=255)
                                                                tACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATaac                                                                                                                                                                              <  1:485789/250‑1 (MQ=255)
                                                                 cccgccgTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATaacaa                                                                                                                                                                            <  1:760868/250‑1 (MQ=255)
                                                                     ccgTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATaaca                                                                                                                                                                             >  1:574863/1‑246 (MQ=255)
                                                                          ggAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAg                                                                                                                                                                   >  1:633271/1‑251 (MQ=255)
                                                                           gAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAg                                                                                                                                                                   <  1:122155/250‑1 (MQ=255)
                                                                                 aCCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATaacaa                                                                                                                                                                            <  1:774095/235‑1 (MQ=255)
                                                                                      aTCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGt                                                                                                                                                                                   >  1:183879/1‑223 (MQ=255)
                                                                                       tCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTg                                                                                                                                                       >  1:471507/1‑250 (MQ=255)
                                                                                          gCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTgcag                                                                                                                                                    >  1:830367/1‑250 (MQ=255)
                                                                                                acGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGc                                                                                                                                                                  <  1:610448/230‑1 (MQ=255)
                                                                                                   cccATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAatttatt                                                                                                                                           <  1:89645/250‑1 (MQ=255)
                                                                                                                  tttCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGaa                                                                                                                           <  1:347738/251‑1 (MQ=255)
                                                                                                                  tttCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGaa                                                                                                                           <  1:162387/251‑1 (MQ=255)
                                                                                                                  tttCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGaa                                                                                                                           <  1:440542/251‑1 (MQ=255)
                                                                                                                  tttCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGaa                                                                                                                           <  1:811501/251‑1 (MQ=255)
                                                                                                                   ttCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAAt                                                                                                                          <  1:179978/251‑1 (MQ=255)
                                                                                                                          gccATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTc                                                                                                                                                                                                              >  1:815473/1‑160 (MQ=255)
                                                                                                                          gccATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCaa                                                                                                                    >  1:721816/1‑250 (MQ=255)
                                                                                                                               aaaCTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAgtta                                                                                                                                                                                                                                            >  1:6571/1‑125 (MQ=255)
                                                                                                                               aaaCTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGatat                                                                                                                                                           >  1:326767/1‑206 (MQ=255)
                                                                                                                               aaaCTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGatat                                                                                                                                                           >  1:269819/1‑206 (MQ=255)
                                                                                                                               aaaCTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACAtttt                                                                                                              >  1:263427/1‑251 (MQ=255)
                                                                                                                               aaaCTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACAtttt                                                                                                              >  1:99436/1‑251 (MQ=255)
                                                                                                                                aaCTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCACAACAATAGGTATaacaac                                                                                                                                                                           >  1:433436/1‑189 (MQ=255)
                                                                                                                                   tAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATgg                                                                                                          <  1:712403/251‑1 (MQ=255)
                                                                                                                                    aaCGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATaacaa                                                                                                                                                                            >  1:471848/1‑184 (MQ=255)
                                                                                                                                     aCGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGta                                                                                                                                                                                  >  1:280109/1‑177 (MQ=255)
                                                                                                                                        tATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGAt                                                                                                     >  1:626214/1‑251 (MQ=255)
                                                                                                                                          tGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATaa                                                                                                   >  1:659913/1‑251 (MQ=255)
                                                                                                                                            tCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATaaca                                                                                                 >  1:791590/1‑251 (MQ=255)
                                                                                                                                            tCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATaac                                                                                                  <  1:247350/250‑1 (MQ=255)
                                                                                                                                                   tGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGaa                                                                                                                           >  1:108250/1‑218 (MQ=255)
                                                                                                                                                    gCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGaa                                                                                                                           <  1:231525/217‑1 (MQ=255)
                                                                                                                                                    gCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACAttt                                                                                                               <  1:613325/229‑1 (MQ=255)
                                                                                                                                                    gCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATaacaac                                                                                               >  1:790365/1‑245 (MQ=255)
                                                                                                                                                    gCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCGtttt                                                                                         >  1:831316/1‑251 (MQ=255)
                                                                                                                                                    gCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCGtttt                                                                                         >  1:634407/1‑251 (MQ=255)
                                                                                                                                                           ccATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAattt                                                                                                                                              >  1:7167/1‑191 (MQ=255)
                                                                                                                                                           ccATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGaa                                                                                                                           <  1:818058/210‑1 (MQ=255)
                                                                                                                                                                cGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGc                                                                                                                                                                  <  1:502137/166‑1 (MQ=255)
                                                                                                                                                                  ccTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATaacaac                                                                                               <  1:394255/231‑1 (MQ=255)
                                                                                                                                                                      aTTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATaacaac                                                                                               >  1:381322/1‑227 (MQ=255)
                                                                                                                                                                          aaTAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCGTTTTATGAAAAGATTTCTCCTCATTa                                                                   >  1:144374/1‑251 (MQ=255)
                                                                                                                                                                              gTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCGTTTTATGAAAAGATTTCTCCTCATTATc                                                                 >  1:338630/1‑249 (MQ=255)
                                                                                                                                                                                cACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCGTTTTATGAAAAGATTTCTCCTCATTATCct                                                               >  1:714889/1‑249 (MQ=255)
                                                                                                                                                                                     ggCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCg                                                                                             <  1:244718/214‑1 (MQ=255)
                                                                                                                                                                                      gCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCGTTTTATGAAAAGATTTCTCCTCATTATCCTCTGCtttt                                                       >  1:440874/1‑251 (MQ=255)
                                                                                                                                                                                         aTAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCGTTTTATAAAAAGATTTCTCCTCATTATCCTCTGCTTTTTCg                                                    <  1:287009/251‑1 (MQ=255)
                                                                                                                                                                                                      ttGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCGTTTTATGAAAAGATTTCTCCTCATTATCCTCTGCTTTTTCGTGACATTCAAtgt                                       >  1:7865/1‑251 (MQ=255)
                                                                                                                                                                                                        gTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCGTTTTATGAAAAGATTTCTCCTCATTATCCTCTGCTTTTTCGTGACATTCAAtgtg                                      >  1:117380/1‑250 (MQ=255)
                                                                                                                                                                                                              tGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCGTTTTATGAAAAGATTTCTCCTCATTATCCTCTGCTTTTTCGTGACATTCAATGTGCAAGCCg                               <  1:827933/251‑1 (MQ=255)
                                                                                                                                                                                                                     ccACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGa                                                                                                                                                                                                      <  1:325566/77‑1 (MQ=255)
                                                                                                                                                                                                                     ccACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATg                                                                                                                         <  1:818722/154‑1 (MQ=255)
                                                                                                                                                                                                                       cagaTAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATcc                                                                                                                                                                                              >  1:158341/4‑82 (MQ=255)
                                                                                                                                                                                                                        caATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGaa                                                                                                                           <  1:565893/149‑1 (MQ=255)
                                                                                                                                                                                                                              aCCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCGTTTTATGAAAAGATTTCTCCTCATTATCCTCTGCTTTTTCGTGACATTCAATGTGCAAGCCGTGGATCTGTTTAACCa               <  1:675222/251‑1 (MQ=255)
                                                                                                                                                                                                                               ccACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCGTTTTATGAAAAGATTTCTCCTCATTATCCTCTGCTTTTTCGTGACATTCAATGTGCAAGCCGTGGATCTGTTTAACCaa              >  1:781180/1‑251 (MQ=255)
                                                                                                                                                                                                                                 acaATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCg                                                                                             <  1:359621/170‑1 (MQ=255)
                                                                                                                                                                                                                                    aTGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCGTTTTa                                                                                        >  1:559199/1‑172 (MQ=255)
                                                                                                                                                                                                                                      gTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATg                                                                                                                         <  1:547862/137‑1 (MQ=255)
                                                                                                                                                                                                                                      gTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCGTTTTATGAAAAGATTTCTCCTCATTATCCTCTGCTTTTTCGTGACATTCAATGTGCAAGCCGTGGATCTGTTTAACCAAAAGCCaa       >  1:409852/1‑251 (MQ=255)
                                                                                                                                                                                                                                      gTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCGTTTTATGAAAAGATTTCTCCTCATTATCCTCTGCTTTTTCGTGACATTCAATGTGCAAGCCGTGGATCTGTTTAACCAAAAGCCaa       >  1:260830/1‑251 (MQ=255)
                                                                                                                                                                                                                                           tttGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCGTTTTATGAAAAGATTTCTCCTCATTATCCTCTGCTTTTTCGTGACATTCAATGTGCAAGCCGTGGATCTGTTTAACCAAAAGCCAAAAttt  <  1:373294/251‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             
CGGTACAACCTTGGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAATTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAATGAGCAACATTTTATGGAAGATAACAACCGTTTTATGAAAAGATTTCTCCTCATTATCCTCTGCTTTTTCGTGACATTCAATGTGCAAGCCGTGGATCTGTTTAACCAAAAGCCAAAATTT  >  1/604381‑604865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑